Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates
Cell morphology, protein/DNA ratio, ganglioside analysis, taurine uptake, and the activity of neurone specific enolase showed that neuronal cells mature faster when grown on polyethyleneimine coated plates compared to cells grown on polylysine coated plates. Our results also show higher protein/DNA...
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| Veröffentlicht in: | Journal of neuroscience research 1992-08, Vol.32 (4), p.562-568 |
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| container_title | Journal of neuroscience research |
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| creator | Lelong, Isabelle H. Petegnief, V. Rebel, G. |
| description | Cell morphology, protein/DNA ratio, ganglioside analysis, taurine uptake, and the activity of neurone specific enolase showed that neuronal cells mature faster when grown on polyethyleneimine coated plates compared to cells grown on polylysine coated plates. Our results also show higher protein/DNA ratio and total and neuron specific enolase activities in cells grown in serum supplemented medium, when compared to their counterparts grown in synthetic medium. Moreover, our results show that only some specific markers can be used to determine the early and late events of cell maturation, whereas other markers continuously vary with time. © 1992 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jnr.490320411 |
| format | Article |
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Neuroglia ; maturation ; neurons ; Neurons - physiology ; Phosphopyruvate Hydratase - metabolism ; Polyethyleneimine ; Polylysine ; Proteins - metabolism ; Taurine - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience research, 1992-08, Vol.32 (4), p.562-568</ispartof><rights>Copyright © 1992 Wiley‐Liss, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4691-df6d1af692b3d9abd6e3e04fce60aa3442b3a6244e2088bdc2c8bc8b155077f73</citedby><cites>FETCH-LOGICAL-c4691-df6d1af692b3d9abd6e3e04fce60aa3442b3a6244e2088bdc2c8bc8b155077f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.490320411$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.490320411$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5500226$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1527802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lelong, Isabelle H.</creatorcontrib><creatorcontrib>Petegnief, V.</creatorcontrib><creatorcontrib>Rebel, G.</creatorcontrib><title>Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>Cell morphology, protein/DNA ratio, ganglioside analysis, taurine uptake, and the activity of neurone specific enolase showed that neuronal cells mature faster when grown on polyethyleneimine coated plates compared to cells grown on polylysine coated plates. Our results also show higher protein/DNA ratio and total and neuron specific enolase activities in cells grown in serum supplemented medium, when compared to their counterparts grown in synthetic medium. Moreover, our results show that only some specific markers can be used to determine the early and late events of cell maturation, whereas other markers continuously vary with time. © 1992 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Catecholamines - metabolism</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Chromatography, Thin Layer</subject><subject>Culture Media</subject><subject>cultures</subject><subject>Cytological Techniques</subject><subject>DNA - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gangliosides - metabolism</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>maturation</subject><subject>neurons</subject><subject>Neurons - physiology</subject><subject>Phosphopyruvate Hydratase - metabolism</subject><subject>Polyethyleneimine</subject><subject>Polylysine</subject><subject>Proteins - metabolism</subject><subject>Taurine - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EKkvhyBEpB9Rb2vFH7OSISmmpyiJB-biZiTNRU5xksROV_Htc7bIgISFZGsnzePz6MWPPORxzAHFyO4RjVYEUoDh_wFYcKpOrQpmHbAVSQ66Ai8fsSYy3AFBVhTxgB7wQpgSxYt_WNIdxQJ858j5mPU5zoKzFOFHIxiHbjH6h6WbxNFDXdwNlbsSJmmzjU4nZdIPDb84v8R_gKXvUoo_0bFcP2ac3Z9enF_nV-_O3p6-ucqd0xfOm1Q3HVleilk2FdaNJEqjWkQZEqVTaRy2UIgFlWTdOuLJOixcFGNMaeciOtnM3YfwxU5xs38X7N-FA4xytSYJKw0UC8y3owhhjoNZuQtdjWCwHe2_UJqN2bzTxL3aD57qn5g-9VZj6L3d9jA59G3BwXdxjKR8IoRNmtthd52n5_532cv3h7wC7wF36k5_7kxi-W22kKeyX9bk16uP1u89fS_ta_gLyx5-F</recordid><startdate>199208</startdate><enddate>199208</enddate><creator>Lelong, Isabelle H.</creator><creator>Petegnief, V.</creator><creator>Rebel, G.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199208</creationdate><title>Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates</title><author>Lelong, Isabelle H. ; Petegnief, V. ; Rebel, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4691-df6d1af692b3d9abd6e3e04fce60aa3442b3a6244e2088bdc2c8bc8b155077f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Catecholamines - metabolism</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>Chromatography, Thin Layer</topic><topic>Culture Media</topic><topic>cultures</topic><topic>Cytological Techniques</topic><topic>DNA - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gangliosides - metabolism</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>maturation</topic><topic>neurons</topic><topic>Neurons - physiology</topic><topic>Phosphopyruvate Hydratase - metabolism</topic><topic>Polyethyleneimine</topic><topic>Polylysine</topic><topic>Proteins - metabolism</topic><topic>Taurine - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lelong, Isabelle H.</creatorcontrib><creatorcontrib>Petegnief, V.</creatorcontrib><creatorcontrib>Rebel, G.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lelong, Isabelle H.</au><au>Petegnief, V.</au><au>Rebel, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J. Neurosci. Res</addtitle><date>1992-08</date><risdate>1992</risdate><volume>32</volume><issue>4</issue><spage>562</spage><epage>568</epage><pages>562-568</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><coden>JNREDK</coden><abstract>Cell morphology, protein/DNA ratio, ganglioside analysis, taurine uptake, and the activity of neurone specific enolase showed that neuronal cells mature faster when grown on polyethyleneimine coated plates compared to cells grown on polylysine coated plates. Our results also show higher protein/DNA ratio and total and neuron specific enolase activities in cells grown in serum supplemented medium, when compared to their counterparts grown in synthetic medium. 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| ispartof | Journal of neuroscience research, 1992-08, Vol.32 (4), p.562-568 |
| issn | 0360-4012 1097-4547 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Biological and medical sciences Catecholamines - metabolism Cells, Cultured Chick Embryo Chromatography, Thin Layer Culture Media cultures Cytological Techniques DNA - metabolism Fundamental and applied biological sciences. Psychology Gangliosides - metabolism Isolated neuron and nerve. Neuroglia maturation neurons Neurons - physiology Phosphopyruvate Hydratase - metabolism Polyethyleneimine Polylysine Proteins - metabolism Taurine - metabolism Vertebrates: nervous system and sense organs |
| title | Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates |
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