Immunocytochemical demonstration of dopamine-beta-hydroxylase and cytochrome B561 on the axonal reticulum in bovine sympathetic neurons
In sympathetic neurons the axonal reticulum can be considered an extension of the secretory pole of the Golgi apparatus. If this tubular system indeed represents the neurosecretory apparatus, it would likely contain on its membranes the enzymes involved in catecholamine synthesis. To test this hypot...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1992-10, Vol.40 (10), p.1599-1604 |
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| creator | Quatacker, JR Annaert, WG Miserez, BJ De Potter, WP |
| description | In sympathetic neurons the axonal reticulum can be considered an extension of the secretory pole of the Golgi apparatus. If this tubular system indeed represents the neurosecretory apparatus, it would likely contain on its membranes the enzymes involved in catecholamine synthesis. To test this hypothesis, we investigated the distribution of dopamine-beta-hydroxylase and cytochrome b561 in bovine splenic nerve and nerve terminals in the vas deferens with an immunogold procedure after glycolmethacrylate embedding. Counterstaining with phosphotungstic acid at low pH selectively revealed the axonal reticulum elements. With antibodies against both enzymes, gold labeling was observed over the large dense-cored vesicles, the Golgi-associated axonal reticulum, the reticulum within axons, and the tubular complex at the nerve terminal. From our results it can be concluded that in sympathetic neurons the axonal reticulum represents a tubular neurosecretory system, extending from the Golgi apparatus in the cell soma to the nerve terminal. This concept emphasizes the local production of neurosecretory vesicles and may be of importance in the interpretation of neuronal transmission in normal and diseased states. |
| doi_str_mv | 10.1177/40.10.1527378 |
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If this tubular system indeed represents the neurosecretory apparatus, it would likely contain on its membranes the enzymes involved in catecholamine synthesis. To test this hypothesis, we investigated the distribution of dopamine-beta-hydroxylase and cytochrome b561 in bovine splenic nerve and nerve terminals in the vas deferens with an immunogold procedure after glycolmethacrylate embedding. Counterstaining with phosphotungstic acid at low pH selectively revealed the axonal reticulum elements. With antibodies against both enzymes, gold labeling was observed over the large dense-cored vesicles, the Golgi-associated axonal reticulum, the reticulum within axons, and the tubular complex at the nerve terminal. From our results it can be concluded that in sympathetic neurons the axonal reticulum represents a tubular neurosecretory system, extending from the Golgi apparatus in the cell soma to the nerve terminal. This concept emphasizes the local production of neurosecretory vesicles and may be of importance in the interpretation of neuronal transmission in normal and diseased states.</description><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Axons - enzymology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cattle</subject><subject>Cytochrome b Group - metabolism</subject><subject>Dopamine beta-Hydroxylase - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Neurons - enzymology</subject><subject>Phosphotungstic Acid - chemistry</subject><subject>Sympathetic Nervous System - cytology</subject><subject>Sympathetic Nervous System - enzymology</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtv1TAQhS1EVS6FJUskLxBISAE_E2cJFY9KldjA2nKcSeMqti92wm1-AX-7Lrm0q0qWZ-TzzRnrIPSKkg-UNs1HUWo5kjW8UU_QjkpJK0mEeIp2hDBWlQfxDD3P-ZoQKoRUp-j0iO_Q3wvvlxDtOkc7gnfWTLgHH0Oek5ldDDgOuI97412AqoPZVOPap3izTiYDNqHH22yKHvBnWVNcZuaxSDcxFLMEs7PLtHjsAu7in2KD8-r3pjBFwQGWVLa9QCeDmTK8PNYz9Ovrl5_n36vLH98uzj9dVlYwPldN2yg1dGqo65pyKLdkNbStIr1UhIMceks7C8CNokwNQ8t6VnemZqY2wBg_Q283332KvxfIs_YuW5gmEyAuWTecESVqWcBqA22KOScY9D45b9KqKdF3wWtB_rVbkoV_fTReOg_9A32vvznqJpeQh2SCdfkeE1wpRu_Wvt-wbK5AX8cllRDzozvfbfDorsaDS6CzN9NUfkD14XD4D7ctvwUC36c-</recordid><startdate>19921001</startdate><enddate>19921001</enddate><creator>Quatacker, JR</creator><creator>Annaert, WG</creator><creator>Miserez, BJ</creator><creator>De Potter, WP</creator><general>Histochemical Soc</general><general>SAGE Publications</general><general>Histochemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921001</creationdate><title>Immunocytochemical demonstration of dopamine-beta-hydroxylase and cytochrome B561 on the axonal reticulum in bovine sympathetic neurons</title><author>Quatacker, JR ; Annaert, WG ; Miserez, BJ ; De Potter, WP</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-79788fb8f66613e666526e9980d5803e5fdc1bcee3a8128ff92d26ba62a6ae223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Axons - enzymology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cattle</topic><topic>Cytochrome b Group - metabolism</topic><topic>Dopamine beta-Hydroxylase - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Neurons - enzymology</topic><topic>Phosphotungstic Acid - chemistry</topic><topic>Sympathetic Nervous System - cytology</topic><topic>Sympathetic Nervous System - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Quatacker, JR</creatorcontrib><creatorcontrib>Annaert, WG</creatorcontrib><creatorcontrib>Miserez, BJ</creatorcontrib><creatorcontrib>De Potter, WP</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Quatacker, JR</au><au>Annaert, WG</au><au>Miserez, BJ</au><au>De Potter, WP</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunocytochemical demonstration of dopamine-beta-hydroxylase and cytochrome B561 on the axonal reticulum in bovine sympathetic neurons</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>1992-10-01</date><risdate>1992</risdate><volume>40</volume><issue>10</issue><spage>1599</spage><epage>1604</epage><pages>1599-1604</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><coden>JHCYAS</coden><abstract>In sympathetic neurons the axonal reticulum can be considered an extension of the secretory pole of the Golgi apparatus. If this tubular system indeed represents the neurosecretory apparatus, it would likely contain on its membranes the enzymes involved in catecholamine synthesis. To test this hypothesis, we investigated the distribution of dopamine-beta-hydroxylase and cytochrome b561 in bovine splenic nerve and nerve terminals in the vas deferens with an immunogold procedure after glycolmethacrylate embedding. Counterstaining with phosphotungstic acid at low pH selectively revealed the axonal reticulum elements. With antibodies against both enzymes, gold labeling was observed over the large dense-cored vesicles, the Golgi-associated axonal reticulum, the reticulum within axons, and the tubular complex at the nerve terminal. From our results it can be concluded that in sympathetic neurons the axonal reticulum represents a tubular neurosecretory system, extending from the Golgi apparatus in the cell soma to the nerve terminal. 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| subjects | Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals Axons - enzymology Biological and medical sciences Blotting, Western Cattle Cytochrome b Group - metabolism Dopamine beta-Hydroxylase - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Immunohistochemistry Neurons - enzymology Phosphotungstic Acid - chemistry Sympathetic Nervous System - cytology Sympathetic Nervous System - enzymology |
| title | Immunocytochemical demonstration of dopamine-beta-hydroxylase and cytochrome B561 on the axonal reticulum in bovine sympathetic neurons |
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