Further evidence for the presence of γ-aminobutyric acidA (GABAA) receptors on the cytoplasmic side of Deiter's membrane
1. The permeation of labeled Cl- across single nerve membranes microdissected from rabbit Deiters' neurons was studied in a microchamber system. The in---out permeation of the ions was evaluated under control conditions and in the presence of either 10(-6) M GABA or 10(-6) M GABA plus 10(-5) M...
Gespeichert in:
| Veröffentlicht in: | Cellular and molecular neurobiology 1992-08, Vol.12 (4), p.327-332 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 332 |
|---|---|
| container_issue | 4 |
| container_start_page | 327 |
| container_title | Cellular and molecular neurobiology |
| container_volume | 12 |
| creator | RAPALLINO, M. V CUPELLO, A HYDEN, H |
| description | 1. The permeation of labeled Cl- across single nerve membranes microdissected from rabbit Deiters' neurons was studied in a microchamber system. The in---out permeation of the ions was evaluated under control conditions and in the presence of either 10(-6) M GABA or 10(-6) M GABA plus 10(-5) M bicuculline methiodide (BMI) on the membrane cytoplasmic side. 2. In 32 experiments, involving one animal each, at least two membranes served as controls and at least two others were studied with the addition of GABA. Within each experiment all the membranes were obtained from the same animal. 3. In an additional 10 experiments, involving one animal each, at least two membranes served as controls and at least two others were studied in the presence of GABA plus bicuculline methiodide on the membrane cytoplasmic side. 4. The data show that 10(-6) M GABA on the Deiters' membrane cytoplasmic side stimulates Cl- permeation in---out by 42% (P = 0.0000001). When 10(-5) M BMI was present together with GABA, no stimulation of Cl- in---out permeation occurred. |
| doi_str_mv | 10.1007/BF00734933 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_73208297</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73208297</sourcerecordid><originalsourceid>FETCH-LOGICAL-p235t-691090b076399c915fdc3ffdc1ca1cce911c1008a33056e92ef4ca54f06abe6c3</originalsourceid><addsrcrecordid>eNo9UE1LxDAUDKKs6-rFu5CD-HGoJk3TNsfu6q7Cghc9lzR9wUi7qUkr9Hf5P_xNRi1e3oOZecO8QeiUkhtKSHa7XIfJEsHYHppTnrEozRnZR3MSZ3GUsIQcoiPv3wghghA-QzPK4oyzeI7G9eD6V3AYPkwNOwVYW4cDgjsH_hewGn99RrI1O1sN_eiMwlKZusBXm2JZFNfYgYKut85ju_s9VWNvu0b6Nkh9sP2xuAPTg7v0uIW2cnIHx-hAy8bDybQX6GV9_7x6iLZPm8dVsY26mPE-SgUNoSuSpUwIJSjXtWI6DKokVQoEpSqUkEvGCE9BxKATJXmiSSorSBVboIs_387Z9wF8X7bGK2iakMEOvsxYTPJYZEF4NgmHqoW67JxppRvLqavAn0-89Eo2OjyhjP-XcZ6kOc_ZN1xQeAI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73208297</pqid></control><display><type>article</type><title>Further evidence for the presence of γ-aminobutyric acidA (GABAA) receptors on the cytoplasmic side of Deiter's membrane</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>RAPALLINO, M. V ; CUPELLO, A ; HYDEN, H</creator><creatorcontrib>RAPALLINO, M. V ; CUPELLO, A ; HYDEN, H</creatorcontrib><description>1. The permeation of labeled Cl- across single nerve membranes microdissected from rabbit Deiters' neurons was studied in a microchamber system. The in---out permeation of the ions was evaluated under control conditions and in the presence of either 10(-6) M GABA or 10(-6) M GABA plus 10(-5) M bicuculline methiodide (BMI) on the membrane cytoplasmic side. 2. In 32 experiments, involving one animal each, at least two membranes served as controls and at least two others were studied with the addition of GABA. Within each experiment all the membranes were obtained from the same animal. 3. In an additional 10 experiments, involving one animal each, at least two membranes served as controls and at least two others were studied in the presence of GABA plus bicuculline methiodide on the membrane cytoplasmic side. 4. The data show that 10(-6) M GABA on the Deiters' membrane cytoplasmic side stimulates Cl- permeation in---out by 42% (P = 0.0000001). When 10(-5) M BMI was present together with GABA, no stimulation of Cl- in---out permeation occurred.</description><identifier>ISSN: 0272-4340</identifier><identifier>EISSN: 1573-6830</identifier><identifier>DOI: 10.1007/BF00734933</identifier><identifier>PMID: 1327532</identifier><identifier>CODEN: CMNEDI</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Animals ; Bicuculline - analogs & derivatives ; Bicuculline - pharmacology ; Biological and medical sciences ; Cell Membrane - chemistry ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Chloride Channels ; Chlorides - metabolism ; Cytoplasm - chemistry ; Fundamental and applied biological sciences. Psychology ; gamma-Aminobutyric Acid - pharmacology ; Membrane Proteins - drug effects ; Rabbits ; Receptors, GABA-A - analysis ; Receptors, GABA-A - drug effects ; Vertebrates: nervous system and sense organs ; Vestibular Nuclei - metabolism</subject><ispartof>Cellular and molecular neurobiology, 1992-08, Vol.12 (4), p.327-332</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5546858$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1327532$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RAPALLINO, M. V</creatorcontrib><creatorcontrib>CUPELLO, A</creatorcontrib><creatorcontrib>HYDEN, H</creatorcontrib><title>Further evidence for the presence of γ-aminobutyric acidA (GABAA) receptors on the cytoplasmic side of Deiter's membrane</title><title>Cellular and molecular neurobiology</title><addtitle>Cell Mol Neurobiol</addtitle><description>1. The permeation of labeled Cl- across single nerve membranes microdissected from rabbit Deiters' neurons was studied in a microchamber system. The in---out permeation of the ions was evaluated under control conditions and in the presence of either 10(-6) M GABA or 10(-6) M GABA plus 10(-5) M bicuculline methiodide (BMI) on the membrane cytoplasmic side. 2. In 32 experiments, involving one animal each, at least two membranes served as controls and at least two others were studied with the addition of GABA. Within each experiment all the membranes were obtained from the same animal. 3. In an additional 10 experiments, involving one animal each, at least two membranes served as controls and at least two others were studied in the presence of GABA plus bicuculline methiodide on the membrane cytoplasmic side. 4. The data show that 10(-6) M GABA on the Deiters' membrane cytoplasmic side stimulates Cl- permeation in---out by 42% (P = 0.0000001). When 10(-5) M BMI was present together with GABA, no stimulation of Cl- in---out permeation occurred.</description><subject>Animals</subject><subject>Bicuculline - analogs & derivatives</subject><subject>Bicuculline - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - chemistry</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Chloride Channels</subject><subject>Chlorides - metabolism</subject><subject>Cytoplasm - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>Membrane Proteins - drug effects</subject><subject>Rabbits</subject><subject>Receptors, GABA-A - analysis</subject><subject>Receptors, GABA-A - drug effects</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Vestibular Nuclei - metabolism</subject><issn>0272-4340</issn><issn>1573-6830</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UE1LxDAUDKKs6-rFu5CD-HGoJk3TNsfu6q7Cghc9lzR9wUi7qUkr9Hf5P_xNRi1e3oOZecO8QeiUkhtKSHa7XIfJEsHYHppTnrEozRnZR3MSZ3GUsIQcoiPv3wghghA-QzPK4oyzeI7G9eD6V3AYPkwNOwVYW4cDgjsH_hewGn99RrI1O1sN_eiMwlKZusBXm2JZFNfYgYKut85ju_s9VWNvu0b6Nkh9sP2xuAPTg7v0uIW2cnIHx-hAy8bDybQX6GV9_7x6iLZPm8dVsY26mPE-SgUNoSuSpUwIJSjXtWI6DKokVQoEpSqUkEvGCE9BxKATJXmiSSorSBVboIs_387Z9wF8X7bGK2iakMEOvsxYTPJYZEF4NgmHqoW67JxppRvLqavAn0-89Eo2OjyhjP-XcZ6kOc_ZN1xQeAI</recordid><startdate>19920801</startdate><enddate>19920801</enddate><creator>RAPALLINO, M. V</creator><creator>CUPELLO, A</creator><creator>HYDEN, H</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19920801</creationdate><title>Further evidence for the presence of γ-aminobutyric acidA (GABAA) receptors on the cytoplasmic side of Deiter's membrane</title><author>RAPALLINO, M. V ; CUPELLO, A ; HYDEN, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-691090b076399c915fdc3ffdc1ca1cce911c1008a33056e92ef4ca54f06abe6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Bicuculline - analogs & derivatives</topic><topic>Bicuculline - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - chemistry</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Chloride Channels</topic><topic>Chlorides - metabolism</topic><topic>Cytoplasm - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - pharmacology</topic><topic>Membrane Proteins - drug effects</topic><topic>Rabbits</topic><topic>Receptors, GABA-A - analysis</topic><topic>Receptors, GABA-A - drug effects</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Vestibular Nuclei - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RAPALLINO, M. V</creatorcontrib><creatorcontrib>CUPELLO, A</creatorcontrib><creatorcontrib>HYDEN, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular and molecular neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RAPALLINO, M. V</au><au>CUPELLO, A</au><au>HYDEN, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Further evidence for the presence of γ-aminobutyric acidA (GABAA) receptors on the cytoplasmic side of Deiter's membrane</atitle><jtitle>Cellular and molecular neurobiology</jtitle><addtitle>Cell Mol Neurobiol</addtitle><date>1992-08-01</date><risdate>1992</risdate><volume>12</volume><issue>4</issue><spage>327</spage><epage>332</epage><pages>327-332</pages><issn>0272-4340</issn><eissn>1573-6830</eissn><coden>CMNEDI</coden><abstract>1. The permeation of labeled Cl- across single nerve membranes microdissected from rabbit Deiters' neurons was studied in a microchamber system. The in---out permeation of the ions was evaluated under control conditions and in the presence of either 10(-6) M GABA or 10(-6) M GABA plus 10(-5) M bicuculline methiodide (BMI) on the membrane cytoplasmic side. 2. In 32 experiments, involving one animal each, at least two membranes served as controls and at least two others were studied with the addition of GABA. Within each experiment all the membranes were obtained from the same animal. 3. In an additional 10 experiments, involving one animal each, at least two membranes served as controls and at least two others were studied in the presence of GABA plus bicuculline methiodide on the membrane cytoplasmic side. 4. The data show that 10(-6) M GABA on the Deiters' membrane cytoplasmic side stimulates Cl- permeation in---out by 42% (P = 0.0000001). When 10(-5) M BMI was present together with GABA, no stimulation of Cl- in---out permeation occurred.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>1327532</pmid><doi>10.1007/BF00734933</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0272-4340 |
| ispartof | Cellular and molecular neurobiology, 1992-08, Vol.12 (4), p.327-332 |
| issn | 0272-4340 1573-6830 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73208297 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Bicuculline - analogs & derivatives Bicuculline - pharmacology Biological and medical sciences Cell Membrane - chemistry Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Chloride Channels Chlorides - metabolism Cytoplasm - chemistry Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - pharmacology Membrane Proteins - drug effects Rabbits Receptors, GABA-A - analysis Receptors, GABA-A - drug effects Vertebrates: nervous system and sense organs Vestibular Nuclei - metabolism |
| title | Further evidence for the presence of γ-aminobutyric acidA (GABAA) receptors on the cytoplasmic side of Deiter's membrane |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T00%3A21%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Further%20evidence%20for%20the%20presence%20of%20%CE%B3-aminobutyric%20acidA%20(GABAA)%20receptors%20on%20the%20cytoplasmic%20side%20of%20Deiter's%20membrane&rft.jtitle=Cellular%20and%20molecular%20neurobiology&rft.au=RAPALLINO,%20M.%20V&rft.date=1992-08-01&rft.volume=12&rft.issue=4&rft.spage=327&rft.epage=332&rft.pages=327-332&rft.issn=0272-4340&rft.eissn=1573-6830&rft.coden=CMNEDI&rft_id=info:doi/10.1007/BF00734933&rft_dat=%3Cproquest_pubme%3E73208297%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73208297&rft_id=info:pmid/1327532&rfr_iscdi=true |