Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay
Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that non-specific esterases from normal monocytes can be determined b...
Gespeichert in:
| Veröffentlicht in: | The hematology journal : the official journal of the European Haematology Association 2003, Vol.4 (1), p.26-30 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 30 |
|---|---|
| container_issue | 1 |
| container_start_page | 26 |
| container_title | The hematology journal : the official journal of the European Haematology Association |
| container_volume | 4 |
| creator | da Fonseca, Luiz M Brunetti, Iguatemy L Campa, Ana Catalani, Luiz H Calado, Rodrigo T Falcão, Roberto P |
| description | Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that non-specific esterases from normal monocytes can be determined by a chemiluminescent method. In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories.
Bone marrow samples were obtained from 66 patients with AML (M0, two cases; M1, 12 cases; M2, 13 cases; M3, 10 cases; M4, 11 cases; M5, 12 cases; M6, two cases; M7, four cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured for 10 min. Two parameters were assessed, the peak (PLE) and the integrated light emission (ILE).
Both PLE and ILE were higher in FAB-M4 and FAB-M5 subtypes compared to other AML subtypes (P |
| doi_str_mv | 10.1038/sj.thj.6200209 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73208203</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73208203</sourcerecordid><originalsourceid>FETCH-LOGICAL-c206t-53e72e0c9be6ab5ee3401c4ceb59d83cae4427da6ae84e7d96a552fc58941a633</originalsourceid><addsrcrecordid>eNpFkM1Pg0AQxTdGY7V69Wg4eYPuN3BsGr-SJl70vFmWIQVZtjJw4L-3pCQ9zZuZ37xMHiFPjCaMimyDTTIcmkRzSjnNr8gdk6mOlRb8etZaxzLTdEXuERtKmWYpvSUrxnXOFUvvyLRFBEQP3RCFKvKhC24aahe54I-hm8d1F1k3DhD5CdpwIWxXXvjNrIrW4rxpYfwFX1uMiimykTucmnb0dQfoZkeLaKcHclPZFuFxqWvy8_b6vfuI91_vn7vtPnac6iFWAlIO1OUFaFsoACEpc9JBofIyE86ClDwtrbaQSUjLXFuleOVUlktmtRBr8nL2PfbhbwQcjK9Pb7St7SCMaFLBacbpDCZn0PUBsYfKHPva234yjJo5bIONOYVtlrBPB8-L81h4KC_4kq74B9QXf6s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73208203</pqid></control><display><type>article</type><title>Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>da Fonseca, Luiz M ; Brunetti, Iguatemy L ; Campa, Ana ; Catalani, Luiz H ; Calado, Rodrigo T ; Falcão, Roberto P</creator><creatorcontrib>da Fonseca, Luiz M ; Brunetti, Iguatemy L ; Campa, Ana ; Catalani, Luiz H ; Calado, Rodrigo T ; Falcão, Roberto P</creatorcontrib><description>Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that non-specific esterases from normal monocytes can be determined by a chemiluminescent method. In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories.
Bone marrow samples were obtained from 66 patients with AML (M0, two cases; M1, 12 cases; M2, 13 cases; M3, 10 cases; M4, 11 cases; M5, 12 cases; M6, two cases; M7, four cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured for 10 min. Two parameters were assessed, the peak (PLE) and the integrated light emission (ILE).
Both PLE and ILE were higher in FAB-M4 and FAB-M5 subtypes compared to other AML subtypes (P<0.001). In addition, the classification of AML cases into FAB-M4, FAB-M5 and nonmonocytic subtypes based on ILE analysis was concordant with alpha-naphthyl acetate esterase (ANAE) in 97% of cases (kappa coefficient 0.94, P<0.001).
These findings indicate that this chemiluminescent assay was able to determine the monocytic component of FAB-M4 and FAB-M5 cells, and the classification of AML subtypes based on chemiluminescent analysis strongly agreed with the cytochemical ANAE-staining. In conclusion, this chemiluminescent assay is a simple, fast and objective method, which may be useful as an alternative tool in the differential diagnosis of AML subtypes.</description><identifier>ISSN: 1466-4860</identifier><identifier>EISSN: 1476-5632</identifier><identifier>DOI: 10.1038/sj.thj.6200209</identifier><identifier>PMID: 12692517</identifier><language>eng</language><publisher>England</publisher><subject>Acute Disease ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Benzoates - metabolism ; Bone Marrow - pathology ; Carboxylesterase ; Carboxylic Ester Hydrolases - analysis ; Child ; Child, Preschool ; Cryopreservation ; Diagnosis, Differential ; Female ; Horseradish Peroxidase - metabolism ; Humans ; Leukemia, Monocytic, Acute - diagnosis ; Leukemia, Monocytic, Acute - enzymology ; Leukemia, Monocytic, Acute - pathology ; Leukemia, Myeloid - pathology ; Leukemia, Myelomonocytic, Acute - diagnosis ; Leukemia, Myelomonocytic, Acute - enzymology ; Leukemia, Myelomonocytic, Acute - pathology ; Luminescent Measurements ; Male ; Middle Aged ; Monocytes - enzymology ; Monocytes - pathology ; Neoplasm Proteins - analysis ; Neoplastic Stem Cells - enzymology ; Neoplastic Stem Cells - pathology ; Sodium Fluoride - pharmacology</subject><ispartof>The hematology journal : the official journal of the European Haematology Association, 2003, Vol.4 (1), p.26-30</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c206t-53e72e0c9be6ab5ee3401c4ceb59d83cae4427da6ae84e7d96a552fc58941a633</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12692517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Fonseca, Luiz M</creatorcontrib><creatorcontrib>Brunetti, Iguatemy L</creatorcontrib><creatorcontrib>Campa, Ana</creatorcontrib><creatorcontrib>Catalani, Luiz H</creatorcontrib><creatorcontrib>Calado, Rodrigo T</creatorcontrib><creatorcontrib>Falcão, Roberto P</creatorcontrib><title>Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay</title><title>The hematology journal : the official journal of the European Haematology Association</title><addtitle>Hematol J</addtitle><description>Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that non-specific esterases from normal monocytes can be determined by a chemiluminescent method. In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories.
Bone marrow samples were obtained from 66 patients with AML (M0, two cases; M1, 12 cases; M2, 13 cases; M3, 10 cases; M4, 11 cases; M5, 12 cases; M6, two cases; M7, four cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured for 10 min. Two parameters were assessed, the peak (PLE) and the integrated light emission (ILE).
Both PLE and ILE were higher in FAB-M4 and FAB-M5 subtypes compared to other AML subtypes (P<0.001). In addition, the classification of AML cases into FAB-M4, FAB-M5 and nonmonocytic subtypes based on ILE analysis was concordant with alpha-naphthyl acetate esterase (ANAE) in 97% of cases (kappa coefficient 0.94, P<0.001).
These findings indicate that this chemiluminescent assay was able to determine the monocytic component of FAB-M4 and FAB-M5 cells, and the classification of AML subtypes based on chemiluminescent analysis strongly agreed with the cytochemical ANAE-staining. In conclusion, this chemiluminescent assay is a simple, fast and objective method, which may be useful as an alternative tool in the differential diagnosis of AML subtypes.</description><subject>Acute Disease</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Benzoates - metabolism</subject><subject>Bone Marrow - pathology</subject><subject>Carboxylesterase</subject><subject>Carboxylic Ester Hydrolases - analysis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cryopreservation</subject><subject>Diagnosis, Differential</subject><subject>Female</subject><subject>Horseradish Peroxidase - metabolism</subject><subject>Humans</subject><subject>Leukemia, Monocytic, Acute - diagnosis</subject><subject>Leukemia, Monocytic, Acute - enzymology</subject><subject>Leukemia, Monocytic, Acute - pathology</subject><subject>Leukemia, Myeloid - pathology</subject><subject>Leukemia, Myelomonocytic, Acute - diagnosis</subject><subject>Leukemia, Myelomonocytic, Acute - enzymology</subject><subject>Leukemia, Myelomonocytic, Acute - pathology</subject><subject>Luminescent Measurements</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Monocytes - enzymology</subject><subject>Monocytes - pathology</subject><subject>Neoplasm Proteins - analysis</subject><subject>Neoplastic Stem Cells - enzymology</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Sodium Fluoride - pharmacology</subject><issn>1466-4860</issn><issn>1476-5632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1Pg0AQxTdGY7V69Wg4eYPuN3BsGr-SJl70vFmWIQVZtjJw4L-3pCQ9zZuZ37xMHiFPjCaMimyDTTIcmkRzSjnNr8gdk6mOlRb8etZaxzLTdEXuERtKmWYpvSUrxnXOFUvvyLRFBEQP3RCFKvKhC24aahe54I-hm8d1F1k3DhD5CdpwIWxXXvjNrIrW4rxpYfwFX1uMiimykTucmnb0dQfoZkeLaKcHclPZFuFxqWvy8_b6vfuI91_vn7vtPnac6iFWAlIO1OUFaFsoACEpc9JBofIyE86ClDwtrbaQSUjLXFuleOVUlktmtRBr8nL2PfbhbwQcjK9Pb7St7SCMaFLBacbpDCZn0PUBsYfKHPva234yjJo5bIONOYVtlrBPB8-L81h4KC_4kq74B9QXf6s</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>da Fonseca, Luiz M</creator><creator>Brunetti, Iguatemy L</creator><creator>Campa, Ana</creator><creator>Catalani, Luiz H</creator><creator>Calado, Rodrigo T</creator><creator>Falcão, Roberto P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay</title><author>da Fonseca, Luiz M ; Brunetti, Iguatemy L ; Campa, Ana ; Catalani, Luiz H ; Calado, Rodrigo T ; Falcão, Roberto P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c206t-53e72e0c9be6ab5ee3401c4ceb59d83cae4427da6ae84e7d96a552fc58941a633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Acute Disease</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Benzoates - metabolism</topic><topic>Bone Marrow - pathology</topic><topic>Carboxylesterase</topic><topic>Carboxylic Ester Hydrolases - analysis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cryopreservation</topic><topic>Diagnosis, Differential</topic><topic>Female</topic><topic>Horseradish Peroxidase - metabolism</topic><topic>Humans</topic><topic>Leukemia, Monocytic, Acute - diagnosis</topic><topic>Leukemia, Monocytic, Acute - enzymology</topic><topic>Leukemia, Monocytic, Acute - pathology</topic><topic>Leukemia, Myeloid - pathology</topic><topic>Leukemia, Myelomonocytic, Acute - diagnosis</topic><topic>Leukemia, Myelomonocytic, Acute - enzymology</topic><topic>Leukemia, Myelomonocytic, Acute - pathology</topic><topic>Luminescent Measurements</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Monocytes - enzymology</topic><topic>Monocytes - pathology</topic><topic>Neoplasm Proteins - analysis</topic><topic>Neoplastic Stem Cells - enzymology</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Sodium Fluoride - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>da Fonseca, Luiz M</creatorcontrib><creatorcontrib>Brunetti, Iguatemy L</creatorcontrib><creatorcontrib>Campa, Ana</creatorcontrib><creatorcontrib>Catalani, Luiz H</creatorcontrib><creatorcontrib>Calado, Rodrigo T</creatorcontrib><creatorcontrib>Falcão, Roberto P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The hematology journal : the official journal of the European Haematology Association</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Fonseca, Luiz M</au><au>Brunetti, Iguatemy L</au><au>Campa, Ana</au><au>Catalani, Luiz H</au><au>Calado, Rodrigo T</au><au>Falcão, Roberto P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay</atitle><jtitle>The hematology journal : the official journal of the European Haematology Association</jtitle><addtitle>Hematol J</addtitle><date>2003</date><risdate>2003</risdate><volume>4</volume><issue>1</issue><spage>26</spage><epage>30</epage><pages>26-30</pages><issn>1466-4860</issn><eissn>1476-5632</eissn><abstract>Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that non-specific esterases from normal monocytes can be determined by a chemiluminescent method. In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories.
Bone marrow samples were obtained from 66 patients with AML (M0, two cases; M1, 12 cases; M2, 13 cases; M3, 10 cases; M4, 11 cases; M5, 12 cases; M6, two cases; M7, four cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured for 10 min. Two parameters were assessed, the peak (PLE) and the integrated light emission (ILE).
Both PLE and ILE were higher in FAB-M4 and FAB-M5 subtypes compared to other AML subtypes (P<0.001). In addition, the classification of AML cases into FAB-M4, FAB-M5 and nonmonocytic subtypes based on ILE analysis was concordant with alpha-naphthyl acetate esterase (ANAE) in 97% of cases (kappa coefficient 0.94, P<0.001).
These findings indicate that this chemiluminescent assay was able to determine the monocytic component of FAB-M4 and FAB-M5 cells, and the classification of AML subtypes based on chemiluminescent analysis strongly agreed with the cytochemical ANAE-staining. In conclusion, this chemiluminescent assay is a simple, fast and objective method, which may be useful as an alternative tool in the differential diagnosis of AML subtypes.</abstract><cop>England</cop><pmid>12692517</pmid><doi>10.1038/sj.thj.6200209</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-4860 |
| ispartof | The hematology journal : the official journal of the European Haematology Association, 2003, Vol.4 (1), p.26-30 |
| issn | 1466-4860 1476-5632 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73208203 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Acute Disease Adolescent Adult Aged Aged, 80 and over Benzoates - metabolism Bone Marrow - pathology Carboxylesterase Carboxylic Ester Hydrolases - analysis Child Child, Preschool Cryopreservation Diagnosis, Differential Female Horseradish Peroxidase - metabolism Humans Leukemia, Monocytic, Acute - diagnosis Leukemia, Monocytic, Acute - enzymology Leukemia, Monocytic, Acute - pathology Leukemia, Myeloid - pathology Leukemia, Myelomonocytic, Acute - diagnosis Leukemia, Myelomonocytic, Acute - enzymology Leukemia, Myelomonocytic, Acute - pathology Luminescent Measurements Male Middle Aged Monocytes - enzymology Monocytes - pathology Neoplasm Proteins - analysis Neoplastic Stem Cells - enzymology Neoplastic Stem Cells - pathology Sodium Fluoride - pharmacology |
| title | Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T02%3A50%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assessment%20of%20monocytic%20component%20in%20acute%20myelomonocytic%20and%20monocytic/monoblastic%20leukemias%20by%20a%20chemiluminescent%20assay&rft.jtitle=The%20hematology%20journal%20:%20the%20official%20journal%20of%20the%20European%20Haematology%20Association&rft.au=da%20Fonseca,%20Luiz%20M&rft.date=2003&rft.volume=4&rft.issue=1&rft.spage=26&rft.epage=30&rft.pages=26-30&rft.issn=1466-4860&rft.eissn=1476-5632&rft_id=info:doi/10.1038/sj.thj.6200209&rft_dat=%3Cproquest_cross%3E73208203%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73208203&rft_id=info:pmid/12692517&rfr_iscdi=true |