Cloning and expression of a heme binding protein from the genome of Saccharomyces cerevisiae

The YLR205c gene of Saccharomyces cerevisiae does not show significant sequence identity to any known gene, except for heme oxygenase (22% to human HO-1). The YLR205 ORF was cloned and overexpressed in both Escherichia coli and S. cerevisiae. Both expression systems yielded proteins that bound heme...

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Veröffentlicht in:	Protein expression and purification 2003-04, Vol.28 (2), p.340-349
Hauptverfasser:	Auclair, Karine, Huang, Hong-Wei, Moënne-Loccoz, Pierre, Ortiz de Montellano, Paul R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Cloning, Molecular Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Gene Expression Genome, Fungal Hemeproteins - genetics Hemeproteins - metabolism Molecular Sequence Data Protein Binding Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Sequence Alignment Sequence Homology, Amino Acid
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