Endometrial lymphoid tissue in the timed endometrial biopsy: Morphometric and immunohistochemical aspects
OBJECTIVES: The purpose of this study was to provide a morphometric profile of endometrial granulated lymphocytes and to investigate qualitative and quantitative differences in leukocyte subsets in precisely timed luteal phase endometrial biopsies. STUDY DESIGN: Endometrial biopsies were obtained fr...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1992-09, Vol.167 (3), p.667-674 |
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| creator | Klentzeris, Lucas D. Bulmer, Judith N. Warren, Alistair Morrison, Lynn Li, Tin-Chin Cooke, Ian D. |
| description | OBJECTIVES: The purpose of this study was to provide a morphometric profile of endometrial granulated lymphocytes and to investigate qualitative and quantitative differences in leukocyte subsets in precisely timed luteal phase endometrial biopsies.
STUDY DESIGN: Endometrial biopsies were obtained from 24 normal fertile women at 4, 7, 10, and 13 days after the luteinizing hormone surge. Endometrial granulated lymphocytes were assessed morphometrically in 2 μm resin sections. Eleven monoclonal antibodies were used to characterize leukocytes in frozen sections. Semiquantitation was performed with a Quantimet 970 image analyzer. Data were analyzed with one-way analysis of variance.
RESULTS: CD8+ (T suppressor-cytotoxic) cells increased significantly from 4 to 7 days after the luteinizing hormone surge, whereas CD68+ macrophages increased from days 10 to 13. Lymphocytes with an unusual phenotype (CD56+, CD38+, CD2+) increased dramatically after 7 days. The volume fraction of endometrium occupied by the nuclei of endometrial granulated lymphocytes did not alter, but their mean nuclear diameter and axial ratio decreased from days 7 to 13.
CONCLUSION: The morphometric findings indicate in situ proliferation of endometrial granulated lymphocytes rather than migration from the peripheral circulation. T lymphocytes, macrophages, and endometrial granulated lymphocytes increase significantly between certain stages of the luteal phase. |
| doi_str_mv | 10.1016/S0002-9378(11)91568-3 |
| format | Article |
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STUDY DESIGN: Endometrial biopsies were obtained from 24 normal fertile women at 4, 7, 10, and 13 days after the luteinizing hormone surge. Endometrial granulated lymphocytes were assessed morphometrically in 2 μm resin sections. Eleven monoclonal antibodies were used to characterize leukocytes in frozen sections. Semiquantitation was performed with a Quantimet 970 image analyzer. Data were analyzed with one-way analysis of variance.
RESULTS: CD8+ (T suppressor-cytotoxic) cells increased significantly from 4 to 7 days after the luteinizing hormone surge, whereas CD68+ macrophages increased from days 10 to 13. Lymphocytes with an unusual phenotype (CD56+, CD38+, CD2+) increased dramatically after 7 days. The volume fraction of endometrium occupied by the nuclei of endometrial granulated lymphocytes did not alter, but their mean nuclear diameter and axial ratio decreased from days 7 to 13.
CONCLUSION: The morphometric findings indicate in situ proliferation of endometrial granulated lymphocytes rather than migration from the peripheral circulation. T lymphocytes, macrophages, and endometrial granulated lymphocytes increase significantly between certain stages of the luteal phase.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/S0002-9378(11)91568-3</identifier><identifier>PMID: 1530020</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>Philadelphia, PA: Mosby, Inc</publisher><subject>Adult ; Antigens, CD - analysis ; Biological and medical sciences ; Biopsy - methods ; endometrial biopsy ; endometrial granulated lymphocytes ; Endometrial leukocyte subpopulations ; Endometrium - metabolism ; Endometrium - pathology ; Female ; Granulocytes - immunology ; Granulocytes - pathology ; Humans ; Immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; Lymphocyte Subsets - pathology ; Lymphocytes - immunology ; Lymphocytes - pathology ; Lymphoid Tissue - metabolism ; Lymphoid Tissue - pathology ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Pregnancy ; Time Factors</subject><ispartof>American journal of obstetrics and gynecology, 1992-09, Vol.167 (3), p.667-674</ispartof><rights>1992 Mosby</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-ee871138dcb3aa768b2bdabc4d5ac1a2459927df9cd15fbca93cd3f184c9c64b3</citedby><cites>FETCH-LOGICAL-c538t-ee871138dcb3aa768b2bdabc4d5ac1a2459927df9cd15fbca93cd3f184c9c64b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0002937811915683$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4372502$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1530020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klentzeris, Lucas D.</creatorcontrib><creatorcontrib>Bulmer, Judith N.</creatorcontrib><creatorcontrib>Warren, Alistair</creatorcontrib><creatorcontrib>Morrison, Lynn</creatorcontrib><creatorcontrib>Li, Tin-Chin</creatorcontrib><creatorcontrib>Cooke, Ian D.</creatorcontrib><title>Endometrial lymphoid tissue in the timed endometrial biopsy: Morphometric and immunohistochemical aspects</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>OBJECTIVES: The purpose of this study was to provide a morphometric profile of endometrial granulated lymphocytes and to investigate qualitative and quantitative differences in leukocyte subsets in precisely timed luteal phase endometrial biopsies.
STUDY DESIGN: Endometrial biopsies were obtained from 24 normal fertile women at 4, 7, 10, and 13 days after the luteinizing hormone surge. Endometrial granulated lymphocytes were assessed morphometrically in 2 μm resin sections. Eleven monoclonal antibodies were used to characterize leukocytes in frozen sections. Semiquantitation was performed with a Quantimet 970 image analyzer. Data were analyzed with one-way analysis of variance.
RESULTS: CD8+ (T suppressor-cytotoxic) cells increased significantly from 4 to 7 days after the luteinizing hormone surge, whereas CD68+ macrophages increased from days 10 to 13. Lymphocytes with an unusual phenotype (CD56+, CD38+, CD2+) increased dramatically after 7 days. The volume fraction of endometrium occupied by the nuclei of endometrial granulated lymphocytes did not alter, but their mean nuclear diameter and axial ratio decreased from days 7 to 13.
CONCLUSION: The morphometric findings indicate in situ proliferation of endometrial granulated lymphocytes rather than migration from the peripheral circulation. T lymphocytes, macrophages, and endometrial granulated lymphocytes increase significantly between certain stages of the luteal phase.</description><subject>Adult</subject><subject>Antigens, CD - analysis</subject><subject>Biological and medical sciences</subject><subject>Biopsy - methods</subject><subject>endometrial biopsy</subject><subject>endometrial granulated lymphocytes</subject><subject>Endometrial leukocyte subpopulations</subject><subject>Endometrium - metabolism</subject><subject>Endometrium - pathology</subject><subject>Female</subject><subject>Granulocytes - immunology</subject><subject>Granulocytes - pathology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lymphocyte Subsets - pathology</subject><subject>Lymphocytes - immunology</subject><subject>Lymphocytes - pathology</subject><subject>Lymphoid Tissue - metabolism</subject><subject>Lymphoid Tissue - pathology</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Pregnancy</subject><subject>Time Factors</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQRi1EVS6FR6iUBUKwSPHEcWKzqVBVoFIrFsDacsYTXaMkDnaCdN8e3x8Vdl3Z4zljW-dj7BL4FXBoPnznnFelFq16B_Beg2xUKZ6xDXDdlo1q1HO2eUResJcp_dqXla7O2TlIkfd8w_zt5MJIS_R2KIbdOG-Dd8XiU1qp8FOxbClXI7mC_gM7H-a0-1g8hJgHDqdY2MkVfhzXKWx9WgJuafSYYZtmwiW9Yme9HRK9Pq0X7Ofn2x83X8v7b1_ubj7dlyiFWkoi1QII5bAT1raN6qrO2Q5rJy2CrWqpddW6XqMD2XdotUAnelA1amzqTlywt8d75xh-r5QWM_qENAx2orAm04qKi1arJ0Foai1BQgblEcQYUorUmzn60cadAW72WZhDFmYv2gCYQxZG5LnL0wNrlw3-mzrKz_03p75NWVQf7YQ-PWK1aCvJq4xdHzHK1v54iiahpwnJ-ZjFGhf8Ex_5C8bkqJ4</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Klentzeris, Lucas D.</creator><creator>Bulmer, Judith N.</creator><creator>Warren, Alistair</creator><creator>Morrison, Lynn</creator><creator>Li, Tin-Chin</creator><creator>Cooke, Ian D.</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19920901</creationdate><title>Endometrial lymphoid tissue in the timed endometrial biopsy: Morphometric and immunohistochemical aspects</title><author>Klentzeris, Lucas D. ; Bulmer, Judith N. ; Warren, Alistair ; Morrison, Lynn ; Li, Tin-Chin ; Cooke, Ian D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-ee871138dcb3aa768b2bdabc4d5ac1a2459927df9cd15fbca93cd3f184c9c64b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adult</topic><topic>Antigens, CD - analysis</topic><topic>Biological and medical sciences</topic><topic>Biopsy - methods</topic><topic>endometrial biopsy</topic><topic>endometrial granulated lymphocytes</topic><topic>Endometrial leukocyte subpopulations</topic><topic>Endometrium - metabolism</topic><topic>Endometrium - pathology</topic><topic>Female</topic><topic>Granulocytes - immunology</topic><topic>Granulocytes - pathology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Lymphocyte Subsets - pathology</topic><topic>Lymphocytes - immunology</topic><topic>Lymphocytes - pathology</topic><topic>Lymphoid Tissue - metabolism</topic><topic>Lymphoid Tissue - pathology</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Pregnancy</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klentzeris, Lucas D.</creatorcontrib><creatorcontrib>Bulmer, Judith N.</creatorcontrib><creatorcontrib>Warren, Alistair</creatorcontrib><creatorcontrib>Morrison, Lynn</creatorcontrib><creatorcontrib>Li, Tin-Chin</creatorcontrib><creatorcontrib>Cooke, Ian D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klentzeris, Lucas D.</au><au>Bulmer, Judith N.</au><au>Warren, Alistair</au><au>Morrison, Lynn</au><au>Li, Tin-Chin</au><au>Cooke, Ian D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endometrial lymphoid tissue in the timed endometrial biopsy: Morphometric and immunohistochemical aspects</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>167</volume><issue>3</issue><spage>667</spage><epage>674</epage><pages>667-674</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>OBJECTIVES: The purpose of this study was to provide a morphometric profile of endometrial granulated lymphocytes and to investigate qualitative and quantitative differences in leukocyte subsets in precisely timed luteal phase endometrial biopsies.
STUDY DESIGN: Endometrial biopsies were obtained from 24 normal fertile women at 4, 7, 10, and 13 days after the luteinizing hormone surge. Endometrial granulated lymphocytes were assessed morphometrically in 2 μm resin sections. Eleven monoclonal antibodies were used to characterize leukocytes in frozen sections. Semiquantitation was performed with a Quantimet 970 image analyzer. Data were analyzed with one-way analysis of variance.
RESULTS: CD8+ (T suppressor-cytotoxic) cells increased significantly from 4 to 7 days after the luteinizing hormone surge, whereas CD68+ macrophages increased from days 10 to 13. Lymphocytes with an unusual phenotype (CD56+, CD38+, CD2+) increased dramatically after 7 days. The volume fraction of endometrium occupied by the nuclei of endometrial granulated lymphocytes did not alter, but their mean nuclear diameter and axial ratio decreased from days 7 to 13.
CONCLUSION: The morphometric findings indicate in situ proliferation of endometrial granulated lymphocytes rather than migration from the peripheral circulation. T lymphocytes, macrophages, and endometrial granulated lymphocytes increase significantly between certain stages of the luteal phase.</abstract><cop>Philadelphia, PA</cop><pub>Mosby, Inc</pub><pmid>1530020</pmid><doi>10.1016/S0002-9378(11)91568-3</doi><tpages>8</tpages></addata></record> |
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| ispartof | American journal of obstetrics and gynecology, 1992-09, Vol.167 (3), p.667-674 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Antigens, CD - analysis Biological and medical sciences Biopsy - methods endometrial biopsy endometrial granulated lymphocytes Endometrial leukocyte subpopulations Endometrium - metabolism Endometrium - pathology Female Granulocytes - immunology Granulocytes - pathology Humans Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Lymphocyte Subsets - pathology Lymphocytes - immunology Lymphocytes - pathology Lymphoid Tissue - metabolism Lymphoid Tissue - pathology Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pregnancy Time Factors |
| title | Endometrial lymphoid tissue in the timed endometrial biopsy: Morphometric and immunohistochemical aspects |
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