Detection and characterization of feline Bartonella henselae in the Czech Republic
The aims of the study were to characterize isolates of Bartonella henselae and to determine the prevalence of bacteremic domestic cats in urban and suburban parts of Prague, Czech Republic. Five (18%) gram-negative fastidious bacterial single-cat isolates were recovered from 27 hemocultures incubate...
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| Veröffentlicht in: | Veterinary microbiology 2003-05, Vol.93 (3), p.261-273 |
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| creator | Melter, O Hercı́k, K Weyant, R.S Janeček, J Nemec, A Mecera, J Gonzorová, L’ Branny, P |
| description | The aims of the study were to characterize isolates of
Bartonella henselae and to determine the prevalence of bacteremic domestic cats in urban and suburban parts of Prague, Czech Republic. Five (18%) gram-negative fastidious bacterial single-cat isolates were recovered from 27 hemocultures incubated without previous freezing. Four of these isolates originated from flea infested stray cats (
n=6) and one from a shelter cat without any ectoparasites (
n=21). None of the 34 previously frozen specimens from flea free pet cats yielded any bacteria. All five isolates were catalase and oxidase negative. Their enzymatic activity, RFLP profile of citrate synthetase gene (
gltA) and DNA–DNA hybridization results were typical of
B. henselae. According to their
PvuII and
BglI ribotypes the isolates could be allocated to two homogeneous groups. Ribotype
HindIII and RFLP of 16S–23S rRNA spacer region analysis gave unique profiles different from those of
Bartonella quintana,
Bartonella elizabethae and
Bartonella clarridgeiae. The 16S rRNA type-specific amplification revealed an identical profile typical of
B. henselae genotype II for all the cat isolates studied. Pulsed-field gel electrophoresis (PFGE) assigned a different profile to each of the isolates studied.
Determination of the enzymatic activity, RFLP of
gltA gene, RFLP of 16S–23S rRNA spacer region, and
HindIII ribotype could be efficient tools for identification of
B. henselae isolates. Ribotyping (
PvuII,
BglI), 16S rRNA typing and PFGE may be useful methods to prospect ecology and epidemiology of the agent. |
| doi_str_mv | 10.1016/S0378-1135(03)00032-4 |
| format | Article |
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Bartonella henselae and to determine the prevalence of bacteremic domestic cats in urban and suburban parts of Prague, Czech Republic. Five (18%) gram-negative fastidious bacterial single-cat isolates were recovered from 27 hemocultures incubated without previous freezing. Four of these isolates originated from flea infested stray cats (
n=6) and one from a shelter cat without any ectoparasites (
n=21). None of the 34 previously frozen specimens from flea free pet cats yielded any bacteria. All five isolates were catalase and oxidase negative. Their enzymatic activity, RFLP profile of citrate synthetase gene (
gltA) and DNA–DNA hybridization results were typical of
B. henselae. According to their
PvuII and
BglI ribotypes the isolates could be allocated to two homogeneous groups. Ribotype
HindIII and RFLP of 16S–23S rRNA spacer region analysis gave unique profiles different from those of
Bartonella quintana,
Bartonella elizabethae and
Bartonella clarridgeiae. The 16S rRNA type-specific amplification revealed an identical profile typical of
B. henselae genotype II for all the cat isolates studied. Pulsed-field gel electrophoresis (PFGE) assigned a different profile to each of the isolates studied.
Determination of the enzymatic activity, RFLP of
gltA gene, RFLP of 16S–23S rRNA spacer region, and
HindIII ribotype could be efficient tools for identification of
B. henselae isolates. Ribotyping (
PvuII,
BglI), 16S rRNA typing and PFGE may be useful methods to prospect ecology and epidemiology of the agent.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(03)00032-4</identifier><identifier>PMID: 12695049</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; bacteria ; Bacteriology ; Bartonella clarridgeiae ; Bartonella elizabethae ; Bartonella henselae ; Bartonella henselae - enzymology ; Bartonella henselae - genetics ; Bartonella henselae - isolation & purification ; Bartonella Infections - enzymology ; Bartonella Infections - epidemiology ; Bartonella Infections - microbiology ; Bartonella Infections - veterinary ; Bartonella quintana ; Biological and medical sciences ; Cat ; Cat Diseases - epidemiology ; Cat Diseases - microbiology ; catalase ; Catalase - metabolism ; Cats ; Citrate (si)-Synthase - chemistry ; Citrate (si)-Synthase - genetics ; citrates ; Czech Republic ; Czech Republic - epidemiology ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal Spacer - chemistry ; DNA, Ribosomal Spacer - genetics ; ectoparasites ; Electrophoresis, Gel, Pulsed-Field - veterinary ; enzyme activity ; Epidemiology ; Female ; freezing ; Fundamental and applied biological sciences. Psychology ; genes ; hybridization ; Male ; Microbiology ; Molecular typing ; Nucleic Acid Hybridization ; Oxidoreductases - metabolism ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; pulsed-field gel electrophoresis ; ribosomal RNA ; ribotypes ; Ribotyping - veterinary ; RNA, Ribosomal, 16S - chemistry ; RNA, Ribosomal, 16S - genetics ; Siphonaptera</subject><ispartof>Veterinary microbiology, 2003-05, Vol.93 (3), p.261-273</ispartof><rights>2003 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-442ce51129d5ff3419dab52c02465a93a3943cd4147a70396c5c529b32b671943</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1135(03)00032-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14686322$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12695049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Melter, O</creatorcontrib><creatorcontrib>Hercı́k, K</creatorcontrib><creatorcontrib>Weyant, R.S</creatorcontrib><creatorcontrib>Janeček, J</creatorcontrib><creatorcontrib>Nemec, A</creatorcontrib><creatorcontrib>Mecera, J</creatorcontrib><creatorcontrib>Gonzorová, L’</creatorcontrib><creatorcontrib>Branny, P</creatorcontrib><title>Detection and characterization of feline Bartonella henselae in the Czech Republic</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The aims of the study were to characterize isolates of
Bartonella henselae and to determine the prevalence of bacteremic domestic cats in urban and suburban parts of Prague, Czech Republic. Five (18%) gram-negative fastidious bacterial single-cat isolates were recovered from 27 hemocultures incubated without previous freezing. Four of these isolates originated from flea infested stray cats (
n=6) and one from a shelter cat without any ectoparasites (
n=21). None of the 34 previously frozen specimens from flea free pet cats yielded any bacteria. All five isolates were catalase and oxidase negative. Their enzymatic activity, RFLP profile of citrate synthetase gene (
gltA) and DNA–DNA hybridization results were typical of
B. henselae. According to their
PvuII and
BglI ribotypes the isolates could be allocated to two homogeneous groups. Ribotype
HindIII and RFLP of 16S–23S rRNA spacer region analysis gave unique profiles different from those of
Bartonella quintana,
Bartonella elizabethae and
Bartonella clarridgeiae. The 16S rRNA type-specific amplification revealed an identical profile typical of
B. henselae genotype II for all the cat isolates studied. Pulsed-field gel electrophoresis (PFGE) assigned a different profile to each of the isolates studied.
Determination of the enzymatic activity, RFLP of
gltA gene, RFLP of 16S–23S rRNA spacer region, and
HindIII ribotype could be efficient tools for identification of
B. henselae isolates. Ribotyping (
PvuII,
BglI), 16S rRNA typing and PFGE may be useful methods to prospect ecology and epidemiology of the agent.</description><subject>Animals</subject><subject>bacteria</subject><subject>Bacteriology</subject><subject>Bartonella clarridgeiae</subject><subject>Bartonella elizabethae</subject><subject>Bartonella henselae</subject><subject>Bartonella henselae - enzymology</subject><subject>Bartonella henselae - genetics</subject><subject>Bartonella henselae - isolation & purification</subject><subject>Bartonella Infections - enzymology</subject><subject>Bartonella Infections - epidemiology</subject><subject>Bartonella Infections - microbiology</subject><subject>Bartonella Infections - veterinary</subject><subject>Bartonella quintana</subject><subject>Biological and medical sciences</subject><subject>Cat</subject><subject>Cat Diseases - epidemiology</subject><subject>Cat Diseases - microbiology</subject><subject>catalase</subject><subject>Catalase - metabolism</subject><subject>Cats</subject><subject>Citrate (si)-Synthase - chemistry</subject><subject>Citrate (si)-Synthase - genetics</subject><subject>citrates</subject><subject>Czech Republic</subject><subject>Czech Republic - epidemiology</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal Spacer - chemistry</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>ectoparasites</subject><subject>Electrophoresis, Gel, Pulsed-Field - veterinary</subject><subject>enzyme activity</subject><subject>Epidemiology</subject><subject>Female</subject><subject>freezing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>hybridization</subject><subject>Male</subject><subject>Microbiology</subject><subject>Molecular typing</subject><subject>Nucleic Acid Hybridization</subject><subject>Oxidoreductases - metabolism</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>pulsed-field gel electrophoresis</subject><subject>ribosomal RNA</subject><subject>ribotypes</subject><subject>Ribotyping - veterinary</subject><subject>RNA, Ribosomal, 16S - chemistry</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Siphonaptera</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UuPFCEQB3BiNO64-hFULho9tPLu4WR0fCabmOy6Z1JdXe1geugRekzcTy_ziHvcEwF-UMUfxp5K8UYK6d5eCd0uGym1fSX0ayGEVo25xxZy2epGWaPus8V_csYelfKrIuOdeMjOpHLe1smCXX6kmXCOU-KQeo5ryIAz5XgDh8Vp4AONMRH_AHmeEo0j8DWlQiMQj4nPa-KrG8I1v6TtrhsjPmYPBhgLPTmN5-z686cfq6_Nxfcv31bvLxq0Us2NMQrJSql8b4dBG-l76KxCoYyz4DVobzT2RpoWWqG9Q4tW-U6rzrWy7p2zl8d7t3n6vaMyh00suG8w0bQrodXS-_rMO2GNTJiKK7RHiHkqJdMQtjluIP8NUoR96uGQethHGoQOh9TDvpNnpwK7bkP97alTzBW8OAEoCOOQIWEst864pdNKVff86AaYAvzM1VxfKSFNrdQurXFVvDsKqsn-iZRDwUgJqY-5_mPop3hHs_8A79Slqw</recordid><startdate>20030529</startdate><enddate>20030529</enddate><creator>Melter, O</creator><creator>Hercı́k, K</creator><creator>Weyant, R.S</creator><creator>Janeček, J</creator><creator>Nemec, A</creator><creator>Mecera, J</creator><creator>Gonzorová, L’</creator><creator>Branny, P</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20030529</creationdate><title>Detection and characterization of feline Bartonella henselae in the Czech Republic</title><author>Melter, O ; Hercı́k, K ; Weyant, R.S ; Janeček, J ; Nemec, A ; Mecera, J ; Gonzorová, L’ ; Branny, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-442ce51129d5ff3419dab52c02465a93a3943cd4147a70396c5c529b32b671943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>bacteria</topic><topic>Bacteriology</topic><topic>Bartonella clarridgeiae</topic><topic>Bartonella elizabethae</topic><topic>Bartonella henselae</topic><topic>Bartonella henselae - enzymology</topic><topic>Bartonella henselae - genetics</topic><topic>Bartonella henselae - isolation & purification</topic><topic>Bartonella Infections - enzymology</topic><topic>Bartonella Infections - epidemiology</topic><topic>Bartonella Infections - microbiology</topic><topic>Bartonella Infections - veterinary</topic><topic>Bartonella quintana</topic><topic>Biological and medical sciences</topic><topic>Cat</topic><topic>Cat Diseases - epidemiology</topic><topic>Cat Diseases - microbiology</topic><topic>catalase</topic><topic>Catalase - metabolism</topic><topic>Cats</topic><topic>Citrate (si)-Synthase - chemistry</topic><topic>Citrate (si)-Synthase - genetics</topic><topic>citrates</topic><topic>Czech Republic</topic><topic>Czech Republic - epidemiology</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal Spacer - chemistry</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>ectoparasites</topic><topic>Electrophoresis, Gel, Pulsed-Field - veterinary</topic><topic>enzyme activity</topic><topic>Epidemiology</topic><topic>Female</topic><topic>freezing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>hybridization</topic><topic>Male</topic><topic>Microbiology</topic><topic>Molecular typing</topic><topic>Nucleic Acid Hybridization</topic><topic>Oxidoreductases - metabolism</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>pulsed-field gel electrophoresis</topic><topic>ribosomal RNA</topic><topic>ribotypes</topic><topic>Ribotyping - veterinary</topic><topic>RNA, Ribosomal, 16S - chemistry</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Siphonaptera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Melter, O</creatorcontrib><creatorcontrib>Hercı́k, K</creatorcontrib><creatorcontrib>Weyant, R.S</creatorcontrib><creatorcontrib>Janeček, J</creatorcontrib><creatorcontrib>Nemec, A</creatorcontrib><creatorcontrib>Mecera, J</creatorcontrib><creatorcontrib>Gonzorová, L’</creatorcontrib><creatorcontrib>Branny, P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Melter, O</au><au>Hercı́k, K</au><au>Weyant, R.S</au><au>Janeček, J</au><au>Nemec, A</au><au>Mecera, J</au><au>Gonzorová, L’</au><au>Branny, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and characterization of feline Bartonella henselae in the Czech Republic</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2003-05-29</date><risdate>2003</risdate><volume>93</volume><issue>3</issue><spage>261</spage><epage>273</epage><pages>261-273</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The aims of the study were to characterize isolates of
Bartonella henselae and to determine the prevalence of bacteremic domestic cats in urban and suburban parts of Prague, Czech Republic. Five (18%) gram-negative fastidious bacterial single-cat isolates were recovered from 27 hemocultures incubated without previous freezing. Four of these isolates originated from flea infested stray cats (
n=6) and one from a shelter cat without any ectoparasites (
n=21). None of the 34 previously frozen specimens from flea free pet cats yielded any bacteria. All five isolates were catalase and oxidase negative. Their enzymatic activity, RFLP profile of citrate synthetase gene (
gltA) and DNA–DNA hybridization results were typical of
B. henselae. According to their
PvuII and
BglI ribotypes the isolates could be allocated to two homogeneous groups. Ribotype
HindIII and RFLP of 16S–23S rRNA spacer region analysis gave unique profiles different from those of
Bartonella quintana,
Bartonella elizabethae and
Bartonella clarridgeiae. The 16S rRNA type-specific amplification revealed an identical profile typical of
B. henselae genotype II for all the cat isolates studied. Pulsed-field gel electrophoresis (PFGE) assigned a different profile to each of the isolates studied.
Determination of the enzymatic activity, RFLP of
gltA gene, RFLP of 16S–23S rRNA spacer region, and
HindIII ribotype could be efficient tools for identification of
B. henselae isolates. Ribotyping (
PvuII,
BglI), 16S rRNA typing and PFGE may be useful methods to prospect ecology and epidemiology of the agent.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12695049</pmid><doi>10.1016/S0378-1135(03)00032-4</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 2003-05, Vol.93 (3), p.261-273 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73199950 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals bacteria Bacteriology Bartonella clarridgeiae Bartonella elizabethae Bartonella henselae Bartonella henselae - enzymology Bartonella henselae - genetics Bartonella henselae - isolation & purification Bartonella Infections - enzymology Bartonella Infections - epidemiology Bartonella Infections - microbiology Bartonella Infections - veterinary Bartonella quintana Biological and medical sciences Cat Cat Diseases - epidemiology Cat Diseases - microbiology catalase Catalase - metabolism Cats Citrate (si)-Synthase - chemistry Citrate (si)-Synthase - genetics citrates Czech Republic Czech Republic - epidemiology DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal Spacer - chemistry DNA, Ribosomal Spacer - genetics ectoparasites Electrophoresis, Gel, Pulsed-Field - veterinary enzyme activity Epidemiology Female freezing Fundamental and applied biological sciences. Psychology genes hybridization Male Microbiology Molecular typing Nucleic Acid Hybridization Oxidoreductases - metabolism Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length pulsed-field gel electrophoresis ribosomal RNA ribotypes Ribotyping - veterinary RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics Siphonaptera |
| title | Detection and characterization of feline Bartonella henselae in the Czech Republic |
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