Characterization by cDNA cloning of the mRNA of a highly basic human protein homologous to the yeast ribosomal protein YL41
From a cDNA library in λgt11 derived from poly(A) + mRNA of human ovarian granulosa cells, a cDNA clone λHG12.1, containing an EcoRI insert of 470 bp, was identified. After subcloning of the insert into pUC18, the clone pHG12.1 was obtained and sequenced. The 5′-region of the insert of pHG12.1 was e...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1992-09, Vol.187 (2), p.901-906 |
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| creator | Klaudiny, J. von der Kammer, H. Scheit, K.H. |
| description | From a cDNA library in λgt11 derived from poly(A)
+ mRNA of human ovarian granulosa cells, a cDNA clone λHG12.1, containing an
EcoRI insert of 470 bp, was identified. After subcloning of the insert into pUC18, the clone pHG12.1 was obtained and sequenced. The 5′-region of the insert of pHG12.1 was extended by the polymerase chain reaction (PCR) with cloned total cDNA. Assembly of the PCR fragment with the insert of pHG12.1 yielded clone pHG12. From the first open reading frame of pHG12 the amino acid sequence for a polypeptide of 25 amino acid residues (designated HG12) was derived, which was identical in 22 residues with yeast ribosomal protein YL41. It is therefore assumed that HG12 is the first mammalian homolog of yeast ribosomal protein YL41. Transcription of DNA fragments containing the coding region of pHG12 cloned into BluescriptM13, followed by cell-free translation, yielded a polypeptide with an apparent mol.wt. of 14.5 kDa, much larger than the theoretical mol.wt. (3454 Da). The discrepancy between theoretical and apparent mol.wt. was also observed for yeast ribosomal protein YL41. Southern analysis revealed that HG12 is not specified by a single copy gene. Homology for HG12 specific sequences is observed for bovine, porcine and rat species. |
| doi_str_mv | 10.1016/0006-291X(92)91282-U |
| format | Article |
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EcoRI insert of 470 bp, was identified. After subcloning of the insert into pUC18, the clone pHG12.1 was obtained and sequenced. The 5′-region of the insert of pHG12.1 was extended by the polymerase chain reaction (PCR) with cloned total cDNA. Assembly of the PCR fragment with the insert of pHG12.1 yielded clone pHG12. From the first open reading frame of pHG12 the amino acid sequence for a polypeptide of 25 amino acid residues (designated HG12) was derived, which was identical in 22 residues with yeast ribosomal protein YL41. It is therefore assumed that HG12 is the first mammalian homolog of yeast ribosomal protein YL41. Transcription of DNA fragments containing the coding region of pHG12 cloned into BluescriptM13, followed by cell-free translation, yielded a polypeptide with an apparent mol.wt. of 14.5 kDa, much larger than the theoretical mol.wt. (3454 Da). The discrepancy between theoretical and apparent mol.wt. was also observed for yeast ribosomal protein YL41. Southern analysis revealed that HG12 is not specified by a single copy gene. Homology for HG12 specific sequences is observed for bovine, porcine and rat species.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(92)91282-U</identifier><identifier>PMID: 1326959</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; cDNA ; Cloning, Molecular ; DNA - chemistry ; DNA - genetics ; DNA Restriction Enzymes ; Female ; Fundamental and applied biological sciences. Psychology ; genes ; Granulosa Cells - chemistry ; homology ; Humans ; man ; Miscellaneous ; Molecular Sequence Data ; nucleotide sequence ; Polymerase Chain Reaction ; predictions ; Proteins ; Proteins - chemistry ; Proteins - genetics ; ribosomal protein YL41 ; ribosomal proteins ; Ribosomal Proteins - chemistry ; Ribosomal Proteins - genetics ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Saccharomyces cerevisiae Proteins ; Sequence Homology, Nucleic Acid ; Swine</subject><ispartof>Biochemical and biophysical research communications, 1992-09, Vol.187 (2), p.901-906</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-b46fa5d2721df1268511cbeb75342cdd60863815af955c28288e11c5707aabda3</citedby><cites>FETCH-LOGICAL-c383t-b46fa5d2721df1268511cbeb75342cdd60863815af955c28288e11c5707aabda3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(92)91282-U$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5572871$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1326959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klaudiny, J.</creatorcontrib><creatorcontrib>von der Kammer, H.</creatorcontrib><creatorcontrib>Scheit, K.H.</creatorcontrib><title>Characterization by cDNA cloning of the mRNA of a highly basic human protein homologous to the yeast ribosomal protein YL41</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>From a cDNA library in λgt11 derived from poly(A)
+ mRNA of human ovarian granulosa cells, a cDNA clone λHG12.1, containing an
EcoRI insert of 470 bp, was identified. After subcloning of the insert into pUC18, the clone pHG12.1 was obtained and sequenced. The 5′-region of the insert of pHG12.1 was extended by the polymerase chain reaction (PCR) with cloned total cDNA. Assembly of the PCR fragment with the insert of pHG12.1 yielded clone pHG12. From the first open reading frame of pHG12 the amino acid sequence for a polypeptide of 25 amino acid residues (designated HG12) was derived, which was identical in 22 residues with yeast ribosomal protein YL41. It is therefore assumed that HG12 is the first mammalian homolog of yeast ribosomal protein YL41. Transcription of DNA fragments containing the coding region of pHG12 cloned into BluescriptM13, followed by cell-free translation, yielded a polypeptide with an apparent mol.wt. of 14.5 kDa, much larger than the theoretical mol.wt. (3454 Da). The discrepancy between theoretical and apparent mol.wt. was also observed for yeast ribosomal protein YL41. Southern analysis revealed that HG12 is not specified by a single copy gene. Homology for HG12 specific sequences is observed for bovine, porcine and rat species.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>cDNA</subject><subject>Cloning, Molecular</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA Restriction Enzymes</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Granulosa Cells - chemistry</subject><subject>homology</subject><subject>Humans</subject><subject>man</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>Polymerase Chain Reaction</subject><subject>predictions</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>Proteins - genetics</subject><subject>ribosomal protein YL41</subject><subject>ribosomal proteins</subject><subject>Ribosomal Proteins - chemistry</subject><subject>Ribosomal Proteins - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Swine</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoO4rOPqP1DIQUQPral05-siLOPHCoOCOKCnkE6npyPdnd0kI4z7583sDOPNPVWoet5K8b4IPQPyBgjwt4QQXlEFP14p-loBlbRaP0ALIIpUFEjzEC1OyCP0OKVfhAA0XJ2jc6gpV0wt0O1yMNHY7KL_Y7IPM2532L7_contGGY_b3DocR4cnr6VXnkbPPjNMO5wa5K3eNhOZsbXMWTnZzyEKYxhE7YJ53An2zmTMo6-DSlMZjyRP1cNPEFnvRmTe3qsF2j98cP35VW1-vrp8_JyVdla1rlqG94b1lFBoeuBcskAbOtaweqG2q7jRPJaAjO9YswWG6R0hWCCCGPaztQX6OVhb_n8ZutS1pNP1o2jmV05VYsalJRC3AsCrxvSCF7A5gDaGFKKrtfX0U8m7jQQvQ9H753Xe-e1ovouHL0usufH_dt2ct0_0SGNMn9xnJtkzdhHM1ufThhjgkoBBXt3wFwx7bd3USfr3Wxd56OzWXfB__-Ov3Csq00</recordid><startdate>19920916</startdate><enddate>19920916</enddate><creator>Klaudiny, J.</creator><creator>von der Kammer, H.</creator><creator>Scheit, K.H.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T3</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19920916</creationdate><title>Characterization by cDNA cloning of the mRNA of a highly basic human protein homologous to the yeast ribosomal protein YL41</title><author>Klaudiny, J. ; von der Kammer, H. ; Scheit, K.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-b46fa5d2721df1268511cbeb75342cdd60863815af955c28288e11c5707aabda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>cDNA</topic><topic>Cloning, Molecular</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA Restriction Enzymes</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Granulosa Cells - chemistry</topic><topic>homology</topic><topic>Humans</topic><topic>man</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>Polymerase Chain Reaction</topic><topic>predictions</topic><topic>Proteins</topic><topic>Proteins - chemistry</topic><topic>Proteins - genetics</topic><topic>ribosomal protein YL41</topic><topic>ribosomal proteins</topic><topic>Ribosomal Proteins - chemistry</topic><topic>Ribosomal Proteins - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klaudiny, J.</creatorcontrib><creatorcontrib>von der Kammer, H.</creatorcontrib><creatorcontrib>Scheit, K.H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klaudiny, J.</au><au>von der Kammer, H.</au><au>Scheit, K.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization by cDNA cloning of the mRNA of a highly basic human protein homologous to the yeast ribosomal protein YL41</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1992-09-16</date><risdate>1992</risdate><volume>187</volume><issue>2</issue><spage>901</spage><epage>906</epage><pages>901-906</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>From a cDNA library in λgt11 derived from poly(A)
+ mRNA of human ovarian granulosa cells, a cDNA clone λHG12.1, containing an
EcoRI insert of 470 bp, was identified. After subcloning of the insert into pUC18, the clone pHG12.1 was obtained and sequenced. The 5′-region of the insert of pHG12.1 was extended by the polymerase chain reaction (PCR) with cloned total cDNA. Assembly of the PCR fragment with the insert of pHG12.1 yielded clone pHG12. From the first open reading frame of pHG12 the amino acid sequence for a polypeptide of 25 amino acid residues (designated HG12) was derived, which was identical in 22 residues with yeast ribosomal protein YL41. It is therefore assumed that HG12 is the first mammalian homolog of yeast ribosomal protein YL41. Transcription of DNA fragments containing the coding region of pHG12 cloned into BluescriptM13, followed by cell-free translation, yielded a polypeptide with an apparent mol.wt. of 14.5 kDa, much larger than the theoretical mol.wt. (3454 Da). The discrepancy between theoretical and apparent mol.wt. was also observed for yeast ribosomal protein YL41. Southern analysis revealed that HG12 is not specified by a single copy gene. Homology for HG12 specific sequences is observed for bovine, porcine and rat species.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1326959</pmid><doi>10.1016/0006-291X(92)91282-U</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1992-09, Vol.187 (2), p.901-906 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Base Sequence Biological and medical sciences Blotting, Northern cDNA Cloning, Molecular DNA - chemistry DNA - genetics DNA Restriction Enzymes Female Fundamental and applied biological sciences. Psychology genes Granulosa Cells - chemistry homology Humans man Miscellaneous Molecular Sequence Data nucleotide sequence Polymerase Chain Reaction predictions Proteins Proteins - chemistry Proteins - genetics ribosomal protein YL41 ribosomal proteins Ribosomal Proteins - chemistry Ribosomal Proteins - genetics RNA, Messenger - analysis RNA, Messenger - genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Swine |
| title | Characterization by cDNA cloning of the mRNA of a highly basic human protein homologous to the yeast ribosomal protein YL41 |
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