Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I
We examined the distribution of calpains I and II in human hematopoietic system cell lines by Western and Northern blot analyses and enzyme activity assay. Expression of calpain I, a low Ca(2+)-requiring cysteine protease, was observed in all human T-cell lines tested. By contrast, expression of cal...
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| Veröffentlicht in: | The Journal of biological chemistry 1992-09, Vol.267 (27), p.19373-19378 |
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| container_title | The Journal of biological chemistry |
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| creator | ADACHI, Y KITAHARA-OZAWA, A SUGAMURA, K WOON-JOO LEE YODOI, J MAKI, M MURACHI, T HATANAKA, M |
| description | We examined the distribution of calpains I and II in human hematopoietic system cell lines by Western and Northern blot analyses
and enzyme activity assay. Expression of calpain I, a low Ca(2+)-requiring cysteine protease, was observed in all human T-cell
lines tested. By contrast, expression of calpain II, a high Ca(2+)-requiring form, in human T-cells was closely correlated
with human T-cell leukemia virus type I (HTLV-I) infection, which is known to result in the expression of adult T-cell leukemia-associated
antigens, interleukin-2 (IL-2) receptor alpha, and Ca(2+)-dependent cell proliferation. Specific expression of calpain II
in HTLV-I-infected cells occurred at the mRNA level. Furthermore, expression of calpain II in human natural killer-like cells
was augmented by HTLV-I pX gene transfection. In HTLV-I-infected cells, the trans-acting transcriptional activation of the
long terminal repeat and control elements for the IL-2 receptor alpha, c-fos, and granulocyte-macrophage colony-stimulating
factor genes by the Tax from the pX gene is already known. Our results suggest that the similar trans-activation occurs to
the calpain II gene in HTLV-I-infected hematopoietic system cells. |
| doi_str_mv | 10.1016/S0021-9258(18)41785-1 |
| format | Article |
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and enzyme activity assay. Expression of calpain I, a low Ca(2+)-requiring cysteine protease, was observed in all human T-cell
lines tested. By contrast, expression of calpain II, a high Ca(2+)-requiring form, in human T-cells was closely correlated
with human T-cell leukemia virus type I (HTLV-I) infection, which is known to result in the expression of adult T-cell leukemia-associated
antigens, interleukin-2 (IL-2) receptor alpha, and Ca(2+)-dependent cell proliferation. Specific expression of calpain II
in HTLV-I-infected cells occurred at the mRNA level. Furthermore, expression of calpain II in human natural killer-like cells
was augmented by HTLV-I pX gene transfection. In HTLV-I-infected cells, the trans-acting transcriptional activation of the
long terminal repeat and control elements for the IL-2 receptor alpha, c-fos, and granulocyte-macrophage colony-stimulating
factor genes by the Tax from the pX gene is already known. Our results suggest that the similar trans-activation occurs to
the calpain II gene in HTLV-I-infected hematopoietic system cells.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)41785-1</identifier><identifier>PMID: 1527057</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>AIDS/HIV ; Biological and medical sciences ; Blotting, Western ; Calcium-Binding Proteins - metabolism ; Calpain - genetics ; Calpain - metabolism ; calpain II ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genes, pX ; hematopoiesis ; Hematopoietic Stem Cells - microbiology ; HTLV-I Infections - genetics ; Humans ; In Vitro Techniques ; infection ; lymphocytes T ; man ; Molecular and cellular biology ; Molecular genetics ; RNA, Messenger - genetics ; T cell leukemia virus I ; T-Lymphocytes - physiology ; Transfection ; Tumor Cells, Cultured</subject><ispartof>The Journal of biological chemistry, 1992-09, Vol.267 (27), p.19373-19378</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-1be69fde9c15c7657d67d5f7426bdf91548a32ce304828b1a96f47e29304e72b3</citedby><cites>FETCH-LOGICAL-c440t-1be69fde9c15c7657d67d5f7426bdf91548a32ce304828b1a96f47e29304e72b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4332082$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1527057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ADACHI, Y</creatorcontrib><creatorcontrib>KITAHARA-OZAWA, A</creatorcontrib><creatorcontrib>SUGAMURA, K</creatorcontrib><creatorcontrib>WOON-JOO LEE</creatorcontrib><creatorcontrib>YODOI, J</creatorcontrib><creatorcontrib>MAKI, M</creatorcontrib><creatorcontrib>MURACHI, T</creatorcontrib><creatorcontrib>HATANAKA, M</creatorcontrib><title>Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>We examined the distribution of calpains I and II in human hematopoietic system cell lines by Western and Northern blot analyses
and enzyme activity assay. Expression of calpain I, a low Ca(2+)-requiring cysteine protease, was observed in all human T-cell
lines tested. By contrast, expression of calpain II, a high Ca(2+)-requiring form, in human T-cells was closely correlated
with human T-cell leukemia virus type I (HTLV-I) infection, which is known to result in the expression of adult T-cell leukemia-associated
antigens, interleukin-2 (IL-2) receptor alpha, and Ca(2+)-dependent cell proliferation. Specific expression of calpain II
in HTLV-I-infected cells occurred at the mRNA level. Furthermore, expression of calpain II in human natural killer-like cells
was augmented by HTLV-I pX gene transfection. In HTLV-I-infected cells, the trans-acting transcriptional activation of the
long terminal repeat and control elements for the IL-2 receptor alpha, c-fos, and granulocyte-macrophage colony-stimulating
factor genes by the Tax from the pX gene is already known. Our results suggest that the similar trans-activation occurs to
the calpain II gene in HTLV-I-infected hematopoietic system cells.</description><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Calpain - genetics</subject><subject>Calpain - metabolism</subject><subject>calpain II</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes, pX</subject><subject>hematopoiesis</subject><subject>Hematopoietic Stem Cells - microbiology</subject><subject>HTLV-I Infections - genetics</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>infection</subject><subject>lymphocytes T</subject><subject>man</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>RNA, Messenger - genetics</subject><subject>T cell leukemia virus I</subject><subject>T-Lymphocytes - physiology</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAURi0EKkPhJ1TyAiFYhPr6GS9R1cJIlVjQSuwsx7npGPLCTmjn35N0Ru0SL2zL37nXlg8hZ8A-AwN9_oMxDoXlqvwI5ScJplQFvCAbYKUohIKfL8nmCXlN3uT8iy1DWjghJ6C4YcpsSLp8GBPmHIeeDg0Nvh197Ol2S--wR7psd3Pnlxk7Pw3jEHGKgeZ9nrCjAds2L0yDYcKa3sdpd8RvijWjLc6_sYue_o1pznTaj0i3b8mrxrcZ3x3XU3J7dXlz8a24_v51e_HlughSsqmACrVtarQBVDBamVqbWjVGcl3VjQUlSy94QMFkycsKvNWNNMjtcoCGV-KUfDj0HdPwZ8Y8uS7m9Vm-x2HOzgiwWmr5XxC0kELaFVQHMKQh54SNG1PsfNo7YG6V4h6luPXHHZTuUYqDpe7seMFcdVg_Vx0sLPn7Y-7zYqBJvg8xP2FSCM5K_ozt4t3uPiZ0VRzCYsZxbRw3DqwwQvwDpBWgaQ</recordid><startdate>19920925</startdate><enddate>19920925</enddate><creator>ADACHI, Y</creator><creator>KITAHARA-OZAWA, A</creator><creator>SUGAMURA, K</creator><creator>WOON-JOO LEE</creator><creator>YODOI, J</creator><creator>MAKI, M</creator><creator>MURACHI, T</creator><creator>HATANAKA, M</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19920925</creationdate><title>Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I</title><author>ADACHI, Y ; KITAHARA-OZAWA, A ; SUGAMURA, K ; WOON-JOO LEE ; YODOI, J ; MAKI, M ; MURACHI, T ; HATANAKA, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-1be69fde9c15c7657d67d5f7426bdf91548a32ce304828b1a96f47e29304e72b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Calpain - genetics</topic><topic>Calpain - metabolism</topic><topic>calpain II</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes, pX</topic><topic>hematopoiesis</topic><topic>Hematopoietic Stem Cells - microbiology</topic><topic>HTLV-I Infections - genetics</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>infection</topic><topic>lymphocytes T</topic><topic>man</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>RNA, Messenger - genetics</topic><topic>T cell leukemia virus I</topic><topic>T-Lymphocytes - physiology</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ADACHI, Y</creatorcontrib><creatorcontrib>KITAHARA-OZAWA, A</creatorcontrib><creatorcontrib>SUGAMURA, K</creatorcontrib><creatorcontrib>WOON-JOO LEE</creatorcontrib><creatorcontrib>YODOI, J</creatorcontrib><creatorcontrib>MAKI, M</creatorcontrib><creatorcontrib>MURACHI, T</creatorcontrib><creatorcontrib>HATANAKA, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ADACHI, Y</au><au>KITAHARA-OZAWA, A</au><au>SUGAMURA, K</au><au>WOON-JOO LEE</au><au>YODOI, J</au><au>MAKI, M</au><au>MURACHI, T</au><au>HATANAKA, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1992-09-25</date><risdate>1992</risdate><volume>267</volume><issue>27</issue><spage>19373</spage><epage>19378</epage><pages>19373-19378</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>We examined the distribution of calpains I and II in human hematopoietic system cell lines by Western and Northern blot analyses
and enzyme activity assay. Expression of calpain I, a low Ca(2+)-requiring cysteine protease, was observed in all human T-cell
lines tested. By contrast, expression of calpain II, a high Ca(2+)-requiring form, in human T-cells was closely correlated
with human T-cell leukemia virus type I (HTLV-I) infection, which is known to result in the expression of adult T-cell leukemia-associated
antigens, interleukin-2 (IL-2) receptor alpha, and Ca(2+)-dependent cell proliferation. Specific expression of calpain II
in HTLV-I-infected cells occurred at the mRNA level. Furthermore, expression of calpain II in human natural killer-like cells
was augmented by HTLV-I pX gene transfection. In HTLV-I-infected cells, the trans-acting transcriptional activation of the
long terminal repeat and control elements for the IL-2 receptor alpha, c-fos, and granulocyte-macrophage colony-stimulating
factor genes by the Tax from the pX gene is already known. Our results suggest that the similar trans-activation occurs to
the calpain II gene in HTLV-I-infected hematopoietic system cells.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1527057</pmid><doi>10.1016/S0021-9258(18)41785-1</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1992-09, Vol.267 (27), p.19373-19378 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73196464 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | AIDS/HIV Biological and medical sciences Blotting, Western Calcium-Binding Proteins - metabolism Calpain - genetics Calpain - metabolism calpain II Fundamental and applied biological sciences. Psychology Gene Expression Genes, pX hematopoiesis Hematopoietic Stem Cells - microbiology HTLV-I Infections - genetics Humans In Vitro Techniques infection lymphocytes T man Molecular and cellular biology Molecular genetics RNA, Messenger - genetics T cell leukemia virus I T-Lymphocytes - physiology Transfection Tumor Cells, Cultured |
| title | Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I |
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