Failure of a recombinant Schistosoma bovis-derived glutathione S-transferase to protect cattle against experimental Fasciola hepatica infection
The potential of a recombinant Schistosoma bovis 28-kDa glutathione S-transferase (rSb28GST) to protect cattle against Fasciola hepatica was tested in a vaccination trial. Thirty two calves were randomly divided into four groups of eight animals. Calves of the three vaccine groups received two intra...
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| Veröffentlicht in: | Veterinary parasitology 2003-04, Vol.113 (2), p.135-144 |
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| creator | De Bont, J Claerebout, E Riveau, G Schacht, A.M Smets, K Conder, G Brake, D.A Capron, A Vercruysse, J |
| description | The potential of a recombinant
Schistosoma bovis 28-kDa glutathione S-transferase (rSb28GST) to protect cattle against
Fasciola hepatica was tested in a vaccination trial. Thirty two calves were randomly divided into four groups of eight animals. Calves of the three vaccine groups received two intramuscular injections at 3 weeks interval, of 0.250
mg rSb28GST in either aluminium hydroxide (Al(OH)
3), Quil A, or PBS emulsified in an equal volume of Freund’s complete adjuvant (FCA).
Animals of the control group received injections of Al(OH)
3/PBS only. All animals were challenged orally with a total of 360 metacercariae of
F. hepatica, spread over 6 weeks.
All groups of vaccinated animals produced measurable IgG antibody titers to rSb28GST after vaccination. Animals immunised with FCA adjuvanted vaccine had the highest and more durable antibody titers and only sera from this group recognised an approximately 24
kDa protein band from
F. hepatica, that is thought to be a
F. hepatica GST. Despite a good antibody response differences in cumulative faecal egg output between the groups were not statistically significant. In addition, no significant difference was found between groups in terms of total worm numbers or percentage of immature flukes recovered at necropsy. In conclusion, the recombinant
S. bovis 28
kDa GST was not found to adequately protect cattle against experimental
F. hepatica challenge, using either aluminium hydroxide, Quil A or FCA as adjuvant. |
| doi_str_mv | 10.1016/S0304-4017(02)00450-8 |
| format | Article |
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Schistosoma bovis 28-kDa glutathione S-transferase (rSb28GST) to protect cattle against
Fasciola hepatica was tested in a vaccination trial. Thirty two calves were randomly divided into four groups of eight animals. Calves of the three vaccine groups received two intramuscular injections at 3 weeks interval, of 0.250
mg rSb28GST in either aluminium hydroxide (Al(OH)
3), Quil A, or PBS emulsified in an equal volume of Freund’s complete adjuvant (FCA).
Animals of the control group received injections of Al(OH)
3/PBS only. All animals were challenged orally with a total of 360 metacercariae of
F. hepatica, spread over 6 weeks.
All groups of vaccinated animals produced measurable IgG antibody titers to rSb28GST after vaccination. Animals immunised with FCA adjuvanted vaccine had the highest and more durable antibody titers and only sera from this group recognised an approximately 24
kDa protein band from
F. hepatica, that is thought to be a
F. hepatica GST. Despite a good antibody response differences in cumulative faecal egg output between the groups were not statistically significant. In addition, no significant difference was found between groups in terms of total worm numbers or percentage of immature flukes recovered at necropsy. In conclusion, the recombinant
S. bovis 28
kDa GST was not found to adequately protect cattle against experimental
F. hepatica challenge, using either aluminium hydroxide, Quil A or FCA as adjuvant.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/S0304-4017(02)00450-8</identifier><identifier>PMID: 12695038</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adjuvants, Immunologic - pharmacology ; Animals ; Antibodies, Helminth - blood ; Cattle ; Cattle Diseases - immunology ; Cattle Diseases - parasitology ; Cattle Diseases - prevention & control ; Cattle-trematoda ; Eosinophilia - immunology ; Fasciola hepatica ; Fasciola hepatica - growth & development ; Fasciola hepatica - immunology ; Fascioliasis - immunology ; Fascioliasis - parasitology ; Fascioliasis - prevention & control ; Fascioliasis - veterinary ; Feces - parasitology ; Female ; gamma-Glutamyltransferase - blood ; Glutathione S-transferase ; Glutathione Transferase - immunology ; Glutathione Transferase - pharmacology ; Immunization - veterinary ; Parasite Egg Count - veterinary ; Random Allocation ; Recombinant Proteins - immunology ; Recombinant Proteins - pharmacology ; Schistosoma - enzymology ; Schistosoma - immunology ; Vaccine</subject><ispartof>Veterinary parasitology, 2003-04, Vol.113 (2), p.135-144</ispartof><rights>2003 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-d23556b2818314d20dd1c0e8f566715df9e23eae1d15f0b81e672ad09faf5d343</citedby><cites>FETCH-LOGICAL-c408t-d23556b2818314d20dd1c0e8f566715df9e23eae1d15f0b81e672ad09faf5d343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-4017(02)00450-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12695038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Bont, J</creatorcontrib><creatorcontrib>Claerebout, E</creatorcontrib><creatorcontrib>Riveau, G</creatorcontrib><creatorcontrib>Schacht, A.M</creatorcontrib><creatorcontrib>Smets, K</creatorcontrib><creatorcontrib>Conder, G</creatorcontrib><creatorcontrib>Brake, D.A</creatorcontrib><creatorcontrib>Capron, A</creatorcontrib><creatorcontrib>Vercruysse, J</creatorcontrib><title>Failure of a recombinant Schistosoma bovis-derived glutathione S-transferase to protect cattle against experimental Fasciola hepatica infection</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>The potential of a recombinant
Schistosoma bovis 28-kDa glutathione S-transferase (rSb28GST) to protect cattle against
Fasciola hepatica was tested in a vaccination trial. Thirty two calves were randomly divided into four groups of eight animals. Calves of the three vaccine groups received two intramuscular injections at 3 weeks interval, of 0.250
mg rSb28GST in either aluminium hydroxide (Al(OH)
3), Quil A, or PBS emulsified in an equal volume of Freund’s complete adjuvant (FCA).
Animals of the control group received injections of Al(OH)
3/PBS only. All animals were challenged orally with a total of 360 metacercariae of
F. hepatica, spread over 6 weeks.
All groups of vaccinated animals produced measurable IgG antibody titers to rSb28GST after vaccination. Animals immunised with FCA adjuvanted vaccine had the highest and more durable antibody titers and only sera from this group recognised an approximately 24
kDa protein band from
F. hepatica, that is thought to be a
F. hepatica GST. Despite a good antibody response differences in cumulative faecal egg output between the groups were not statistically significant. In addition, no significant difference was found between groups in terms of total worm numbers or percentage of immature flukes recovered at necropsy. In conclusion, the recombinant
S. bovis 28
kDa GST was not found to adequately protect cattle against experimental
F. hepatica challenge, using either aluminium hydroxide, Quil A or FCA as adjuvant.</description><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Cattle</subject><subject>Cattle Diseases - immunology</subject><subject>Cattle Diseases - parasitology</subject><subject>Cattle Diseases - prevention & control</subject><subject>Cattle-trematoda</subject><subject>Eosinophilia - immunology</subject><subject>Fasciola hepatica</subject><subject>Fasciola hepatica - growth & development</subject><subject>Fasciola hepatica - immunology</subject><subject>Fascioliasis - immunology</subject><subject>Fascioliasis - parasitology</subject><subject>Fascioliasis - prevention & control</subject><subject>Fascioliasis - veterinary</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>gamma-Glutamyltransferase - blood</subject><subject>Glutathione S-transferase</subject><subject>Glutathione Transferase - immunology</subject><subject>Glutathione Transferase - pharmacology</subject><subject>Immunization - veterinary</subject><subject>Parasite Egg Count - veterinary</subject><subject>Random Allocation</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Schistosoma - enzymology</subject><subject>Schistosoma - immunology</subject><subject>Vaccine</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EokvhJ4B8QuUQOk7iJHtCqOoWpEo9LJytiT3uGiX2Yjsr-BX8Zdzuqhw5zeV7b2beY-ytgI8CRHe5hQbaqgXRX0D9AaCVUA3P2EoMfVPVUsJztnpCztirlH5AoaDrX7IzUXdrCc2wYn826KYlEg-WI4-kwzw6jz7zrd65lEMKM_IxHFyqDEV3IMPvpyVj3rngiW-rHNEnSxET8Rz4PoZMOnONOU_E8R6dT5nTr31Rz-QzTnyDSbswId_RHrPTyJ23RVQcX7MXFqdEb07znH3fXH-7-lLd3t18vfp8W-kWhlyZupGyG-tBDI1oTQ3GCA00WNl1vZDGrqluCEkYIS2Mg6Cur9HA2qKVpmmbc_b-6Fvu_blQymp2SdM0oaewJNU3Yt1BDwWUR1DHkFIkq_blD4y_lQD10IR6bEI9xKygVo9NqKHo3p0WLONM5p_qFH0BPh0BKm8eHEVVQiGvybhSQ1YmuP-s-As4ppu4</recordid><startdate>20030418</startdate><enddate>20030418</enddate><creator>De Bont, J</creator><creator>Claerebout, E</creator><creator>Riveau, G</creator><creator>Schacht, A.M</creator><creator>Smets, K</creator><creator>Conder, G</creator><creator>Brake, D.A</creator><creator>Capron, A</creator><creator>Vercruysse, J</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030418</creationdate><title>Failure of a recombinant Schistosoma bovis-derived glutathione S-transferase to protect cattle against experimental Fasciola hepatica infection</title><author>De Bont, J ; Claerebout, E ; Riveau, G ; Schacht, A.M ; Smets, K ; Conder, G ; Brake, D.A ; Capron, A ; Vercruysse, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-d23556b2818314d20dd1c0e8f566715df9e23eae1d15f0b81e672ad09faf5d343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Cattle</topic><topic>Cattle Diseases - immunology</topic><topic>Cattle Diseases - parasitology</topic><topic>Cattle Diseases - prevention & control</topic><topic>Cattle-trematoda</topic><topic>Eosinophilia - immunology</topic><topic>Fasciola hepatica</topic><topic>Fasciola hepatica - growth & development</topic><topic>Fasciola hepatica - immunology</topic><topic>Fascioliasis - immunology</topic><topic>Fascioliasis - parasitology</topic><topic>Fascioliasis - prevention & control</topic><topic>Fascioliasis - veterinary</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>gamma-Glutamyltransferase - blood</topic><topic>Glutathione S-transferase</topic><topic>Glutathione Transferase - immunology</topic><topic>Glutathione Transferase - pharmacology</topic><topic>Immunization - veterinary</topic><topic>Parasite Egg Count - veterinary</topic><topic>Random Allocation</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Schistosoma - enzymology</topic><topic>Schistosoma - immunology</topic><topic>Vaccine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Bont, J</creatorcontrib><creatorcontrib>Claerebout, E</creatorcontrib><creatorcontrib>Riveau, G</creatorcontrib><creatorcontrib>Schacht, A.M</creatorcontrib><creatorcontrib>Smets, K</creatorcontrib><creatorcontrib>Conder, G</creatorcontrib><creatorcontrib>Brake, D.A</creatorcontrib><creatorcontrib>Capron, A</creatorcontrib><creatorcontrib>Vercruysse, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Bont, J</au><au>Claerebout, E</au><au>Riveau, G</au><au>Schacht, A.M</au><au>Smets, K</au><au>Conder, G</au><au>Brake, D.A</au><au>Capron, A</au><au>Vercruysse, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Failure of a recombinant Schistosoma bovis-derived glutathione S-transferase to protect cattle against experimental Fasciola hepatica infection</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2003-04-18</date><risdate>2003</risdate><volume>113</volume><issue>2</issue><spage>135</spage><epage>144</epage><pages>135-144</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>The potential of a recombinant
Schistosoma bovis 28-kDa glutathione S-transferase (rSb28GST) to protect cattle against
Fasciola hepatica was tested in a vaccination trial. Thirty two calves were randomly divided into four groups of eight animals. Calves of the three vaccine groups received two intramuscular injections at 3 weeks interval, of 0.250
mg rSb28GST in either aluminium hydroxide (Al(OH)
3), Quil A, or PBS emulsified in an equal volume of Freund’s complete adjuvant (FCA).
Animals of the control group received injections of Al(OH)
3/PBS only. All animals were challenged orally with a total of 360 metacercariae of
F. hepatica, spread over 6 weeks.
All groups of vaccinated animals produced measurable IgG antibody titers to rSb28GST after vaccination. Animals immunised with FCA adjuvanted vaccine had the highest and more durable antibody titers and only sera from this group recognised an approximately 24
kDa protein band from
F. hepatica, that is thought to be a
F. hepatica GST. Despite a good antibody response differences in cumulative faecal egg output between the groups were not statistically significant. In addition, no significant difference was found between groups in terms of total worm numbers or percentage of immature flukes recovered at necropsy. In conclusion, the recombinant
S. bovis 28
kDa GST was not found to adequately protect cattle against experimental
F. hepatica challenge, using either aluminium hydroxide, Quil A or FCA as adjuvant.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>12695038</pmid><doi>10.1016/S0304-4017(02)00450-8</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 2003-04, Vol.113 (2), p.135-144 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73196070 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adjuvants, Immunologic - pharmacology Animals Antibodies, Helminth - blood Cattle Cattle Diseases - immunology Cattle Diseases - parasitology Cattle Diseases - prevention & control Cattle-trematoda Eosinophilia - immunology Fasciola hepatica Fasciola hepatica - growth & development Fasciola hepatica - immunology Fascioliasis - immunology Fascioliasis - parasitology Fascioliasis - prevention & control Fascioliasis - veterinary Feces - parasitology Female gamma-Glutamyltransferase - blood Glutathione S-transferase Glutathione Transferase - immunology Glutathione Transferase - pharmacology Immunization - veterinary Parasite Egg Count - veterinary Random Allocation Recombinant Proteins - immunology Recombinant Proteins - pharmacology Schistosoma - enzymology Schistosoma - immunology Vaccine |
| title | Failure of a recombinant Schistosoma bovis-derived glutathione S-transferase to protect cattle against experimental Fasciola hepatica infection |
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