Amyloidogenic synthetic peptides of beta2-microglobulin--a role of the disulfide bond
To search for the essential regions responsible for the beta2-microglobulin (beta2-m) amyloid fibril formation, we synthesized six peptides corresponding to six of the seven beta-sheets in the native structure of beta2-m, and examined their amyloidogenicity. Among the peptides examined, peptide (21-...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2003-04, Vol.304 (1), p.101-106 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Hasegawa, Kazuhiro Ohhashi, Yumiko Yamaguchi, Itaru Takahashi, Naoki Tsutsumi, Shinobu Goto, Yuji Gejyo, Fumitake Naiki, Hironobu |
| description | To search for the essential regions responsible for the beta2-microglobulin (beta2-m) amyloid fibril formation, we synthesized six peptides corresponding to six of the seven beta-sheets in the native structure of beta2-m, and examined their amyloidogenicity. Among the peptides examined, peptide (21-31) (strand B) and the mixture of peptide (21-31) and (78-86) (strand F) showed fibril formation at both pH 2.5 and 7.5. Peptide (21-31) is the N-terminal half of the previously reported proteolytic fragment of beta2-m, Ser21-Lys41 (K3), suggesting that this region may be the essential core. Interestingly, the dimer formation of peptide (21-31) by the disulfide bond substantially facilitated the fibril formation, indicating that the disulfide bond is important for the structural stability of the fibrils. |
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Among the peptides examined, peptide (21-31) (strand B) and the mixture of peptide (21-31) and (78-86) (strand F) showed fibril formation at both pH 2.5 and 7.5. Peptide (21-31) is the N-terminal half of the previously reported proteolytic fragment of beta2-m, Ser21-Lys41 (K3), suggesting that this region may be the essential core. 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| ispartof | Biochemical and biophysical research communications, 2003-04, Vol.304 (1), p.101-106 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Amyloid - chemistry Amyloid - ultrastructure beta 2-Microglobulin - chemistry Chromatography, High Pressure Liquid Disulfides - chemistry Dithiothreitol - pharmacology Kinetics Molecular Sequence Data Peptides - chemistry Protein Structure, Secondary Sequence Deletion |
| title | Amyloidogenic synthetic peptides of beta2-microglobulin--a role of the disulfide bond |
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