In vivo transcriptional analysis of three baculovirus genes: Evidence of homology between viral and host transcripts
Transcripts for the gp64 and polyhedrin genes as well as a 1629 open reading frame (1629 ORF) of the Autographa californica nuclear polyhedrosis virus were examined in the midgut tissues and hemocytes of uninfected and infected host Trichoplusia ni larvae and Sf21 cells. Polyhedrin-specific transcri...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1992-09, Vol.190 (1), p.288-297 |
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| creator | Pham, Daphne Q.-D. Sivasubramanian, Nataraian |
| description | Transcripts for the gp64 and polyhedrin genes as well as a 1629 open reading frame (1629 ORF) of the
Autographa californica nuclear polyhedrosis virus were examined in the midgut tissues and hemocytes of uninfected and infected host
Trichoplusia ni larvae and Sf21 cells. Polyhedrin-specific transcripts of 1.2, 3.4, and 4.9 kb were expressed in both infected larval tissues and infected Sf21 cells. The highest level of expression for polyhedrin-specific transcripts was observed in hemocytes, whereas the lowest level occurred in midgut. The expression of the 2.0-kb transcript for the gp64 gene increased continuously through 72 hr postinfection in the infected midgut tissues. This transcript was also observed in infected hemocytes, though its expression declined at 72 hr postinfection, as the expression for polyhedrin-specific transcripts peaked. The 1629 ORF transcripts of 2.0 and 3.2 kb were expressed in both types of infected tissues. More significantly, a 1629-ORF-specific probe detected host transcripts of 0.7 and 2.5 kb in uninfected midgut tissues as well as transcripts of 2.5, 8.8, and 11.0 kb in uninfected hemocytes under high stringency conditions. The latter results indicate that these host transcripts share homology with the 1629 ORF gene. |
| doi_str_mv | 10.1016/0042-6822(92)91215-G |
| format | Article |
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Autographa californica nuclear polyhedrosis virus were examined in the midgut tissues and hemocytes of uninfected and infected host
Trichoplusia ni larvae and Sf21 cells. Polyhedrin-specific transcripts of 1.2, 3.4, and 4.9 kb were expressed in both infected larval tissues and infected Sf21 cells. The highest level of expression for polyhedrin-specific transcripts was observed in hemocytes, whereas the lowest level occurred in midgut. The expression of the 2.0-kb transcript for the gp64 gene increased continuously through 72 hr postinfection in the infected midgut tissues. This transcript was also observed in infected hemocytes, though its expression declined at 72 hr postinfection, as the expression for polyhedrin-specific transcripts peaked. The 1629 ORF transcripts of 2.0 and 3.2 kb were expressed in both types of infected tissues. More significantly, a 1629-ORF-specific probe detected host transcripts of 0.7 and 2.5 kb in uninfected midgut tissues as well as transcripts of 2.5, 8.8, and 11.0 kb in uninfected hemocytes under high stringency conditions. The latter results indicate that these host transcripts share homology with the 1629 ORF gene.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(92)91215-G</identifier><identifier>PMID: 1529534</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; ARN ; AUTOGRAPHA CALIFORNICA ; Bacteriology ; BACULOVIRIDAE ; Baculoviridae - genetics ; Biological and medical sciences ; Blotting, Northern ; Cell Line ; CELLULE SANGUINE ; CELULAS SANGUINEAS ; CICLO VITAL ; Cloning, Molecular ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; CYCLE DE DEVELOPPEMENT ; EXPRESION GENICA ; EXPRESSION DES GENES ; FENOTIPOS ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes, Viral ; Growth, nutrition, cell differenciation ; INFECCION ; INFECTION ; INTESTIN ; INTESTINOS ; LARVAS ; LARVE ; Microbiology ; Moths ; nuclear polyhedrosis virus ; Occlusion Body Matrix Proteins ; Open Reading Frames ; PHENOTYPE ; PROTEINAS ; PROTEINE ; Sequence Homology, Nucleic Acid ; SPODOPTERA FRUGIPERDA ; Transcription, Genetic ; TRICHOPLUSIA NI ; Viral Proteins - genetics ; Viral Proteins - metabolism ; Viral Structural Proteins ; VIRUS</subject><ispartof>Virology (New York, N.Y.), 1992-09, Vol.190 (1), p.288-297</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-cdf0f58266404a6db5567212c798833b27a744694a11a193be03d69cf6efb1fc3</citedby><cites>FETCH-LOGICAL-c351t-cdf0f58266404a6db5567212c798833b27a744694a11a193be03d69cf6efb1fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/004268229291215G$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4316314$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1529534$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pham, Daphne Q.-D.</creatorcontrib><creatorcontrib>Sivasubramanian, Nataraian</creatorcontrib><title>In vivo transcriptional analysis of three baculovirus genes: Evidence of homology between viral and host transcripts</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Transcripts for the gp64 and polyhedrin genes as well as a 1629 open reading frame (1629 ORF) of the
Autographa californica nuclear polyhedrosis virus were examined in the midgut tissues and hemocytes of uninfected and infected host
Trichoplusia ni larvae and Sf21 cells. Polyhedrin-specific transcripts of 1.2, 3.4, and 4.9 kb were expressed in both infected larval tissues and infected Sf21 cells. The highest level of expression for polyhedrin-specific transcripts was observed in hemocytes, whereas the lowest level occurred in midgut. The expression of the 2.0-kb transcript for the gp64 gene increased continuously through 72 hr postinfection in the infected midgut tissues. This transcript was also observed in infected hemocytes, though its expression declined at 72 hr postinfection, as the expression for polyhedrin-specific transcripts peaked. The 1629 ORF transcripts of 2.0 and 3.2 kb were expressed in both types of infected tissues. More significantly, a 1629-ORF-specific probe detected host transcripts of 0.7 and 2.5 kb in uninfected midgut tissues as well as transcripts of 2.5, 8.8, and 11.0 kb in uninfected hemocytes under high stringency conditions. The latter results indicate that these host transcripts share homology with the 1629 ORF gene.</description><subject>Animals</subject><subject>ARN</subject><subject>AUTOGRAPHA CALIFORNICA</subject><subject>Bacteriology</subject><subject>BACULOVIRIDAE</subject><subject>Baculoviridae - genetics</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cell Line</subject><subject>CELLULE SANGUINE</subject><subject>CELULAS SANGUINEAS</subject><subject>CICLO VITAL</subject><subject>Cloning, Molecular</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>CYCLE DE DEVELOPPEMENT</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FENOTIPOS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Viral</subject><subject>Growth, nutrition, cell differenciation</subject><subject>INFECCION</subject><subject>INFECTION</subject><subject>INTESTIN</subject><subject>INTESTINOS</subject><subject>LARVAS</subject><subject>LARVE</subject><subject>Microbiology</subject><subject>Moths</subject><subject>nuclear polyhedrosis virus</subject><subject>Occlusion Body Matrix Proteins</subject><subject>Open Reading Frames</subject><subject>PHENOTYPE</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>SPODOPTERA FRUGIPERDA</subject><subject>Transcription, Genetic</subject><subject>TRICHOPLUSIA NI</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><subject>Viral Structural Proteins</subject><subject>VIRUS</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVGL1DAUhYMo67j6B0ShDyL6UM1N0rTZB0GWdVxY8EH3OaTpzWyk04xJW5l_b2qH1SeFkBDOd0_COYS8APoOKMj3lApWyoaxN4q9VcCgKrcPyAaokiXlAh6SzT3ymDxJ6TvN97qmZ-QMKqYqLjZkvB6K2c-hGKMZko3-MPowmL4weTsmn4rgivEuIhatsVMfZh-nVOxwwHRRXM2-w8HiAt2FfejD7li0OP5EXGzjb58uS2n864H0lDxypk_47HSek9tPV98uP5c3X7bXlx9vSssrGEvbOeqqhkkpqDCya6tK1gyYrVXTcN6y2tRCSCUMgAHFW6S8k8o6ia4FZ_k5eb36HmL4MWEa9d4ni31vBgxT0jUHJaBm_wVBNk0jFWRQrKCNIaWITh-i35t41ED10opeItdL5FrltbSit3ns5cl_avfY_Rlaa8j6q5NukjW9y1FZn-4xwUFyWLDnK-ZM0GYXM3L7VXFac7mIH1YRc6Kzx6iT9Us5nY9oR90F_-9P_gIY7LH1</recordid><startdate>199209</startdate><enddate>199209</enddate><creator>Pham, Daphne Q.-D.</creator><creator>Sivasubramanian, Nataraian</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199209</creationdate><title>In vivo transcriptional analysis of three baculovirus genes: Evidence of homology between viral and host transcripts</title><author>Pham, Daphne Q.-D. ; Sivasubramanian, Nataraian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-cdf0f58266404a6db5567212c798833b27a744694a11a193be03d69cf6efb1fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>ARN</topic><topic>AUTOGRAPHA CALIFORNICA</topic><topic>Bacteriology</topic><topic>BACULOVIRIDAE</topic><topic>Baculoviridae - genetics</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cell Line</topic><topic>CELLULE SANGUINE</topic><topic>CELULAS SANGUINEAS</topic><topic>CICLO VITAL</topic><topic>Cloning, Molecular</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>CYCLE DE DEVELOPPEMENT</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FENOTIPOS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Viral</topic><topic>Growth, nutrition, cell differenciation</topic><topic>INFECCION</topic><topic>INFECTION</topic><topic>INTESTIN</topic><topic>INTESTINOS</topic><topic>LARVAS</topic><topic>LARVE</topic><topic>Microbiology</topic><topic>Moths</topic><topic>nuclear polyhedrosis virus</topic><topic>Occlusion Body Matrix Proteins</topic><topic>Open Reading Frames</topic><topic>PHENOTYPE</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>SPODOPTERA FRUGIPERDA</topic><topic>Transcription, Genetic</topic><topic>TRICHOPLUSIA NI</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><topic>Viral Structural Proteins</topic><topic>VIRUS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pham, Daphne Q.-D.</creatorcontrib><creatorcontrib>Sivasubramanian, Nataraian</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pham, Daphne Q.-D.</au><au>Sivasubramanian, Nataraian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo transcriptional analysis of three baculovirus genes: Evidence of homology between viral and host transcripts</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1992-09</date><risdate>1992</risdate><volume>190</volume><issue>1</issue><spage>288</spage><epage>297</epage><pages>288-297</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>Transcripts for the gp64 and polyhedrin genes as well as a 1629 open reading frame (1629 ORF) of the
Autographa californica nuclear polyhedrosis virus were examined in the midgut tissues and hemocytes of uninfected and infected host
Trichoplusia ni larvae and Sf21 cells. Polyhedrin-specific transcripts of 1.2, 3.4, and 4.9 kb were expressed in both infected larval tissues and infected Sf21 cells. The highest level of expression for polyhedrin-specific transcripts was observed in hemocytes, whereas the lowest level occurred in midgut. The expression of the 2.0-kb transcript for the gp64 gene increased continuously through 72 hr postinfection in the infected midgut tissues. This transcript was also observed in infected hemocytes, though its expression declined at 72 hr postinfection, as the expression for polyhedrin-specific transcripts peaked. The 1629 ORF transcripts of 2.0 and 3.2 kb were expressed in both types of infected tissues. More significantly, a 1629-ORF-specific probe detected host transcripts of 0.7 and 2.5 kb in uninfected midgut tissues as well as transcripts of 2.5, 8.8, and 11.0 kb in uninfected hemocytes under high stringency conditions. The latter results indicate that these host transcripts share homology with the 1629 ORF gene.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1529534</pmid><doi>10.1016/0042-6822(92)91215-G</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1992-09, Vol.190 (1), p.288-297 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73194172 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals ARN AUTOGRAPHA CALIFORNICA Bacteriology BACULOVIRIDAE Baculoviridae - genetics Biological and medical sciences Blotting, Northern Cell Line CELLULE SANGUINE CELULAS SANGUINEAS CICLO VITAL Cloning, Molecular CULTIVO DE CELULAS CULTURE DE CELLULE CYCLE DE DEVELOPPEMENT EXPRESION GENICA EXPRESSION DES GENES FENOTIPOS Fundamental and applied biological sciences. Psychology GENE GENES Genes, Viral Growth, nutrition, cell differenciation INFECCION INFECTION INTESTIN INTESTINOS LARVAS LARVE Microbiology Moths nuclear polyhedrosis virus Occlusion Body Matrix Proteins Open Reading Frames PHENOTYPE PROTEINAS PROTEINE Sequence Homology, Nucleic Acid SPODOPTERA FRUGIPERDA Transcription, Genetic TRICHOPLUSIA NI Viral Proteins - genetics Viral Proteins - metabolism Viral Structural Proteins VIRUS |
| title | In vivo transcriptional analysis of three baculovirus genes: Evidence of homology between viral and host transcripts |
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