An Automatic Flow Procedure for the Determination of 3-Hydroxybutyrate in Animal Serum and Plasma
An automatic flow procedure based on the multicommutation concept, comprising three-way solenoid valves, for the spectrophotometric determination of 3-hydroxybutyrate in animal serum and plasma is proposed. The 3-hydroxybutyrate was enzymatically converted to acetoacetate with the reduction of NAD+...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2003-04, Vol.51 (9), p.2457-2460 |
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| container_title | Journal of agricultural and food chemistry |
| container_volume | 51 |
| creator | Pires, Cherrine K. Martelli, Patrícia B. Reis, Boaventura F. Lima, José L. F. C. Saraiva, M. Lúcia M. F. S. |
| description | An automatic flow procedure based on the multicommutation concept, comprising three-way solenoid valves, for the spectrophotometric determination of 3-hydroxybutyrate in animal serum and plasma is proposed. The 3-hydroxybutyrate was enzymatically converted to acetoacetate with the reduction of NAD+ to NADH monitored at 340 nm. It was possible to carry out up to 600 determinations without a significant decrease in the analytical signal, with 5 mg of 3-hydroxybutyrate dehydrogenase immobilized on porous silica beads and packed in a column. The system enabled 60 determinations/h of 3-hydroxybutyrate in the range of 10−150 mg L-1, with a consumption of 0.9 mg of NAD+ and 200 μL of sample per determination. A detection limit of 2 mg L-1 for both animal serum and plasma and coefficients of variation of 1.4% and 1.2% (n = 17), respectively, were determined. Animal serum and plasma samples were analyzed without previous treatment, the results of which agreed with those obtained using the conventional method (UV kit, Sigma). Keywords: Flow analysis; multicommutation; 3-hydroxybutyrate; spectrophotometry; enzyme reactor; plasma and serum |
| doi_str_mv | 10.1021/jf021024n |
| format | Article |
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F. C. ; Saraiva, M. Lúcia M. F. S.</creator><creatorcontrib>Pires, Cherrine K. ; Martelli, Patrícia B. ; Reis, Boaventura F. ; Lima, José L. F. C. ; Saraiva, M. Lúcia M. F. S.</creatorcontrib><description>An automatic flow procedure based on the multicommutation concept, comprising three-way solenoid valves, for the spectrophotometric determination of 3-hydroxybutyrate in animal serum and plasma is proposed. The 3-hydroxybutyrate was enzymatically converted to acetoacetate with the reduction of NAD+ to NADH monitored at 340 nm. It was possible to carry out up to 600 determinations without a significant decrease in the analytical signal, with 5 mg of 3-hydroxybutyrate dehydrogenase immobilized on porous silica beads and packed in a column. The system enabled 60 determinations/h of 3-hydroxybutyrate in the range of 10−150 mg L-1, with a consumption of 0.9 mg of NAD+ and 200 μL of sample per determination. A detection limit of 2 mg L-1 for both animal serum and plasma and coefficients of variation of 1.4% and 1.2% (n = 17), respectively, were determined. Animal serum and plasma samples were analyzed without previous treatment, the results of which agreed with those obtained using the conventional method (UV kit, Sigma). Keywords: Flow analysis; multicommutation; 3-hydroxybutyrate; spectrophotometry; enzyme reactor; plasma and serum</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf021024n</identifier><identifier>PMID: 12696920</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>3-Hydroxybutyric Acid - blood ; 3-Hydroxybutyric Acid - metabolism ; Animals ; Biological and medical sciences ; Biosensors ; Biotechnology ; Cattle - blood ; detection limit ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Hydroxybutyrate Dehydrogenase - metabolism ; Male ; Methods. Procedures. Technologies ; NAD (coenzyme) ; Oxidation-Reduction ; Sensitivity and Specificity ; Sheep - blood ; silica ; Spectrophotometry - methods ; Temperature ; Various methods and equipments</subject><ispartof>Journal of agricultural and food chemistry, 2003-04, Vol.51 (9), p.2457-2460</ispartof><rights>Copyright © 2003 American Chemical Society</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a403t-38756441cbfaaf98d44039b4289d70b0868685f33608460e7f1b382f6298d76a3</citedby><cites>FETCH-LOGICAL-a403t-38756441cbfaaf98d44039b4289d70b0868685f33608460e7f1b382f6298d76a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf021024n$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf021024n$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14714939$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12696920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pires, Cherrine K.</creatorcontrib><creatorcontrib>Martelli, Patrícia B.</creatorcontrib><creatorcontrib>Reis, Boaventura F.</creatorcontrib><creatorcontrib>Lima, José L. F. C.</creatorcontrib><creatorcontrib>Saraiva, M. Lúcia M. F. S.</creatorcontrib><title>An Automatic Flow Procedure for the Determination of 3-Hydroxybutyrate in Animal Serum and Plasma</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>An automatic flow procedure based on the multicommutation concept, comprising three-way solenoid valves, for the spectrophotometric determination of 3-hydroxybutyrate in animal serum and plasma is proposed. The 3-hydroxybutyrate was enzymatically converted to acetoacetate with the reduction of NAD+ to NADH monitored at 340 nm. It was possible to carry out up to 600 determinations without a significant decrease in the analytical signal, with 5 mg of 3-hydroxybutyrate dehydrogenase immobilized on porous silica beads and packed in a column. The system enabled 60 determinations/h of 3-hydroxybutyrate in the range of 10−150 mg L-1, with a consumption of 0.9 mg of NAD+ and 200 μL of sample per determination. A detection limit of 2 mg L-1 for both animal serum and plasma and coefficients of variation of 1.4% and 1.2% (n = 17), respectively, were determined. Animal serum and plasma samples were analyzed without previous treatment, the results of which agreed with those obtained using the conventional method (UV kit, Sigma). Keywords: Flow analysis; multicommutation; 3-hydroxybutyrate; spectrophotometry; enzyme reactor; plasma and serum</description><subject>3-Hydroxybutyric Acid - blood</subject><subject>3-Hydroxybutyric Acid - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Cattle - blood</subject><subject>detection limit</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydroxybutyrate Dehydrogenase - metabolism</subject><subject>Male</subject><subject>Methods. Procedures. Technologies</subject><subject>NAD (coenzyme)</subject><subject>Oxidation-Reduction</subject><subject>Sensitivity and Specificity</subject><subject>Sheep - blood</subject><subject>silica</subject><subject>Spectrophotometry - methods</subject><subject>Temperature</subject><subject>Various methods and equipments</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E9rFDEYBvAgil2rB7-A5qLgYWr-J3NcqrWFpS5ui8fwzkyis84kbTJDu9_eyC7diwQSSH48SR6E3lJyRgmjn7e-zISJ8AwtqGSkkpSa52hBynZlpKIn6FXOW0KIkZq8RCeUqVrVjCwQLANezlMcYepbfDHEB7xOsXXdnBz2MeHpt8Nf3OTS2IdiYsDRY15d7roUH3fNPO0STA73JSb0Iwx449I8YggdXg-QR3iNXngYsntzWE_R7cXXm_PLavX929X5clWBIHyquNFSCUHbxgP42nSibNeNYKbuNGmIUWVIz7kiRijitKcNN8wrVqxWwE_Rx33uXYr3s8uTHfvcumGA4OKcrea05pSZAj_tYZtizsl5e5fKy9POUmL_9Wmf-iz23SF0bkbXHeWhwAI-HADkFgafILR9Pjqhqah5XVy1d32e3OPTOaQ_Vmmupb1Zb-yGXv9gP8XKyuLf772HaOFXKpm3G0aoIIRSbaQ83gxttts4p1Da_c8X_gJATZ-A</recordid><startdate>20030423</startdate><enddate>20030423</enddate><creator>Pires, Cherrine K.</creator><creator>Martelli, Patrícia B.</creator><creator>Reis, Boaventura F.</creator><creator>Lima, José L. F. C.</creator><creator>Saraiva, M. Lúcia M. F. S.</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030423</creationdate><title>An Automatic Flow Procedure for the Determination of 3-Hydroxybutyrate in Animal Serum and Plasma</title><author>Pires, Cherrine K. ; Martelli, Patrícia B. ; Reis, Boaventura F. ; Lima, José L. F. C. ; Saraiva, M. Lúcia M. F. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a403t-38756441cbfaaf98d44039b4289d70b0868685f33608460e7f1b382f6298d76a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>3-Hydroxybutyric Acid - blood</topic><topic>3-Hydroxybutyric Acid - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Cattle - blood</topic><topic>detection limit</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydroxybutyrate Dehydrogenase - metabolism</topic><topic>Male</topic><topic>Methods. Procedures. Technologies</topic><topic>NAD (coenzyme)</topic><topic>Oxidation-Reduction</topic><topic>Sensitivity and Specificity</topic><topic>Sheep - blood</topic><topic>silica</topic><topic>Spectrophotometry - methods</topic><topic>Temperature</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pires, Cherrine K.</creatorcontrib><creatorcontrib>Martelli, Patrícia B.</creatorcontrib><creatorcontrib>Reis, Boaventura F.</creatorcontrib><creatorcontrib>Lima, José L. F. C.</creatorcontrib><creatorcontrib>Saraiva, M. Lúcia M. F. S.</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pires, Cherrine K.</au><au>Martelli, Patrícia B.</au><au>Reis, Boaventura F.</au><au>Lima, José L. F. C.</au><au>Saraiva, M. Lúcia M. F. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Automatic Flow Procedure for the Determination of 3-Hydroxybutyrate in Animal Serum and Plasma</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2003-04-23</date><risdate>2003</risdate><volume>51</volume><issue>9</issue><spage>2457</spage><epage>2460</epage><pages>2457-2460</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>An automatic flow procedure based on the multicommutation concept, comprising three-way solenoid valves, for the spectrophotometric determination of 3-hydroxybutyrate in animal serum and plasma is proposed. The 3-hydroxybutyrate was enzymatically converted to acetoacetate with the reduction of NAD+ to NADH monitored at 340 nm. It was possible to carry out up to 600 determinations without a significant decrease in the analytical signal, with 5 mg of 3-hydroxybutyrate dehydrogenase immobilized on porous silica beads and packed in a column. The system enabled 60 determinations/h of 3-hydroxybutyrate in the range of 10−150 mg L-1, with a consumption of 0.9 mg of NAD+ and 200 μL of sample per determination. A detection limit of 2 mg L-1 for both animal serum and plasma and coefficients of variation of 1.4% and 1.2% (n = 17), respectively, were determined. Animal serum and plasma samples were analyzed without previous treatment, the results of which agreed with those obtained using the conventional method (UV kit, Sigma). Keywords: Flow analysis; multicommutation; 3-hydroxybutyrate; spectrophotometry; enzyme reactor; plasma and serum</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>12696920</pmid><doi>10.1021/jf021024n</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2003-04, Vol.51 (9), p.2457-2460 |
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| subjects | 3-Hydroxybutyric Acid - blood 3-Hydroxybutyric Acid - metabolism Animals Biological and medical sciences Biosensors Biotechnology Cattle - blood detection limit Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydroxybutyrate Dehydrogenase - metabolism Male Methods. Procedures. Technologies NAD (coenzyme) Oxidation-Reduction Sensitivity and Specificity Sheep - blood silica Spectrophotometry - methods Temperature Various methods and equipments |
| title | An Automatic Flow Procedure for the Determination of 3-Hydroxybutyrate in Animal Serum and Plasma |
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