Detection of cervical Chlamydia trachomatis and Neisseria gonorrhoeae with deoxyribonucleic acid probe assays in obstetric patients
OBJECTIVE: The Gen-Probe PACE 2 deoxyribonucleic acid probe assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae are targeted against the ribosomal ribonucleic acid of each pathogen. Our study compared the performance of the probe assays with culture for Chlamydia trachomatis...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1992-09, Vol.167 (3), p.588-591 |
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| container_title | American journal of obstetrics and gynecology |
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| creator | Hosein, Ian K. Kaunitz, Andrew M. Craft, Susan J. |
| description | OBJECTIVE: The Gen-Probe PACE 2 deoxyribonucleic acid probe assays for the detection of
Chlamydia trachomatis and
Neisseria gonorrhoeae are targeted against the ribosomal ribonucleic acid of each pathogen. Our study compared the performance of the probe assays with culture for
Chlamydia trachomatis (246 patients) and
Neisseria gonorrhoeae (310 patients) while screening obstetric patients.
STUDY DESIGN: Using culture as a gold standard, we assessed the sensitivity, specificity, positive predictive value, and negative predictive value of the chlamydia and gonorrhea probes.
RESULTS: The prevalence of chlamydia by culture was 13.4% and gonorrhea 4.8%. Against culture, the chlamydia probe assay performed as follows: sensitivity 93.9%, specificity 99.1%, positive predictive value 93.9%, and negative predictive value 99.1%. Values for the gonorrhea probe assay were 93.3%, 99.0%, 82.4%, and 99.7%, respectively. Additional molecular analysis of probe-positive—culture-negative specimens suggests that the gonorrhea probe-positive predictive value may be even higher.
CONCLUSION: The Gen-Probe PACE 2 deoxyribonucleic acid probe assays for chlamydia and gonorrhea appear to be promising as convenient, reliable, and cost-effective alternatives to conventional cultures in screening obstetric patients. |
| doi_str_mv | 10.1016/S0002-9378(11)91554-3 |
| format | Article |
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Chlamydia trachomatis and
Neisseria gonorrhoeae are targeted against the ribosomal ribonucleic acid of each pathogen. Our study compared the performance of the probe assays with culture for
Chlamydia trachomatis (246 patients) and
Neisseria gonorrhoeae (310 patients) while screening obstetric patients.
STUDY DESIGN: Using culture as a gold standard, we assessed the sensitivity, specificity, positive predictive value, and negative predictive value of the chlamydia and gonorrhea probes.
RESULTS: The prevalence of chlamydia by culture was 13.4% and gonorrhea 4.8%. Against culture, the chlamydia probe assay performed as follows: sensitivity 93.9%, specificity 99.1%, positive predictive value 93.9%, and negative predictive value 99.1%. Values for the gonorrhea probe assay were 93.3%, 99.0%, 82.4%, and 99.7%, respectively. Additional molecular analysis of probe-positive—culture-negative specimens suggests that the gonorrhea probe-positive predictive value may be even higher.
CONCLUSION: The Gen-Probe PACE 2 deoxyribonucleic acid probe assays for chlamydia and gonorrhea appear to be promising as convenient, reliable, and cost-effective alternatives to conventional cultures in screening obstetric patients.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/S0002-9378(11)91554-3</identifier><identifier>PMID: 1530008</identifier><language>eng</language><publisher>ST LOUIS: Mosby, Inc</publisher><subject>Adult ; Cervix Uteri - microbiology ; chlamydia ; Chlamydia trachomatis ; Chlamydia trachomatis - isolation & purification ; deoxyribonucleic acid probes ; DNA Probes ; Female ; Gonorrhea ; Humans ; Life Sciences & Biomedicine ; Neisseria gonorrhoeae - isolation & purification ; Nucleic Acid Hybridization ; Obstetrics & Gynecology ; Pregnancy ; Pregnancy Complications, Infectious - microbiology ; Science & Technology ; Sensitivity and Specificity</subject><ispartof>American journal of obstetrics and gynecology, 1992-09, Vol.167 (3), p.588-591</ispartof><rights>1992 Mosby</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>18</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wosA1992JN79000003</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c391t-fd72a3a0ad387826dcf91eef5588ca8360d93723294fb5fa79fe2c4a31dac3943</citedby><cites>FETCH-LOGICAL-c391t-fd72a3a0ad387826dcf91eef5588ca8360d93723294fb5fa79fe2c4a31dac3943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0002-9378(11)91554-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27197,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1530008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hosein, Ian K.</creatorcontrib><creatorcontrib>Kaunitz, Andrew M.</creatorcontrib><creatorcontrib>Craft, Susan J.</creatorcontrib><title>Detection of cervical Chlamydia trachomatis and Neisseria gonorrhoeae with deoxyribonucleic acid probe assays in obstetric patients</title><title>American journal of obstetrics and gynecology</title><addtitle>AM J OBSTET GYNECOL</addtitle><addtitle>Am J Obstet Gynecol</addtitle><description>OBJECTIVE: The Gen-Probe PACE 2 deoxyribonucleic acid probe assays for the detection of
Chlamydia trachomatis and
Neisseria gonorrhoeae are targeted against the ribosomal ribonucleic acid of each pathogen. Our study compared the performance of the probe assays with culture for
Chlamydia trachomatis (246 patients) and
Neisseria gonorrhoeae (310 patients) while screening obstetric patients.
STUDY DESIGN: Using culture as a gold standard, we assessed the sensitivity, specificity, positive predictive value, and negative predictive value of the chlamydia and gonorrhea probes.
RESULTS: The prevalence of chlamydia by culture was 13.4% and gonorrhea 4.8%. Against culture, the chlamydia probe assay performed as follows: sensitivity 93.9%, specificity 99.1%, positive predictive value 93.9%, and negative predictive value 99.1%. Values for the gonorrhea probe assay were 93.3%, 99.0%, 82.4%, and 99.7%, respectively. Additional molecular analysis of probe-positive—culture-negative specimens suggests that the gonorrhea probe-positive predictive value may be even higher.
CONCLUSION: The Gen-Probe PACE 2 deoxyribonucleic acid probe assays for chlamydia and gonorrhea appear to be promising as convenient, reliable, and cost-effective alternatives to conventional cultures in screening obstetric patients.</description><subject>Adult</subject><subject>Cervix Uteri - microbiology</subject><subject>chlamydia</subject><subject>Chlamydia trachomatis</subject><subject>Chlamydia trachomatis - isolation & purification</subject><subject>deoxyribonucleic acid probes</subject><subject>DNA Probes</subject><subject>Female</subject><subject>Gonorrhea</subject><subject>Humans</subject><subject>Life Sciences & Biomedicine</subject><subject>Neisseria gonorrhoeae - isolation & purification</subject><subject>Nucleic Acid Hybridization</subject><subject>Obstetrics & Gynecology</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Infectious - microbiology</subject><subject>Science & Technology</subject><subject>Sensitivity and Specificity</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EZCTM</sourceid><sourceid>EIF</sourceid><recordid>eNqNUktv1DAQthCoLIWfUMknRIUCdrxJ7BOqwltVOQBna2JPWKMkXmynZc_8cZxmVY7FF2v0PcYznwk54-wVZ7x-_ZUxVhZKNPIF5-eKV9W2EA_IhjPVFLWs5UOyuaM8Jk9i_LmUpSpPyAmvRC7khvx5iwlNcn6ivqcGw7UzMNB2N8B4sA5oCmB2foTkIoXJ0it0MWLIyA8_-RB2HgHpjUs7atH_PgTX-Wk2AzpDwThL98F3SCFGOETqcpsuJkwhw_tsilOKT8mjHoaIz473Kfn-_t239mNx-eXDp_bisjBC8VT0tilBAAMrZCPL2ppeccS-qqQ0IEXNbB61FKXa9l3VQ6N6LM0WBLeQHbbilDxfffOTfs0Ykx5dNDgMMKGfo24EV1zVzb1EXtc1k0JmYrUSTfAxBuz1PrgRwkFzppeU9G1KeolAc65vU9Ii686ODeZuRPtPtcaS8ZcrfoOd76PJazJ4x7rgSpWfrxrFlrO4yf9nty7BEnfr5yll6ZtVinnv1w6DPsqtC_lbaOvdPaP8BWdYxhM</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Hosein, Ian K.</creator><creator>Kaunitz, Andrew M.</creator><creator>Craft, Susan J.</creator><general>Mosby, Inc</general><general>MOSBY-YEAR BOOK INC</general><scope>BLEPL</scope><scope>DTL</scope><scope>EZCTM</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19920901</creationdate><title>Detection of cervical Chlamydia trachomatis and Neisseria gonorrhoeae with deoxyribonucleic acid probe assays in obstetric patients</title><author>Hosein, Ian K. ; Kaunitz, Andrew M. ; Craft, Susan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-fd72a3a0ad387826dcf91eef5588ca8360d93723294fb5fa79fe2c4a31dac3943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adult</topic><topic>Cervix Uteri - microbiology</topic><topic>chlamydia</topic><topic>Chlamydia trachomatis</topic><topic>Chlamydia trachomatis - isolation & purification</topic><topic>deoxyribonucleic acid probes</topic><topic>DNA Probes</topic><topic>Female</topic><topic>Gonorrhea</topic><topic>Humans</topic><topic>Life Sciences & Biomedicine</topic><topic>Neisseria gonorrhoeae - isolation & purification</topic><topic>Nucleic Acid Hybridization</topic><topic>Obstetrics & Gynecology</topic><topic>Pregnancy</topic><topic>Pregnancy Complications, Infectious - microbiology</topic><topic>Science & Technology</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hosein, Ian K.</creatorcontrib><creatorcontrib>Kaunitz, Andrew M.</creatorcontrib><creatorcontrib>Craft, Susan J.</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hosein, Ian K.</au><au>Kaunitz, Andrew M.</au><au>Craft, Susan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of cervical Chlamydia trachomatis and Neisseria gonorrhoeae with deoxyribonucleic acid probe assays in obstetric patients</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><stitle>AM J OBSTET GYNECOL</stitle><addtitle>Am J Obstet Gynecol</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>167</volume><issue>3</issue><spage>588</spage><epage>591</epage><pages>588-591</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><abstract>OBJECTIVE: The Gen-Probe PACE 2 deoxyribonucleic acid probe assays for the detection of
Chlamydia trachomatis and
Neisseria gonorrhoeae are targeted against the ribosomal ribonucleic acid of each pathogen. Our study compared the performance of the probe assays with culture for
Chlamydia trachomatis (246 patients) and
Neisseria gonorrhoeae (310 patients) while screening obstetric patients.
STUDY DESIGN: Using culture as a gold standard, we assessed the sensitivity, specificity, positive predictive value, and negative predictive value of the chlamydia and gonorrhea probes.
RESULTS: The prevalence of chlamydia by culture was 13.4% and gonorrhea 4.8%. Against culture, the chlamydia probe assay performed as follows: sensitivity 93.9%, specificity 99.1%, positive predictive value 93.9%, and negative predictive value 99.1%. Values for the gonorrhea probe assay were 93.3%, 99.0%, 82.4%, and 99.7%, respectively. Additional molecular analysis of probe-positive—culture-negative specimens suggests that the gonorrhea probe-positive predictive value may be even higher.
CONCLUSION: The Gen-Probe PACE 2 deoxyribonucleic acid probe assays for chlamydia and gonorrhea appear to be promising as convenient, reliable, and cost-effective alternatives to conventional cultures in screening obstetric patients.</abstract><cop>ST LOUIS</cop><pub>Mosby, Inc</pub><pmid>1530008</pmid><doi>10.1016/S0002-9378(11)91554-3</doi><tpages>4</tpages></addata></record> |
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| issn | 0002-9378 1097-6868 |
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| source | Web of Science - Science Citation Index Expanded - 1992<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adult Cervix Uteri - microbiology chlamydia Chlamydia trachomatis Chlamydia trachomatis - isolation & purification deoxyribonucleic acid probes DNA Probes Female Gonorrhea Humans Life Sciences & Biomedicine Neisseria gonorrhoeae - isolation & purification Nucleic Acid Hybridization Obstetrics & Gynecology Pregnancy Pregnancy Complications, Infectious - microbiology Science & Technology Sensitivity and Specificity |
| title | Detection of cervical Chlamydia trachomatis and Neisseria gonorrhoeae with deoxyribonucleic acid probe assays in obstetric patients |
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