Effects of hyaluronic acid-carboxymethylcellulose antiadhesion barrier on ischemic colonic anastomosis: An experimental study
Intraperitoneal adhesions may help the healing of marginally viable bowel ends. If adhesion formation is prevented by various methods, the integrity of ischemic bowel anastomosis may be compromised. Thus, we decided to study the effects of hyaluronic acid-carboxymethylcellulose, an antiadhesion barr...
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| Veröffentlicht in: | Diseases of the colon & rectum 2003-04, Vol.46 (4), p.529-534 |
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| creator | ERTURK, Suphan YUCEYAR, Serdar TEMIZ, Muhyittin EKCI, Baki SAKOGLU, Nevin BALCI, Huriye DIRICAN, Ahmet CENGIZ, Ali SANER, Haluk |
| description | Intraperitoneal adhesions may help the healing of marginally viable bowel ends. If adhesion formation is prevented by various methods, the integrity of ischemic bowel anastomosis may be compromised. Thus, we decided to study the effects of hyaluronic acid-carboxymethylcellulose, an antiadhesion barrier, on ischemic bowel anastomosis.
Thirty Wistar-Albino rats were divided into three groups. In Group A (control), a well-perfused distal colonic segment was transected, and free ends were anastomosed. In Group B, an ischemic colonic segment was prepared, then divided and anastomosed. In Group C, after completion of ischemic colonic anastomosis, hyaluronic acid-carboxymethylcellulose film was wrapped around the anastomosis. In all groups, rats were killed on the seventh day. Intraperitoneal adhesions were graded by adhesion score, and healing of the anastomosis was assessed by measurement of bursting pressure and hydroxyproline levels in the anastomotic tissue.
A statistically significant difference was found between hydroxyproline levels of the control group and the ischemic group (P = 0.02). HP level was also significantly higher in the hyaluronic acid-carboxymethylcellulose group than in the ischemic group (P = 0.01). There was no difference in hydroxyproline levels between the control and hyaluronic acid-carboxymethylcellulose groups. Compared with the control group, bursting pressure was lower in the ischemic group (P = 0.02). Hyaluronic acid-carboxymethylcellulose wrapping increased the bursting pressure significantly (P < 0.001). However, there was no difference in bursting pressure between the control group and the hyaluronic acid-carboxymethylcellulose group (P = 0.13). A marked increase in the adhesion score was observed in the ischemic group (P = 0.01). The difference between adhesion scores of the hyaluronic acid-carboxymethylcellulose and ischemic groups was not found to be significant, although the adhesion score in the hyaluronic acid-carboxymethylcellulose group was lower (P = 0.16). There was no difference in adhesion score between the control and hyaluronic acid-carboxymethylcellulose groups.
Application of hyaluronic acid-carboxymethylcellulose in ischemic colonic anastomosis did not compromise anastomotic integrity. The adverse effect of ischemia on healing of colonic anastomosis was counteracted by hyaluronic acid-carboxymethylcellulose. |
| doi_str_mv | 10.1007/s10350-004-6594-1 |
| format | Article |
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Thirty Wistar-Albino rats were divided into three groups. In Group A (control), a well-perfused distal colonic segment was transected, and free ends were anastomosed. In Group B, an ischemic colonic segment was prepared, then divided and anastomosed. In Group C, after completion of ischemic colonic anastomosis, hyaluronic acid-carboxymethylcellulose film was wrapped around the anastomosis. In all groups, rats were killed on the seventh day. Intraperitoneal adhesions were graded by adhesion score, and healing of the anastomosis was assessed by measurement of bursting pressure and hydroxyproline levels in the anastomotic tissue.
A statistically significant difference was found between hydroxyproline levels of the control group and the ischemic group (P = 0.02). HP level was also significantly higher in the hyaluronic acid-carboxymethylcellulose group than in the ischemic group (P = 0.01). There was no difference in hydroxyproline levels between the control and hyaluronic acid-carboxymethylcellulose groups. Compared with the control group, bursting pressure was lower in the ischemic group (P = 0.02). Hyaluronic acid-carboxymethylcellulose wrapping increased the bursting pressure significantly (P < 0.001). However, there was no difference in bursting pressure between the control group and the hyaluronic acid-carboxymethylcellulose group (P = 0.13). A marked increase in the adhesion score was observed in the ischemic group (P = 0.01). The difference between adhesion scores of the hyaluronic acid-carboxymethylcellulose and ischemic groups was not found to be significant, although the adhesion score in the hyaluronic acid-carboxymethylcellulose group was lower (P = 0.16). There was no difference in adhesion score between the control and hyaluronic acid-carboxymethylcellulose groups.
Application of hyaluronic acid-carboxymethylcellulose in ischemic colonic anastomosis did not compromise anastomotic integrity. The adverse effect of ischemia on healing of colonic anastomosis was counteracted by hyaluronic acid-carboxymethylcellulose.</description><identifier>ISSN: 0012-3706</identifier><identifier>EISSN: 1530-0358</identifier><identifier>DOI: 10.1007/s10350-004-6594-1</identifier><identifier>PMID: 12682549</identifier><identifier>CODEN: DICRAG</identifier><language>eng</language><publisher>Secaucus, NJ: Springer</publisher><subject>Anastomosis, Surgical ; Animals ; Biocompatible Materials - pharmacology ; Biocompatible Materials - therapeutic use ; Biological and medical sciences ; Cellulase ; Colon - surgery ; Female ; Glycoside Hydrolases - pharmacology ; Glycoside Hydrolases - therapeutic use ; Hyaluronic Acid - pharmacology ; Hyaluronic Acid - therapeutic use ; Medical sciences ; Membranes, Artificial ; Rats ; Rats, Wistar ; Stomach, duodenum, intestine, rectum, anus ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the digestive system ; Tensile Strength ; Tissue Adhesions - prevention & control ; Wound Healing - drug effects</subject><ispartof>Diseases of the colon & rectum, 2003-04, Vol.46 (4), p.529-534</ispartof><rights>2003 INIST-CNRS</rights><rights>The American Society of Colon and Rectal Surgeons 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23909,23910,25118,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14720598$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12682549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ERTURK, Suphan</creatorcontrib><creatorcontrib>YUCEYAR, Serdar</creatorcontrib><creatorcontrib>TEMIZ, Muhyittin</creatorcontrib><creatorcontrib>EKCI, Baki</creatorcontrib><creatorcontrib>SAKOGLU, Nevin</creatorcontrib><creatorcontrib>BALCI, Huriye</creatorcontrib><creatorcontrib>DIRICAN, Ahmet</creatorcontrib><creatorcontrib>CENGIZ, Ali</creatorcontrib><creatorcontrib>SANER, Haluk</creatorcontrib><title>Effects of hyaluronic acid-carboxymethylcellulose antiadhesion barrier on ischemic colonic anastomosis: An experimental study</title><title>Diseases of the colon & rectum</title><addtitle>Dis Colon Rectum</addtitle><description>Intraperitoneal adhesions may help the healing of marginally viable bowel ends. If adhesion formation is prevented by various methods, the integrity of ischemic bowel anastomosis may be compromised. Thus, we decided to study the effects of hyaluronic acid-carboxymethylcellulose, an antiadhesion barrier, on ischemic bowel anastomosis.
Thirty Wistar-Albino rats were divided into three groups. In Group A (control), a well-perfused distal colonic segment was transected, and free ends were anastomosed. In Group B, an ischemic colonic segment was prepared, then divided and anastomosed. In Group C, after completion of ischemic colonic anastomosis, hyaluronic acid-carboxymethylcellulose film was wrapped around the anastomosis. In all groups, rats were killed on the seventh day. Intraperitoneal adhesions were graded by adhesion score, and healing of the anastomosis was assessed by measurement of bursting pressure and hydroxyproline levels in the anastomotic tissue.
A statistically significant difference was found between hydroxyproline levels of the control group and the ischemic group (P = 0.02). HP level was also significantly higher in the hyaluronic acid-carboxymethylcellulose group than in the ischemic group (P = 0.01). There was no difference in hydroxyproline levels between the control and hyaluronic acid-carboxymethylcellulose groups. Compared with the control group, bursting pressure was lower in the ischemic group (P = 0.02). Hyaluronic acid-carboxymethylcellulose wrapping increased the bursting pressure significantly (P < 0.001). However, there was no difference in bursting pressure between the control group and the hyaluronic acid-carboxymethylcellulose group (P = 0.13). A marked increase in the adhesion score was observed in the ischemic group (P = 0.01). The difference between adhesion scores of the hyaluronic acid-carboxymethylcellulose and ischemic groups was not found to be significant, although the adhesion score in the hyaluronic acid-carboxymethylcellulose group was lower (P = 0.16). There was no difference in adhesion score between the control and hyaluronic acid-carboxymethylcellulose groups.
Application of hyaluronic acid-carboxymethylcellulose in ischemic colonic anastomosis did not compromise anastomotic integrity. The adverse effect of ischemia on healing of colonic anastomosis was counteracted by hyaluronic acid-carboxymethylcellulose.</description><subject>Anastomosis, Surgical</subject><subject>Animals</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biocompatible Materials - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Cellulase</subject><subject>Colon - surgery</subject><subject>Female</subject><subject>Glycoside Hydrolases - pharmacology</subject><subject>Glycoside Hydrolases - therapeutic use</subject><subject>Hyaluronic Acid - pharmacology</subject><subject>Hyaluronic Acid - therapeutic use</subject><subject>Medical sciences</subject><subject>Membranes, Artificial</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Stomach, duodenum, intestine, rectum, anus</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the digestive system</subject><subject>Tensile Strength</subject><subject>Tissue Adhesions - prevention & control</subject><subject>Wound Healing - drug effects</subject><issn>0012-3706</issn><issn>1530-0358</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtr3TAQhUVpSW7T_IBuiimkO7cj62V3F0IehUA37VqMZYmrIEu3kg3xov-9CvdCoKvRiO8cznAI-UjhKwVQ3woFJqAF4K0UA2_pG7KjgtUfJvq3ZAdAu5YpkOfkfSlPdYUO1Bk5p53sO8GHHfl765w1S2mSa_YbhjWn6E2Dxk-twTym5222y34LxoawhlRsg3HxOO1t8Sk2I-bsbW7q0xezt3MVmxSOJhHLkuZUfPneXMfGPh9s9rONC4amLOu0fSDvHIZiL0_zgvy-u_1189A-_rz_cXP92BoGcqlBJhSKWuTAlDRWjsBoxwU61SvoR9cBcjfiCBNXiqmBG-MmlHSikgvF2AX5cvQ95PRntWXRc01bL8Jo01q0YrSXivYV_Pwf-JTWHGs23VEOkvYwVIgeIZNTKdk6fahnYd40Bf1SjD4Wo2sx-qUYTavm08l4HWc7vSpOTVTg6gRgMRhcxmh8eeW46kAMPfsHzp-YVA</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>ERTURK, Suphan</creator><creator>YUCEYAR, Serdar</creator><creator>TEMIZ, Muhyittin</creator><creator>EKCI, Baki</creator><creator>SAKOGLU, Nevin</creator><creator>BALCI, Huriye</creator><creator>DIRICAN, Ahmet</creator><creator>CENGIZ, Ali</creator><creator>SANER, Haluk</creator><general>Springer</general><general>Lippincott Williams & Wilkins Ovid Technologies</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Effects of hyaluronic acid-carboxymethylcellulose antiadhesion barrier on ischemic colonic anastomosis: An experimental study</title><author>ERTURK, Suphan ; YUCEYAR, Serdar ; TEMIZ, Muhyittin ; EKCI, Baki ; SAKOGLU, Nevin ; BALCI, Huriye ; DIRICAN, Ahmet ; CENGIZ, Ali ; SANER, Haluk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-cada571ea40376ce6b031245af78708bf20a4fbab0d4773794ccfda61d1645733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Anastomosis, Surgical</topic><topic>Animals</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biocompatible Materials - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Cellulase</topic><topic>Colon - surgery</topic><topic>Female</topic><topic>Glycoside Hydrolases - pharmacology</topic><topic>Glycoside Hydrolases - therapeutic use</topic><topic>Hyaluronic Acid - pharmacology</topic><topic>Hyaluronic Acid - therapeutic use</topic><topic>Medical sciences</topic><topic>Membranes, Artificial</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Stomach, duodenum, intestine, rectum, anus</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the digestive system</topic><topic>Tensile Strength</topic><topic>Tissue Adhesions - prevention & control</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ERTURK, Suphan</creatorcontrib><creatorcontrib>YUCEYAR, Serdar</creatorcontrib><creatorcontrib>TEMIZ, Muhyittin</creatorcontrib><creatorcontrib>EKCI, Baki</creatorcontrib><creatorcontrib>SAKOGLU, Nevin</creatorcontrib><creatorcontrib>BALCI, Huriye</creatorcontrib><creatorcontrib>DIRICAN, Ahmet</creatorcontrib><creatorcontrib>CENGIZ, Ali</creatorcontrib><creatorcontrib>SANER, Haluk</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Diseases of the colon & rectum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ERTURK, Suphan</au><au>YUCEYAR, Serdar</au><au>TEMIZ, Muhyittin</au><au>EKCI, Baki</au><au>SAKOGLU, Nevin</au><au>BALCI, Huriye</au><au>DIRICAN, Ahmet</au><au>CENGIZ, Ali</au><au>SANER, Haluk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of hyaluronic acid-carboxymethylcellulose antiadhesion barrier on ischemic colonic anastomosis: An experimental study</atitle><jtitle>Diseases of the colon & rectum</jtitle><addtitle>Dis Colon Rectum</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>46</volume><issue>4</issue><spage>529</spage><epage>534</epage><pages>529-534</pages><issn>0012-3706</issn><eissn>1530-0358</eissn><coden>DICRAG</coden><abstract>Intraperitoneal adhesions may help the healing of marginally viable bowel ends. If adhesion formation is prevented by various methods, the integrity of ischemic bowel anastomosis may be compromised. Thus, we decided to study the effects of hyaluronic acid-carboxymethylcellulose, an antiadhesion barrier, on ischemic bowel anastomosis.
Thirty Wistar-Albino rats were divided into three groups. In Group A (control), a well-perfused distal colonic segment was transected, and free ends were anastomosed. In Group B, an ischemic colonic segment was prepared, then divided and anastomosed. In Group C, after completion of ischemic colonic anastomosis, hyaluronic acid-carboxymethylcellulose film was wrapped around the anastomosis. In all groups, rats were killed on the seventh day. Intraperitoneal adhesions were graded by adhesion score, and healing of the anastomosis was assessed by measurement of bursting pressure and hydroxyproline levels in the anastomotic tissue.
A statistically significant difference was found between hydroxyproline levels of the control group and the ischemic group (P = 0.02). HP level was also significantly higher in the hyaluronic acid-carboxymethylcellulose group than in the ischemic group (P = 0.01). There was no difference in hydroxyproline levels between the control and hyaluronic acid-carboxymethylcellulose groups. Compared with the control group, bursting pressure was lower in the ischemic group (P = 0.02). Hyaluronic acid-carboxymethylcellulose wrapping increased the bursting pressure significantly (P < 0.001). However, there was no difference in bursting pressure between the control group and the hyaluronic acid-carboxymethylcellulose group (P = 0.13). A marked increase in the adhesion score was observed in the ischemic group (P = 0.01). The difference between adhesion scores of the hyaluronic acid-carboxymethylcellulose and ischemic groups was not found to be significant, although the adhesion score in the hyaluronic acid-carboxymethylcellulose group was lower (P = 0.16). There was no difference in adhesion score between the control and hyaluronic acid-carboxymethylcellulose groups.
Application of hyaluronic acid-carboxymethylcellulose in ischemic colonic anastomosis did not compromise anastomotic integrity. The adverse effect of ischemia on healing of colonic anastomosis was counteracted by hyaluronic acid-carboxymethylcellulose.</abstract><cop>Secaucus, NJ</cop><pub>Springer</pub><pmid>12682549</pmid><doi>10.1007/s10350-004-6594-1</doi><tpages>6</tpages></addata></record> |
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| subjects | Anastomosis, Surgical Animals Biocompatible Materials - pharmacology Biocompatible Materials - therapeutic use Biological and medical sciences Cellulase Colon - surgery Female Glycoside Hydrolases - pharmacology Glycoside Hydrolases - therapeutic use Hyaluronic Acid - pharmacology Hyaluronic Acid - therapeutic use Medical sciences Membranes, Artificial Rats Rats, Wistar Stomach, duodenum, intestine, rectum, anus Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the digestive system Tensile Strength Tissue Adhesions - prevention & control Wound Healing - drug effects |
| title | Effects of hyaluronic acid-carboxymethylcellulose antiadhesion barrier on ischemic colonic anastomosis: An experimental study |
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