Amphotericin B-Induced Carbohydrate Changes on the Trypanosoma cruzi Surface Membrane
ABSTRACT Changes in the cell surface carbohydrates of Trypanosoma cruzi epimastigotes induced by Amphotericin B (AmB) were assessed by chemical methods and by agglutination assay employing a panel of highly purified lectins of various sugar specificities, Escherichia coli K12 with mannose‐sensitive...
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| Veröffentlicht in: | The Journal of Protozoology 1992-09, Vol.39 (5), p.609-612 |
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| creator | HAIDO, ROSA M. T. ESTEVES, MANUEL J. G. BARRETO-BERGTER, ELIANA |
| description | ABSTRACT Changes in the cell surface carbohydrates of Trypanosoma cruzi epimastigotes induced by Amphotericin B (AmB) were assessed by chemical methods and by agglutination assay employing a panel of highly purified lectins of various sugar specificities, Escherichia coli K12 with mannose‐sensitive fimbriae was also used as an agglutination probe. Amphotericin B caused a decrease in the total carbohydrate content of all glycoconjugate fractions isolated. Exposure to AmB strongly affected the mannose/galactose ratio (1:5) in the CHCI3/methanol/H2O soluble fraction. These sugars in 1.4:1 ratio were the major hexose components of control cells. The decrease in the mannose content (48 to 15%) after AmB treatment agrees with the marked decrease in the T. cruzi cell surface receptors for fimbriated E. coli K12. Also, an increase in the galactose content (74%) as compared with control cells (34%) is in agreement with the peanut agglutinin and Euonymus europaeus lectins agglutination results. Differences in the cell surface carbohydrates induced by AmB could be associated with alterations in the membrane structure and organization. |
| doi_str_mv | 10.1111/j.1550-7408.1992.tb04859.x |
| format | Article |
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Also, an increase in the galactose content (74%) as compared with control cells (34%) is in agreement with the peanut agglutinin and Euonymus europaeus lectins agglutination results. Differences in the cell surface carbohydrates induced by AmB could be associated with alterations in the membrane structure and organization.</description><identifier>ISSN: 0022-3921</identifier><identifier>EISSN: 1550-7408</identifier><identifier>EISSN: 2375-0804</identifier><identifier>DOI: 10.1111/j.1550-7408.1992.tb04859.x</identifier><identifier>PMID: 1522543</identifier><identifier>CODEN: JPROAR</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Agglutination Tests ; Amphotericin B - pharmacology ; Animals ; Antibiotics. Antiinfectious agents. 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T.</creatorcontrib><creatorcontrib>ESTEVES, MANUEL J. G.</creatorcontrib><creatorcontrib>BARRETO-BERGTER, ELIANA</creatorcontrib><title>Amphotericin B-Induced Carbohydrate Changes on the Trypanosoma cruzi Surface Membrane</title><title>The Journal of Protozoology</title><addtitle>J Protozool</addtitle><description>ABSTRACT Changes in the cell surface carbohydrates of Trypanosoma cruzi epimastigotes induced by Amphotericin B (AmB) were assessed by chemical methods and by agglutination assay employing a panel of highly purified lectins of various sugar specificities, Escherichia coli K12 with mannose‐sensitive fimbriae was also used as an agglutination probe. Amphotericin B caused a decrease in the total carbohydrate content of all glycoconjugate fractions isolated. Exposure to AmB strongly affected the mannose/galactose ratio (1:5) in the CHCI3/methanol/H2O soluble fraction. These sugars in 1.4:1 ratio were the major hexose components of control cells. The decrease in the mannose content (48 to 15%) after AmB treatment agrees with the marked decrease in the T. cruzi cell surface receptors for fimbriated E. coli K12. Also, an increase in the galactose content (74%) as compared with control cells (34%) is in agreement with the peanut agglutinin and Euonymus europaeus lectins agglutination results. Differences in the cell surface carbohydrates induced by AmB could be associated with alterations in the membrane structure and organization.</description><subject>Agglutination Tests</subject><subject>Amphotericin B - pharmacology</subject><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Metabolism</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Glycoconjugates</subject><subject>lectins</subject><subject>Lectins - metabolism</subject><subject>Medical sciences</subject><subject>Miscellaneous. Antibiotics with multiple activities</subject><subject>Pharmacology. Drug treatments</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - drug effects</subject><issn>0022-3921</issn><issn>1550-7408</issn><issn>2375-0804</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkN2L1DAUxYMo6-zqnyAEEd9ac_PZ-CCs4-66sirqDD6GNE2djv0Yk5ad8a-3pcP4KF4I9-Gce3L4IfQcSArjvNqmIARJFCdZClrTtM8Jz4RO9w_Q4iQ9RAtCKE2YpvAYnce4JYRRLuEMnYGgVHC2QOvLZrfpeh8qV7X4bXLbFoPzBV7akHebQxFs7_FyY9sfPuKuxf3G41U47Gzbxa6x2IXhd4W_DaG0zuOPvsmDbf0T9Ki0dfRPj_sCra-vVsv3yd3nm9vl5V3iuFQ6UZY5cMIT5akGpksAkZVQ2ByIF6AZV7aUGfHMOVJorSWTICSVxfiEA3aBXs65u9D9GnzsTVNF5-t67NAN0SgGGaU8-6cRJKMUlBqNr2ejC12MwZdmF6rGhoMBYib4ZmsmwmYibCb45gjf7MfjZ8dfhrzxxd_TmfaovzjqNjpblyMqV8WTjXPQlE1l38y2-6r2h_8oYD5crSXRY0AyB1Sx9_tTgA0_jVRMCfP90435wr9eE5G9Myv2BzBLrsc</recordid><startdate>199209</startdate><enddate>199209</enddate><creator>HAIDO, ROSA M. T.</creator><creator>ESTEVES, MANUEL J. G.</creator><creator>BARRETO-BERGTER, ELIANA</creator><general>Blackwell Publishing Ltd</general><general>Society of Protozoologists</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>199209</creationdate><title>Amphotericin B-Induced Carbohydrate Changes on the Trypanosoma cruzi Surface Membrane</title><author>HAIDO, ROSA M. T. ; ESTEVES, MANUEL J. G. ; BARRETO-BERGTER, ELIANA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4679-7a3c1c5e07e29139f1158f1dab10e519347af680e3cc0d99963615626d6265c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Agglutination Tests</topic><topic>Amphotericin B - pharmacology</topic><topic>Animals</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Metabolism</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Glycoconjugates</topic><topic>lectins</topic><topic>Lectins - metabolism</topic><topic>Medical sciences</topic><topic>Miscellaneous. Antibiotics with multiple activities</topic><topic>Pharmacology. Drug treatments</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HAIDO, ROSA M. T.</creatorcontrib><creatorcontrib>ESTEVES, MANUEL J. G.</creatorcontrib><creatorcontrib>BARRETO-BERGTER, ELIANA</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of Protozoology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HAIDO, ROSA M. T.</au><au>ESTEVES, MANUEL J. G.</au><au>BARRETO-BERGTER, ELIANA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amphotericin B-Induced Carbohydrate Changes on the Trypanosoma cruzi Surface Membrane</atitle><jtitle>The Journal of Protozoology</jtitle><addtitle>J Protozool</addtitle><date>1992-09</date><risdate>1992</risdate><volume>39</volume><issue>5</issue><spage>609</spage><epage>612</epage><pages>609-612</pages><issn>0022-3921</issn><eissn>1550-7408</eissn><eissn>2375-0804</eissn><coden>JPROAR</coden><abstract>ABSTRACT Changes in the cell surface carbohydrates of Trypanosoma cruzi epimastigotes induced by Amphotericin B (AmB) were assessed by chemical methods and by agglutination assay employing a panel of highly purified lectins of various sugar specificities, Escherichia coli K12 with mannose‐sensitive fimbriae was also used as an agglutination probe. Amphotericin B caused a decrease in the total carbohydrate content of all glycoconjugate fractions isolated. Exposure to AmB strongly affected the mannose/galactose ratio (1:5) in the CHCI3/methanol/H2O soluble fraction. These sugars in 1.4:1 ratio were the major hexose components of control cells. The decrease in the mannose content (48 to 15%) after AmB treatment agrees with the marked decrease in the T. cruzi cell surface receptors for fimbriated E. coli K12. Also, an increase in the galactose content (74%) as compared with control cells (34%) is in agreement with the peanut agglutinin and Euonymus europaeus lectins agglutination results. Differences in the cell surface carbohydrates induced by AmB could be associated with alterations in the membrane structure and organization.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1522543</pmid><doi>10.1111/j.1550-7408.1992.tb04859.x</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Agglutination Tests Amphotericin B - pharmacology Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Biological and medical sciences Carbohydrate Metabolism Cell Membrane - drug effects Cell Membrane - metabolism Glycoconjugates lectins Lectins - metabolism Medical sciences Miscellaneous. Antibiotics with multiple activities Pharmacology. Drug treatments Trypanosoma cruzi Trypanosoma cruzi - drug effects |
| title | Amphotericin B-Induced Carbohydrate Changes on the Trypanosoma cruzi Surface Membrane |
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