Evaluation of Extraction Methodologies for Corn Kernel (Zea mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA
Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were evaluated for extracting DNA from ground corn kernels s...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2003-04, Vol.51 (9), p.2468-2474 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2474 |
|---|---|
| container_issue | 9 |
| container_start_page | 2468 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 51 |
| creator | Holden, Marcia J. Blasic, Joseph R. Bussjaeger, Louis Kao, Chuan Shokere, Luke A. Kendall, Donald C. Freese, Larry Jenkins, G. Ronald |
| description | Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were evaluated for extracting DNA from ground corn kernels spiked with 0.1% (m/m) CBH351 (StarLink) corn. DNA preparations were evaluated for purity and fragment size. Extraction efficiency was determined. The alcohol dehydrogenase gene (adh1) and the CBH351 (cry9C, 35S promoter) genes in the genomic DNA were detected using PCR. DNA isolated by two of the methods proved unsuitable for performing PCR amplification. All other methods produced some DNA preparations that gave false negative PCR results. We observed that cornstarch, a primary component of corn kernels, was not an inhibitor of PCR, while acidic polysaccharides were. Our data suggest that amplification of an endogenous positive control gene, as an indicator for the absence of PCR inhibitors, is not always valid. This study points out aspects of DNA isolation that need to be considered when choosing a method for a particular plant/tissue type. Keywords: Zea mays; corn; genomic; DNA; extraction; polymerase chain reaction; PCR; PCR inhibition |
| doi_str_mv | 10.1021/jf0211130 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73181299</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17586749</sourcerecordid><originalsourceid>FETCH-LOGICAL-a434t-f64dfa64f3dd7b07c68186831877a7de46eabc89a0850a13e9a447279b72b0033</originalsourceid><addsrcrecordid>eNqFks9v0zAUxy0EYmVw4B-AXEDsEPBzHDs-lrYDxPgx1l24WK-JvaVL4mEn03rkP8dZy3pB4mLL7338sZ6-JuQ50LdAGbxb27gCZPQBmUDOaJoDFA_JhMZyWuQCDsiTENaU0iKX9DE5ACaUUIxNyO_FDTYD9rXrEmeTxW3vsbw7fTH9patc4y5qExLrfDJzvks-G9-ZJnnz02DS4iYcJfOv07v23PSm_CtaRo1Jpq0buj6Mhfe1i-3LbhRu0rnx9Y2pxrtPySOLTTDPdvshOT9eLGcf05NvHz7Npicp8oz3qRW8sii4zapKrqgsRQGFKDIopERZGS4MrspCYZyRImRGIeeSSbWSbEVplh2S11vvtXe_BhN63dahNE2DnXFD0DKqgCn1XxBkXgjJR_BoC5beheCN1de-btFvNFA9BqPvg4nsi510WLWm2pO7JCLwagdgKLGxHruyDnuOS-CKQ-TSLVeH3tze99FfaSEzmevl9zOtThUc_zhlWkT-5Za36DRe-Og8P2MUOKUAMv6H_ctYBr12g-9iDP8Y4Q8zbrfM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17586749</pqid></control><display><type>article</type><title>Evaluation of Extraction Methodologies for Corn Kernel (Zea mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA</title><source>American Chemical Society</source><source>MEDLINE</source><creator>Holden, Marcia J. ; Blasic, Joseph R. ; Bussjaeger, Louis ; Kao, Chuan ; Shokere, Luke A. ; Kendall, Donald C. ; Freese, Larry ; Jenkins, G. Ronald</creator><creatorcontrib>Holden, Marcia J. ; Blasic, Joseph R. ; Bussjaeger, Louis ; Kao, Chuan ; Shokere, Luke A. ; Kendall, Donald C. ; Freese, Larry ; Jenkins, G. Ronald</creatorcontrib><description>Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were evaluated for extracting DNA from ground corn kernels spiked with 0.1% (m/m) CBH351 (StarLink) corn. DNA preparations were evaluated for purity and fragment size. Extraction efficiency was determined. The alcohol dehydrogenase gene (adh1) and the CBH351 (cry9C, 35S promoter) genes in the genomic DNA were detected using PCR. DNA isolated by two of the methods proved unsuitable for performing PCR amplification. All other methods produced some DNA preparations that gave false negative PCR results. We observed that cornstarch, a primary component of corn kernels, was not an inhibitor of PCR, while acidic polysaccharides were. Our data suggest that amplification of an endogenous positive control gene, as an indicator for the absence of PCR inhibitors, is not always valid. This study points out aspects of DNA isolation that need to be considered when choosing a method for a particular plant/tissue type. Keywords: Zea mays; corn; genomic; DNA; extraction; polymerase chain reaction; PCR; PCR inhibition</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf0211130</identifier><identifier>PMID: 12696922</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>alcohol dehydrogenase ; Biological and medical sciences ; Biotechnology ; corn ; DNA ; DNA, Plant - isolation & purification ; False Negative Reactions ; Food industries ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; General aspects ; genes ; Methods of analysis, processing and quality control, regulation, standards ; Particle Size ; Plants, Genetically Modified - genetics ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; seeds ; Zea mays ; Zea mays - genetics</subject><ispartof>Journal of agricultural and food chemistry, 2003-04, Vol.51 (9), p.2468-2474</ispartof><rights>Copyright © 2003 American Chemical Society</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a434t-f64dfa64f3dd7b07c68186831877a7de46eabc89a0850a13e9a447279b72b0033</citedby><cites>FETCH-LOGICAL-a434t-f64dfa64f3dd7b07c68186831877a7de46eabc89a0850a13e9a447279b72b0033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf0211130$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf0211130$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14714941$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12696922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Holden, Marcia J.</creatorcontrib><creatorcontrib>Blasic, Joseph R.</creatorcontrib><creatorcontrib>Bussjaeger, Louis</creatorcontrib><creatorcontrib>Kao, Chuan</creatorcontrib><creatorcontrib>Shokere, Luke A.</creatorcontrib><creatorcontrib>Kendall, Donald C.</creatorcontrib><creatorcontrib>Freese, Larry</creatorcontrib><creatorcontrib>Jenkins, G. Ronald</creatorcontrib><title>Evaluation of Extraction Methodologies for Corn Kernel (Zea mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were evaluated for extracting DNA from ground corn kernels spiked with 0.1% (m/m) CBH351 (StarLink) corn. DNA preparations were evaluated for purity and fragment size. Extraction efficiency was determined. The alcohol dehydrogenase gene (adh1) and the CBH351 (cry9C, 35S promoter) genes in the genomic DNA were detected using PCR. DNA isolated by two of the methods proved unsuitable for performing PCR amplification. All other methods produced some DNA preparations that gave false negative PCR results. We observed that cornstarch, a primary component of corn kernels, was not an inhibitor of PCR, while acidic polysaccharides were. Our data suggest that amplification of an endogenous positive control gene, as an indicator for the absence of PCR inhibitors, is not always valid. This study points out aspects of DNA isolation that need to be considered when choosing a method for a particular plant/tissue type. Keywords: Zea mays; corn; genomic; DNA; extraction; polymerase chain reaction; PCR; PCR inhibition</description><subject>alcohol dehydrogenase</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>corn</subject><subject>DNA</subject><subject>DNA, Plant - isolation & purification</subject><subject>False Negative Reactions</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>General aspects</subject><subject>genes</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>Particle Size</subject><subject>Plants, Genetically Modified - genetics</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>seeds</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9v0zAUxy0EYmVw4B-AXEDsEPBzHDs-lrYDxPgx1l24WK-JvaVL4mEn03rkP8dZy3pB4mLL7338sZ6-JuQ50LdAGbxb27gCZPQBmUDOaJoDFA_JhMZyWuQCDsiTENaU0iKX9DE5ACaUUIxNyO_FDTYD9rXrEmeTxW3vsbw7fTH9patc4y5qExLrfDJzvks-G9-ZJnnz02DS4iYcJfOv07v23PSm_CtaRo1Jpq0buj6Mhfe1i-3LbhRu0rnx9Y2pxrtPySOLTTDPdvshOT9eLGcf05NvHz7Npicp8oz3qRW8sii4zapKrqgsRQGFKDIopERZGS4MrspCYZyRImRGIeeSSbWSbEVplh2S11vvtXe_BhN63dahNE2DnXFD0DKqgCn1XxBkXgjJR_BoC5beheCN1de-btFvNFA9BqPvg4nsi510WLWm2pO7JCLwagdgKLGxHruyDnuOS-CKQ-TSLVeH3tze99FfaSEzmevl9zOtThUc_zhlWkT-5Za36DRe-Og8P2MUOKUAMv6H_ctYBr12g-9iDP8Y4Q8zbrfM</recordid><startdate>20030423</startdate><enddate>20030423</enddate><creator>Holden, Marcia J.</creator><creator>Blasic, Joseph R.</creator><creator>Bussjaeger, Louis</creator><creator>Kao, Chuan</creator><creator>Shokere, Luke A.</creator><creator>Kendall, Donald C.</creator><creator>Freese, Larry</creator><creator>Jenkins, G. Ronald</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030423</creationdate><title>Evaluation of Extraction Methodologies for Corn Kernel (Zea mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA</title><author>Holden, Marcia J. ; Blasic, Joseph R. ; Bussjaeger, Louis ; Kao, Chuan ; Shokere, Luke A. ; Kendall, Donald C. ; Freese, Larry ; Jenkins, G. Ronald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a434t-f64dfa64f3dd7b07c68186831877a7de46eabc89a0850a13e9a447279b72b0033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>alcohol dehydrogenase</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>corn</topic><topic>DNA</topic><topic>DNA, Plant - isolation & purification</topic><topic>False Negative Reactions</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>General aspects</topic><topic>genes</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>Particle Size</topic><topic>Plants, Genetically Modified - genetics</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>seeds</topic><topic>Zea mays</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holden, Marcia J.</creatorcontrib><creatorcontrib>Blasic, Joseph R.</creatorcontrib><creatorcontrib>Bussjaeger, Louis</creatorcontrib><creatorcontrib>Kao, Chuan</creatorcontrib><creatorcontrib>Shokere, Luke A.</creatorcontrib><creatorcontrib>Kendall, Donald C.</creatorcontrib><creatorcontrib>Freese, Larry</creatorcontrib><creatorcontrib>Jenkins, G. Ronald</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holden, Marcia J.</au><au>Blasic, Joseph R.</au><au>Bussjaeger, Louis</au><au>Kao, Chuan</au><au>Shokere, Luke A.</au><au>Kendall, Donald C.</au><au>Freese, Larry</au><au>Jenkins, G. Ronald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Extraction Methodologies for Corn Kernel (Zea mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2003-04-23</date><risdate>2003</risdate><volume>51</volume><issue>9</issue><spage>2468</spage><epage>2474</epage><pages>2468-2474</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were evaluated for extracting DNA from ground corn kernels spiked with 0.1% (m/m) CBH351 (StarLink) corn. DNA preparations were evaluated for purity and fragment size. Extraction efficiency was determined. The alcohol dehydrogenase gene (adh1) and the CBH351 (cry9C, 35S promoter) genes in the genomic DNA were detected using PCR. DNA isolated by two of the methods proved unsuitable for performing PCR amplification. All other methods produced some DNA preparations that gave false negative PCR results. We observed that cornstarch, a primary component of corn kernels, was not an inhibitor of PCR, while acidic polysaccharides were. Our data suggest that amplification of an endogenous positive control gene, as an indicator for the absence of PCR inhibitors, is not always valid. This study points out aspects of DNA isolation that need to be considered when choosing a method for a particular plant/tissue type. Keywords: Zea mays; corn; genomic; DNA; extraction; polymerase chain reaction; PCR; PCR inhibition</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>12696922</pmid><doi>10.1021/jf0211130</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2003-04, Vol.51 (9), p.2468-2474 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73181299 |
| source | American Chemical Society; MEDLINE |
| subjects | alcohol dehydrogenase Biological and medical sciences Biotechnology corn DNA DNA, Plant - isolation & purification False Negative Reactions Food industries Fundamental and applied biological sciences. Psychology Gene Amplification General aspects genes Methods of analysis, processing and quality control, regulation, standards Particle Size Plants, Genetically Modified - genetics polymerase chain reaction Polymerase Chain Reaction - methods seeds Zea mays Zea mays - genetics |
| title | Evaluation of Extraction Methodologies for Corn Kernel (Zea mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T23%3A13%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20Extraction%20Methodologies%20for%20Corn%20Kernel%20(Zea%20mays)%20DNA%20for%20Detection%20of%20Trace%20Amounts%20of%20Biotechnology-Derived%20DNA&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Holden,%20Marcia%20J.&rft.date=2003-04-23&rft.volume=51&rft.issue=9&rft.spage=2468&rft.epage=2474&rft.pages=2468-2474&rft.issn=0021-8561&rft.eissn=1520-5118&rft.coden=JAFCAU&rft_id=info:doi/10.1021/jf0211130&rft_dat=%3Cproquest_cross%3E17586749%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17586749&rft_id=info:pmid/12696922&rfr_iscdi=true |