Highly Conserved Neisseria meningitidis Inner-Core Lipopolysaccharide Epitope Confers Protection against Experimental Meningococcal Bacteremia
Inner-core lipopolysaccharide (LPS) from Neisseria meningitidis is under investigation as a vaccine for prevention of meningococcal disease caused by N. meningitidis serogroup B (NmB). We investigated the functional activity of murine monoclonal antibody (MAb) B5 that recognizes a highly conserved (...
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| Veröffentlicht in: | The Journal of infectious diseases 2003-04, Vol.187 (8), p.1223-1234 |
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| container_title | The Journal of infectious diseases |
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| creator | Plested, Joyce S. Harris, Shannon L. Wright, J. Claire Coull, Philip A. Makepeace, Katherine Gidney, Margaret-Anne J. Brisson, Jean-Robert Richards, James C. Granoff, Dan M. Moxon, E. Richard |
| description | Inner-core lipopolysaccharide (LPS) from Neisseria meningitidis is under investigation as a vaccine for prevention of meningococcal disease caused by N. meningitidis serogroup B (NmB). We investigated the functional activity of murine monoclonal antibody (MAb) B5 that recognizes a highly conserved (galE) LPS epitope. Three patterns of MAb reactivity were observed in N. meningitidis by Western blot, depending on the relative prevalence of sialylated, nonsialylated, and/or truncated LPS glycoforms. Three representative N. meningitidis strains (8047, M986, and 2996) were investigated with MAb B5 in functional assays in vitro and in vivo. MAb B5 completely protected infant rats against bacteremia caused by 8047, partially protected against 2996, and had no protective activity against M986. Thus, an inner-core LPS epitope can be a target for protective immunity, but the affinity of MAb B5 may only be sufficient to mediate protection against NmB strains possessing at least some truncated glycoforms |
| doi_str_mv | 10.1086/368360 |
| format | Article |
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Three representative N. meningitidis strains (8047, M986, and 2996) were investigated with MAb B5 in functional assays in vitro and in vivo. MAb B5 completely protected infant rats against bacteremia caused by 8047, partially protected against 2996, and had no protective activity against M986. Thus, an inner-core LPS epitope can be a target for protective immunity, but the affinity of MAb B5 may only be sufficient to mediate protection against NmB strains possessing at least some truncated glycoforms</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/368360</identifier><identifier>PMID: 12696001</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Animals ; Animals, Suckling ; Antibodies, Bacterial - immunology ; Antibodies, Monoclonal - immunology ; Bacteremia ; Bacteremia - immunology ; Bacteremia - prevention & control ; Bacteria ; Bacterial diseases ; Bacterial sepsis ; Bacterial Vaccines - immunology ; Biological and medical sciences ; Conserved Sequence - immunology ; Epitopes ; Epitopes - chemistry ; Epitopes - genetics ; Epitopes - immunology ; Gels ; Hepatitis A ; Human bacterial diseases ; Immunization, Passive ; Infectious diseases ; Lipopolysaccharides ; Lipopolysaccharides - chemistry ; Lipopolysaccharides - immunology ; Medical sciences ; Meningococcal Infections - immunology ; Meningococcal Infections - prevention & control ; Mice ; Models, Molecular ; Monoclonal antibodies ; Neisseria meningitidis ; Neisseria meningitidis - chemistry ; Neisseria meningitidis - classification ; Neisseria meningitidis - genetics ; Neisseria meningitidis - immunology ; Phylogeny ; Polysaccharides ; Rats ; Rats, Wistar ; Reactivity ; Vaccination</subject><ispartof>The Journal of infectious diseases, 2003-04, Vol.187 (8), p.1223-1234</ispartof><rights>Copyright 2003 Infectious Diseases Society of America</rights><rights>2003 by the Infectious Diseases Society of America 2003</rights><rights>2003 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Apr 15 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-402d2970e58629daead08ddf854a9801c0bb47e75633cd24bc7f82f504ccafc93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30085607$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30085607$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57996,58229</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14734375$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12696001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Plested, Joyce S.</creatorcontrib><creatorcontrib>Harris, Shannon L.</creatorcontrib><creatorcontrib>Wright, J. Claire</creatorcontrib><creatorcontrib>Coull, Philip A.</creatorcontrib><creatorcontrib>Makepeace, Katherine</creatorcontrib><creatorcontrib>Gidney, Margaret-Anne J.</creatorcontrib><creatorcontrib>Brisson, Jean-Robert</creatorcontrib><creatorcontrib>Richards, James C.</creatorcontrib><creatorcontrib>Granoff, Dan M.</creatorcontrib><creatorcontrib>Moxon, E. Richard</creatorcontrib><title>Highly Conserved Neisseria meningitidis Inner-Core Lipopolysaccharide Epitope Confers Protection against Experimental Meningococcal Bacteremia</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><addtitle>The Journal of Infectious Diseases</addtitle><description>Inner-core lipopolysaccharide (LPS) from Neisseria meningitidis is under investigation as a vaccine for prevention of meningococcal disease caused by N. meningitidis serogroup B (NmB). We investigated the functional activity of murine monoclonal antibody (MAb) B5 that recognizes a highly conserved (galE) LPS epitope. Three patterns of MAb reactivity were observed in N. meningitidis by Western blot, depending on the relative prevalence of sialylated, nonsialylated, and/or truncated LPS glycoforms. Three representative N. meningitidis strains (8047, M986, and 2996) were investigated with MAb B5 in functional assays in vitro and in vivo. MAb B5 completely protected infant rats against bacteremia caused by 8047, partially protected against 2996, and had no protective activity against M986. Thus, an inner-core LPS epitope can be a target for protective immunity, but the affinity of MAb B5 may only be sufficient to mediate protection against NmB strains possessing at least some truncated glycoforms</description><subject>Animals</subject><subject>Animals, Suckling</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Bacteremia</subject><subject>Bacteremia - immunology</subject><subject>Bacteremia - prevention & control</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Bacterial sepsis</subject><subject>Bacterial Vaccines - immunology</subject><subject>Biological and medical sciences</subject><subject>Conserved Sequence - immunology</subject><subject>Epitopes</subject><subject>Epitopes - chemistry</subject><subject>Epitopes - genetics</subject><subject>Epitopes - immunology</subject><subject>Gels</subject><subject>Hepatitis A</subject><subject>Human bacterial diseases</subject><subject>Immunization, Passive</subject><subject>Infectious diseases</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - chemistry</subject><subject>Lipopolysaccharides - immunology</subject><subject>Medical sciences</subject><subject>Meningococcal Infections - immunology</subject><subject>Meningococcal Infections - prevention & control</subject><subject>Mice</subject><subject>Models, Molecular</subject><subject>Monoclonal antibodies</subject><subject>Neisseria meningitidis</subject><subject>Neisseria meningitidis - chemistry</subject><subject>Neisseria meningitidis - classification</subject><subject>Neisseria meningitidis - genetics</subject><subject>Neisseria meningitidis - immunology</subject><subject>Phylogeny</subject><subject>Polysaccharides</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reactivity</subject><subject>Vaccination</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd9u0zAUhyMEYt2ANwCFSXAXOI4T_7mEqtCJMnYBYtpN5DonnUtqB9tB60vwzLi0WiUkrmzr9-k7x-dk2TMCbwgI9pYyQRk8yCakprxgjNCH2QSgLAsipDzJTkNYA0BFGX-cnZCSSQZAJtnvuVnd9tt86mxA_wvb_BJNSFej8g1aY1cmmtaE_MJa9MXUecwXZnCD67dBaX2rvGkxnw0mugF3mg59yK-8i6ijcTZXK2VsiPnsbkjW5Iyqzz__VTvttE6v90pH9Lgx6kn2qFN9wKeH8yz79mH2dTovFl8-XkzfLQpdERGLCsq2lBywFqyUrULVgmjbTtSVkgKIhuWy4shrRqluy2qpeSfKroYq1eu0pGfZ67138O7niCE2GxM09r2y6MbQcEoE0HoHnv8Drt3obeqtKUsqQVLBjjbtXQgeu2ZIP1V-2xBodutp9utJ4IuDbVxusD1ih30k4NUBUCFNpvPKahOOXMVpRXmduJd7zo3D_4s93zPrEJ2_pyiAqBnwlBf73ISId_e58j8axlORZn5901zK71efrm8gTeQPSOC8RA</recordid><startdate>20030415</startdate><enddate>20030415</enddate><creator>Plested, Joyce S.</creator><creator>Harris, Shannon L.</creator><creator>Wright, J. Claire</creator><creator>Coull, Philip A.</creator><creator>Makepeace, Katherine</creator><creator>Gidney, Margaret-Anne J.</creator><creator>Brisson, Jean-Robert</creator><creator>Richards, James C.</creator><creator>Granoff, Dan M.</creator><creator>Moxon, E. Richard</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20030415</creationdate><title>Highly Conserved Neisseria meningitidis Inner-Core Lipopolysaccharide Epitope Confers Protection against Experimental Meningococcal Bacteremia</title><author>Plested, Joyce S. ; Harris, Shannon L. ; Wright, J. Claire ; Coull, Philip A. ; Makepeace, Katherine ; Gidney, Margaret-Anne J. ; Brisson, Jean-Robert ; Richards, James C. ; Granoff, Dan M. ; Moxon, E. 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Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly Conserved Neisseria meningitidis Inner-Core Lipopolysaccharide Epitope Confers Protection against Experimental Meningococcal Bacteremia</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>The Journal of Infectious Diseases</stitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2003-04-15</date><risdate>2003</risdate><volume>187</volume><issue>8</issue><spage>1223</spage><epage>1234</epage><pages>1223-1234</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Inner-core lipopolysaccharide (LPS) from Neisseria meningitidis is under investigation as a vaccine for prevention of meningococcal disease caused by N. meningitidis serogroup B (NmB). We investigated the functional activity of murine monoclonal antibody (MAb) B5 that recognizes a highly conserved (galE) LPS epitope. Three patterns of MAb reactivity were observed in N. meningitidis by Western blot, depending on the relative prevalence of sialylated, nonsialylated, and/or truncated LPS glycoforms. Three representative N. meningitidis strains (8047, M986, and 2996) were investigated with MAb B5 in functional assays in vitro and in vivo. MAb B5 completely protected infant rats against bacteremia caused by 8047, partially protected against 2996, and had no protective activity against M986. Thus, an inner-core LPS epitope can be a target for protective immunity, but the affinity of MAb B5 may only be sufficient to mediate protection against NmB strains possessing at least some truncated glycoforms</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>12696001</pmid><doi>10.1086/368360</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Animals, Suckling Antibodies, Bacterial - immunology Antibodies, Monoclonal - immunology Bacteremia Bacteremia - immunology Bacteremia - prevention & control Bacteria Bacterial diseases Bacterial sepsis Bacterial Vaccines - immunology Biological and medical sciences Conserved Sequence - immunology Epitopes Epitopes - chemistry Epitopes - genetics Epitopes - immunology Gels Hepatitis A Human bacterial diseases Immunization, Passive Infectious diseases Lipopolysaccharides Lipopolysaccharides - chemistry Lipopolysaccharides - immunology Medical sciences Meningococcal Infections - immunology Meningococcal Infections - prevention & control Mice Models, Molecular Monoclonal antibodies Neisseria meningitidis Neisseria meningitidis - chemistry Neisseria meningitidis - classification Neisseria meningitidis - genetics Neisseria meningitidis - immunology Phylogeny Polysaccharides Rats Rats, Wistar Reactivity Vaccination |
| title | Highly Conserved Neisseria meningitidis Inner-Core Lipopolysaccharide Epitope Confers Protection against Experimental Meningococcal Bacteremia |
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