Expression of epidermal growth factor receptor and its relationship to apoptosis in myelodysplastic syndromes
To explore the expression of epidermal growth factor receptor (EGFR) in hematopoietic cells and investigate its relationship to apoptosis in myelodysplastic syndrome (MDS) patients. Mononuclear cells from 18 MDS patients were used to analyze the expression of EGFR and the apoptotic signals by immuno...
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| Veröffentlicht in: | Zhōnghuá xuèyèxué zázhì 2003-01, Vol.24 (1), p.22-24 |
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| container_title | Zhōnghuá xuèyèxué zázhì |
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| creator | Li, Xiao Pu, Quan Beckham, Cassandra Deeg, Joachim H |
| description | To explore the expression of epidermal growth factor receptor (EGFR) in hematopoietic cells and investigate its relationship to apoptosis in myelodysplastic syndrome (MDS) patients.
Mononuclear cells from 18 MDS patients were used to analyze the expression of EGFR and the apoptotic signals by immunocytochemical technique and TdT-mediated dUTP nick end labelling (TUNEL) fluorescein. Flow cytometry (FCM) sorted CD(34)(+) cells from 15 MDS cases and 6 normal donors were also analyzed for the EGFR expression and apoptotic signals. Nine normal marrow samples were taken as controls. EGFR expression and its relationship to apoptosis were analysed.
(1) There was a higher EGFR expression rate (38.6 +/- 24.6)% in nucleated cells of MDS marrow than in normal marrow (18.1 +/- 14.0)% (P < 0.05). (2) Apoptosis occurred much more in EGFR negative cells (16.1%) than in EGFR positive cells (1.4%) (P < 0.01). EGFR expression showed negative correlation with apoptosis (r = -0.701; t(r) = 3.60; P < 0.01). (3) In CD(34)(+) cells, |
| format | Article |
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Mononuclear cells from 18 MDS patients were used to analyze the expression of EGFR and the apoptotic signals by immunocytochemical technique and TdT-mediated dUTP nick end labelling (TUNEL) fluorescein. Flow cytometry (FCM) sorted CD(34)(+) cells from 15 MDS cases and 6 normal donors were also analyzed for the EGFR expression and apoptotic signals. Nine normal marrow samples were taken as controls. EGFR expression and its relationship to apoptosis were analysed.
(1) There was a higher EGFR expression rate (38.6 +/- 24.6)% in nucleated cells of MDS marrow than in normal marrow (18.1 +/- 14.0)% (P < 0.05). (2) Apoptosis occurred much more in EGFR negative cells (16.1%) than in EGFR positive cells (1.4%) (P < 0.01). EGFR expression showed negative correlation with apoptosis (r = -0.701; t(r) = 3.60; P < 0.01). (3) In CD(34)(+) cells,</description><identifier>ISSN: 0253-2727</identifier><identifier>PMID: 12679005</identifier><language>chi</language><publisher>China</publisher><subject>Adult ; Aged ; Apoptosis ; Bone Marrow Cells - metabolism ; Female ; Flow Cytometry ; Humans ; In Situ Nick-End Labeling ; Male ; Middle Aged ; Myelodysplastic Syndromes - metabolism ; Myelodysplastic Syndromes - pathology ; Receptor, Epidermal Growth Factor - biosynthesis</subject><ispartof>Zhōnghuá xuèyèxué zázhì, 2003-01, Vol.24 (1), p.22-24</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12679005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiao</creatorcontrib><creatorcontrib>Pu, Quan</creatorcontrib><creatorcontrib>Beckham, Cassandra</creatorcontrib><creatorcontrib>Deeg, Joachim H</creatorcontrib><title>Expression of epidermal growth factor receptor and its relationship to apoptosis in myelodysplastic syndromes</title><title>Zhōnghuá xuèyèxué zázhì</title><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><description>To explore the expression of epidermal growth factor receptor (EGFR) in hematopoietic cells and investigate its relationship to apoptosis in myelodysplastic syndrome (MDS) patients.
Mononuclear cells from 18 MDS patients were used to analyze the expression of EGFR and the apoptotic signals by immunocytochemical technique and TdT-mediated dUTP nick end labelling (TUNEL) fluorescein. Flow cytometry (FCM) sorted CD(34)(+) cells from 15 MDS cases and 6 normal donors were also analyzed for the EGFR expression and apoptotic signals. Nine normal marrow samples were taken as controls. EGFR expression and its relationship to apoptosis were analysed.
(1) There was a higher EGFR expression rate (38.6 +/- 24.6)% in nucleated cells of MDS marrow than in normal marrow (18.1 +/- 14.0)% (P < 0.05). (2) Apoptosis occurred much more in EGFR negative cells (16.1%) than in EGFR positive cells (1.4%) (P < 0.01). EGFR expression showed negative correlation with apoptosis (r = -0.701; t(r) = 3.60; P < 0.01). (3) In CD(34)(+) cells,</description><subject>Adult</subject><subject>Aged</subject><subject>Apoptosis</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myelodysplastic Syndromes - metabolism</subject><subject>Myelodysplastic Syndromes - pathology</subject><subject>Receptor, Epidermal Growth Factor - biosynthesis</subject><issn>0253-2727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1UMtKAzEAzEGxpfYXJCdvC3mne5RSH1Dwouclm4cNJJuYpOj-vSvW0wzzOswVWCPCaUckkSuwrdWPiGMqdpSiG7DCRMgeIb4G8fCdi138NMHkoM3e2BJVgB8lfbUTdEq3VGCx2uZfoiYDfauLEFRbSvXkM2wJqpwWv_oK_QTjbEMyc81B1eY1rPNkSoq23oJrp0K12wtuwPvj4W3_3B1fn172D8cuY8Jap7DmPSNaGi2RMXxUUkjGmHOE4x6PnGLpuFSMWKucE73RVDO8E5QRygSjG3D_t5tL-jzb2oboq7YhqMmmcx3kMtD3Ai3Bu0vwPEZrhlx8VGUe_g-iP3XhZGU</recordid><startdate>200301</startdate><enddate>200301</enddate><creator>Li, Xiao</creator><creator>Pu, Quan</creator><creator>Beckham, Cassandra</creator><creator>Deeg, Joachim H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200301</creationdate><title>Expression of epidermal growth factor receptor and its relationship to apoptosis in myelodysplastic syndromes</title><author>Li, Xiao ; Pu, Quan ; Beckham, Cassandra ; Deeg, Joachim H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-a1c5942c7dc70dd5ba767444ff25191b5317f57a42eeaff69dc3c418634234643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Apoptosis</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myelodysplastic Syndromes - metabolism</topic><topic>Myelodysplastic Syndromes - pathology</topic><topic>Receptor, Epidermal Growth Factor - biosynthesis</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiao</creatorcontrib><creatorcontrib>Pu, Quan</creatorcontrib><creatorcontrib>Beckham, Cassandra</creatorcontrib><creatorcontrib>Deeg, Joachim H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiao</au><au>Pu, Quan</au><au>Beckham, Cassandra</au><au>Deeg, Joachim H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of epidermal growth factor receptor and its relationship to apoptosis in myelodysplastic syndromes</atitle><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><date>2003-01</date><risdate>2003</risdate><volume>24</volume><issue>1</issue><spage>22</spage><epage>24</epage><pages>22-24</pages><issn>0253-2727</issn><abstract>To explore the expression of epidermal growth factor receptor (EGFR) in hematopoietic cells and investigate its relationship to apoptosis in myelodysplastic syndrome (MDS) patients.
Mononuclear cells from 18 MDS patients were used to analyze the expression of EGFR and the apoptotic signals by immunocytochemical technique and TdT-mediated dUTP nick end labelling (TUNEL) fluorescein. Flow cytometry (FCM) sorted CD(34)(+) cells from 15 MDS cases and 6 normal donors were also analyzed for the EGFR expression and apoptotic signals. Nine normal marrow samples were taken as controls. EGFR expression and its relationship to apoptosis were analysed.
(1) There was a higher EGFR expression rate (38.6 +/- 24.6)% in nucleated cells of MDS marrow than in normal marrow (18.1 +/- 14.0)% (P < 0.05). (2) Apoptosis occurred much more in EGFR negative cells (16.1%) than in EGFR positive cells (1.4%) (P < 0.01). EGFR expression showed negative correlation with apoptosis (r = -0.701; t(r) = 3.60; P < 0.01). (3) In CD(34)(+) cells,</abstract><cop>China</cop><pmid>12679005</pmid><tpages>3</tpages></addata></record> |
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| ispartof | Zhōnghuá xuèyèxué zázhì, 2003-01, Vol.24 (1), p.22-24 |
| issn | 0253-2727 |
| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Aged Apoptosis Bone Marrow Cells - metabolism Female Flow Cytometry Humans In Situ Nick-End Labeling Male Middle Aged Myelodysplastic Syndromes - metabolism Myelodysplastic Syndromes - pathology Receptor, Epidermal Growth Factor - biosynthesis |
| title | Expression of epidermal growth factor receptor and its relationship to apoptosis in myelodysplastic syndromes |
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