A novel cDNA encodes a putative hRALY-like protein, hRALYL
High throughput cDNA sequencing and 5'-rapid amplification of cDNA ends (5'RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF e...
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| Veröffentlicht in: | Molecular biology reports 2003-03, Vol.30 (1), p.61-67 |
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| container_title | Molecular biology reports |
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| creator | Ji, Chao-Neng Chen, Jin-Zhong Xie, Yi Wang, Shu Qian, Ji Zhao, Enpeng Jin, Wei Wu, Xiao-Zhou Xu, Wan-Xiang Ying, Kang Mao, Yu-Min |
| description | High throughput cDNA sequencing and 5'-rapid amplification of cDNA ends (5'RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.2. hRALYL might be a member of hnRNPC subfamily. |
| doi_str_mv | 10.1023/A:1022295311177 |
| format | Article |
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Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. 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| subjects | Amino Acid Sequence Base Sequence DNA, Complementary Genes Heterogeneous-Nuclear Ribonucleoprotein Group C - genetics Heterogeneous-Nuclear Ribonucleoproteins - genetics Humans Molecular Sequence Data |
| title | A novel cDNA encodes a putative hRALY-like protein, hRALYL |
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