Substance P inhibits carbamylcholine-stimulated 22Na + efflux from acetylcholine receptor-enriched Torpedo electroplaque membrane vesicles
The effect of substance P on nicotinic acetylcholine receptor function was examined in Torpedo electroplaque membranes. The peptide inhibited carbamylcholine-stimulated 22Na + efflux in a concentration-dependent manner. By irreversibly blocking spare receptors with α-bungarotoxin, the IC 50 for subs...
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| Veröffentlicht in: | Brain research 1992-07, Vol.586 (2), p.348-351 |
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| container_end_page | 351 |
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| container_title | Brain research |
| container_volume | 586 |
| creator | Min, Churl K. Weiland, Gregory A. |
| description | The effect of substance P on nicotinic acetylcholine receptor function was examined in
Torpedo electroplaque membranes. The peptide inhibited carbamylcholine-stimulated
22Na
+ efflux in a concentration-dependent manner. By irreversibly blocking spare receptors with α-bungarotoxin, the IC
50 for substance P was shown to be less than 3 μM. Inhibition by substance P was slow relative to receptor activation by carbamylcholine, consistent with an enhancement of desensitization or a slow allosteric block. |
| doi_str_mv | 10.1016/0006-8993(92)91647-W |
| format | Article |
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Torpedo electroplaque membranes. The peptide inhibited carbamylcholine-stimulated
22Na
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50 for substance P was shown to be less than 3 μM. Inhibition by substance P was slow relative to receptor activation by carbamylcholine, consistent with an enhancement of desensitization or a slow allosteric block.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(92)91647-W</identifier><identifier>PMID: 1381656</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>22Na + flux ; Allosteric block ; Animals ; Biological and medical sciences ; Bungarotoxins - metabolism ; Carbachol - administration & dosage ; Carbachol - pharmacology ; Carbamylcoline ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Desensitization ; Electric Organ - drug effects ; Electric Organ - metabolism ; Fundamental and applied biological sciences. Psychology ; Kinetics ; Membrane vesicle ; Neuromodulation ; Nicotinic acetylcholine receptor ; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ ; Receptors, Nicotinic - drug effects ; Receptors, Nicotinic - metabolism ; Receptors, Nicotinic - physiology ; Sodium - metabolism ; Sodium Radioisotopes ; Substance P ; Substance P - pharmacology ; Torpedo ; Torpedo electroplaque ; Vertebrates: nervous system and sense organs ; α-Bungarotoxin</subject><ispartof>Brain research, 1992-07, Vol.586 (2), p.348-351</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c279w-12d971db8f5c360bcb4ff16daf83a4598c13bf574dfc6584d9d7eb64e852ea13</citedby><cites>FETCH-LOGICAL-c279w-12d971db8f5c360bcb4ff16daf83a4598c13bf574dfc6584d9d7eb64e852ea13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-8993(92)91647-W$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5458755$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1381656$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Min, Churl K.</creatorcontrib><creatorcontrib>Weiland, Gregory A.</creatorcontrib><title>Substance P inhibits carbamylcholine-stimulated 22Na + efflux from acetylcholine receptor-enriched Torpedo electroplaque membrane vesicles</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The effect of substance P on nicotinic acetylcholine receptor function was examined in
Torpedo electroplaque membranes. The peptide inhibited carbamylcholine-stimulated
22Na
+ efflux in a concentration-dependent manner. By irreversibly blocking spare receptors with α-bungarotoxin, the IC
50 for substance P was shown to be less than 3 μM. Inhibition by substance P was slow relative to receptor activation by carbamylcholine, consistent with an enhancement of desensitization or a slow allosteric block.</description><subject>22Na + flux</subject><subject>Allosteric block</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bungarotoxins - metabolism</subject><subject>Carbachol - administration & dosage</subject><subject>Carbachol - pharmacology</subject><subject>Carbamylcoline</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Desensitization</subject><subject>Electric Organ - drug effects</subject><subject>Electric Organ - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kinetics</subject><subject>Membrane vesicle</subject><subject>Neuromodulation</subject><subject>Nicotinic acetylcholine receptor</subject><subject>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</subject><subject>Receptors, Nicotinic - drug effects</subject><subject>Receptors, Nicotinic - metabolism</subject><subject>Receptors, Nicotinic - physiology</subject><subject>Sodium - metabolism</subject><subject>Sodium Radioisotopes</subject><subject>Substance P</subject><subject>Substance P - pharmacology</subject><subject>Torpedo</subject><subject>Torpedo electroplaque</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>α-Bungarotoxin</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV1rFDEUhoModVv9Bwq5EKnIaJKZZCY3Qil-FIoKLvQy5OOEjWQ2YzJT27_grzbrLutdr0I4z3s4PC9CLyh5RwkV7wkhohmkbM8leyOp6Prm5hFa0aFnjWAdeYxWR-QpOi3lZ_22rSQn6IS2AxVcrNCfH4sps95awN9x2G6CCXPBVmejx_toNymGLTRlDuMS9QwOM_ZV47cYvI_LHfY5jVhbmI8szmBhmlNuYJuD3dTIOuUJXMIQwc45TVH_WgCPMJqsa-AWSrARyjP0xOtY4PnhPUPrTx_Xl1-a62-fry4vrhvLevm7oczJnjozeG5bQYw1nfdUOO2HVndcDpa2xvO-c94KPnROuh6M6GDgDDRtz9Dr_dopp3pHmdUYioUY6y1pKapvaU8lYRXs9qDNqZQMXk05jDrfK0rUrgG106t2epVk6l8D6qbGXh72L2YE9z-0V17nrw5zXayOvjqwoRwx3vGh57xiH_YYVBW3AbIqNkDtyYVqeFYuhYfv-Au1baYL</recordid><startdate>19920724</startdate><enddate>19920724</enddate><creator>Min, Churl K.</creator><creator>Weiland, Gregory A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920724</creationdate><title>Substance P inhibits carbamylcholine-stimulated 22Na + efflux from acetylcholine receptor-enriched Torpedo electroplaque membrane vesicles</title><author>Min, Churl K. ; Weiland, Gregory A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c279w-12d971db8f5c360bcb4ff16daf83a4598c13bf574dfc6584d9d7eb64e852ea13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>22Na + flux</topic><topic>Allosteric block</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bungarotoxins - metabolism</topic><topic>Carbachol - administration & dosage</topic><topic>Carbachol - pharmacology</topic><topic>Carbamylcoline</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Desensitization</topic><topic>Electric Organ - drug effects</topic><topic>Electric Organ - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Kinetics</topic><topic>Membrane vesicle</topic><topic>Neuromodulation</topic><topic>Nicotinic acetylcholine receptor</topic><topic>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</topic><topic>Receptors, Nicotinic - drug effects</topic><topic>Receptors, Nicotinic - metabolism</topic><topic>Receptors, Nicotinic - physiology</topic><topic>Sodium - metabolism</topic><topic>Sodium Radioisotopes</topic><topic>Substance P</topic><topic>Substance P - pharmacology</topic><topic>Torpedo</topic><topic>Torpedo electroplaque</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>α-Bungarotoxin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Min, Churl K.</creatorcontrib><creatorcontrib>Weiland, Gregory A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Min, Churl K.</au><au>Weiland, Gregory A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Substance P inhibits carbamylcholine-stimulated 22Na + efflux from acetylcholine receptor-enriched Torpedo electroplaque membrane vesicles</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1992-07-24</date><risdate>1992</risdate><volume>586</volume><issue>2</issue><spage>348</spage><epage>351</epage><pages>348-351</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The effect of substance P on nicotinic acetylcholine receptor function was examined in
Torpedo electroplaque membranes. The peptide inhibited carbamylcholine-stimulated
22Na
+ efflux in a concentration-dependent manner. By irreversibly blocking spare receptors with α-bungarotoxin, the IC
50 for substance P was shown to be less than 3 μM. Inhibition by substance P was slow relative to receptor activation by carbamylcholine, consistent with an enhancement of desensitization or a slow allosteric block.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>1381656</pmid><doi>10.1016/0006-8993(92)91647-W</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Brain research, 1992-07, Vol.586 (2), p.348-351 |
| issn | 0006-8993 1872-6240 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 22Na + flux Allosteric block Animals Biological and medical sciences Bungarotoxins - metabolism Carbachol - administration & dosage Carbachol - pharmacology Carbamylcoline Cell Membrane - drug effects Cell Membrane - metabolism Desensitization Electric Organ - drug effects Electric Organ - metabolism Fundamental and applied biological sciences. Psychology Kinetics Membrane vesicle Neuromodulation Nicotinic acetylcholine receptor Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ Receptors, Nicotinic - drug effects Receptors, Nicotinic - metabolism Receptors, Nicotinic - physiology Sodium - metabolism Sodium Radioisotopes Substance P Substance P - pharmacology Torpedo Torpedo electroplaque Vertebrates: nervous system and sense organs α-Bungarotoxin |
| title | Substance P inhibits carbamylcholine-stimulated 22Na + efflux from acetylcholine receptor-enriched Torpedo electroplaque membrane vesicles |
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