Construction of a sequenced Clostridium perfringens-Escherichia coli shuttle plasmid
A new Clostridium perfringens-Escherichia coli shuttle plasmid has been constructed and its complete DNA sequence compiled. The vector, pJIR418, contains the replication regions from the C. perfringens replicon pIP404 and the E. coli vector pUC18. The multiple cloning site and lacZ gene from pUC18 a...
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| Veröffentlicht in: | Plasmid 1992-05, Vol.27 (3), p.207-219 |
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| creator | Sloan, Joan Warner, Tracy A. Scott, Paul T. Bannam, Trudi L. Berryman, David I. Rood, Julian I. |
| description | A new
Clostridium perfringens-Escherichia coli shuttle plasmid has been constructed and its complete DNA sequence compiled. The vector, pJIR418, contains the replication regions from the
C. perfringens replicon pIP404 and the
E. coli vector pUC18. The multiple cloning site and
lacZ gene from pUC18 are also present, which means that X-gal screening can be used to select recombinants in
E. coli. Both chloramphenicol and erythromycin resistance can be selected in
C. perfringens and
E. coli since pJIR418 carries the
C. perfringens catP and
ermBP genes. Insertional inactivation of either the
catP or
ermBP genes can also be used to directly screen recombinants in both organisms. The versatility of pJIR418 and its applicability for the cloning of toxin genes from
C. perfringens have been demonstrated by the manipulation of a cloned gene encoding the production of phospholipase C. |
| doi_str_mv | 10.1016/0147-619X(92)90023-4 |
| format | Article |
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Clostridium perfringens-Escherichia coli shuttle plasmid has been constructed and its complete DNA sequence compiled. The vector, pJIR418, contains the replication regions from the
C. perfringens replicon pIP404 and the
E. coli vector pUC18. The multiple cloning site and
lacZ gene from pUC18 are also present, which means that X-gal screening can be used to select recombinants in
E. coli. Both chloramphenicol and erythromycin resistance can be selected in
C. perfringens and
E. coli since pJIR418 carries the
C. perfringens catP and
ermBP genes. Insertional inactivation of either the
catP or
ermBP genes can also be used to directly screen recombinants in both organisms. The versatility of pJIR418 and its applicability for the cloning of toxin genes from
C. perfringens have been demonstrated by the manipulation of a cloned gene encoding the production of phospholipase C.</description><identifier>ISSN: 0147-619X</identifier><identifier>EISSN: 1095-9890</identifier><identifier>DOI: 10.1016/0147-619X(92)90023-4</identifier><identifier>PMID: 1513878</identifier><identifier>CODEN: PLSMDX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Bacterial Proteins - genetics ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Chloramphenicol Resistance - genetics ; Cloning, Molecular - methods ; Clostridium perfringens ; Clostridium perfringens - genetics ; Drug Resistance, Microbial - genetics ; Erythromycin - pharmacology ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Genetic Vectors ; Methods. Procedures. Technologies ; Molecular Sequence Data ; Plasmids ; Tetracycline Resistance - genetics ; Type C Phospholipases - genetics ; Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><ispartof>Plasmid, 1992-05, Vol.27 (3), p.207-219</ispartof><rights>1992 Academic Press, Inc. All rights of reproduction in any form reserved.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0147-619X(92)90023-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5304342$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1513878$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sloan, Joan</creatorcontrib><creatorcontrib>Warner, Tracy A.</creatorcontrib><creatorcontrib>Scott, Paul T.</creatorcontrib><creatorcontrib>Bannam, Trudi L.</creatorcontrib><creatorcontrib>Berryman, David I.</creatorcontrib><creatorcontrib>Rood, Julian I.</creatorcontrib><title>Construction of a sequenced Clostridium perfringens-Escherichia coli shuttle plasmid</title><title>Plasmid</title><addtitle>Plasmid</addtitle><description>A new
Clostridium perfringens-Escherichia coli shuttle plasmid has been constructed and its complete DNA sequence compiled. The vector, pJIR418, contains the replication regions from the
C. perfringens replicon pIP404 and the
E. coli vector pUC18. The multiple cloning site and
lacZ gene from pUC18 are also present, which means that X-gal screening can be used to select recombinants in
E. coli. Both chloramphenicol and erythromycin resistance can be selected in
C. perfringens and
E. coli since pJIR418 carries the
C. perfringens catP and
ermBP genes. Insertional inactivation of either the
catP or
ermBP genes can also be used to directly screen recombinants in both organisms. The versatility of pJIR418 and its applicability for the cloning of toxin genes from
C. perfringens have been demonstrated by the manipulation of a cloned gene encoding the production of phospholipase C.</description><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chloramphenicol Resistance - genetics</subject><subject>Cloning, Molecular - methods</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens - genetics</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Erythromycin - pharmacology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Tetracycline Resistance - genetics</subject><subject>Type C Phospholipases - genetics</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><issn>0147-619X</issn><issn>1095-9890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLAzEUhYMotVb_gcIsRHQxmpvMI9kIMtQHFNxUcBcymVsbmUdNZgT_vZlaunV1L5yPcx-HkHOgt0Ahu6OQ5HEG8v1ashtJKeNxckCmQGUaSyHpIZnukWNy4v0npTRjkE3IBFLgIhdTsiy61vduML3t2qhbRTry-DVga7CKiroLmq3s0EQbdCtn2w9sfTz3Zo3OmrXVkelqG_n10Pc1Rpta-8ZWp-RopWuPZ7s6I2-P82XxHC9en16Kh0WMLOV9rCljKBgiLyXKUlDKpQHJEp2BSUrKcmZEpiGvMGdMCKYh9KmQOZZBS_mMXP35blwXdva9aqw3WNe6xW7wKueQB0_2LwhZMM0gCeDFDhzKBiu1cbbR7kft_hX0y52uvdH1yunWWL_HUk4Tnozz7v8wDNd_W3TKG7v9qXVoelV1VgFVY4xqzEiNGSnJ1DZGlfBf0EmN4A</recordid><startdate>19920501</startdate><enddate>19920501</enddate><creator>Sloan, Joan</creator><creator>Warner, Tracy A.</creator><creator>Scott, Paul T.</creator><creator>Bannam, Trudi L.</creator><creator>Berryman, David I.</creator><creator>Rood, Julian I.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19920501</creationdate><title>Construction of a sequenced Clostridium perfringens-Escherichia coli shuttle plasmid</title><author>Sloan, Joan ; Warner, Tracy A. ; Scott, Paul T. ; Bannam, Trudi L. ; Berryman, David I. ; Rood, Julian I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e253t-a022e82ee3b9e9b80039c1924a61c4b0272c86a17de722882a117d5897eb27253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chloramphenicol Resistance - genetics</topic><topic>Cloning, Molecular - methods</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - genetics</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Erythromycin - pharmacology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Tetracycline Resistance - genetics</topic><topic>Type C Phospholipases - genetics</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sloan, Joan</creatorcontrib><creatorcontrib>Warner, Tracy A.</creatorcontrib><creatorcontrib>Scott, Paul T.</creatorcontrib><creatorcontrib>Bannam, Trudi L.</creatorcontrib><creatorcontrib>Berryman, David I.</creatorcontrib><creatorcontrib>Rood, Julian I.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Plasmid</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sloan, Joan</au><au>Warner, Tracy A.</au><au>Scott, Paul T.</au><au>Bannam, Trudi L.</au><au>Berryman, David I.</au><au>Rood, Julian I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of a sequenced Clostridium perfringens-Escherichia coli shuttle plasmid</atitle><jtitle>Plasmid</jtitle><addtitle>Plasmid</addtitle><date>1992-05-01</date><risdate>1992</risdate><volume>27</volume><issue>3</issue><spage>207</spage><epage>219</epage><pages>207-219</pages><issn>0147-619X</issn><eissn>1095-9890</eissn><coden>PLSMDX</coden><abstract>A new
Clostridium perfringens-Escherichia coli shuttle plasmid has been constructed and its complete DNA sequence compiled. The vector, pJIR418, contains the replication regions from the
C. perfringens replicon pIP404 and the
E. coli vector pUC18. The multiple cloning site and
lacZ gene from pUC18 are also present, which means that X-gal screening can be used to select recombinants in
E. coli. Both chloramphenicol and erythromycin resistance can be selected in
C. perfringens and
E. coli since pJIR418 carries the
C. perfringens catP and
ermBP genes. Insertional inactivation of either the
catP or
ermBP genes can also be used to directly screen recombinants in both organisms. The versatility of pJIR418 and its applicability for the cloning of toxin genes from
C. perfringens have been demonstrated by the manipulation of a cloned gene encoding the production of phospholipase C.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1513878</pmid><doi>10.1016/0147-619X(92)90023-4</doi><tpages>13</tpages></addata></record> |
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| ispartof | Plasmid, 1992-05, Vol.27 (3), p.207-219 |
| issn | 0147-619X 1095-9890 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacterial Proteins - genetics Base Sequence Biological and medical sciences Biotechnology Chloramphenicol Resistance - genetics Cloning, Molecular - methods Clostridium perfringens Clostridium perfringens - genetics Drug Resistance, Microbial - genetics Erythromycin - pharmacology Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Molecular Sequence Data Plasmids Tetracycline Resistance - genetics Type C Phospholipases - genetics Vectors (cloning, transfer, expression). Insertion sequences and transposons |
| title | Construction of a sequenced Clostridium perfringens-Escherichia coli shuttle plasmid |
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