Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)
A bacterial artificial chromosome (BAC) library for banana was constructed from leaves of the wild diploid 'Calcutta 4' clone (Musa acuminata subsp. Burmannicoides 2n = 2 x = 22). 'Calcutta 4' is widely used in breeding programs for its resistance to the current major disease of...
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| Veröffentlicht in: | Theoretical and applied genetics 2003-04, Vol.106 (6), p.1102-1106 |
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| creator | VILARINHOS, A. D PIFFANELLI, P LAGODA, P THIBIVILLIERS, S SABAU, X CARREEL, F D'HONT, A |
| description | A bacterial artificial chromosome (BAC) library for banana was constructed from leaves of the wild diploid 'Calcutta 4' clone (Musa acuminata subsp. Burmannicoides 2n = 2 x = 22). 'Calcutta 4' is widely used in breeding programs for its resistance to the current major disease of banana and is being used to build a genetic reference map of banana. As banana leaves are particularly rich in polyphenols and polysaccharides a protocol was adapted to isolate intact nuclei and high-molecular-weight (HMW) DNA. A total of 55,152 clones with an average insert size of 100 kb were picked. The frequency of BAC clones carrying inserts derived from chloroplast and mitochondrial DNA was estimated to be 1.5%. The coverage of the library is equivalent to 9.0-times the haploid genome. The BAC library was screened with 13 RFLP probes belonging to the 8 linkage groups of the consensus molecular map of banana. A total of 135 clones were identified giving an average of 10.38 clones for each locus. This BAC library will be a valuable starting tool for many of the goals of the recently emerged International Musa Genomic Consortium. One of our initial objectives will be to develop a banana physical map by BAC-FISH (fluorescent in situ hybridization) viewing the characterization of translocation break points. |
| doi_str_mv | 10.1007/s00122-002-1155-2 |
| format | Article |
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D ; PIFFANELLI, P ; LAGODA, P ; THIBIVILLIERS, S ; SABAU, X ; CARREEL, F ; D'HONT, A</creator><creatorcontrib>VILARINHOS, A. D ; PIFFANELLI, P ; LAGODA, P ; THIBIVILLIERS, S ; SABAU, X ; CARREEL, F ; D'HONT, A</creatorcontrib><description>A bacterial artificial chromosome (BAC) library for banana was constructed from leaves of the wild diploid 'Calcutta 4' clone (Musa acuminata subsp. Burmannicoides 2n = 2 x = 22). 'Calcutta 4' is widely used in breeding programs for its resistance to the current major disease of banana and is being used to build a genetic reference map of banana. As banana leaves are particularly rich in polyphenols and polysaccharides a protocol was adapted to isolate intact nuclei and high-molecular-weight (HMW) DNA. A total of 55,152 clones with an average insert size of 100 kb were picked. The frequency of BAC clones carrying inserts derived from chloroplast and mitochondrial DNA was estimated to be 1.5%. The coverage of the library is equivalent to 9.0-times the haploid genome. The BAC library was screened with 13 RFLP probes belonging to the 8 linkage groups of the consensus molecular map of banana. A total of 135 clones were identified giving an average of 10.38 clones for each locus. This BAC library will be a valuable starting tool for many of the goals of the recently emerged International Musa Genomic Consortium. One of our initial objectives will be to develop a banana physical map by BAC-FISH (fluorescent in situ hybridization) viewing the characterization of translocation break points.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-002-1155-2</identifier><identifier>PMID: 12671759</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Artificial chromosomes ; Bananas ; Biological and medical sciences ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Chromosomes, Artificial, Bacterial ; Cloning ; Consortia ; Crop diseases ; Crops ; Cytogenetics ; DNA, Chloroplast ; DNA, Mitochondrial ; Ethylenediaminetetraacetic acid ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Genetic aspects ; Genomes ; Genomics ; Hybridization ; Leaves ; Libraries ; Mitochondrial DNA ; Molecular and cellular biology ; Musa - genetics ; Polyphenols ; Polysaccharides ; Povidone</subject><ispartof>Theoretical and applied genetics, 2003-04, Vol.106 (6), p.1102-1106</ispartof><rights>2003 INIST-CNRS</rights><rights>COPYRIGHT 2003 Springer</rights><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-e96ff81ca457fcd83cde593ad75c500ee3bf3a8eacdfa0055e7ce08e033609983</citedby><cites>FETCH-LOGICAL-c419t-e96ff81ca457fcd83cde593ad75c500ee3bf3a8eacdfa0055e7ce08e033609983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14680889$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12671759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VILARINHOS, A. D</creatorcontrib><creatorcontrib>PIFFANELLI, P</creatorcontrib><creatorcontrib>LAGODA, P</creatorcontrib><creatorcontrib>THIBIVILLIERS, S</creatorcontrib><creatorcontrib>SABAU, X</creatorcontrib><creatorcontrib>CARREEL, F</creatorcontrib><creatorcontrib>D'HONT, A</creatorcontrib><title>Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>A bacterial artificial chromosome (BAC) library for banana was constructed from leaves of the wild diploid 'Calcutta 4' clone (Musa acuminata subsp. Burmannicoides 2n = 2 x = 22). 'Calcutta 4' is widely used in breeding programs for its resistance to the current major disease of banana and is being used to build a genetic reference map of banana. As banana leaves are particularly rich in polyphenols and polysaccharides a protocol was adapted to isolate intact nuclei and high-molecular-weight (HMW) DNA. A total of 55,152 clones with an average insert size of 100 kb were picked. The frequency of BAC clones carrying inserts derived from chloroplast and mitochondrial DNA was estimated to be 1.5%. The coverage of the library is equivalent to 9.0-times the haploid genome. The BAC library was screened with 13 RFLP probes belonging to the 8 linkage groups of the consensus molecular map of banana. A total of 135 clones were identified giving an average of 10.38 clones for each locus. This BAC library will be a valuable starting tool for many of the goals of the recently emerged International Musa Genomic Consortium. One of our initial objectives will be to develop a banana physical map by BAC-FISH (fluorescent in situ hybridization) viewing the characterization of translocation break points.</description><subject>Artificial chromosomes</subject><subject>Bananas</subject><subject>Biological and medical sciences</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Chromosomes, Artificial, Bacterial</subject><subject>Cloning</subject><subject>Consortia</subject><subject>Crop diseases</subject><subject>Crops</subject><subject>Cytogenetics</subject><subject>DNA, Chloroplast</subject><subject>DNA, Mitochondrial</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Hybridization</subject><subject>Leaves</subject><subject>Libraries</subject><subject>Mitochondrial DNA</subject><subject>Molecular and cellular biology</subject><subject>Musa - genetics</subject><subject>Polyphenols</subject><subject>Polysaccharides</subject><subject>Povidone</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0V1r3iAUB3AZG-uzbh9gNyMMNtaLbEeNUS_Lw14KLYVuu5YTo60l0U4T2PbpmzQPFHYzvFAOP_Xon5DXFD5SAPmpAFDGagBWUypEzZ6QHW04qxlr2FOyA2igFlKwI_KilFtYoAD-nBxR1koqhd6RtE-xTHm2U0ixwthX9gYz2snl8BcfislXWHVbCYcK8xR8sOvS3uQ0ppJGVw2hy5j_rLjDuIzqw8VcsEI7jyHihNU-DQOevCTPPA7FvTrMx-Tnl88_9t_q88uvZ_vT89o2VE-10633ilpshPS2V9z2TmiOvRRWADjHO89RObS9RwAhnLQOlAPOW9Ba8WPyfjv3LqdfsyuTGUOxbmkhujQXIzlttW7YfyFVshFa8gW-_QfepjnH5RFGMi4oUxIWdLKhaxycCdGmOLnf0zXOpZiz71fmtBWNZIw-3Ew3a3MqJTtv7nIYl080FMyar9nyNUtsZs3XrHveHJqYu9H1jzsOgS7g3QFgsTj4jNGG8uiaVoFSmt8D9R2tYw</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>VILARINHOS, A. 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Psychology</topic><topic>Gene Library</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Hybridization</topic><topic>Leaves</topic><topic>Libraries</topic><topic>Mitochondrial DNA</topic><topic>Molecular and cellular biology</topic><topic>Musa - genetics</topic><topic>Polyphenols</topic><topic>Polysaccharides</topic><topic>Povidone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VILARINHOS, A. 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D</au><au>PIFFANELLI, P</au><au>LAGODA, P</au><au>THIBIVILLIERS, S</au><au>SABAU, X</au><au>CARREEL, F</au><au>D'HONT, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>106</volume><issue>6</issue><spage>1102</spage><epage>1106</epage><pages>1102-1106</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>A bacterial artificial chromosome (BAC) library for banana was constructed from leaves of the wild diploid 'Calcutta 4' clone (Musa acuminata subsp. Burmannicoides 2n = 2 x = 22). 'Calcutta 4' is widely used in breeding programs for its resistance to the current major disease of banana and is being used to build a genetic reference map of banana. As banana leaves are particularly rich in polyphenols and polysaccharides a protocol was adapted to isolate intact nuclei and high-molecular-weight (HMW) DNA. A total of 55,152 clones with an average insert size of 100 kb were picked. The frequency of BAC clones carrying inserts derived from chloroplast and mitochondrial DNA was estimated to be 1.5%. The coverage of the library is equivalent to 9.0-times the haploid genome. The BAC library was screened with 13 RFLP probes belonging to the 8 linkage groups of the consensus molecular map of banana. A total of 135 clones were identified giving an average of 10.38 clones for each locus. This BAC library will be a valuable starting tool for many of the goals of the recently emerged International Musa Genomic Consortium. One of our initial objectives will be to develop a banana physical map by BAC-FISH (fluorescent in situ hybridization) viewing the characterization of translocation break points.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>12671759</pmid><doi>10.1007/s00122-002-1155-2</doi><tpages>5</tpages></addata></record> |
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| subjects | Artificial chromosomes Bananas Biological and medical sciences Cell differentiation, maturation, development, hematopoiesis Cell physiology Chromosomes, Artificial, Bacterial Cloning Consortia Crop diseases Crops Cytogenetics DNA, Chloroplast DNA, Mitochondrial Ethylenediaminetetraacetic acid Fundamental and applied biological sciences. Psychology Gene Library Genetic aspects Genomes Genomics Hybridization Leaves Libraries Mitochondrial DNA Molecular and cellular biology Musa - genetics Polyphenols Polysaccharides Povidone |
| title | Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla) |
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