3'-Azido-3'-deoxythymidine-induced reduction in the ability of uninfected CD4-expressing cells to participate in syncytium formation
A cocultivation assay system consisting of uninfected human T cells and cells chronically infected with human immunodeficiency virus type 1 has been used to investigate syncytium formation in short-term assays. Continuous treatment or short-term pretreatment of uninfected CD4-expressing human T-cell...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-09, Vol.89 (17), p.8361-8365 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | R W Buckheit, Jr J Germany-Decker K Qualls-Goodwin B J Bowdon W M Shannon |
| description | A cocultivation assay system consisting of uninfected human T cells and cells chronically infected with human immunodeficiency virus type 1 has been used to investigate syncytium formation in short-term assays. Continuous treatment or short-term pretreatment of uninfected CD4-expressing human T-cell lines with 3'-azido-3'-deoxythymidine (AZT) reduces the ability of these cells to participate in syncytium formation when mixed with chronically infected cells. The effect of AZT on syncytium formation is observed both as a reduction in the number of syncytia and as a reduction in the size of the syncytia that are detected. This syncytium-reducing effect of AZT is dose and time dependent and does not result from a modulation of CD4 antigen expression on the cell surface of uninfected, treated cells. Maximum syncytium reduction is observed with the continuous presence of AZT; however, pretreatment for times as short as 15 min results in a significant reduction in syncytium formation. Since reverse transcription is not required for efficient syncytium formation, the syncytium-reducing effect of AZT on uninfected human cells may represent an antiviral property of AZT with important therapeutic potential. |
| doi_str_mv | 10.1073/pnas.89.17.8361 |
| format | Article |
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Continuous treatment or short-term pretreatment of uninfected CD4-expressing human T-cell lines with 3'-azido-3'-deoxythymidine (AZT) reduces the ability of these cells to participate in syncytium formation when mixed with chronically infected cells. The effect of AZT on syncytium formation is observed both as a reduction in the number of syncytia and as a reduction in the size of the syncytia that are detected. This syncytium-reducing effect of AZT is dose and time dependent and does not result from a modulation of CD4 antigen expression on the cell surface of uninfected, treated cells. Maximum syncytium reduction is observed with the continuous presence of AZT; however, pretreatment for times as short as 15 min results in a significant reduction in syncytium formation. Since reverse transcription is not required for efficient syncytium formation, the syncytium-reducing effect of AZT on uninfected human cells may represent an antiviral property of AZT with important therapeutic potential.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.17.8361</identifier><identifier>PMID: 1355606</identifier><language>eng</language><publisher>United States: National Acad Sciences</publisher><subject>AIDS/HIV ; CD4-Positive T-Lymphocytes - cytology ; Cell Fusion - drug effects ; Cells, Cultured ; Drugs ; HIV ; HIV Infections - pathology ; Human immunodeficiency virus ; Humans ; In Vitro Techniques ; Medical research ; Time Factors ; Zidovudine - pharmacology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-09, Vol.89 (17), p.8361-8365</ispartof><rights>Copyright National Academy of Sciences Sep 1, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-47407510d5150848f2f9e2f98816641e8325785dc47edad4f9fbad7ef8155b4e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/17.cover.gif</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC49918/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC49918/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1355606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>R W Buckheit, Jr</creatorcontrib><creatorcontrib>J Germany-Decker</creatorcontrib><creatorcontrib>K Qualls-Goodwin</creatorcontrib><creatorcontrib>B J Bowdon</creatorcontrib><creatorcontrib>W M Shannon</creatorcontrib><title>3'-Azido-3'-deoxythymidine-induced reduction in the ability of uninfected CD4-expressing cells to participate in syncytium formation</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A cocultivation assay system consisting of uninfected human T cells and cells chronically infected with human immunodeficiency virus type 1 has been used to investigate syncytium formation in short-term assays. 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Since reverse transcription is not required for efficient syncytium formation, the syncytium-reducing effect of AZT on uninfected human cells may represent an antiviral property of AZT with important therapeutic potential.</description><subject>AIDS/HIV</subject><subject>CD4-Positive T-Lymphocytes - cytology</subject><subject>Cell Fusion - drug effects</subject><subject>Cells, Cultured</subject><subject>Drugs</subject><subject>HIV</subject><subject>HIV Infections - pathology</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Medical research</subject><subject>Time Factors</subject><subject>Zidovudine - pharmacology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2P1CAYxhujWcfVsycN8eCeOgstFJrsZTPrV7KJFz0TprzssGmhAjVTz_7hS53xYz3ogbwkz-954IW3KJ4TvCaY1-ejU3Et2jXha1E35EGxIrglZUNb_LBYYVzxUtCKPi6exHiLMW6ZwCfFCakZa3CzKr7XZ-XlN6t9mTca_H5Ou3mw2joordNTBxoFyDVZ75B1KO0Aqa3tbZqRN2hy1hnoUsY2V7SE_RggRutuUAd9H1HyaFQh2c6OKsESEGfXzclOAzI-DGrJfVo8MqqP8OxYT4vPb9982rwvrz---7C5vC472rappJxizgjWjDAsqDCVaSEvIUjTUAKirhgXTHeUg1aamtZsleZgBGFsS6E-LS4OueO0HUB34FJQvRyDHVSYpVdW3lec3ckb_1Xm44nI9tdHe_BfJohJDjYubSoHfoqS16RpCeP_BUlTEy6aJoOv_gJv_RRcfgNZYVIJzn5A5weoCz7GAObXhQmWyxDIZQikaCXhchmC7HjxZ5-_-cOvZ_3lUV-MP9V7AWf_BKSZ-j7BPtV3C-vIAw</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>R W Buckheit, Jr</creator><creator>J Germany-Decker</creator><creator>K Qualls-Goodwin</creator><creator>B J Bowdon</creator><creator>W M Shannon</creator><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920901</creationdate><title>3'-Azido-3'-deoxythymidine-induced reduction in the ability of uninfected CD4-expressing cells to participate in syncytium formation</title><author>R W Buckheit, Jr ; 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Continuous treatment or short-term pretreatment of uninfected CD4-expressing human T-cell lines with 3'-azido-3'-deoxythymidine (AZT) reduces the ability of these cells to participate in syncytium formation when mixed with chronically infected cells. The effect of AZT on syncytium formation is observed both as a reduction in the number of syncytia and as a reduction in the size of the syncytia that are detected. This syncytium-reducing effect of AZT is dose and time dependent and does not result from a modulation of CD4 antigen expression on the cell surface of uninfected, treated cells. Maximum syncytium reduction is observed with the continuous presence of AZT; however, pretreatment for times as short as 15 min results in a significant reduction in syncytium formation. 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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | AIDS/HIV CD4-Positive T-Lymphocytes - cytology Cell Fusion - drug effects Cells, Cultured Drugs HIV HIV Infections - pathology Human immunodeficiency virus Humans In Vitro Techniques Medical research Time Factors Zidovudine - pharmacology |
| title | 3'-Azido-3'-deoxythymidine-induced reduction in the ability of uninfected CD4-expressing cells to participate in syncytium formation |
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