Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition
JC virus (JCV) is the causative agent of the demyelinating disease progressive multifocal leucoencephalopathy (PML), which can be diagnosed by detection in the cerebrospinal fluid (CSF) of both JCV DNA and intrathecally-produced anti-JCV antibody. However, the restricted in-vitro species and cell tr...
Gespeichert in:
Veröffentlicht in: | Journal of virological methods 2003-04, Vol.109 (1), p.47-54 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 54 |
---|---|
container_issue | 1 |
container_start_page | 47 |
container_title | Journal of virological methods |
container_volume | 109 |
creator | Knowles, W.A Sasnauskas, K |
description | JC virus (JCV) is the causative agent of the demyelinating disease progressive multifocal leucoencephalopathy (PML), which can be diagnosed by detection in the cerebrospinal fluid (CSF) of both JCV DNA and intrathecally-produced anti-JCV antibody. However, the restricted in-vitro species and cell tropism shown by JCV has made antigen production difficult and limited serological investigations both in PML diagnosis and for JCV epidemiology. In this study antigen prepared as a crude cell lysate of JCV-infected primary human fetal glial (PHFG) cells was compared in a haemagglutination inhibition (HI) assay with antigen produced from the JCV carrier cell line, JCI, and yeast-expressed JCV VP1. Forty-two sera were tested with each antigen and there was a high level of correlation between the assays: 96.5% between the HI assays with PHFG and JCI antigens and 98.1% between the HI assays with PHFG and recombinant VP1 (rVP1) antigens. The JCI antigen gave HI titres 19% lower than the PHFG antigen (
P=0.022). Titres with the rVP1 antigen were 2% higher than with the PHFG antigen (
P=0.83). When serum/CSF pairs from 11 PML patients were tested, the antibody index calculated in each case confirmed the production of intrathecal anti-JCV antibody. Antibody testing for JCV is no longer reliant on PHFG cells and JCV serological tests should be available more widely. |
doi_str_mv | 10.1016/S0166-0934(03)00043-0 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73162524</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0166093403000430</els_id><sourcerecordid>18746061</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-7c4feb9ab6d393b5b703de2cb9ec1f2a7899aa20df925cc87a49c9f554447ade3</originalsourceid><addsrcrecordid>eNqFkd2O1SAUhYnROMejj6DhRjNzUaVAaXtlzIk_YybRRJ1bQuluD6aFM0DHzDP6UtKfOJdzAzubb60NLIRe5uRtTnLx7kdaREZqxs8JuyCEcJaRR2iXV2Wd2hV_jHb_kTP0LITfCSpKxp6is5wKUVFR7tDfgxtPypvgLHYd1jAMWE9DnDxkvXd_LP56wLfGTwGfn7wZlb_Dx2lUFncQ1YD7waR1lgWsbIvjEZLicjUajIWLpe1Bu7ExVtmYjq_x9fccq1kRTQ_Jy_lF2UIEHc16l_kwm-G5aFx7h5s0W8Go-n6YYjJbSGOPpjFz-Rw96dQQ4MW279GvTx9_Hr5kV98-Xx4-XGWaUxqzUvMOmlo1omU1a4qmJKwFqpsadN5RVVZ1rRQlbVfTQuuqVLzWdVcUnPNStcD26M3qe_LuZoIQ5WjC_GBlwU1BliwXtKD8QTBlxQUReQKLFdTeheChk9tfy5zIOW65xC3nLCVhcok7FXv0ahswNSO096ot3wS83gAVtBo6r6w24Z7jggtBaeLerxykf7s14GXQBqyG1qToomydeeAq_wDA5snd</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18746061</pqid></control><display><type>article</type><title>Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Knowles, W.A ; Sasnauskas, K</creator><creatorcontrib>Knowles, W.A ; Sasnauskas, K</creatorcontrib><description>JC virus (JCV) is the causative agent of the demyelinating disease progressive multifocal leucoencephalopathy (PML), which can be diagnosed by detection in the cerebrospinal fluid (CSF) of both JCV DNA and intrathecally-produced anti-JCV antibody. However, the restricted in-vitro species and cell tropism shown by JCV has made antigen production difficult and limited serological investigations both in PML diagnosis and for JCV epidemiology. In this study antigen prepared as a crude cell lysate of JCV-infected primary human fetal glial (PHFG) cells was compared in a haemagglutination inhibition (HI) assay with antigen produced from the JCV carrier cell line, JCI, and yeast-expressed JCV VP1. Forty-two sera were tested with each antigen and there was a high level of correlation between the assays: 96.5% between the HI assays with PHFG and JCI antigens and 98.1% between the HI assays with PHFG and recombinant VP1 (rVP1) antigens. The JCI antigen gave HI titres 19% lower than the PHFG antigen (
P=0.022). Titres with the rVP1 antigen were 2% higher than with the PHFG antigen (
P=0.83). When serum/CSF pairs from 11 PML patients were tested, the antibody index calculated in each case confirmed the production of intrathecal anti-JCV antibody. Antibody testing for JCV is no longer reliant on PHFG cells and JCV serological tests should be available more widely.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/S0166-0934(03)00043-0</identifier><identifier>PMID: 12668267</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anti-JCV antibody ; Antibodies, Viral - blood ; Antibodies, Viral - cerebrospinal fluid ; Antigens, Viral - immunology ; Biological and medical sciences ; Cell Line ; Cells, Cultured ; Child ; Child, Preschool ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - immunology ; Fundamental and applied biological sciences. Psychology ; Haemagglutination inhibition ; Hemagglutination Inhibition Tests ; Human viral diseases ; Humans ; Infectious diseases ; JC Virus - genetics ; JC Virus - immunology ; JC Virus - physiology ; JCI cell line ; JCV antigen ; Leukoencephalopathy, Progressive Multifocal - diagnosis ; Leukoencephalopathy, Progressive Multifocal - virology ; Medical sciences ; Microbiology ; Middle Aged ; Neuroglia - virology ; Plant Proteins ; Primary human fetal glial cells ; Recombinant Proteins - immunology ; Techniques used in virology ; Trans-Activators ; Transcription Factors - genetics ; Transcription Factors - immunology ; Viral diseases ; Viral diseases of the nervous system ; Virology ; Virus Cultivation - methods ; Yeast-expressed recombinant JCV VP1</subject><ispartof>Journal of virological methods, 2003-04, Vol.109 (1), p.47-54</ispartof><rights>2003 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-7c4feb9ab6d393b5b703de2cb9ec1f2a7899aa20df925cc87a49c9f554447ade3</citedby><cites>FETCH-LOGICAL-c422t-7c4feb9ab6d393b5b703de2cb9ec1f2a7899aa20df925cc87a49c9f554447ade3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0166-0934(03)00043-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14646622$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12668267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Knowles, W.A</creatorcontrib><creatorcontrib>Sasnauskas, K</creatorcontrib><title>Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>JC virus (JCV) is the causative agent of the demyelinating disease progressive multifocal leucoencephalopathy (PML), which can be diagnosed by detection in the cerebrospinal fluid (CSF) of both JCV DNA and intrathecally-produced anti-JCV antibody. However, the restricted in-vitro species and cell tropism shown by JCV has made antigen production difficult and limited serological investigations both in PML diagnosis and for JCV epidemiology. In this study antigen prepared as a crude cell lysate of JCV-infected primary human fetal glial (PHFG) cells was compared in a haemagglutination inhibition (HI) assay with antigen produced from the JCV carrier cell line, JCI, and yeast-expressed JCV VP1. Forty-two sera were tested with each antigen and there was a high level of correlation between the assays: 96.5% between the HI assays with PHFG and JCI antigens and 98.1% between the HI assays with PHFG and recombinant VP1 (rVP1) antigens. The JCI antigen gave HI titres 19% lower than the PHFG antigen (
P=0.022). Titres with the rVP1 antigen were 2% higher than with the PHFG antigen (
P=0.83). When serum/CSF pairs from 11 PML patients were tested, the antibody index calculated in each case confirmed the production of intrathecal anti-JCV antibody. Antibody testing for JCV is no longer reliant on PHFG cells and JCV serological tests should be available more widely.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Anti-JCV antibody</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - cerebrospinal fluid</subject><subject>Antigens, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Haemagglutination inhibition</subject><subject>Hemagglutination Inhibition Tests</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>JC Virus - genetics</subject><subject>JC Virus - immunology</subject><subject>JC Virus - physiology</subject><subject>JCI cell line</subject><subject>JCV antigen</subject><subject>Leukoencephalopathy, Progressive Multifocal - diagnosis</subject><subject>Leukoencephalopathy, Progressive Multifocal - virology</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Neuroglia - virology</subject><subject>Plant Proteins</subject><subject>Primary human fetal glial cells</subject><subject>Recombinant Proteins - immunology</subject><subject>Techniques used in virology</subject><subject>Trans-Activators</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - immunology</subject><subject>Viral diseases</subject><subject>Viral diseases of the nervous system</subject><subject>Virology</subject><subject>Virus Cultivation - methods</subject><subject>Yeast-expressed recombinant JCV VP1</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2O1SAUhYnROMejj6DhRjNzUaVAaXtlzIk_YybRRJ1bQuluD6aFM0DHzDP6UtKfOJdzAzubb60NLIRe5uRtTnLx7kdaREZqxs8JuyCEcJaRR2iXV2Wd2hV_jHb_kTP0LITfCSpKxp6is5wKUVFR7tDfgxtPypvgLHYd1jAMWE9DnDxkvXd_LP56wLfGTwGfn7wZlb_Dx2lUFncQ1YD7waR1lgWsbIvjEZLicjUajIWLpe1Bu7ExVtmYjq_x9fccq1kRTQ_Jy_lF2UIEHc16l_kwm-G5aFx7h5s0W8Go-n6YYjJbSGOPpjFz-Rw96dQQ4MW279GvTx9_Hr5kV98-Xx4-XGWaUxqzUvMOmlo1omU1a4qmJKwFqpsadN5RVVZ1rRQlbVfTQuuqVLzWdVcUnPNStcD26M3qe_LuZoIQ5WjC_GBlwU1BliwXtKD8QTBlxQUReQKLFdTeheChk9tfy5zIOW65xC3nLCVhcok7FXv0ahswNSO096ot3wS83gAVtBo6r6w24Z7jggtBaeLerxykf7s14GXQBqyG1qToomydeeAq_wDA5snd</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Knowles, W.A</creator><creator>Sasnauskas, K</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition</title><author>Knowles, W.A ; Sasnauskas, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-7c4feb9ab6d393b5b703de2cb9ec1f2a7899aa20df925cc87a49c9f554447ade3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Anti-JCV antibody</topic><topic>Antibodies, Viral - blood</topic><topic>Antibodies, Viral - cerebrospinal fluid</topic><topic>Antigens, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Haemagglutination inhibition</topic><topic>Hemagglutination Inhibition Tests</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>JC Virus - genetics</topic><topic>JC Virus - immunology</topic><topic>JC Virus - physiology</topic><topic>JCI cell line</topic><topic>JCV antigen</topic><topic>Leukoencephalopathy, Progressive Multifocal - diagnosis</topic><topic>Leukoencephalopathy, Progressive Multifocal - virology</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Neuroglia - virology</topic><topic>Plant Proteins</topic><topic>Primary human fetal glial cells</topic><topic>Recombinant Proteins - immunology</topic><topic>Techniques used in virology</topic><topic>Trans-Activators</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - immunology</topic><topic>Viral diseases</topic><topic>Viral diseases of the nervous system</topic><topic>Virology</topic><topic>Virus Cultivation - methods</topic><topic>Yeast-expressed recombinant JCV VP1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Knowles, W.A</creatorcontrib><creatorcontrib>Sasnauskas, K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Knowles, W.A</au><au>Sasnauskas, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>109</volume><issue>1</issue><spage>47</spage><epage>54</epage><pages>47-54</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>JC virus (JCV) is the causative agent of the demyelinating disease progressive multifocal leucoencephalopathy (PML), which can be diagnosed by detection in the cerebrospinal fluid (CSF) of both JCV DNA and intrathecally-produced anti-JCV antibody. However, the restricted in-vitro species and cell tropism shown by JCV has made antigen production difficult and limited serological investigations both in PML diagnosis and for JCV epidemiology. In this study antigen prepared as a crude cell lysate of JCV-infected primary human fetal glial (PHFG) cells was compared in a haemagglutination inhibition (HI) assay with antigen produced from the JCV carrier cell line, JCI, and yeast-expressed JCV VP1. Forty-two sera were tested with each antigen and there was a high level of correlation between the assays: 96.5% between the HI assays with PHFG and JCI antigens and 98.1% between the HI assays with PHFG and recombinant VP1 (rVP1) antigens. The JCI antigen gave HI titres 19% lower than the PHFG antigen (
P=0.022). Titres with the rVP1 antigen were 2% higher than with the PHFG antigen (
P=0.83). When serum/CSF pairs from 11 PML patients were tested, the antibody index calculated in each case confirmed the production of intrathecal anti-JCV antibody. Antibody testing for JCV is no longer reliant on PHFG cells and JCV serological tests should be available more widely.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>12668267</pmid><doi>10.1016/S0166-0934(03)00043-0</doi><tpages>8</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0166-0934 |
ispartof | Journal of virological methods, 2003-04, Vol.109 (1), p.47-54 |
issn | 0166-0934 1879-0984 |
language | eng |
recordid | cdi_proquest_miscellaneous_73162524 |
source | MEDLINE; Elsevier ScienceDirect Journals Complete |
subjects | Adolescent Adult Aged Aged, 80 and over Anti-JCV antibody Antibodies, Viral - blood Antibodies, Viral - cerebrospinal fluid Antigens, Viral - immunology Biological and medical sciences Cell Line Cells, Cultured Child Child, Preschool DNA-Binding Proteins - genetics DNA-Binding Proteins - immunology Fundamental and applied biological sciences. Psychology Haemagglutination inhibition Hemagglutination Inhibition Tests Human viral diseases Humans Infectious diseases JC Virus - genetics JC Virus - immunology JC Virus - physiology JCI cell line JCV antigen Leukoencephalopathy, Progressive Multifocal - diagnosis Leukoencephalopathy, Progressive Multifocal - virology Medical sciences Microbiology Middle Aged Neuroglia - virology Plant Proteins Primary human fetal glial cells Recombinant Proteins - immunology Techniques used in virology Trans-Activators Transcription Factors - genetics Transcription Factors - immunology Viral diseases Viral diseases of the nervous system Virology Virus Cultivation - methods Yeast-expressed recombinant JCV VP1 |
title | Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T10%3A07%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20cell%20culture-grown%20JC%20virus%20(primary%20human%20fetal%20glial%20cells%20and%20the%20JCI%20cell%20line)%20and%20recombinant%20JCV%20VP1%20as%20antigen%20for%20the%20detection%20of%20anti-JCV%20antibody%20by%20haemagglutination%20inhibition&rft.jtitle=Journal%20of%20virological%20methods&rft.au=Knowles,%20W.A&rft.date=2003-04-01&rft.volume=109&rft.issue=1&rft.spage=47&rft.epage=54&rft.pages=47-54&rft.issn=0166-0934&rft.eissn=1879-0984&rft.coden=JVMEDH&rft_id=info:doi/10.1016/S0166-0934(03)00043-0&rft_dat=%3Cproquest_cross%3E18746061%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18746061&rft_id=info:pmid/12668267&rft_els_id=S0166093403000430&rfr_iscdi=true |