Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition

JC virus (JCV) is the causative agent of the demyelinating disease progressive multifocal leucoencephalopathy (PML), which can be diagnosed by detection in the cerebrospinal fluid (CSF) of both JCV DNA and intrathecally-produced anti-JCV antibody. However, the restricted in-vitro species and cell tr...

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Veröffentlicht in:Journal of virological methods 2003-04, Vol.109 (1), p.47-54
Hauptverfasser: Knowles, W.A, Sasnauskas, K
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description JC virus (JCV) is the causative agent of the demyelinating disease progressive multifocal leucoencephalopathy (PML), which can be diagnosed by detection in the cerebrospinal fluid (CSF) of both JCV DNA and intrathecally-produced anti-JCV antibody. However, the restricted in-vitro species and cell tropism shown by JCV has made antigen production difficult and limited serological investigations both in PML diagnosis and for JCV epidemiology. In this study antigen prepared as a crude cell lysate of JCV-infected primary human fetal glial (PHFG) cells was compared in a haemagglutination inhibition (HI) assay with antigen produced from the JCV carrier cell line, JCI, and yeast-expressed JCV VP1. Forty-two sera were tested with each antigen and there was a high level of correlation between the assays: 96.5% between the HI assays with PHFG and JCI antigens and 98.1% between the HI assays with PHFG and recombinant VP1 (rVP1) antigens. The JCI antigen gave HI titres 19% lower than the PHFG antigen ( P=0.022). Titres with the rVP1 antigen were 2% higher than with the PHFG antigen ( P=0.83). When serum/CSF pairs from 11 PML patients were tested, the antibody index calculated in each case confirmed the production of intrathecal anti-JCV antibody. Antibody testing for JCV is no longer reliant on PHFG cells and JCV serological tests should be available more widely.
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Psychology</subject><subject>Haemagglutination inhibition</subject><subject>Hemagglutination Inhibition Tests</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>JC Virus - genetics</subject><subject>JC Virus - immunology</subject><subject>JC Virus - physiology</subject><subject>JCI cell line</subject><subject>JCV antigen</subject><subject>Leukoencephalopathy, Progressive Multifocal - diagnosis</subject><subject>Leukoencephalopathy, Progressive Multifocal - virology</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Neuroglia - virology</subject><subject>Plant Proteins</subject><subject>Primary human fetal glial cells</subject><subject>Recombinant Proteins - immunology</subject><subject>Techniques used in virology</subject><subject>Trans-Activators</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - immunology</subject><subject>Viral diseases</subject><subject>Viral diseases of the nervous system</subject><subject>Virology</subject><subject>Virus Cultivation - methods</subject><subject>Yeast-expressed recombinant JCV VP1</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2O1SAUhYnROMejj6DhRjNzUaVAaXtlzIk_YybRRJ1bQuluD6aFM0DHzDP6UtKfOJdzAzubb60NLIRe5uRtTnLx7kdaREZqxs8JuyCEcJaRR2iXV2Wd2hV_jHb_kTP0LITfCSpKxp6is5wKUVFR7tDfgxtPypvgLHYd1jAMWE9DnDxkvXd_LP56wLfGTwGfn7wZlb_Dx2lUFncQ1YD7waR1lgWsbIvjEZLicjUajIWLpe1Bu7ExVtmYjq_x9fccq1kRTQ_Jy_lF2UIEHc16l_kwm-G5aFx7h5s0W8Go-n6YYjJbSGOPpjFz-Rw96dQQ4MW279GvTx9_Hr5kV98-Xx4-XGWaUxqzUvMOmlo1omU1a4qmJKwFqpsadN5RVVZ1rRQlbVfTQuuqVLzWdVcUnPNStcD26M3qe_LuZoIQ5WjC_GBlwU1BliwXtKD8QTBlxQUReQKLFdTeheChk9tfy5zIOW65xC3nLCVhcok7FXv0ahswNSO096ot3wS83gAVtBo6r6w24Z7jggtBaeLerxykf7s14GXQBqyG1qToomydeeAq_wDA5snd</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Knowles, W.A</creator><creator>Sasnauskas, K</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition</title><author>Knowles, W.A ; Sasnauskas, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-7c4feb9ab6d393b5b703de2cb9ec1f2a7899aa20df925cc87a49c9f554447ade3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Anti-JCV antibody</topic><topic>Antibodies, Viral - blood</topic><topic>Antibodies, Viral - cerebrospinal fluid</topic><topic>Antigens, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - immunology</topic><topic>Fundamental and applied biological sciences. 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When serum/CSF pairs from 11 PML patients were tested, the antibody index calculated in each case confirmed the production of intrathecal anti-JCV antibody. Antibody testing for JCV is no longer reliant on PHFG cells and JCV serological tests should be available more widely.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>12668267</pmid><doi>10.1016/S0166-0934(03)00043-0</doi><tpages>8</tpages></addata></record>
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subjects Adolescent
Adult
Aged
Aged, 80 and over
Anti-JCV antibody
Antibodies, Viral - blood
Antibodies, Viral - cerebrospinal fluid
Antigens, Viral - immunology
Biological and medical sciences
Cell Line
Cells, Cultured
Child
Child, Preschool
DNA-Binding Proteins - genetics
DNA-Binding Proteins - immunology
Fundamental and applied biological sciences. Psychology
Haemagglutination inhibition
Hemagglutination Inhibition Tests
Human viral diseases
Humans
Infectious diseases
JC Virus - genetics
JC Virus - immunology
JC Virus - physiology
JCI cell line
JCV antigen
Leukoencephalopathy, Progressive Multifocal - diagnosis
Leukoencephalopathy, Progressive Multifocal - virology
Medical sciences
Microbiology
Middle Aged
Neuroglia - virology
Plant Proteins
Primary human fetal glial cells
Recombinant Proteins - immunology
Techniques used in virology
Trans-Activators
Transcription Factors - genetics
Transcription Factors - immunology
Viral diseases
Viral diseases of the nervous system
Virology
Virus Cultivation - methods
Yeast-expressed recombinant JCV VP1
title Comparison of cell culture-grown JC virus (primary human fetal glial cells and the JCI cell line) and recombinant JCV VP1 as antigen for the detection of anti-JCV antibody by haemagglutination inhibition
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