Genetic, morphological, and virulence characterization of the entomopathogenic fungus Verticillium lecanii
In order to clarify relationships among genetic diversity, virulence, and other characteristics of conidia, 46 isolates of Verticillium lecanii from various hosts and geographical locations were examined. The internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA (rDN...
Gespeichert in:
| Veröffentlicht in: | Journal of invertebrate pathology 2003-03, Vol.82 (3), p.176-187 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 187 |
|---|---|
| container_issue | 3 |
| container_start_page | 176 |
| container_title | Journal of invertebrate pathology |
| container_volume | 82 |
| creator | Sugimoto, Midori Koike, Masanori Hiyama, Naomi Nagao, Hideyuki |
| description | In order to clarify relationships among genetic diversity, virulence, and other characteristics of conidia, 46 isolates of
Verticillium lecanii from various hosts and geographical locations were examined. The internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA (rDNA), mitochondrial small subunit rDNA (mt-SrDNA) and β-tubulin were analyzed by PCR-RFLP. PCR-single stranded conformational polymorphism (SSCP) was performed on regions of the mitochondrial large subunit rDNA, mt-SrDNA, β-tubulin and histone 4. There were no relationships among the results of RFLP, SSCP, isolation source, and location. However, amplified product size of IGS did have relationships with conidia size and sporulation. Six isolates with 4.0-kb IGS products had large conidia dimensions, and yielded low numbers of conidia compared with other isolates. Three out of the six isolates were high virulence (over 90%) against green peach aphids. Furthermore, double-stranded RNA (dsRNA) was detected in 22 out of 35
V. lecanii isolates and related with the amplicon sizes of IGS, though not with virulence or isolation location. Isolates containing dsRNA were divided into six distinct types based on banding pattern. These data demonstrate the level of genetic diversity of
V. lecanii, and suggest relations among the genetic properties and conidial morphology. |
| doi_str_mv | 10.1016/S0022-2011(03)00014-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73161994</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022201103000144</els_id><sourcerecordid>18747034</sourcerecordid><originalsourceid>FETCH-LOGICAL-c488t-339ade458c4615dddf58232f47d2fc4faf6335966038d2ab2c528e54fb5192313</originalsourceid><addsrcrecordid>eNqF0U9rFDEYBvAgit1WP4KSi6LQ0fyfmVORUqtQ6KHqNWSTN7spM8maZAr10zvTXdpjT7n83vcNz4PQO0q-UELV1xtCGGsYofQT4Z8JIVQ04gVaUdKrhnREvkSrR3KEjku5nQ2Vqn-NjihTrZJSrNDtJUSowZ7iMeXdNg1pE6wZTrGJDt-FPA0QLWC7NdnYCjn8MzWkiJPHdQsYYk1j2pm6TRuIwWI_xc1U8B_I89IwDGEa8QDWxBDeoFfeDAXeHt4T9Pv7xa_zH83V9eXP829XjRVdVxvOe-NAyM4KRaVzzsuOceZF65i3whuvOJe9UoR3jpk1s5J1IIVfS9ozTvkJ-rjfu8vp7wSl6jEUC8NgIqSp6JZTRftePAtp14qW8AXKPbQ5lZLB610Oo8n3mhK9tKEf2tBL1Jpw_dCGXubeHw5M6xHc09Qh_hl8OABT5tR9NtGG8uTm85KyfnZnewdzbncBsi42LMW4kMFW7VJ45iv_AXHOp1g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18747034</pqid></control><display><type>article</type><title>Genetic, morphological, and virulence characterization of the entomopathogenic fungus Verticillium lecanii</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Sugimoto, Midori ; Koike, Masanori ; Hiyama, Naomi ; Nagao, Hideyuki</creator><creatorcontrib>Sugimoto, Midori ; Koike, Masanori ; Hiyama, Naomi ; Nagao, Hideyuki</creatorcontrib><description>In order to clarify relationships among genetic diversity, virulence, and other characteristics of conidia, 46 isolates of
Verticillium lecanii from various hosts and geographical locations were examined. The internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA (rDNA), mitochondrial small subunit rDNA (mt-SrDNA) and β-tubulin were analyzed by PCR-RFLP. PCR-single stranded conformational polymorphism (SSCP) was performed on regions of the mitochondrial large subunit rDNA, mt-SrDNA, β-tubulin and histone 4. There were no relationships among the results of RFLP, SSCP, isolation source, and location. However, amplified product size of IGS did have relationships with conidia size and sporulation. Six isolates with 4.0-kb IGS products had large conidia dimensions, and yielded low numbers of conidia compared with other isolates. Three out of the six isolates were high virulence (over 90%) against green peach aphids. Furthermore, double-stranded RNA (dsRNA) was detected in 22 out of 35
V. lecanii isolates and related with the amplicon sizes of IGS, though not with virulence or isolation location. Isolates containing dsRNA were divided into six distinct types based on banding pattern. These data demonstrate the level of genetic diversity of
V. lecanii, and suggest relations among the genetic properties and conidial morphology.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/S0022-2011(03)00014-4</identifier><identifier>PMID: 12676554</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Aphids - parasitology ; Biological and medical sciences ; Biological control ; Claviceps - genetics ; Claviceps - isolation & purification ; Claviceps - pathogenicity ; Control ; DNA, Fungal - analysis ; Double-stranded RNA ; Fundamental and applied biological sciences. Psychology ; Histone 4 ; Morphology ; Pathogenicity ; Pest Control, Biological ; Phytopathology. Animal pests. Plant and forest protection ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single-Stranded Conformational ; Protozoa. Invertebrates ; Ribosomal DNA ; Verticillium lecanii ; β-Tubulin</subject><ispartof>Journal of invertebrate pathology, 2003-03, Vol.82 (3), p.176-187</ispartof><rights>2003 Elsevier Science (USA)</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-339ade458c4615dddf58232f47d2fc4faf6335966038d2ab2c528e54fb5192313</citedby><cites>FETCH-LOGICAL-c488t-339ade458c4615dddf58232f47d2fc4faf6335966038d2ab2c528e54fb5192313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0022-2011(03)00014-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14705129$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12676554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugimoto, Midori</creatorcontrib><creatorcontrib>Koike, Masanori</creatorcontrib><creatorcontrib>Hiyama, Naomi</creatorcontrib><creatorcontrib>Nagao, Hideyuki</creatorcontrib><title>Genetic, morphological, and virulence characterization of the entomopathogenic fungus Verticillium lecanii</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>In order to clarify relationships among genetic diversity, virulence, and other characteristics of conidia, 46 isolates of
Verticillium lecanii from various hosts and geographical locations were examined. The internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA (rDNA), mitochondrial small subunit rDNA (mt-SrDNA) and β-tubulin were analyzed by PCR-RFLP. PCR-single stranded conformational polymorphism (SSCP) was performed on regions of the mitochondrial large subunit rDNA, mt-SrDNA, β-tubulin and histone 4. There were no relationships among the results of RFLP, SSCP, isolation source, and location. However, amplified product size of IGS did have relationships with conidia size and sporulation. Six isolates with 4.0-kb IGS products had large conidia dimensions, and yielded low numbers of conidia compared with other isolates. Three out of the six isolates were high virulence (over 90%) against green peach aphids. Furthermore, double-stranded RNA (dsRNA) was detected in 22 out of 35
V. lecanii isolates and related with the amplicon sizes of IGS, though not with virulence or isolation location. Isolates containing dsRNA were divided into six distinct types based on banding pattern. These data demonstrate the level of genetic diversity of
V. lecanii, and suggest relations among the genetic properties and conidial morphology.</description><subject>Animals</subject><subject>Aphids - parasitology</subject><subject>Biological and medical sciences</subject><subject>Biological control</subject><subject>Claviceps - genetics</subject><subject>Claviceps - isolation & purification</subject><subject>Claviceps - pathogenicity</subject><subject>Control</subject><subject>DNA, Fungal - analysis</subject><subject>Double-stranded RNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histone 4</subject><subject>Morphology</subject><subject>Pathogenicity</subject><subject>Pest Control, Biological</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Protozoa. Invertebrates</subject><subject>Ribosomal DNA</subject><subject>Verticillium lecanii</subject><subject>β-Tubulin</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U9rFDEYBvAgit1WP4KSi6LQ0fyfmVORUqtQ6KHqNWSTN7spM8maZAr10zvTXdpjT7n83vcNz4PQO0q-UELV1xtCGGsYofQT4Z8JIVQ04gVaUdKrhnREvkSrR3KEjku5nQ2Vqn-NjihTrZJSrNDtJUSowZ7iMeXdNg1pE6wZTrGJDt-FPA0QLWC7NdnYCjn8MzWkiJPHdQsYYk1j2pm6TRuIwWI_xc1U8B_I89IwDGEa8QDWxBDeoFfeDAXeHt4T9Pv7xa_zH83V9eXP829XjRVdVxvOe-NAyM4KRaVzzsuOceZF65i3whuvOJe9UoR3jpk1s5J1IIVfS9ozTvkJ-rjfu8vp7wSl6jEUC8NgIqSp6JZTRftePAtp14qW8AXKPbQ5lZLB610Oo8n3mhK9tKEf2tBL1Jpw_dCGXubeHw5M6xHc09Qh_hl8OABT5tR9NtGG8uTm85KyfnZnewdzbncBsi42LMW4kMFW7VJ45iv_AXHOp1g</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Sugimoto, Midori</creator><creator>Koike, Masanori</creator><creator>Hiyama, Naomi</creator><creator>Nagao, Hideyuki</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Genetic, morphological, and virulence characterization of the entomopathogenic fungus Verticillium lecanii</title><author>Sugimoto, Midori ; Koike, Masanori ; Hiyama, Naomi ; Nagao, Hideyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-339ade458c4615dddf58232f47d2fc4faf6335966038d2ab2c528e54fb5192313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Aphids - parasitology</topic><topic>Biological and medical sciences</topic><topic>Biological control</topic><topic>Claviceps - genetics</topic><topic>Claviceps - isolation & purification</topic><topic>Claviceps - pathogenicity</topic><topic>Control</topic><topic>DNA, Fungal - analysis</topic><topic>Double-stranded RNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histone 4</topic><topic>Morphology</topic><topic>Pathogenicity</topic><topic>Pest Control, Biological</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Protozoa. Invertebrates</topic><topic>Ribosomal DNA</topic><topic>Verticillium lecanii</topic><topic>β-Tubulin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugimoto, Midori</creatorcontrib><creatorcontrib>Koike, Masanori</creatorcontrib><creatorcontrib>Hiyama, Naomi</creatorcontrib><creatorcontrib>Nagao, Hideyuki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugimoto, Midori</au><au>Koike, Masanori</au><au>Hiyama, Naomi</au><au>Nagao, Hideyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic, morphological, and virulence characterization of the entomopathogenic fungus Verticillium lecanii</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>82</volume><issue>3</issue><spage>176</spage><epage>187</epage><pages>176-187</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>In order to clarify relationships among genetic diversity, virulence, and other characteristics of conidia, 46 isolates of
Verticillium lecanii from various hosts and geographical locations were examined. The internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA (rDNA), mitochondrial small subunit rDNA (mt-SrDNA) and β-tubulin were analyzed by PCR-RFLP. PCR-single stranded conformational polymorphism (SSCP) was performed on regions of the mitochondrial large subunit rDNA, mt-SrDNA, β-tubulin and histone 4. There were no relationships among the results of RFLP, SSCP, isolation source, and location. However, amplified product size of IGS did have relationships with conidia size and sporulation. Six isolates with 4.0-kb IGS products had large conidia dimensions, and yielded low numbers of conidia compared with other isolates. Three out of the six isolates were high virulence (over 90%) against green peach aphids. Furthermore, double-stranded RNA (dsRNA) was detected in 22 out of 35
V. lecanii isolates and related with the amplicon sizes of IGS, though not with virulence or isolation location. Isolates containing dsRNA were divided into six distinct types based on banding pattern. These data demonstrate the level of genetic diversity of
V. lecanii, and suggest relations among the genetic properties and conidial morphology.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>12676554</pmid><doi>10.1016/S0022-2011(03)00014-4</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2011 |
| ispartof | Journal of invertebrate pathology, 2003-03, Vol.82 (3), p.176-187 |
| issn | 0022-2011 1096-0805 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73161994 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Aphids - parasitology Biological and medical sciences Biological control Claviceps - genetics Claviceps - isolation & purification Claviceps - pathogenicity Control DNA, Fungal - analysis Double-stranded RNA Fundamental and applied biological sciences. Psychology Histone 4 Morphology Pathogenicity Pest Control, Biological Phytopathology. Animal pests. Plant and forest protection Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Polymorphism, Single-Stranded Conformational Protozoa. Invertebrates Ribosomal DNA Verticillium lecanii β-Tubulin |
| title | Genetic, morphological, and virulence characterization of the entomopathogenic fungus Verticillium lecanii |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T20%3A05%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic,%20morphological,%20and%20virulence%20characterization%20of%20the%20entomopathogenic%20fungus%20Verticillium%20lecanii&rft.jtitle=Journal%20of%20invertebrate%20pathology&rft.au=Sugimoto,%20Midori&rft.date=2003-03-01&rft.volume=82&rft.issue=3&rft.spage=176&rft.epage=187&rft.pages=176-187&rft.issn=0022-2011&rft.eissn=1096-0805&rft.coden=JIVPAZ&rft_id=info:doi/10.1016/S0022-2011(03)00014-4&rft_dat=%3Cproquest_cross%3E18747034%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18747034&rft_id=info:pmid/12676554&rft_els_id=S0022201103000144&rfr_iscdi=true |