Effect of Sample Preparation, Length of Time, and Sample Size on Quantification of Total Lipids from Bovine Liver

The objective was to evaluate the effect of sample preparation (pulverization under liquid nitrogen, homogenization, or sonication), time length of sonication (0−60 s), shaking in chloroform/methanol solvent (0, 2, 4, or 12 h), incubation in chloroform (0 or 12 h), and drying of extracted lipids at...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2003-04, Vol.51 (8), p.2105-2110
Hauptverfasser:	Ametaj, Burim N, Bobe, Gerd, Lu, Yun, Young, Jerry W, Beitz, Donald C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal productions Animals Biological and medical sciences Cattle Chloroform Fundamental and applied biological sciences. Psychology homogenization Indicators and Reagents lipids Lipids - analysis liver Liver - chemistry Methanol Nitrogen Sensitivity and Specificity Sonication Specimen Handling - methods Terrestrial animal productions Time Factors Vertebrates
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