Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients
It has been suggested that HLA-G antigens may provide tumor cells with an effective immune escape mechanism. So far mostly solid tumors have been analyzed; HLA-G antigen was only exceptionally detected. To further examine HLA-G expression, patients were chosen with different forms of leukemia: AML (...
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| Veröffentlicht in: | Leukemia research 2003-07, Vol.27 (7), p.643-648 |
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| creator | Poláková, Katarı́ na Kŕčová, Martina Kuba, Daniel Russ, Gustáv |
| description | It has been suggested that HLA-G antigens may provide tumor cells with an effective immune escape mechanism. So far mostly solid tumors have been analyzed; HLA-G antigen was only exceptionally detected. To further examine HLA-G expression, patients were chosen with different forms of leukemia: AML (25), CML (4), ALL (9), CLL (8), HCL (2) and NHL (3). Using flow cytometry with three HLA-G specific mAbs (87G, 01G and MEM-G/9), western blotting with two specific mAbs (4H84 and MEM-G/1) and RT-PCR, neither HLA-G antigen nor mRNA for any HLA-G isoform was detected. These results strongly suggest that HLA-G antigen is not expressed in freshly isolated human leukemia cells and therefore is not involved in their escape from immune attack. |
| doi_str_mv | 10.1016/S0145-2126(02)00228-X |
| format | Article |
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So far mostly solid tumors have been analyzed; HLA-G antigen was only exceptionally detected. To further examine HLA-G expression, patients were chosen with different forms of leukemia: AML (25), CML (4), ALL (9), CLL (8), HCL (2) and NHL (3). Using flow cytometry with three HLA-G specific mAbs (87G, 01G and MEM-G/9), western blotting with two specific mAbs (4H84 and MEM-G/1) and RT-PCR, neither HLA-G antigen nor mRNA for any HLA-G isoform was detected. These results strongly suggest that HLA-G antigen is not expressed in freshly isolated human leukemia cells and therefore is not involved in their escape from immune attack.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/S0145-2126(02)00228-X</identifier><identifier>PMID: 12681364</identifier><identifier>CODEN: LEREDD</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Antibodies, Monoclonal ; Biological and medical sciences ; Blotting, Western ; Child ; Child, Preschool ; Female ; Flow Cytometry ; Gene Expression Regulation, Leukemic ; Hematologic and hematopoietic diseases ; Histocompatibility Antigens Class I - genetics ; Histocompatibility Antigens Class I - metabolism ; HLA Antigens - genetics ; HLA Antigens - metabolism ; HLA-G ; HLA-G Antigens ; Human leukemia ; Humans ; Leukemia - metabolism ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Male ; Medical sciences ; Middle Aged ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; RNA, Neoplasm - metabolism ; RT-PCR ; Tumor Cells, Cultured ; Western blotting</subject><ispartof>Leukemia research, 2003-07, Vol.27 (7), p.643-648</ispartof><rights>2003 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-483e8a7100be807c941daef41219303fef556fcb06ad78e719219c915b3857e63</citedby><cites>FETCH-LOGICAL-c391t-483e8a7100be807c941daef41219303fef556fcb06ad78e719219c915b3857e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S014521260200228X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14754003$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12681364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Poláková, Katarı&#x0301;na</creatorcontrib><creatorcontrib>Kŕčová, Martina</creatorcontrib><creatorcontrib>Kuba, Daniel</creatorcontrib><creatorcontrib>Russ, Gustáv</creatorcontrib><title>Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients</title><title>Leukemia research</title><addtitle>Leuk Res</addtitle><description>It has been suggested that HLA-G antigens may provide tumor cells with an effective immune escape mechanism. So far mostly solid tumors have been analyzed; HLA-G antigen was only exceptionally detected. To further examine HLA-G expression, patients were chosen with different forms of leukemia: AML (25), CML (4), ALL (9), CLL (8), HCL (2) and NHL (3). Using flow cytometry with three HLA-G specific mAbs (87G, 01G and MEM-G/9), western blotting with two specific mAbs (4H84 and MEM-G/1) and RT-PCR, neither HLA-G antigen nor mRNA for any HLA-G isoform was detected. These results strongly suggest that HLA-G antigen is not expressed in freshly isolated human leukemia cells and therefore is not involved in their escape from immune attack.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Gene Expression Regulation, Leukemic</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Histocompatibility Antigens Class I - genetics</subject><subject>Histocompatibility Antigens Class I - metabolism</subject><subject>HLA Antigens - genetics</subject><subject>HLA Antigens - metabolism</subject><subject>HLA-G</subject><subject>HLA-G Antigens</subject><subject>Human leukemia</subject><subject>Humans</subject><subject>Leukemia - metabolism</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Neoplasm - metabolism</subject><subject>RT-PCR</subject><subject>Tumor Cells, Cultured</subject><subject>Western blotting</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EokvhEUC-gOCQMhPHiXNCqwraSitxKEi9WV5nDIYkDp4som_fbHdFjz2NNPPNzK9PiNcIZwhYf7wGrHRRYlm_h_IDQFma4uaJWKFpVKGN0k_F6j9yIl4w_wIA3WL7XJwsLYOqrlbiej26_pYjyxTk5WZdXEj6N2VijmmUcZSD6-OP0Y2z_EmDm9OUaI5eeup7liGnQfa0-01DdHJyc6Rx5pfiWXA906tjPRXfv3z-dn5ZbL5eXJ2vN4VXLc5FZRQZ1yDAlgw0vq2wcxQqLLFVoAIFrevgt1C7rjHUYLsMfIt6q4xuqFan4t3h7pTTnx3xbIfI-2BupLRj2yjUta6rBdQH0OfEnCnYKcfB5VuLYPc27b1Nu1dlobT3Nu3Nsvfm-GC3Hah72DrqW4C3R8Cxd33IbvSRH7iq0RWAWrhPB44WHX8jZct-UeWpi5n8bLsUH4lyB6ebkMk</recordid><startdate>20030701</startdate><enddate>20030701</enddate><creator>Poláková, Katarı&#x0301;na</creator><creator>Kŕčová, Martina</creator><creator>Kuba, Daniel</creator><creator>Russ, Gustáv</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030701</creationdate><title>Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients</title><author>Poláková, Katarı&#x0301;na ; Kŕčová, Martina ; Kuba, Daniel ; Russ, Gustáv</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-483e8a7100be807c941daef41219303fef556fcb06ad78e719219c915b3857e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Gene Expression Regulation, Leukemic</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Histocompatibility Antigens Class I - genetics</topic><topic>Histocompatibility Antigens Class I - metabolism</topic><topic>HLA Antigens - genetics</topic><topic>HLA Antigens - metabolism</topic><topic>HLA-G</topic><topic>HLA-G Antigens</topic><topic>Human leukemia</topic><topic>Humans</topic><topic>Leukemia - metabolism</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Neoplasm - metabolism</topic><topic>RT-PCR</topic><topic>Tumor Cells, Cultured</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poláková, Katarı&#x0301;na</creatorcontrib><creatorcontrib>Kŕčová, Martina</creatorcontrib><creatorcontrib>Kuba, Daniel</creatorcontrib><creatorcontrib>Russ, Gustáv</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poláková, Katarı&#x0301;na</au><au>Kŕčová, Martina</au><au>Kuba, Daniel</au><au>Russ, Gustáv</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>27</volume><issue>7</issue><spage>643</spage><epage>648</epage><pages>643-648</pages><issn>0145-2126</issn><eissn>1873-5835</eissn><coden>LEREDD</coden><abstract>It has been suggested that HLA-G antigens may provide tumor cells with an effective immune escape mechanism. So far mostly solid tumors have been analyzed; HLA-G antigen was only exceptionally detected. To further examine HLA-G expression, patients were chosen with different forms of leukemia: AML (25), CML (4), ALL (9), CLL (8), HCL (2) and NHL (3). Using flow cytometry with three HLA-G specific mAbs (87G, 01G and MEM-G/9), western blotting with two specific mAbs (4H84 and MEM-G/1) and RT-PCR, neither HLA-G antigen nor mRNA for any HLA-G isoform was detected. These results strongly suggest that HLA-G antigen is not expressed in freshly isolated human leukemia cells and therefore is not involved in their escape from immune attack.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>12681364</pmid><doi>10.1016/S0145-2126(02)00228-X</doi><tpages>6</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Antibodies, Monoclonal Biological and medical sciences Blotting, Western Child Child, Preschool Female Flow Cytometry Gene Expression Regulation, Leukemic Hematologic and hematopoietic diseases Histocompatibility Antigens Class I - genetics Histocompatibility Antigens Class I - metabolism HLA Antigens - genetics HLA Antigens - metabolism HLA-G HLA-G Antigens Human leukemia Humans Leukemia - metabolism Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Male Medical sciences Middle Aged Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism RNA, Neoplasm - metabolism RT-PCR Tumor Cells, Cultured Western blotting |
| title | Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients |
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