Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase
We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of th...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1992-08, Vol.186 (3), p.1249-1256 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Maury, Georges alaoui, Abdelaziz El Morvan, François Muller, Barbara Imbach, Jean-Louis Goody, Roger S. |
| description | We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of the enzyme using Poly(rA).(dT)
14 as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase. The two methods gave the same relative order of affinitiy. Phosphorothioate oligodeoxyribonucleotides had a much higher affinity than oligo(dC)
14 and it was increased on hybridization. Quantitatively similar results were obtained for S(dC)
14 or its analog with bases in the a-configuration. Of the analogs tested, only S(dC)
14 showed priming activity. |
| doi_str_mv | 10.1016/S0006-291X(05)81540-2 |
| format | Article |
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14 as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase. The two methods gave the same relative order of affinitiy. Phosphorothioate oligodeoxyribonucleotides had a much higher affinity than oligo(dC)
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14 as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase. The two methods gave the same relative order of affinitiy. Phosphorothioate oligodeoxyribonucleotides had a much higher affinity than oligo(dC)
14 and it was increased on hybridization. Quantitatively similar results were obtained for S(dC)
14 or its analog with bases in the a-configuration. Of the analogs tested, only S(dC)
14 showed priming activity.</description><subject>AIDS/HIV</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Antiviral Agents - pharmacology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIV Reverse Transcriptase</subject><subject>human immunodeficiency virus 1</subject><subject>Kinetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Denaturation</subject><subject>Oligodeoxyribonucleotides - pharmacology</subject><subject>Polydeoxyribonucleotides - metabolism</subject><subject>Recombinant Proteins - antagonists & inhibitors</subject><subject>Reverse Transcriptase Inhibitors</subject><subject>Templates, Genetic</subject><subject>Thermodynamics</subject><subject>Thionucleotides</subject><subject>Transferases</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKtvCT6iUA0L0kDJjx058QqhqaaVKVKIgbpbXmbBG2TjYTgX_vm53VY49zWjmmQ-9L2PHCKcIqD5-AwBVc40_P4A86VA2UPMXbIWgS4LQvGSrJ-Q1O0zpNwBio_QBO0DRQav1itlb2s6jzXRa3WxCmjchhrzxoVSqMPpfYVrcSCH7nlLVL_NIf0tiU-WnjV_7HGKqwlBdXv2osYp0RzFRlaOdkot-zjbRG_ZqsGOit_t4xL5fnN-eXdbXX79cnX2-rl3DIded7rge1ly2sneklLBKcGwtilbYXiK20GMHAwkSbadRCnCt0kBq7RTvWnHE3u_2zjH8WShls_XJ0TjaicKSTCtQAnD5LIhK8oYrUUC5A10MKUUazBz91sZ_BsE8eGAePTAPAhuQ5tEDw8vc8f7Ast5S_39qJ3rpv9v3bXJ2HIpazqcnTDa6Ebwp2KcdRkW1O0_RJOdpctT7SC6bPvhnHrkHTwKi-A</recordid><startdate>19920814</startdate><enddate>19920814</enddate><creator>Maury, Georges</creator><creator>alaoui, Abdelaziz El</creator><creator>Morvan, François</creator><creator>Muller, Barbara</creator><creator>Imbach, Jean-Louis</creator><creator>Goody, Roger S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19920814</creationdate><title>Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase</title><author>Maury, Georges ; alaoui, Abdelaziz El ; Morvan, François ; Muller, Barbara ; Imbach, Jean-Louis ; Goody, Roger S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-89829fb2575dce663a63217a1373ad51170d180fe3e37891530c7690e6bc62873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>AIDS/HIV</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Antiviral Agents - pharmacology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIV Reverse Transcriptase</topic><topic>human immunodeficiency virus 1</topic><topic>Kinetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Denaturation</topic><topic>Oligodeoxyribonucleotides - pharmacology</topic><topic>Polydeoxyribonucleotides - metabolism</topic><topic>Recombinant Proteins - antagonists & inhibitors</topic><topic>Reverse Transcriptase Inhibitors</topic><topic>Templates, Genetic</topic><topic>Thermodynamics</topic><topic>Thionucleotides</topic><topic>Transferases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maury, Georges</creatorcontrib><creatorcontrib>alaoui, Abdelaziz El</creatorcontrib><creatorcontrib>Morvan, François</creatorcontrib><creatorcontrib>Muller, Barbara</creatorcontrib><creatorcontrib>Imbach, Jean-Louis</creatorcontrib><creatorcontrib>Goody, Roger S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maury, Georges</au><au>alaoui, Abdelaziz El</au><au>Morvan, François</au><au>Muller, Barbara</au><au>Imbach, Jean-Louis</au><au>Goody, Roger S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1992-08-14</date><risdate>1992</risdate><volume>186</volume><issue>3</issue><spage>1249</spage><epage>1256</epage><pages>1249-1256</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of the enzyme using Poly(rA).(dT)
14 as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase. The two methods gave the same relative order of affinitiy. Phosphorothioate oligodeoxyribonucleotides had a much higher affinity than oligo(dC)
14 and it was increased on hybridization. Quantitatively similar results were obtained for S(dC)
14 or its analog with bases in the a-configuration. Of the analogs tested, only S(dC)
14 showed priming activity.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1380799</pmid><doi>10.1016/S0006-291X(05)81540-2</doi><tpages>8</tpages></addata></record> |
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| subjects | AIDS/HIV Analytical, structural and metabolic biochemistry Antiviral Agents - pharmacology Base Sequence Biological and medical sciences Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology HIV Reverse Transcriptase human immunodeficiency virus 1 Kinetics Molecular Sequence Data Nucleic Acid Denaturation Oligodeoxyribonucleotides - pharmacology Polydeoxyribonucleotides - metabolism Recombinant Proteins - antagonists & inhibitors Reverse Transcriptase Inhibitors Templates, Genetic Thermodynamics Thionucleotides Transferases |
| title | Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase |
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