Role of Extracellular Magnesium in Insulin Secretion from Rat Insulinoma Cells
Abstract Magnesium (Mg2+) is an abundant intracellular cation that participates in the regulation of the intracellular concentration of ATP. In this study, we examined the relationship between insulin secretion and intracellular free Mg2+ ([Mg2+]i) in a rat-insulinoma cell line (RIN m5F), using a fl...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1992-09, Vol.200 (4), p.490-494 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
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| creator | Murakami, M. Ishizuka, J. Sumi, S. Nickols, G. A. Cooper, C. W. Townsend, C. M. Thompson, J. C. |
| description | Abstract
Magnesium (Mg2+) is an abundant intracellular cation that participates in the regulation of the intracellular concentration of ATP. In this study, we examined the relationship between insulin secretion and intracellular free Mg2+ ([Mg2+]i) in a rat-insulinoma cell line (RIN m5F), using a fluorescent dye (Mag-fura-2). KCI, forskolin, and D-glyceraldehyde increased [Mg2+]i and insulin secretion from RIN m5F cells in a dose-dependent fashion. Verapamil, a voltage-dependent Ca2+ channel blocker, inhibited the increase of [Mg2+]i that was evoked by KCI, forskolin, and D-glyceraldehyde. In a Mg2+-free buffer, these agents failed to cause an elevation in [Mg2+]i; however, the insulin response to KCI and forskolin was enhanced, compared with that in the presence of Mg2+ (1.25 mM). Our findings suggest that [Mg2+]i is dependent upon extracellular Mg2+, and the influx through the voltage-dependent Ca2+ channel. Mg2+ may competitively inhibit the voltage-dependent Ca2+ channel, which is known to play a role in insulin secretion. An absence of Mg2+ in the extracellular space may result in enhanced insulin secretion. [Mg2+]i may play a role in insulin secretion from RIN m5F cells. |
| doi_str_mv | 10.3181/00379727-200-43459 |
| format | Article |
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Magnesium (Mg2+) is an abundant intracellular cation that participates in the regulation of the intracellular concentration of ATP. In this study, we examined the relationship between insulin secretion and intracellular free Mg2+ ([Mg2+]i) in a rat-insulinoma cell line (RIN m5F), using a fluorescent dye (Mag-fura-2). KCI, forskolin, and D-glyceraldehyde increased [Mg2+]i and insulin secretion from RIN m5F cells in a dose-dependent fashion. Verapamil, a voltage-dependent Ca2+ channel blocker, inhibited the increase of [Mg2+]i that was evoked by KCI, forskolin, and D-glyceraldehyde. In a Mg2+-free buffer, these agents failed to cause an elevation in [Mg2+]i; however, the insulin response to KCI and forskolin was enhanced, compared with that in the presence of Mg2+ (1.25 mM). Our findings suggest that [Mg2+]i is dependent upon extracellular Mg2+, and the influx through the voltage-dependent Ca2+ channel. Mg2+ may competitively inhibit the voltage-dependent Ca2+ channel, which is known to play a role in insulin secretion. An absence of Mg2+ in the extracellular space may result in enhanced insulin secretion. [Mg2+]i may play a role in insulin secretion from RIN m5F cells.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>EISSN: 1525-1373</identifier><identifier>DOI: 10.3181/00379727-200-43459</identifier><identifier>PMID: 1508939</identifier><identifier>CODEN: PSEBAA</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Biological and medical sciences ; Calcium - metabolism ; Cell Line ; Colforsin - pharmacology ; Glyburide - pharmacology ; Glyceraldehyde - pharmacology ; Insulin - metabolism ; Insulin Secretion ; Insulinoma - metabolism ; Kinetics ; Magnesium - metabolism ; Magnesium - pharmacology ; Medical sciences ; Metabolic diseases ; Pancreatic Neoplasms - metabolism ; Potassium Chloride - pharmacology ; Rats ; Tumor Cells, Cultured ; Verapamil - pharmacology</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1992-09, Vol.200 (4), p.490-494</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-eba7b3119ff5bce9cd8ce6116d4e1ba2606dc678ad061f6ac4b8220ab21f90263</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4351244$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1508939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murakami, M.</creatorcontrib><creatorcontrib>Ishizuka, J.</creatorcontrib><creatorcontrib>Sumi, S.</creatorcontrib><creatorcontrib>Nickols, G. A.</creatorcontrib><creatorcontrib>Cooper, C. W.</creatorcontrib><creatorcontrib>Townsend, C. M.</creatorcontrib><creatorcontrib>Thompson, J. C.</creatorcontrib><title>Role of Extracellular Magnesium in Insulin Secretion from Rat Insulinoma Cells</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Abstract
Magnesium (Mg2+) is an abundant intracellular cation that participates in the regulation of the intracellular concentration of ATP. In this study, we examined the relationship between insulin secretion and intracellular free Mg2+ ([Mg2+]i) in a rat-insulinoma cell line (RIN m5F), using a fluorescent dye (Mag-fura-2). KCI, forskolin, and D-glyceraldehyde increased [Mg2+]i and insulin secretion from RIN m5F cells in a dose-dependent fashion. Verapamil, a voltage-dependent Ca2+ channel blocker, inhibited the increase of [Mg2+]i that was evoked by KCI, forskolin, and D-glyceraldehyde. In a Mg2+-free buffer, these agents failed to cause an elevation in [Mg2+]i; however, the insulin response to KCI and forskolin was enhanced, compared with that in the presence of Mg2+ (1.25 mM). Our findings suggest that [Mg2+]i is dependent upon extracellular Mg2+, and the influx through the voltage-dependent Ca2+ channel. Mg2+ may competitively inhibit the voltage-dependent Ca2+ channel, which is known to play a role in insulin secretion. An absence of Mg2+ in the extracellular space may result in enhanced insulin secretion. [Mg2+]i may play a role in insulin secretion from RIN m5F cells.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cell Line</subject><subject>Colforsin - pharmacology</subject><subject>Glyburide - pharmacology</subject><subject>Glyceraldehyde - pharmacology</subject><subject>Insulin - metabolism</subject><subject>Insulin Secretion</subject><subject>Insulinoma - metabolism</subject><subject>Kinetics</subject><subject>Magnesium - metabolism</subject><subject>Magnesium - pharmacology</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Potassium Chloride - pharmacology</subject><subject>Rats</subject><subject>Tumor Cells, Cultured</subject><subject>Verapamil - pharmacology</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><issn>1525-1373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78AUHoQbxVM0mbNkdZ1g9YFfw4h2maSKVt1qQF_fem7qo3T3N4n3dmeAg5BnrOoYQLSnkhC1akjNI041kut8gMcp6nXEi5TWYTkE7EHtkP4Y1SyAsmdsku5LSUXM7I_aNrTeJssvgYPGrTtmOLPrnD196EZuySpk9u-zC2cT4Z7c3QuD6x3nXJIw4_keswmcduOCQ7FttgjjbzgLxcLZ7nN-ny4fp2frlMNRfFkJoKi4oDSGvzShup61IbASDqzECFTFBRa1GUWFMBVqDOqpIxihUDKykT_ICcrfeuvHsfTRhU14Tpe-yNG4MqOGQlAI8gW4PauxC8sWrlmw79pwKqJonqR6KKEtW3xFg62Wwfq87Uf5W1tZifbnIMGlvrsddN-MUyngPLsohdrLGAr0a9udH30cl_h78AF9GHKw</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Murakami, M.</creator><creator>Ishizuka, J.</creator><creator>Sumi, S.</creator><creator>Nickols, G. A.</creator><creator>Cooper, C. W.</creator><creator>Townsend, C. M.</creator><creator>Thompson, J. C.</creator><general>SAGE Publications</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920901</creationdate><title>Role of Extracellular Magnesium in Insulin Secretion from Rat Insulinoma Cells</title><author>Murakami, M. ; Ishizuka, J. ; Sumi, S. ; Nickols, G. A. ; Cooper, C. W. ; Townsend, C. M. ; Thompson, J. C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-eba7b3119ff5bce9cd8ce6116d4e1ba2606dc678ad061f6ac4b8220ab21f90263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Cell Line</topic><topic>Colforsin - pharmacology</topic><topic>Glyburide - pharmacology</topic><topic>Glyceraldehyde - pharmacology</topic><topic>Insulin - metabolism</topic><topic>Insulin Secretion</topic><topic>Insulinoma - metabolism</topic><topic>Kinetics</topic><topic>Magnesium - metabolism</topic><topic>Magnesium - pharmacology</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Potassium Chloride - pharmacology</topic><topic>Rats</topic><topic>Tumor Cells, Cultured</topic><topic>Verapamil - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murakami, M.</creatorcontrib><creatorcontrib>Ishizuka, J.</creatorcontrib><creatorcontrib>Sumi, S.</creatorcontrib><creatorcontrib>Nickols, G. A.</creatorcontrib><creatorcontrib>Cooper, C. W.</creatorcontrib><creatorcontrib>Townsend, C. M.</creatorcontrib><creatorcontrib>Thompson, J. C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murakami, M.</au><au>Ishizuka, J.</au><au>Sumi, S.</au><au>Nickols, G. A.</au><au>Cooper, C. W.</au><au>Townsend, C. M.</au><au>Thompson, J. C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Extracellular Magnesium in Insulin Secretion from Rat Insulinoma Cells</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>200</volume><issue>4</issue><spage>490</spage><epage>494</epage><pages>490-494</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><eissn>1525-1373</eissn><coden>PSEBAA</coden><abstract>Abstract
Magnesium (Mg2+) is an abundant intracellular cation that participates in the regulation of the intracellular concentration of ATP. In this study, we examined the relationship between insulin secretion and intracellular free Mg2+ ([Mg2+]i) in a rat-insulinoma cell line (RIN m5F), using a fluorescent dye (Mag-fura-2). KCI, forskolin, and D-glyceraldehyde increased [Mg2+]i and insulin secretion from RIN m5F cells in a dose-dependent fashion. Verapamil, a voltage-dependent Ca2+ channel blocker, inhibited the increase of [Mg2+]i that was evoked by KCI, forskolin, and D-glyceraldehyde. In a Mg2+-free buffer, these agents failed to cause an elevation in [Mg2+]i; however, the insulin response to KCI and forskolin was enhanced, compared with that in the presence of Mg2+ (1.25 mM). Our findings suggest that [Mg2+]i is dependent upon extracellular Mg2+, and the influx through the voltage-dependent Ca2+ channel. Mg2+ may competitively inhibit the voltage-dependent Ca2+ channel, which is known to play a role in insulin secretion. An absence of Mg2+ in the extracellular space may result in enhanced insulin secretion. [Mg2+]i may play a role in insulin secretion from RIN m5F cells.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>1508939</pmid><doi>10.3181/00379727-200-43459</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Calcium - metabolism Cell Line Colforsin - pharmacology Glyburide - pharmacology Glyceraldehyde - pharmacology Insulin - metabolism Insulin Secretion Insulinoma - metabolism Kinetics Magnesium - metabolism Magnesium - pharmacology Medical sciences Metabolic diseases Pancreatic Neoplasms - metabolism Potassium Chloride - pharmacology Rats Tumor Cells, Cultured Verapamil - pharmacology |
| title | Role of Extracellular Magnesium in Insulin Secretion from Rat Insulinoma Cells |
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