Investigating the regulation of one-carbon metabolism in Arabidopsis thaliana

Serine (Ser) biosynthesis in C3 plants can occur via several pathways. One major route involves the tetrahydrofolate (THF)-dependent activities of the glycine decarboxylase complex (GDC, EC 2.1.1.10) and serine hydroxymethyltransferase (SHMT, EC 2.1.2.1) with glycine (Gly) as one-carbon (1-C) source...

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Veröffentlicht in:	Plant and cell physiology 2003-03, Vol.44 (3), p.233-241
Hauptverfasser:	Li, R. (University of Saskatchewan (Canada)), Moore, M, King, J
Format:	Artikel
Sprache:	eng
Schlagworte:	1-C Abbreviation: D2O Aminohydrolases - metabolism Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis - metabolism ARABIDOPSIS THALIANA BIOSYNTHESIS C3 PLANTS Carbon - metabolism CARBON CYCLE Carbon Dioxide - metabolism Carbon Isotopes deuterium oxide DNA FDH formate dehydrogenase Formate Dehydrogenases - genetics Formate Dehydrogenases - metabolism Formate-Tetrahydrofolate Ligase - metabolism Formates - metabolism gas chromatography-mass spectrometry GC-MS GDC Gene Expression Regulation, Enzymologic GENOMES Gly glycine Glycine - metabolism glycine decarboxylase complex Glycine Hydroxymethyltransferase - metabolism Homocysteine S-Methyltransferase ion chromatography Keywords: One-carbon metabolism — Formate dehydrogenase — Glycine decarboxylase complex — Glycine decarboxylase/serine hydroxymethyltransferase pathway — C1-tetrahydrofolate synthase/serine hydroxymethyltransferase pathway — Arabidopsis thaliana (transgenic and mutant) Magnetic Resonance Spectroscopy Methylenetetrahydrofolate Dehydrogenase (NADP) - metabolism Methyltransferases - metabolism Multienzyme Complexes - metabolism Mutation NMR nuclear magnetic resonance one-carbon Plant Shoots - metabolism Plants, Genetically Modified Ser SERINE Serine - biosynthesis serine hydroxymethyltransferase SHMT tetrahydrofolate THF TRANSGENIC PLANTS wild-type
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