Phospholipids in Trypanosoma cruzi : phosphoinositide composition and turnover
The polyphosphoinositides from Trypanosoma cruzi were isolated by preparative thin-layer chromatography (TLC) and identified. When myo-[3H]inositol was present in the culture medium for five days, analyses showed the presence of phosphatidylinositol (PI), lysophosphatidylinositol (lysoPI), phosphati...
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| Veröffentlicht in: | Lipids 1992-04, Vol.27 (4), p.275-278 |
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| creator | RACAGNI, G GARCIA DE LEMA, M DOMENECH, C. E MACHADO DE DOMENECH, E. E |
| description | The polyphosphoinositides from Trypanosoma cruzi were isolated by preparative thin-layer chromatography (TLC) and identified. When myo-[3H]inositol was present in the culture medium for five days, analyses showed the presence of phosphatidylinositol (PI), lysophosphatidylinositol (lysoPI), phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2). Short-term incubation with 32Pi led to higher percentages of incorporation into phosphatidylethanolamine (PE), lysophosphatidylethanolamine (lysoPE) and PI compared to the other glycerophospholipids. The phosphoinositides (PI, PIP and PIP2) contained a larger proportion of unsaturated than saturated fatty acids. High proportions of 18:2 were found in the three phosphoinositides analyzed, whereas the major saturated fatty acid was 18:0. Water-soluble inositol phosphates (IP, IP2 and IP3) were also identified. |
| doi_str_mv | 10.1007/BF02536475 |
| format | Article |
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Water-soluble inositol phosphates (IP, IP2 and IP3) were also identified.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/BF02536475</identifier><identifier>PMID: 1325592</identifier><identifier>CODEN: LPDSAP</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Animals ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Biotransformation ; Chromatography, Gas ; Chromatography, Thin Layer ; Fundamental and applied biological sciences. 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E</creatorcontrib><creatorcontrib>MACHADO DE DOMENECH, E. E</creatorcontrib><title>Phospholipids in Trypanosoma cruzi : phosphoinositide composition and turnover</title><title>Lipids</title><addtitle>Lipids</addtitle><description>The polyphosphoinositides from Trypanosoma cruzi were isolated by preparative thin-layer chromatography (TLC) and identified. When myo-[3H]inositol was present in the culture medium for five days, analyses showed the presence of phosphatidylinositol (PI), lysophosphatidylinositol (lysoPI), phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2). Short-term incubation with 32Pi led to higher percentages of incorporation into phosphatidylethanolamine (PE), lysophosphatidylethanolamine (lysoPE) and PI compared to the other glycerophospholipids. The phosphoinositides (PI, PIP and PIP2) contained a larger proportion of unsaturated than saturated fatty acids. High proportions of 18:2 were found in the three phosphoinositides analyzed, whereas the major saturated fatty acid was 18:0. Water-soluble inositol phosphates (IP, IP2 and IP3) were also identified.</description><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Biotransformation</subject><subject>Chromatography, Gas</subject><subject>Chromatography, Thin Layer</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Invertebrates</subject><subject>Phosphatidylinositols - analysis</subject><subject>Phosphatidylinositols - pharmacokinetics</subject><subject>Phosphorus Radioisotopes</subject><subject>Protozoa</subject><subject>Tritium</subject><subject>Trypanosoma cruzi - metabolism</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1LxDAQxYMo67p68S7kIB6E6kyT9MObLq4Ki3pYzyVNUjbSNjVphfWvt7qLnmbmvR-P4RFyinCFAOn13QJiwRKeij0yRSGyKGeQ7pMpQMwjHgMekqMQ3scTeS4mZIIsFiKPp-T5de1Ct3a17awO1LZ05TedbF1wjaTKD1-W3tBuC9lRtr3VhirXdL-7a6lsNe0H37pP44_JQSXrYE52c0beFver-WO0fHl4mt8uI8UQ-6isZCkSlul8_ILppKxUppAbTASDzHCeAgIDLiqtscpRw6iqPEamdQbcsBm52OZ23n0MJvRFY4MydS1b44ZQpAw5j5NsBC-3oPIuBG-qovO2kX5TIBQ_5RX_5Y3w2S51KBuj_9FtW6N_vvNlULKuvGyVDX-YYBnkjLNvmy52Yw</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>RACAGNI, G</creator><creator>GARCIA DE LEMA, M</creator><creator>DOMENECH, C. 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Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Biotransformation</topic><topic>Chromatography, Gas</topic><topic>Chromatography, Thin Layer</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Invertebrates</topic><topic>Phosphatidylinositols - analysis</topic><topic>Phosphatidylinositols - pharmacokinetics</topic><topic>Phosphorus Radioisotopes</topic><topic>Protozoa</topic><topic>Tritium</topic><topic>Trypanosoma cruzi - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RACAGNI, G</creatorcontrib><creatorcontrib>GARCIA DE LEMA, M</creatorcontrib><creatorcontrib>DOMENECH, C. E</creatorcontrib><creatorcontrib>MACHADO DE DOMENECH, E. 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E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phospholipids in Trypanosoma cruzi : phosphoinositide composition and turnover</atitle><jtitle>Lipids</jtitle><addtitle>Lipids</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>27</volume><issue>4</issue><spage>275</spage><epage>278</epage><pages>275-278</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><coden>LPDSAP</coden><abstract>The polyphosphoinositides from Trypanosoma cruzi were isolated by preparative thin-layer chromatography (TLC) and identified. When myo-[3H]inositol was present in the culture medium for five days, analyses showed the presence of phosphatidylinositol (PI), lysophosphatidylinositol (lysoPI), phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2). Short-term incubation with 32Pi led to higher percentages of incorporation into phosphatidylethanolamine (PE), lysophosphatidylethanolamine (lysoPE) and PI compared to the other glycerophospholipids. The phosphoinositides (PI, PIP and PIP2) contained a larger proportion of unsaturated than saturated fatty acids. High proportions of 18:2 were found in the three phosphoinositides analyzed, whereas the major saturated fatty acid was 18:0. Water-soluble inositol phosphates (IP, IP2 and IP3) were also identified.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>1325592</pmid><doi>10.1007/BF02536475</doi><tpages>4</tpages></addata></record> |
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| ispartof | Lipids, 1992-04, Vol.27 (4), p.275-278 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Biotransformation Chromatography, Gas Chromatography, Thin Layer Fundamental and applied biological sciences. Psychology Invertebrates Phosphatidylinositols - analysis Phosphatidylinositols - pharmacokinetics Phosphorus Radioisotopes Protozoa Tritium Trypanosoma cruzi - metabolism |
| title | Phospholipids in Trypanosoma cruzi : phosphoinositide composition and turnover |
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