Detection of infectious bronchitis virus by multiplex polymerase chain reaction and sequence analysis

A multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to amplify the S1 and S2 genes of vaccine and recent Taiwanese isolates of infectious bronchitis virus (IBV). DNA fragments of 228 and 400 base pairs in length were amplified among IBV isolates in multiplex PCR, sugge...

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Veröffentlicht in:	Journal of virological methods 2003-04, Vol.109 (1), p.31-37
Hauptverfasser:	Liu, Hung J., Lee, Long H., Shih, Wen L., Lin, Maw Y., Liao, Ming H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animal productions Animals Biological and medical sciences Chickens Cloning, Molecular Coronavirus Infections - veterinary Coronavirus Infections - virology Fundamental and applied biological sciences. Psychology Genetic Variation Infectious bronchitis virus Infectious bronchitis virus - classification Infectious bronchitis virus - genetics Infectious bronchitis virus - isolation & purification Membrane Glycoproteins - genetics Microbiology Multiplex polymerase chain reaction Phylogeny Poultry Diseases - virology Reverse Transcriptase Polymerase Chain Reaction Sequence analysis Sequence Analysis, DNA Spike Glycoprotein, Coronavirus Taiwan Techniques used in virology Terrestrial animal productions Vertebrates Viral Envelope Proteins - genetics Virology
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