Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay, and bacterial culture for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces
Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD 57007, USA. In this study, 5 combinations of 2 DNA extractions and 3 polymerase chain reaction (PCR) techniques were compared with culture for the detection of Mycobacterium paratuberculosis directly from...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2003-03, Vol.15 (2), p.87-93 |
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| container_title | Journal of veterinary diagnostic investigation |
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| creator | Christopher-Hennings, J Dammen, MA Weeks, , SR Epperson, WB Singh, SN Steinlicht, GL Fang, Y Skaare, JL Larsen, JL Payeur, JB Nelson, EA |
| description | Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD 57007, USA.
In this study, 5 combinations of 2 DNA extractions and 3 polymerase chain reaction (PCR) techniques were compared with culture for the detection of Mycobacterium paratuberculosis directly from bovine feces. These combinations included a new commercial extraction technique combined with a commercial PCR/Southern blot technique, nested PCR (nPCR), or real-time PCR, and a university-developed extraction combined with nPCR or real-time PCR. Four of the 5 combinations had statistically similar sensitivities between 93% and 100% and specificity between 95% and 100%, when compared with culture results from 63 bovine fecal samples. These results indicated that using a commercial extraction with a commercial PCR/Southern blot, nPCR, or real-time PCR, or a university-developed extraction with real-time PCR would result in similar sensitivities to culture for the identification of M. paratuberculosis from bovine feces and are valid alternatives to culture. |
| doi_str_mv | 10.1177/104063870301500201 |
| format | Article |
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In this study, 5 combinations of 2 DNA extractions and 3 polymerase chain reaction (PCR) techniques were compared with culture for the detection of Mycobacterium paratuberculosis directly from bovine feces. These combinations included a new commercial extraction technique combined with a commercial PCR/Southern blot technique, nested PCR (nPCR), or real-time PCR, and a university-developed extraction combined with nPCR or real-time PCR. Four of the 5 combinations had statistically similar sensitivities between 93% and 100% and specificity between 95% and 100%, when compared with culture results from 63 bovine fecal samples. These results indicated that using a commercial extraction with a commercial PCR/Southern blot, nPCR, or real-time PCR, or a university-developed extraction with real-time PCR would result in similar sensitivities to culture for the identification of M. paratuberculosis from bovine feces and are valid alternatives to culture.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063870301500201</identifier><identifier>PMID: 12661717</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>Animal bacterial diseases ; Animals ; Bacterial diseases ; Bacteriological Techniques - methods ; Biological and medical sciences ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - microbiology ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Feces - microbiology ; Infectious diseases ; Medical sciences ; Mycobacterium avium subsp. paratuberculosis - isolation & purification ; Paratuberculosis - diagnosis ; Paratuberculosis - microbiology ; Polymerase Chain Reaction - methods ; Sensitivity and Specificity</subject><ispartof>Journal of veterinary diagnostic investigation, 2003-03, Vol.15 (2), p.87-93</ispartof><rights>2003 American Association of Veterinary Laboratory Diagnosticians</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-aff73d263ec4856b524437ba64153a03745c487fa3c2b9450fbe37ca154c9c7d3</citedby><cites>FETCH-LOGICAL-c443t-aff73d263ec4856b524437ba64153a03745c487fa3c2b9450fbe37ca154c9c7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/104063870301500201$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/104063870301500201$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21818,27923,27924,43620,43621</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14607206$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12661717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Christopher-Hennings, J</creatorcontrib><creatorcontrib>Dammen, MA</creatorcontrib><creatorcontrib>Weeks, , SR</creatorcontrib><creatorcontrib>Epperson, WB</creatorcontrib><creatorcontrib>Singh, SN</creatorcontrib><creatorcontrib>Steinlicht, GL</creatorcontrib><creatorcontrib>Fang, Y</creatorcontrib><creatorcontrib>Skaare, JL</creatorcontrib><creatorcontrib>Larsen, JL</creatorcontrib><creatorcontrib>Payeur, JB</creatorcontrib><creatorcontrib>Nelson, EA</creatorcontrib><title>Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay, and bacterial culture for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD 57007, USA.
In this study, 5 combinations of 2 DNA extractions and 3 polymerase chain reaction (PCR) techniques were compared with culture for the detection of Mycobacterium paratuberculosis directly from bovine feces. These combinations included a new commercial extraction technique combined with a commercial PCR/Southern blot technique, nested PCR (nPCR), or real-time PCR, and a university-developed extraction combined with nPCR or real-time PCR. Four of the 5 combinations had statistically similar sensitivities between 93% and 100% and specificity between 95% and 100%, when compared with culture results from 63 bovine fecal samples. These results indicated that using a commercial extraction with a commercial PCR/Southern blot, nPCR, or real-time PCR, or a university-developed extraction with real-time PCR would result in similar sensitivities to culture for the identification of M. paratuberculosis from bovine feces and are valid alternatives to culture.</description><subject>Animal bacterial diseases</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Bacteriological Techniques - methods</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - microbiology</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Feces - microbiology</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mycobacterium avium subsp. paratuberculosis - isolation & purification</subject><subject>Paratuberculosis - diagnosis</subject><subject>Paratuberculosis - microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kclu1EAQhi0EIiHwAhxQX-AUJ726M8doWKWwCMG5VW6Xkx7Z7qHLnmGejlejnbGUAxKXXqq-_69SVVG8FPxCCGsvBde8UleWKy4M55KLR8WpWGlV6pWqHud3BsqZOCmeEW04N9JY8bQ4EbKqhBX2tPizjv0WUqA4sNiycR_Z2y_XDH-PCfwY4kAMhoYNSCM27Nv6-zlLCF05hh6PX8jJPfOx7zH5AN0cZUAEh_N7aZ19MM0JP3XjlJC1MbEGR7z3n6t-Pvi4YFPPYDefNNW0vWC5NxinOltPXaRALAysjrswZBv0SM-LJy10hC-W-6z4-f7dj_XH8ubrh0_r65vSa63GEtrWqkZWCr2-MlVtZA7bGiotjAKurDY5YVtQXtYrbXhbo7IehNF-5W2jzoo3R99tir-mPA3XB_LYdTBgnMhZJbSUpsqgPII-RaKErdum0EM6OMHdvDb379qy6NXiPtU9Ng-SZU8ZeL0AQB66NsHgAz1wuuJW8rn65ZEjuEW3iVMa8lT-X3pxvgu3d_uQ0FEPXZcbkW6za4IwTrorq_4Cw527pg</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Christopher-Hennings, J</creator><creator>Dammen, MA</creator><creator>Weeks, , SR</creator><creator>Epperson, WB</creator><creator>Singh, SN</creator><creator>Steinlicht, GL</creator><creator>Fang, Y</creator><creator>Skaare, JL</creator><creator>Larsen, JL</creator><creator>Payeur, JB</creator><creator>Nelson, EA</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><general>American Association of Veterinary Laboratory Diagnosticians</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay, and bacterial culture for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces</title><author>Christopher-Hennings, J ; Dammen, MA ; Weeks, , SR ; Epperson, WB ; Singh, SN ; Steinlicht, GL ; Fang, Y ; Skaare, JL ; Larsen, JL ; Payeur, JB ; Nelson, EA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-aff73d263ec4856b524437ba64153a03745c487fa3c2b9450fbe37ca154c9c7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animal bacterial diseases</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Bacteriological Techniques - methods</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cattle Diseases - diagnosis</topic><topic>Cattle Diseases - microbiology</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Feces - microbiology</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Mycobacterium avium subsp. paratuberculosis - isolation & purification</topic><topic>Paratuberculosis - diagnosis</topic><topic>Paratuberculosis - microbiology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Christopher-Hennings, J</creatorcontrib><creatorcontrib>Dammen, MA</creatorcontrib><creatorcontrib>Weeks, , SR</creatorcontrib><creatorcontrib>Epperson, WB</creatorcontrib><creatorcontrib>Singh, SN</creatorcontrib><creatorcontrib>Steinlicht, GL</creatorcontrib><creatorcontrib>Fang, Y</creatorcontrib><creatorcontrib>Skaare, JL</creatorcontrib><creatorcontrib>Larsen, JL</creatorcontrib><creatorcontrib>Payeur, JB</creatorcontrib><creatorcontrib>Nelson, EA</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Christopher-Hennings, J</au><au>Dammen, MA</au><au>Weeks, , SR</au><au>Epperson, WB</au><au>Singh, SN</au><au>Steinlicht, GL</au><au>Fang, Y</au><au>Skaare, JL</au><au>Larsen, JL</au><au>Payeur, JB</au><au>Nelson, EA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay, and bacterial culture for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>15</volume><issue>2</issue><spage>87</spage><epage>93</epage><pages>87-93</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD 57007, USA.
In this study, 5 combinations of 2 DNA extractions and 3 polymerase chain reaction (PCR) techniques were compared with culture for the detection of Mycobacterium paratuberculosis directly from bovine feces. These combinations included a new commercial extraction technique combined with a commercial PCR/Southern blot technique, nested PCR (nPCR), or real-time PCR, and a university-developed extraction combined with nPCR or real-time PCR. Four of the 5 combinations had statistically similar sensitivities between 93% and 100% and specificity between 95% and 100%, when compared with culture results from 63 bovine fecal samples. These results indicated that using a commercial extraction with a commercial PCR/Southern blot, nPCR, or real-time PCR, or a university-developed extraction with real-time PCR would result in similar sensitivities to culture for the identification of M. paratuberculosis from bovine feces and are valid alternatives to culture.</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>12661717</pmid><doi>10.1177/104063870301500201</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; SAGE Complete A-Z List; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animal bacterial diseases Animals Bacterial diseases Bacteriological Techniques - methods Biological and medical sciences Cattle Cattle Diseases - diagnosis Cattle Diseases - microbiology DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Feces - microbiology Infectious diseases Medical sciences Mycobacterium avium subsp. paratuberculosis - isolation & purification Paratuberculosis - diagnosis Paratuberculosis - microbiology Polymerase Chain Reaction - methods Sensitivity and Specificity |
| title | Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay, and bacterial culture for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces |
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