Analysis of the Frequencies and of the Memory T Cell Phenotypes of Human CD8+ T Cells Specific for Influenza A Viruses
We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry–based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility com...
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| Veröffentlicht in: | The Journal of infectious diseases 2003-04, Vol.187 (7), p.1075-1084 |
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| container_title | The Journal of infectious diseases |
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| creator | Xiao-Song He Mahmood, Kutubuddin Maecker, Holden T. Holmes, Tyson H. Kemble, George W. Arvin, Ann M. Greenberg, Harry B. |
| description | We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry–based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I–restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus–specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I–restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus– and cytomegalovirus-specific CD8+ T cells suggest that influenza virus–specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis |
| doi_str_mv | 10.1086/368218 |
| format | Article |
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Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I–restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus–specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I–restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus– and cytomegalovirus-specific CD8+ T cells suggest that influenza virus–specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/368218</identifier><identifier>PMID: 12660922</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adult ; Allantoic fluid ; Antigens ; Antigens, Viral - immunology ; Biological and medical sciences ; CD8-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - metabolism ; Cell Division ; Cytomegalovirus ; Epitopes ; Female ; Flow Cytometry ; Fundamental and applied biological sciences. 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Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I–restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus–specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I–restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus– and cytomegalovirus-specific CD8+ T cells suggest that influenza virus–specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis</description><subject>Adult</subject><subject>Allantoic fluid</subject><subject>Antigens</subject><subject>Antigens, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cell Division</subject><subject>Cytomegalovirus</subject><subject>Epitopes</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Histocompatibility Antigens Class I - immunology</subject><subject>Humans</subject><subject>Immunologic Memory</subject><subject>Infections</subject><subject>Influenza A virus</subject><subject>Influenza A virus - immunology</subject><subject>Interferon-gamma - metabolism</subject><subject>Lymphocyte Count</subject><subject>Male</subject><subject>Memory</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Orthomyxoviridae</subject><subject>Phenotype</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>T lymphocytes</subject><subject>Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology</subject><subject>Tumor Necrosis Factor Receptor Superfamily, Member 7 - metabolism</subject><subject>Virology</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV1rFDEYhYModq36D5Qo6I2M5mMmH5fLrnULLQpdRbwJmUxCZ52ZjMlM6fbXN3amXRC8CuQ8OXnfcwB4idFHjAT7RJkgWDwCC1xQnjGG6WOwQIiQDAspj8CzGHcIoZwy_hQcYcIYkoQswNWy080-1hF6B4dLC0-C_TPaztQ2Qt1V99fntvVhD7dwZZsGfru0nR_2vb17thlb3cHVWnyY9QgvemtqVxvofICnnWuS5Y2GS_ijDmO08Tl44nQT7Yv5PAbfTz5vV5vs7OuX09XyLDN5gYZMOp1LiQ3JNTJGi1LQUiCssbG4IoyQkgtBqLGII-oYI67ioqSYOl5xRAw9Bu8n3z74tFYcVFtHk0bUnfVjVJziHGEsEvj2H3Dnx5CyiYoQKiRNiR3cTPAxButUH-pWh73CSP2tQU01JPD17DaWra0O2Jx7At7NgI5GNy7olHg8cDknkuYycW8mzo_9_z97NTG7OPjwQFGEREFlkfRs0us42OsHXYffinHKC7X5-Uutt3K9OWdrdUFvAYlUrtA</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Xiao-Song He</creator><creator>Mahmood, Kutubuddin</creator><creator>Maecker, Holden T.</creator><creator>Holmes, Tyson H.</creator><creator>Kemble, George W.</creator><creator>Arvin, Ann M.</creator><creator>Greenberg, Harry B.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Analysis of the Frequencies and of the Memory T Cell Phenotypes of Human CD8+ T Cells Specific for Influenza A Viruses</title><author>Xiao-Song He ; Mahmood, Kutubuddin ; Maecker, Holden T. ; Holmes, Tyson H. ; Kemble, George W. ; Arvin, Ann M. ; Greenberg, Harry B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-9fa4991c24a0cca8b83b801a1ce1d2622b78823ce0703f662fd78b313f7d702c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Allantoic fluid</topic><topic>Antigens</topic><topic>Antigens, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Cell Division</topic><topic>Cytomegalovirus</topic><topic>Epitopes</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Histocompatibility Antigens Class I - immunology</topic><topic>Humans</topic><topic>Immunologic Memory</topic><topic>Infections</topic><topic>Influenza A virus</topic><topic>Influenza A virus - immunology</topic><topic>Interferon-gamma - metabolism</topic><topic>Lymphocyte Count</topic><topic>Male</topic><topic>Memory</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Orthomyxoviridae</topic><topic>Phenotype</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>T lymphocytes</topic><topic>Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology</topic><topic>Tumor Necrosis Factor Receptor Superfamily, Member 7 - metabolism</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiao-Song He</creatorcontrib><creatorcontrib>Mahmood, Kutubuddin</creatorcontrib><creatorcontrib>Maecker, Holden T.</creatorcontrib><creatorcontrib>Holmes, Tyson H.</creatorcontrib><creatorcontrib>Kemble, George W.</creatorcontrib><creatorcontrib>Arvin, Ann M.</creatorcontrib><creatorcontrib>Greenberg, Harry B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiao-Song He</au><au>Mahmood, Kutubuddin</au><au>Maecker, Holden T.</au><au>Holmes, Tyson H.</au><au>Kemble, George W.</au><au>Arvin, Ann M.</au><au>Greenberg, Harry B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the Frequencies and of the Memory T Cell Phenotypes of Human CD8+ T Cells Specific for Influenza A Viruses</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>The Journal of Infectious Diseases</stitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>187</volume><issue>7</issue><spage>1075</spage><epage>1084</epage><pages>1075-1084</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry–based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I–restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus–specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I–restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus– and cytomegalovirus-specific CD8+ T cells suggest that influenza virus–specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>12660922</pmid><doi>10.1086/368218</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of infectious diseases, 2003-04, Vol.187 (7), p.1075-1084 |
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| source | MEDLINE; Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adult Allantoic fluid Antigens Antigens, Viral - immunology Biological and medical sciences CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - metabolism Cell Division Cytomegalovirus Epitopes Female Flow Cytometry Fundamental and applied biological sciences. Psychology Gene Expression Regulation Histocompatibility Antigens Class I - immunology Humans Immunologic Memory Infections Influenza A virus Influenza A virus - immunology Interferon-gamma - metabolism Lymphocyte Count Male Memory Microbiology Middle Aged Orthomyxoviridae Phenotype Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains T lymphocytes Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology Tumor Necrosis Factor Receptor Superfamily, Member 7 - metabolism Virology Viruses |
| title | Analysis of the Frequencies and of the Memory T Cell Phenotypes of Human CD8+ T Cells Specific for Influenza A Viruses |
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