The shielding effect of glycerol against protein ionization in electrospray mass spectrometry

Most commercial recombinant proteins used as molecular biology tools, as well as many academically made preparations, are generally maintained in the presence of high glycerol concentrations after purification to maintain their biological activity. The present study shows that larger proteins contai...

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Veröffentlicht in:	Rapid communications in mass spectrometry 2003-01, Vol.17 (7), p.672-677
Hauptverfasser:	Mendes, Maria Anita, Chies, Jocelei Maria, Dias, Ana Christina de Oliveira, Filho, Spartacos Astofi, Palma, Mario Sergio
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Enzyme Stability Glycerol - chemistry Horses Ions - chemistry Myoglobin - chemistry Recombinant Proteins - chemistry Spectrometry, Mass, Electrospray Ionization - methods Taq Polymerase - chemistry Thermus
Online-Zugang:	Volltext
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