A new strategy for backbone resonance assignment in large proteins using a MQ-HACACO experiment

A new strategy of backbone resonance assignment is proposed based on a combination of the most sensitive TROSY-type triple resonance experiments such as TROSY-HNCA and TROSY-HNCO with a new 3D multiple-quantum HACACO experiment. The favourable relaxation properties of the multiple-quantum coherences...

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Veröffentlicht in:	Journal of biomolecular NMR 2003-02, Vol.25 (2), p.147-152
Hauptverfasser:	Pervushin, Konstantin, Eletsky, Alexander
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis - enzymology Carbon Isotopes Chorismate Mutase - chemistry Deuterium Experiments Isotope Labeling Low temperature Magnetic Resonance Spectroscopy - methods Protein Conformation Proteins Proteins - chemistry Resonance Thermodynamics
Online-Zugang:	Volltext
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