Construction of a BAC library and generation of BAC end sequence-tagged connectors for genome sequencing of the African malaria mosquito Anopheles gambiae
A Bacterial Artificial Chromosome (BAC) genomic DNA library of Anopheles gambiae, the major human malaria vector in sub-Saharan Africa, was constructed and characterized. This library (ND-TAM) is composed of 30,720 BAC clones in eighty 384-well plates. The estimated average insert size of the librar...
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| Veröffentlicht in: | Molecular genetics and genomics : MGG 2003-03, Vol.268 (6), p.720-728 |
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| creator | Hong, Y S Hogan, J R Wang, X Sarkar, A Sim, C Loftus, B J Ren, C Huff, E R Carlile, J L Black, K Zhang, H-B Gardner, M J Collins, F H |
| description | A Bacterial Artificial Chromosome (BAC) genomic DNA library of Anopheles gambiae, the major human malaria vector in sub-Saharan Africa, was constructed and characterized. This library (ND-TAM) is composed of 30,720 BAC clones in eighty 384-well plates. The estimated average insert size of the library is 133 kb, with an overall genome coverage of approximately 14-fold. The ends of approximately two-thirds of the clones in the library were sequenced, yielding 32,340 pair-mate ends. A statistical analysis (G-test) of the results of PCR screening of the library indicated a random distribution of BACs in the genome, although one gap encompassing the white locus on the X-chromosome was identified. Furthermore, combined with another previously constructed BAC library (ND-1), ~2,000 BACs have been physically mapped by polytene chromosomal in situ hybridization. These BAC end pair mates and physically mapped BACs have been useful for both the assembly of a fully sequenced A. gambiae genome and for linking the assembled sequence to the three polytene chromosomes. This ND-TAM library is now publicly available at both http://www.malaria.mr4.org/mr4pages/index.html/ and http://hbz.tamu.edu/, providing a valuable resource to the mosquito research community. |
| doi_str_mv | 10.1007/s00438-003-0813-7 |
| format | Article |
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This library (ND-TAM) is composed of 30,720 BAC clones in eighty 384-well plates. The estimated average insert size of the library is 133 kb, with an overall genome coverage of approximately 14-fold. The ends of approximately two-thirds of the clones in the library were sequenced, yielding 32,340 pair-mate ends. A statistical analysis (G-test) of the results of PCR screening of the library indicated a random distribution of BACs in the genome, although one gap encompassing the white locus on the X-chromosome was identified. Furthermore, combined with another previously constructed BAC library (ND-1), ~2,000 BACs have been physically mapped by polytene chromosomal in situ hybridization. These BAC end pair mates and physically mapped BACs have been useful for both the assembly of a fully sequenced A. gambiae genome and for linking the assembled sequence to the three polytene chromosomes. This ND-TAM library is now publicly available at both http://www.malaria.mr4.org/mr4pages/index.html/ and http://hbz.tamu.edu/, providing a valuable resource to the mosquito research community.</description><identifier>ISSN: 1617-4615</identifier><identifier>EISSN: 1617-4623</identifier><identifier>DOI: 10.1007/s00438-003-0813-7</identifier><identifier>PMID: 12655398</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Animals ; Anopheles - genetics ; Anopheles - parasitology ; Chromosomes ; Chromosomes, Artificial, Bacterial - genetics ; Cloning ; Gene Library ; Genes, Insect ; Genome ; Genomes ; Genomics ; Humans ; In Situ Hybridization ; Insect Vectors - genetics ; Insect Vectors - parasitology ; Insecticides ; Malaria ; Malaria - transmission ; Mosquitoes ; Parasites ; Polymerase Chain Reaction ; Sequence Tagged Sites ; Tropical diseases</subject><ispartof>Molecular genetics and genomics : MGG, 2003-03, Vol.268 (6), p.720-728</ispartof><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-e4c1794d569ab9310cbb6d8651e9098e52da05afc8e50f4882f8866bc6d7fb243</citedby><cites>FETCH-LOGICAL-c421t-e4c1794d569ab9310cbb6d8651e9098e52da05afc8e50f4882f8866bc6d7fb243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12655398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hong, Y S</creatorcontrib><creatorcontrib>Hogan, J R</creatorcontrib><creatorcontrib>Wang, X</creatorcontrib><creatorcontrib>Sarkar, A</creatorcontrib><creatorcontrib>Sim, C</creatorcontrib><creatorcontrib>Loftus, B J</creatorcontrib><creatorcontrib>Ren, C</creatorcontrib><creatorcontrib>Huff, E R</creatorcontrib><creatorcontrib>Carlile, J L</creatorcontrib><creatorcontrib>Black, K</creatorcontrib><creatorcontrib>Zhang, H-B</creatorcontrib><creatorcontrib>Gardner, M J</creatorcontrib><creatorcontrib>Collins, F H</creatorcontrib><title>Construction of a BAC library and generation of BAC end sequence-tagged connectors for genome sequencing of the African malaria mosquito Anopheles gambiae</title><title>Molecular genetics and genomics : MGG</title><addtitle>Mol Genet Genomics</addtitle><description>A Bacterial Artificial Chromosome (BAC) genomic DNA library of Anopheles gambiae, the major human malaria vector in sub-Saharan Africa, was constructed and characterized. 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This ND-TAM library is now publicly available at both http://www.malaria.mr4.org/mr4pages/index.html/ and http://hbz.tamu.edu/, providing a valuable resource to the mosquito research community.</description><subject>Animals</subject><subject>Anopheles - genetics</subject><subject>Anopheles - parasitology</subject><subject>Chromosomes</subject><subject>Chromosomes, Artificial, Bacterial - genetics</subject><subject>Cloning</subject><subject>Gene Library</subject><subject>Genes, Insect</subject><subject>Genome</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Insect Vectors - genetics</subject><subject>Insect Vectors - parasitology</subject><subject>Insecticides</subject><subject>Malaria</subject><subject>Malaria - transmission</subject><subject>Mosquitoes</subject><subject>Parasites</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Tagged Sites</subject><subject>Tropical diseases</subject><issn>1617-4615</issn><issn>1617-4623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc1u1TAQhS1ERcstD8AGWSzYBcY_sZ3l5Yo_qRKbdh05ziR1ldi3drLgVXjaOrq3ILFh5ZHnO0czcwh5y-AjA9CfMoAUpgIQFRgmKv2CXDHFdCUVFy__1Ky-JK9zfgBgWnH9ilwyrupaNOaK_D7EkJe0usXHQONALf28P9DJd8mmX9SGno4YMNnn_tbF8pvxccXgsFrsOGJPXQwB3RJTpkNMmyjO-Ez5MG7a5R7pfkje2UBnO9nkLZ1jflz9Euk-xOM9TpjpaOfOW7wmF4OdMr45vzty9_XL7eF7dfPz24_D_qZykrOlQumYbmRfq8Z2jWDguk71RtUMG2gM1ry3UNvBlRIGaQwfjFGqc6rXQ8el2JEPJ99jimXavLSzzw6nyQaMa261YEJw4P8FmdHABKgCvv8HfIhrCmWJYiZlmRaaArET5FLMOeHQHpOfy9FbBu0Wb3uKty3xtlu8Rbsj787Gazdj_1dxzlM8AdckoWc</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Hong, Y S</creator><creator>Hogan, J R</creator><creator>Wang, X</creator><creator>Sarkar, A</creator><creator>Sim, C</creator><creator>Loftus, B J</creator><creator>Ren, C</creator><creator>Huff, E R</creator><creator>Carlile, J L</creator><creator>Black, K</creator><creator>Zhang, H-B</creator><creator>Gardner, M J</creator><creator>Collins, F H</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Construction of a BAC library and generation of BAC end sequence-tagged connectors for genome sequencing of the African malaria mosquito Anopheles gambiae</title><author>Hong, Y S ; Hogan, J R ; Wang, X ; Sarkar, A ; Sim, C ; Loftus, B J ; Ren, C ; Huff, E R ; Carlile, J L ; Black, K ; Zhang, H-B ; Gardner, M J ; Collins, F H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-e4c1794d569ab9310cbb6d8651e9098e52da05afc8e50f4882f8866bc6d7fb243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Anopheles - 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Academic</collection><jtitle>Molecular genetics and genomics : MGG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hong, Y S</au><au>Hogan, J R</au><au>Wang, X</au><au>Sarkar, A</au><au>Sim, C</au><au>Loftus, B J</au><au>Ren, C</au><au>Huff, E R</au><au>Carlile, J L</au><au>Black, K</au><au>Zhang, H-B</au><au>Gardner, M J</au><au>Collins, F H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of a BAC library and generation of BAC end sequence-tagged connectors for genome sequencing of the African malaria mosquito Anopheles gambiae</atitle><jtitle>Molecular genetics and genomics : MGG</jtitle><addtitle>Mol Genet Genomics</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>268</volume><issue>6</issue><spage>720</spage><epage>728</epage><pages>720-728</pages><issn>1617-4615</issn><eissn>1617-4623</eissn><abstract>A Bacterial Artificial Chromosome (BAC) genomic DNA library of Anopheles gambiae, the major human malaria vector in sub-Saharan Africa, was constructed and characterized. This library (ND-TAM) is composed of 30,720 BAC clones in eighty 384-well plates. The estimated average insert size of the library is 133 kb, with an overall genome coverage of approximately 14-fold. The ends of approximately two-thirds of the clones in the library were sequenced, yielding 32,340 pair-mate ends. A statistical analysis (G-test) of the results of PCR screening of the library indicated a random distribution of BACs in the genome, although one gap encompassing the white locus on the X-chromosome was identified. Furthermore, combined with another previously constructed BAC library (ND-1), ~2,000 BACs have been physically mapped by polytene chromosomal in situ hybridization. These BAC end pair mates and physically mapped BACs have been useful for both the assembly of a fully sequenced A. gambiae genome and for linking the assembled sequence to the three polytene chromosomes. This ND-TAM library is now publicly available at both http://www.malaria.mr4.org/mr4pages/index.html/ and http://hbz.tamu.edu/, providing a valuable resource to the mosquito research community.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>12655398</pmid><doi>10.1007/s00438-003-0813-7</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Anopheles - genetics Anopheles - parasitology Chromosomes Chromosomes, Artificial, Bacterial - genetics Cloning Gene Library Genes, Insect Genome Genomes Genomics Humans In Situ Hybridization Insect Vectors - genetics Insect Vectors - parasitology Insecticides Malaria Malaria - transmission Mosquitoes Parasites Polymerase Chain Reaction Sequence Tagged Sites Tropical diseases |
| title | Construction of a BAC library and generation of BAC end sequence-tagged connectors for genome sequencing of the African malaria mosquito Anopheles gambiae |
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