Type-1 ribosome-inactivating protein from iris bulbs: a useful agronomic tool to engineer virus resistance?

To study the in planta antiviral activity of a type-1 ribosome-inactivating protein from iris bulbs, called IRIP, Nicotiana tabacum cv. Samsun NN was transformed with the IRIP sequence expressed under the control of the 35S cauliflower mosaic virus promoter. Molecular analysis of the transgenic plan...

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Veröffentlicht in:	Plant molecular biology 2003-03, Vol.51 (4), p.567-576
Hauptverfasser:	Desmyter, Stijn, Vandenbussche, Frank, Hao, Qiang, Proost, Paul, Peumans, Willy J, Van Damme, Els J M
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture - methods Bioassays Blotting, Northern Cytotoxicity Electrophoresis, Polyacrylamide Gel Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Immunity, Innate - genetics Iris - enzymology N-Glycosyl Hydrolases - genetics N-Glycosyl Hydrolases - metabolism Nicotiana - genetics Nicotiana - virology Plant Diseases - genetics Plant Diseases - virology Plant Proteins - genetics Plant Proteins - metabolism Plant Structures - enzymology Plants, Genetically Modified Proteins Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Ribosome Inactivating Proteins Ribosome Inactivating Proteins, Type 2 RNA, Plant - genetics RNA, Plant - metabolism Tobacco Tobacco Mosaic Virus - growth & development Transgenic plants
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