Identification of a genomic DNA fragment containing the Drosophila melanogaster ovarian tumor gene ( otu) and localization of regions governing its expression
We have identified a genomic DNA fragment which restores fertility to mutants of the ovarian tumor locus ( otu) of Drosophila melanogaster. Germ-line transformants bearing this fragment express otu mRNA with the same tissue specificity as, and at levels comparable to, the wild-type otu gene. Transcr...
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| Veröffentlicht in: | Gene 1992-09, Vol.118 (2), p.171-179 |
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| container_title | Gene |
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| creator | Comer, Allen R. Searles, Lillie L. Kalfayan, Laura J. |
| description | We have identified a genomic DNA fragment which restores fertility to mutants of the ovarian tumor locus (
otu) of
Drosophila melanogaster. Germ-line transformants bearing this fragment express
otu mRNA with the same tissue specificity as, and at levels comparable to, the wild-type
otu gene. Transcription from the
otu promoter,
P
otu, which lacks a TATA element, appears to be initiated at multiple transcription start points (
tsp) within an 80-bp region. Deletion of sequences upstream of the
tsp indicates that a region between nucleotides −190 and −310 is required for proper expression from the
otu gene. A DNA fragment containing 452 bp upstream and 126 bp downstream from the
tsp is able to direct expression of the
Escherichia coli lacZ gene in the germ cells of the ovary and testis, indicating that
cis-acting regulatory elements governing these expression patterns are located in a 578-bp region surrounding the multiple
tsp. |
| doi_str_mv | 10.1016/0378-1119(92)90186-S |
| format | Article |
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otu) of
Drosophila melanogaster. Germ-line transformants bearing this fragment express
otu mRNA with the same tissue specificity as, and at levels comparable to, the wild-type
otu gene. Transcription from the
otu promoter,
P
otu, which lacks a TATA element, appears to be initiated at multiple transcription start points (
tsp) within an 80-bp region. Deletion of sequences upstream of the
tsp indicates that a region between nucleotides −190 and −310 is required for proper expression from the
otu gene. A DNA fragment containing 452 bp upstream and 126 bp downstream from the
tsp is able to direct expression of the
Escherichia coli lacZ gene in the germ cells of the ovary and testis, indicating that
cis-acting regulatory elements governing these expression patterns are located in a 578-bp region surrounding the multiple
tsp.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(92)90186-S</identifier><identifier>PMID: 1511891</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; deletions ; DNA Mutational Analysis ; Drosophila melanogaster ; Drosophila melanogaster - genetics ; Drosophila Proteins ; Female ; Fundamental and applied biological sciences. Psychology ; gene expression ; germ cells ; germ line expression ; Insect Hormones - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; mutagenesis ; Mutation - genetics ; neoplasms ; nucleotide sequences ; oogenesis ; ovaries ; promoter regions ; Promoter Regions, Genetic - genetics ; Recombinant DNA ; Recombinant Fusion Proteins - genetics ; restriction mapping ; RNA, Messenger - genetics ; structural genes ; transcription (genetics) ; transcription start point ; Transcription. Transcription factor. Splicing. Rna processing ; transposons</subject><ispartof>Gene, 1992-09, Vol.118 (2), p.171-179</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-26e7df78e7bc0784074e82fe94b0f48f43de43385ea6eb3c308f4d5eae12330d3</citedby><cites>FETCH-LOGICAL-c441t-26e7df78e7bc0784074e82fe94b0f48f43de43385ea6eb3c308f4d5eae12330d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(92)90186-S$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5496108$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1511891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Comer, Allen R.</creatorcontrib><creatorcontrib>Searles, Lillie L.</creatorcontrib><creatorcontrib>Kalfayan, Laura J.</creatorcontrib><title>Identification of a genomic DNA fragment containing the Drosophila melanogaster ovarian tumor gene ( otu) and localization of regions governing its expression</title><title>Gene</title><addtitle>Gene</addtitle><description>We have identified a genomic DNA fragment which restores fertility to mutants of the ovarian tumor locus (
otu) of
Drosophila melanogaster. Germ-line transformants bearing this fragment express
otu mRNA with the same tissue specificity as, and at levels comparable to, the wild-type
otu gene. Transcription from the
otu promoter,
P
otu, which lacks a TATA element, appears to be initiated at multiple transcription start points (
tsp) within an 80-bp region. Deletion of sequences upstream of the
tsp indicates that a region between nucleotides −190 and −310 is required for proper expression from the
otu gene. A DNA fragment containing 452 bp upstream and 126 bp downstream from the
tsp is able to direct expression of the
Escherichia coli lacZ gene in the germ cells of the ovary and testis, indicating that
cis-acting regulatory elements governing these expression patterns are located in a 578-bp region surrounding the multiple
tsp.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>deletions</subject><subject>DNA Mutational Analysis</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila Proteins</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>germ cells</subject><subject>germ line expression</subject><subject>Insect Hormones - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>mutagenesis</subject><subject>Mutation - genetics</subject><subject>neoplasms</subject><subject>nucleotide sequences</subject><subject>oogenesis</subject><subject>ovaries</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant DNA</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>restriction mapping</subject><subject>RNA, Messenger - genetics</subject><subject>structural genes</subject><subject>transcription (genetics)</subject><subject>transcription start point</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>gene expression</topic><topic>germ cells</topic><topic>germ line expression</topic><topic>Insect Hormones - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>mutagenesis</topic><topic>Mutation - genetics</topic><topic>neoplasms</topic><topic>nucleotide sequences</topic><topic>oogenesis</topic><topic>ovaries</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinant DNA</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>restriction mapping</topic><topic>RNA, Messenger - genetics</topic><topic>structural genes</topic><topic>transcription (genetics)</topic><topic>transcription start point</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Comer, Allen R.</creatorcontrib><creatorcontrib>Searles, Lillie L.</creatorcontrib><creatorcontrib>Kalfayan, Laura J.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Comer, Allen R.</au><au>Searles, Lillie L.</au><au>Kalfayan, Laura J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a genomic DNA fragment containing the Drosophila melanogaster ovarian tumor gene ( otu) and localization of regions governing its expression</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1992-09-10</date><risdate>1992</risdate><volume>118</volume><issue>2</issue><spage>171</spage><epage>179</epage><pages>171-179</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>We have identified a genomic DNA fragment which restores fertility to mutants of the ovarian tumor locus (
otu) of
Drosophila melanogaster. Germ-line transformants bearing this fragment express
otu mRNA with the same tissue specificity as, and at levels comparable to, the wild-type
otu gene. Transcription from the
otu promoter,
P
otu, which lacks a TATA element, appears to be initiated at multiple transcription start points (
tsp) within an 80-bp region. Deletion of sequences upstream of the
tsp indicates that a region between nucleotides −190 and −310 is required for proper expression from the
otu gene. A DNA fragment containing 452 bp upstream and 126 bp downstream from the
tsp is able to direct expression of the
Escherichia coli lacZ gene in the germ cells of the ovary and testis, indicating that
cis-acting regulatory elements governing these expression patterns are located in a 578-bp region surrounding the multiple
tsp.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>1511891</pmid><doi>10.1016/0378-1119(92)90186-S</doi><tpages>9</tpages></addata></record> |
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| ispartof | Gene, 1992-09, Vol.118 (2), p.171-179 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Base Sequence Biological and medical sciences Cloning, Molecular deletions DNA Mutational Analysis Drosophila melanogaster Drosophila melanogaster - genetics Drosophila Proteins Female Fundamental and applied biological sciences. Psychology gene expression germ cells germ line expression Insect Hormones - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data mutagenesis Mutation - genetics neoplasms nucleotide sequences oogenesis ovaries promoter regions Promoter Regions, Genetic - genetics Recombinant DNA Recombinant Fusion Proteins - genetics restriction mapping RNA, Messenger - genetics structural genes transcription (genetics) transcription start point Transcription. Transcription factor. Splicing. Rna processing transposons |
| title | Identification of a genomic DNA fragment containing the Drosophila melanogaster ovarian tumor gene ( otu) and localization of regions governing its expression |
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