Measurement of UDP- N-acetylglucosaminyl transferase (OGT) in brain cytosol and characterization of anti-OGT antibodies

UDP- N-acetylglucosaminyl transferase (OGT) catalyzes O-linked glycosylation of cytosolic and nuclear proteins, but enzyme studies have been hampered by the lack of a rapid, sensitive, and economical OGT assay. Employed assay methods typically involved the use of HPLC, formic acid, and large amounts...

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Veröffentlicht in:	Analytical biochemistry 2003-03, Vol.314 (2), p.169-179
Hauptverfasser:	Marshall, Stephen, Duong, Trung, Orbus, Ryan J, Rumberger, John M, Okuyama, Ryo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - immunology Antibodies - pharmacology Blotting, Western Brain - enzymology Chemical Precipitation Cytosol - drug effects Cytosol - enzymology Electrophoresis, Polyacrylamide Gel Glucosamine - analogs & derivatives Glucosamine - pharmacology Male N-Acetylglucosaminyltransferases - drug effects N-Acetylglucosaminyltransferases - immunology N-Acetylglucosaminyltransferases - metabolism Polyethylene Glycols - chemistry Potassium Chloride - pharmacology Rats Rats, Sprague-Dawley Sodium Chloride - pharmacology Temperature Time Factors Uridine Diphosphate - pharmacology
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