Ultra‐rapid freezing of very low numbers of sperm using cryoloops
BACKGROUND: With the availability of ICSI, men with severe oligozoospermia (
Gespeichert in:
| Veröffentlicht in: | Human reproduction (Oxford) 2003-04, Vol.18 (4), p.788-795 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 795 |
|---|---|
| container_issue | 4 |
| container_start_page | 788 |
| container_title | Human reproduction (Oxford) |
| container_volume | 18 |
| creator | Schuster, Timothy G. Keller, Laura M. Dunn, Rodney L. Ohl, Dana A. Smith, Gary D. |
| description | BACKGROUND: With the availability of ICSI, men with severe oligozoospermia ( |
| doi_str_mv | 10.1093/humrep/deg162 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73128734</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/humrep/deg162</oup_id><sourcerecordid>345544921</sourcerecordid><originalsourceid>FETCH-LOGICAL-c522t-3590981fc99914bd1dfa1c90d50f1adf3c04116774e1b8464e1ba849d6d90fa03</originalsourceid><addsrcrecordid>eNqF0M1O3DAUBWALUcFAu2SLIiSqblJ87cSxl2hEO1WnKpVAqthYHv9AIImDPYFOV32EPmOfpIkSgcSG1bWsT_ceHYQOAH8ELOjJTVcH254Yew2MbKEZZAynhOZ4G80wYTwFYLCL9mK8xbh_craDdoEwhklBZmh-Wa2D-vfnb1BtaRIXrP1dNteJd8mDDZuk8o9J09UrG-LwF1sb6qSLA9Fh4yvv2_gWvXGqivbdNPfR5aezi_kiXX7__GV-ukx1Tsg6pbnAgoPTQgjIVgaMU6AFNjl2oIyjGmd92KLILKx4xoaheCYMMwI7hek-ej_ubYO_72xcy7qM2laVaqzvoiwoEF7QrIdHL-Ct70LTZ5MEgBdcCN6jdEQ6-BiDdbINZa3CRgKWQ7VyrFaO1fb-cFrarWprnvXUZQ-OJ6CiVpULqtFlfHaZAEH5cPjD6HzXvnpzyljGtf31hFW4k6ygRS4XP6_kxZL9OP92tZRf6X_jbqDV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>211878998</pqid></control><display><type>article</type><title>Ultra‐rapid freezing of very low numbers of sperm using cryoloops</title><source>MEDLINE</source><source>Oxford University Press Journals Current</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Schuster, Timothy G. ; Keller, Laura M. ; Dunn, Rodney L. ; Ohl, Dana A. ; Smith, Gary D.</creator><creatorcontrib>Schuster, Timothy G. ; Keller, Laura M. ; Dunn, Rodney L. ; Ohl, Dana A. ; Smith, Gary D.</creatorcontrib><description>BACKGROUND: With the availability of ICSI, men with severe oligozoospermia (<5×106/ml) are able to reproduce. Current methods for cryopreservation of severe oligozoospermic samples are labour intensive and costly. The objective of this study was to evaluate whether freezing small numbers of motile sperm (∼100) was feasible using cryoloops. METHODS: Initial tests assessed the effect of various dilutions of cryoprotectants on pre‐freezing sperm motility. Several solutions were further evaluated for their ability to cryoprotect sperm during ultra‐rapid freezing. Sperm were placed on cryoloops and held in liquid nitrogen vapour for 5 min prior to freezing (ultra‐rapid freezing) or directly submerged into liquid nitrogen. Using the optimal cryoprotectant and technique from these experiments, ultra‐rapid and standard slow‐rate freezing protocols were compared. RESULTS: Optimal sperm survival was seen when sperm in cryoloops were placed in liquid nitrogen vapour in test yolk buffer with 12% v/v glycerol versus other cryoprotectants. Using this cryoprotectant, post‐thaw sperm motility is comparable between ultra‐rapid and slow‐rate freezing methods. CONCLUSION: Ultra‐rapid freezing of very low numbers of sperm is feasible using cryoloops suspended in liquid nitrogen vapour for 5 min.</description><identifier>ISSN: 0268-1161</identifier><identifier>ISSN: 1460-2350</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/deg162</identifier><identifier>PMID: 12660272</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Cell Survival ; Cryopreservation - instrumentation ; Cryoprotective Agents - pharmacology ; Feasibility Studies ; Fundamental and applied biological sciences. Psychology ; Glycerol - pharmacology ; Humans ; Key words: cryopreservation/oligozoospermia/sperm/ultra‐rapid freezing ; Male ; Mammalian male genital system ; Morphology. Physiology ; Sperm Motility - drug effects ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Time Factors ; Vertebrates: reproduction</subject><ispartof>Human reproduction (Oxford), 2003-04, Vol.18 (4), p.788-795</ispartof><rights>2003</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Apr 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-3590981fc99914bd1dfa1c90d50f1adf3c04116774e1b8464e1ba849d6d90fa03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14919388$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12660272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schuster, Timothy G.</creatorcontrib><creatorcontrib>Keller, Laura M.</creatorcontrib><creatorcontrib>Dunn, Rodney L.</creatorcontrib><creatorcontrib>Ohl, Dana A.</creatorcontrib><creatorcontrib>Smith, Gary D.</creatorcontrib><title>Ultra‐rapid freezing of very low numbers of sperm using cryoloops</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>BACKGROUND: With the availability of ICSI, men with severe oligozoospermia (<5×106/ml) are able to reproduce. Current methods for cryopreservation of severe oligozoospermic samples are labour intensive and costly. The objective of this study was to evaluate whether freezing small numbers of motile sperm (∼100) was feasible using cryoloops. METHODS: Initial tests assessed the effect of various dilutions of cryoprotectants on pre‐freezing sperm motility. Several solutions were further evaluated for their ability to cryoprotect sperm during ultra‐rapid freezing. Sperm were placed on cryoloops and held in liquid nitrogen vapour for 5 min prior to freezing (ultra‐rapid freezing) or directly submerged into liquid nitrogen. Using the optimal cryoprotectant and technique from these experiments, ultra‐rapid and standard slow‐rate freezing protocols were compared. RESULTS: Optimal sperm survival was seen when sperm in cryoloops were placed in liquid nitrogen vapour in test yolk buffer with 12% v/v glycerol versus other cryoprotectants. Using this cryoprotectant, post‐thaw sperm motility is comparable between ultra‐rapid and slow‐rate freezing methods. CONCLUSION: Ultra‐rapid freezing of very low numbers of sperm is feasible using cryoloops suspended in liquid nitrogen vapour for 5 min.</description><subject>Biological and medical sciences</subject><subject>Cell Survival</subject><subject>Cryopreservation - instrumentation</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Feasibility Studies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycerol - pharmacology</subject><subject>Humans</subject><subject>Key words: cryopreservation/oligozoospermia/sperm/ultra‐rapid freezing</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Time Factors</subject><subject>Vertebrates: reproduction</subject><issn>0268-1161</issn><issn>1460-2350</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M1O3DAUBWALUcFAu2SLIiSqblJ87cSxl2hEO1WnKpVAqthYHv9AIImDPYFOV32EPmOfpIkSgcSG1bWsT_ceHYQOAH8ELOjJTVcH254Yew2MbKEZZAynhOZ4G80wYTwFYLCL9mK8xbh_craDdoEwhklBZmh-Wa2D-vfnb1BtaRIXrP1dNteJd8mDDZuk8o9J09UrG-LwF1sb6qSLA9Fh4yvv2_gWvXGqivbdNPfR5aezi_kiXX7__GV-ukx1Tsg6pbnAgoPTQgjIVgaMU6AFNjl2oIyjGmd92KLILKx4xoaheCYMMwI7hek-ej_ubYO_72xcy7qM2laVaqzvoiwoEF7QrIdHL-Ct70LTZ5MEgBdcCN6jdEQ6-BiDdbINZa3CRgKWQ7VyrFaO1fb-cFrarWprnvXUZQ-OJ6CiVpULqtFlfHaZAEH5cPjD6HzXvnpzyljGtf31hFW4k6ygRS4XP6_kxZL9OP92tZRf6X_jbqDV</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Schuster, Timothy G.</creator><creator>Keller, Laura M.</creator><creator>Dunn, Rodney L.</creator><creator>Ohl, Dana A.</creator><creator>Smith, Gary D.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Ultra‐rapid freezing of very low numbers of sperm using cryoloops</title><author>Schuster, Timothy G. ; Keller, Laura M. ; Dunn, Rodney L. ; Ohl, Dana A. ; Smith, Gary D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-3590981fc99914bd1dfa1c90d50f1adf3c04116774e1b8464e1ba849d6d90fa03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Cell Survival</topic><topic>Cryopreservation - instrumentation</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Feasibility Studies</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycerol - pharmacology</topic><topic>Humans</topic><topic>Key words: cryopreservation/oligozoospermia/sperm/ultra‐rapid freezing</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Time Factors</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schuster, Timothy G.</creatorcontrib><creatorcontrib>Keller, Laura M.</creatorcontrib><creatorcontrib>Dunn, Rodney L.</creatorcontrib><creatorcontrib>Ohl, Dana A.</creatorcontrib><creatorcontrib>Smith, Gary D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schuster, Timothy G.</au><au>Keller, Laura M.</au><au>Dunn, Rodney L.</au><au>Ohl, Dana A.</au><au>Smith, Gary D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultra‐rapid freezing of very low numbers of sperm using cryoloops</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>18</volume><issue>4</issue><spage>788</spage><epage>795</epage><pages>788-795</pages><issn>0268-1161</issn><issn>1460-2350</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND: With the availability of ICSI, men with severe oligozoospermia (<5×106/ml) are able to reproduce. Current methods for cryopreservation of severe oligozoospermic samples are labour intensive and costly. The objective of this study was to evaluate whether freezing small numbers of motile sperm (∼100) was feasible using cryoloops. METHODS: Initial tests assessed the effect of various dilutions of cryoprotectants on pre‐freezing sperm motility. Several solutions were further evaluated for their ability to cryoprotect sperm during ultra‐rapid freezing. Sperm were placed on cryoloops and held in liquid nitrogen vapour for 5 min prior to freezing (ultra‐rapid freezing) or directly submerged into liquid nitrogen. Using the optimal cryoprotectant and technique from these experiments, ultra‐rapid and standard slow‐rate freezing protocols were compared. RESULTS: Optimal sperm survival was seen when sperm in cryoloops were placed in liquid nitrogen vapour in test yolk buffer with 12% v/v glycerol versus other cryoprotectants. Using this cryoprotectant, post‐thaw sperm motility is comparable between ultra‐rapid and slow‐rate freezing methods. CONCLUSION: Ultra‐rapid freezing of very low numbers of sperm is feasible using cryoloops suspended in liquid nitrogen vapour for 5 min.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>12660272</pmid><doi>10.1093/humrep/deg162</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-1161 |
| ispartof | Human reproduction (Oxford), 2003-04, Vol.18 (4), p.788-795 |
| issn | 0268-1161 1460-2350 1460-2350 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73128734 |
| source | MEDLINE; Oxford University Press Journals Current; EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Cell Survival Cryopreservation - instrumentation Cryoprotective Agents - pharmacology Feasibility Studies Fundamental and applied biological sciences. Psychology Glycerol - pharmacology Humans Key words: cryopreservation/oligozoospermia/sperm/ultra‐rapid freezing Male Mammalian male genital system Morphology. Physiology Sperm Motility - drug effects Spermatozoa - drug effects Spermatozoa - physiology Time Factors Vertebrates: reproduction |
| title | Ultra‐rapid freezing of very low numbers of sperm using cryoloops |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T04%3A59%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ultra%E2%80%90rapid%20freezing%20of%20very%20low%20numbers%20of%20sperm%20using%20cryoloops&rft.jtitle=Human%20reproduction%20(Oxford)&rft.au=Schuster,%20Timothy%20G.&rft.date=2003-04-01&rft.volume=18&rft.issue=4&rft.spage=788&rft.epage=795&rft.pages=788-795&rft.issn=0268-1161&rft.eissn=1460-2350&rft.coden=HUREEE&rft_id=info:doi/10.1093/humrep/deg162&rft_dat=%3Cproquest_cross%3E345544921%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=211878998&rft_id=info:pmid/12660272&rft_oup_id=10.1093/humrep/deg162&rfr_iscdi=true |