Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces
1 Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan 2 Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan 3 Researc...
Gespeichert in:
| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2003-01, Vol.53 (1), p.263-268 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 268 |
|---|---|
| container_issue | 1 |
| container_start_page | 263 |
| container_title | International journal of systematic and evolutionary microbiology |
| container_volume | 53 |
| creator | Niamsup, Piyanuch Sujaya, I Nengah Tanaka, Michiko Sone, Teruo Hanada, Satoshi Kamagata, Yoichi Lumyong, Saisamorn Assavanig, Apinya Asano, Kozo Tomita, Fusao Yokota, Atsushi |
| description | 1 Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan
2 Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan
3 Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan
4 Department of Biology, Faculty of Science, Chiang Mai University, Huay Kaew Road, Muang District, Chiang Mai 50200, Thailand
5 Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Correspondence Atsushi Yokota yokota{at}chem.agr.hokudai.ac.jp
Five strains of thermotolerant lactic acid bacteria (G 12, G 22, G 35 T , G 43 and G 44) isolated from chicken faeces were characterized taxonomically. The strains were facultatively anaerobic, Gram-positive, catalase-negative, non-motile, non-spore-forming rods. They were heterofermentative lactobacilli that produced DL -lactic acid. Growth of the strains occurred at 45 °C but not at 15 °C. The optimum temperature for growth was 42 °C, as determined from the specific growth rate. The highest permissive temperatures for growth were 50 °C for strain G 35 T and 48 °C for the other four strains. DNA G+C content of the strains was between 49 and 51 mol%. Complex fatty acid patterns of the strains showed the presence of C 14 : 0 , C 16 : 0 , sometimes C 18 : 0 , C 18 : 1 and C 19 : 0 cyclo in the cell walls. Phylogenetic analysis of the 16S rRNA gene sequences of the five strains placed them in the Lactobacillus casei / Pediococcus group, with Lactobacillus fermentum as their closest relative (about 95 % sequence similarity). DNADNA hybridization data indicated that the thermotolerant strains were not L. fermentum . Taken together, the findings of this study show that the five strains isolated from chicken faeces represent a novel species within the genus Lactobacillus , for which the name Lactobacillus thermotolerans is proposed (G 35 T =DSM 14792 T =JCM 11425 T ).
Abbreviations: LAB, lactic acid bacteria; TLAB, thermotolerant lactic acid bacteria
Published online ahead of print on 19 July 2002 as DOI 10.1099/ijs.0.02347-0.
The GenBank accession numbers for the 16S rDNA sequences of strains G 12, G 22, G 35 T , G 43 and G 44 are AF308146 , AF308147 , AF317702 , AF317703 and AF317704 , respectively. |
| doi_str_mv | 10.1099/ijs.0.02347-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73127867</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18760885</sourcerecordid><originalsourceid>FETCH-LOGICAL-c465t-e7875f545427b992b21a1aaee5fc65216ef911609d700ecff6ffe72c5299ebef3</originalsourceid><addsrcrecordid>eNqF0MFu1DAQBmALgWgpHHtFuQCXJoyd2E6OVQUFaSUu9Gw53nHXrRMvnmwRb4-3u6LqidOMRp9mRj9j5xwaDsPwOdxRAw2IttM1vGCnvFOqltB2L__1Qp2wN0R3AGUA8JqdcKGk4n17ysaVdUsarQsx7qhaNpintKSI2c5U0bap5vTQXFR2XzE-B0sB6AJSFShFu-C68jlNldsEd49z5S06pLfslbeR8N2xnrGbr19-Xn2rVz-uv19drmrXKbnUqHstvexkJ_Q4DGIU3HJrEaV3Sgqu0A-cKxjWGgCd98p71MJJMQw4om_P2MfD3m1Ov3ZIi5kCOYzRzph2ZHTLhe6V_i_kvVbQ97LA-gBdTkQZvdnmMNn8x3Aw-_RNSd-AeUzfQPHvj4t344TrJ32Mu4APR2DJ2ehLhi7Qk-ukKHe74j4d3Cbcbn6HjOYW5ymUN8aQ9kdla7gRqm3_AvcXnIM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18760885</pqid></control><display><type>article</type><title>Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces</title><source>MEDLINE</source><source>Microbiology Society</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Niamsup, Piyanuch ; Sujaya, I Nengah ; Tanaka, Michiko ; Sone, Teruo ; Hanada, Satoshi ; Kamagata, Yoichi ; Lumyong, Saisamorn ; Assavanig, Apinya ; Asano, Kozo ; Tomita, Fusao ; Yokota, Atsushi</creator><creatorcontrib>Niamsup, Piyanuch ; Sujaya, I Nengah ; Tanaka, Michiko ; Sone, Teruo ; Hanada, Satoshi ; Kamagata, Yoichi ; Lumyong, Saisamorn ; Assavanig, Apinya ; Asano, Kozo ; Tomita, Fusao ; Yokota, Atsushi</creatorcontrib><description>1 Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan
2 Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan
3 Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan
4 Department of Biology, Faculty of Science, Chiang Mai University, Huay Kaew Road, Muang District, Chiang Mai 50200, Thailand
5 Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Correspondence Atsushi Yokota yokota{at}chem.agr.hokudai.ac.jp
Five strains of thermotolerant lactic acid bacteria (G 12, G 22, G 35 T , G 43 and G 44) isolated from chicken faeces were characterized taxonomically. The strains were facultatively anaerobic, Gram-positive, catalase-negative, non-motile, non-spore-forming rods. They were heterofermentative lactobacilli that produced DL -lactic acid. Growth of the strains occurred at 45 °C but not at 15 °C. The optimum temperature for growth was 42 °C, as determined from the specific growth rate. The highest permissive temperatures for growth were 50 °C for strain G 35 T and 48 °C for the other four strains. DNA G+C content of the strains was between 49 and 51 mol%. Complex fatty acid patterns of the strains showed the presence of C 14 : 0 , C 16 : 0 , sometimes C 18 : 0 , C 18 : 1 and C 19 : 0 cyclo in the cell walls. Phylogenetic analysis of the 16S rRNA gene sequences of the five strains placed them in the Lactobacillus casei / Pediococcus group, with Lactobacillus fermentum as their closest relative (about 95 % sequence similarity). DNADNA hybridization data indicated that the thermotolerant strains were not L. fermentum . Taken together, the findings of this study show that the five strains isolated from chicken faeces represent a novel species within the genus Lactobacillus , for which the name Lactobacillus thermotolerans is proposed (G 35 T =DSM 14792 T =JCM 11425 T ).
Abbreviations: LAB, lactic acid bacteria; TLAB, thermotolerant lactic acid bacteria
Published online ahead of print on 19 July 2002 as DOI 10.1099/ijs.0.02347-0.
The GenBank accession numbers for the 16S rDNA sequences of strains G 12, G 22, G 35 T , G 43 and G 44 are AF308146 , AF308147 , AF317702 , AF317703 and AF317704 , respectively.
A fuller phylogenetic tree showing the placement of Lactobacillus thermotolerans within the genus Lactobacillus (Fig. I) and a growth curve for strain G 35 T (Fig. II) are available as supplementary data in IJSEM Online ( http://ijs.sgmjournals.org ).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.02347-0</identifier><identifier>PMID: 12656183</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Bacteriology ; Base Composition ; Biological and medical sciences ; Chickens - microbiology ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Fatty Acids - analysis ; Feces - microbiology ; Fundamental and applied biological sciences. Psychology ; Hot Temperature ; Lactobacillus - classification ; Lactobacillus - genetics ; Lactobacillus - isolation & purification ; Lactobacillus - metabolism ; Microbiology ; Molecular Sequence Data ; Phenotype ; Phylogeny ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; Systematics</subject><ispartof>International journal of systematic and evolutionary microbiology, 2003-01, Vol.53 (1), p.263-268</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-e7875f545427b992b21a1aaee5fc65216ef911609d700ecff6ffe72c5299ebef3</citedby><cites>FETCH-LOGICAL-c465t-e7875f545427b992b21a1aaee5fc65216ef911609d700ecff6ffe72c5299ebef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14528854$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12656183$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niamsup, Piyanuch</creatorcontrib><creatorcontrib>Sujaya, I Nengah</creatorcontrib><creatorcontrib>Tanaka, Michiko</creatorcontrib><creatorcontrib>Sone, Teruo</creatorcontrib><creatorcontrib>Hanada, Satoshi</creatorcontrib><creatorcontrib>Kamagata, Yoichi</creatorcontrib><creatorcontrib>Lumyong, Saisamorn</creatorcontrib><creatorcontrib>Assavanig, Apinya</creatorcontrib><creatorcontrib>Asano, Kozo</creatorcontrib><creatorcontrib>Tomita, Fusao</creatorcontrib><creatorcontrib>Yokota, Atsushi</creatorcontrib><title>Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>1 Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan
2 Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan
3 Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan
4 Department of Biology, Faculty of Science, Chiang Mai University, Huay Kaew Road, Muang District, Chiang Mai 50200, Thailand
5 Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Correspondence Atsushi Yokota yokota{at}chem.agr.hokudai.ac.jp
Five strains of thermotolerant lactic acid bacteria (G 12, G 22, G 35 T , G 43 and G 44) isolated from chicken faeces were characterized taxonomically. The strains were facultatively anaerobic, Gram-positive, catalase-negative, non-motile, non-spore-forming rods. They were heterofermentative lactobacilli that produced DL -lactic acid. Growth of the strains occurred at 45 °C but not at 15 °C. The optimum temperature for growth was 42 °C, as determined from the specific growth rate. The highest permissive temperatures for growth were 50 °C for strain G 35 T and 48 °C for the other four strains. DNA G+C content of the strains was between 49 and 51 mol%. Complex fatty acid patterns of the strains showed the presence of C 14 : 0 , C 16 : 0 , sometimes C 18 : 0 , C 18 : 1 and C 19 : 0 cyclo in the cell walls. Phylogenetic analysis of the 16S rRNA gene sequences of the five strains placed them in the Lactobacillus casei / Pediococcus group, with Lactobacillus fermentum as their closest relative (about 95 % sequence similarity). DNADNA hybridization data indicated that the thermotolerant strains were not L. fermentum . Taken together, the findings of this study show that the five strains isolated from chicken faeces represent a novel species within the genus Lactobacillus , for which the name Lactobacillus thermotolerans is proposed (G 35 T =DSM 14792 T =JCM 11425 T ).
Abbreviations: LAB, lactic acid bacteria; TLAB, thermotolerant lactic acid bacteria
Published online ahead of print on 19 July 2002 as DOI 10.1099/ijs.0.02347-0.
The GenBank accession numbers for the 16S rDNA sequences of strains G 12, G 22, G 35 T , G 43 and G 44 are AF308146 , AF308147 , AF317702 , AF317703 and AF317704 , respectively.
A fuller phylogenetic tree showing the placement of Lactobacillus thermotolerans within the genus Lactobacillus (Fig. I) and a growth curve for strain G 35 T (Fig. II) are available as supplementary data in IJSEM Online ( http://ijs.sgmjournals.org ).</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Base Composition</subject><subject>Biological and medical sciences</subject><subject>Chickens - microbiology</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fatty Acids - analysis</subject><subject>Feces - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hot Temperature</subject><subject>Lactobacillus - classification</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus - isolation & purification</subject><subject>Lactobacillus - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Systematics</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MFu1DAQBmALgWgpHHtFuQCXJoyd2E6OVQUFaSUu9Gw53nHXrRMvnmwRb4-3u6LqidOMRp9mRj9j5xwaDsPwOdxRAw2IttM1vGCnvFOqltB2L__1Qp2wN0R3AGUA8JqdcKGk4n17ysaVdUsarQsx7qhaNpintKSI2c5U0bap5vTQXFR2XzE-B0sB6AJSFShFu-C68jlNldsEd49z5S06pLfslbeR8N2xnrGbr19-Xn2rVz-uv19drmrXKbnUqHstvexkJ_Q4DGIU3HJrEaV3Sgqu0A-cKxjWGgCd98p71MJJMQw4om_P2MfD3m1Ov3ZIi5kCOYzRzph2ZHTLhe6V_i_kvVbQ97LA-gBdTkQZvdnmMNn8x3Aw-_RNSd-AeUzfQPHvj4t344TrJ32Mu4APR2DJ2ehLhi7Qk-ukKHe74j4d3Cbcbn6HjOYW5ymUN8aQ9kdla7gRqm3_AvcXnIM</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Niamsup, Piyanuch</creator><creator>Sujaya, I Nengah</creator><creator>Tanaka, Michiko</creator><creator>Sone, Teruo</creator><creator>Hanada, Satoshi</creator><creator>Kamagata, Yoichi</creator><creator>Lumyong, Saisamorn</creator><creator>Assavanig, Apinya</creator><creator>Asano, Kozo</creator><creator>Tomita, Fusao</creator><creator>Yokota, Atsushi</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces</title><author>Niamsup, Piyanuch ; Sujaya, I Nengah ; Tanaka, Michiko ; Sone, Teruo ; Hanada, Satoshi ; Kamagata, Yoichi ; Lumyong, Saisamorn ; Assavanig, Apinya ; Asano, Kozo ; Tomita, Fusao ; Yokota, Atsushi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-e7875f545427b992b21a1aaee5fc65216ef911609d700ecff6ffe72c5299ebef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Bacteriology</topic><topic>Base Composition</topic><topic>Biological and medical sciences</topic><topic>Chickens - microbiology</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fatty Acids - analysis</topic><topic>Feces - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hot Temperature</topic><topic>Lactobacillus - classification</topic><topic>Lactobacillus - genetics</topic><topic>Lactobacillus - isolation & purification</topic><topic>Lactobacillus - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Systematics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Niamsup, Piyanuch</creatorcontrib><creatorcontrib>Sujaya, I Nengah</creatorcontrib><creatorcontrib>Tanaka, Michiko</creatorcontrib><creatorcontrib>Sone, Teruo</creatorcontrib><creatorcontrib>Hanada, Satoshi</creatorcontrib><creatorcontrib>Kamagata, Yoichi</creatorcontrib><creatorcontrib>Lumyong, Saisamorn</creatorcontrib><creatorcontrib>Assavanig, Apinya</creatorcontrib><creatorcontrib>Asano, Kozo</creatorcontrib><creatorcontrib>Tomita, Fusao</creatorcontrib><creatorcontrib>Yokota, Atsushi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niamsup, Piyanuch</au><au>Sujaya, I Nengah</au><au>Tanaka, Michiko</au><au>Sone, Teruo</au><au>Hanada, Satoshi</au><au>Kamagata, Yoichi</au><au>Lumyong, Saisamorn</au><au>Assavanig, Apinya</au><au>Asano, Kozo</au><au>Tomita, Fusao</au><au>Yokota, Atsushi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>53</volume><issue>1</issue><spage>263</spage><epage>268</epage><pages>263-268</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>1 Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan
2 Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan
3 Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan
4 Department of Biology, Faculty of Science, Chiang Mai University, Huay Kaew Road, Muang District, Chiang Mai 50200, Thailand
5 Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Correspondence Atsushi Yokota yokota{at}chem.agr.hokudai.ac.jp
Five strains of thermotolerant lactic acid bacteria (G 12, G 22, G 35 T , G 43 and G 44) isolated from chicken faeces were characterized taxonomically. The strains were facultatively anaerobic, Gram-positive, catalase-negative, non-motile, non-spore-forming rods. They were heterofermentative lactobacilli that produced DL -lactic acid. Growth of the strains occurred at 45 °C but not at 15 °C. The optimum temperature for growth was 42 °C, as determined from the specific growth rate. The highest permissive temperatures for growth were 50 °C for strain G 35 T and 48 °C for the other four strains. DNA G+C content of the strains was between 49 and 51 mol%. Complex fatty acid patterns of the strains showed the presence of C 14 : 0 , C 16 : 0 , sometimes C 18 : 0 , C 18 : 1 and C 19 : 0 cyclo in the cell walls. Phylogenetic analysis of the 16S rRNA gene sequences of the five strains placed them in the Lactobacillus casei / Pediococcus group, with Lactobacillus fermentum as their closest relative (about 95 % sequence similarity). DNADNA hybridization data indicated that the thermotolerant strains were not L. fermentum . Taken together, the findings of this study show that the five strains isolated from chicken faeces represent a novel species within the genus Lactobacillus , for which the name Lactobacillus thermotolerans is proposed (G 35 T =DSM 14792 T =JCM 11425 T ).
Abbreviations: LAB, lactic acid bacteria; TLAB, thermotolerant lactic acid bacteria
Published online ahead of print on 19 July 2002 as DOI 10.1099/ijs.0.02347-0.
The GenBank accession numbers for the 16S rDNA sequences of strains G 12, G 22, G 35 T , G 43 and G 44 are AF308146 , AF308147 , AF317702 , AF317703 and AF317704 , respectively.
A fuller phylogenetic tree showing the placement of Lactobacillus thermotolerans within the genus Lactobacillus (Fig. I) and a growth curve for strain G 35 T (Fig. II) are available as supplementary data in IJSEM Online ( http://ijs.sgmjournals.org ).</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>12656183</pmid><doi>10.1099/ijs.0.02347-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2003-01, Vol.53 (1), p.263-268 |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73127867 |
| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Bacteriology Base Composition Biological and medical sciences Chickens - microbiology DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - genetics Fatty Acids - analysis Feces - microbiology Fundamental and applied biological sciences. Psychology Hot Temperature Lactobacillus - classification Lactobacillus - genetics Lactobacillus - isolation & purification Lactobacillus - metabolism Microbiology Molecular Sequence Data Phenotype Phylogeny RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Systematics |
| title | Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T01%3A37%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lactobacillus%20thermotolerans%20sp.%20nov.,%20a%20novel%20thermotolerant%20species%20isolated%20from%20chicken%20faeces&rft.jtitle=International%20journal%20of%20systematic%20and%20evolutionary%20microbiology&rft.au=Niamsup,%20Piyanuch&rft.date=2003-01-01&rft.volume=53&rft.issue=1&rft.spage=263&rft.epage=268&rft.pages=263-268&rft.issn=1466-5026&rft.eissn=1466-5034&rft_id=info:doi/10.1099/ijs.0.02347-0&rft_dat=%3Cproquest_cross%3E18760885%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18760885&rft_id=info:pmid/12656183&rfr_iscdi=true |