Physical–chemical features of non-detergent sulfobetaines active as protein-folding helpers
Some non-detergent sulfobetaines had been shown to prevent aggregation and improve the yield of active proteins when added to the buffer during in vitro protein renaturation. With the aim of designing more efficient folding helpers, a series of non-detergent sulfobetaines have been synthesized and t...
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| Veröffentlicht in: | Biophysical chemistry 2002-12, Vol.100 (1-3), p.469-479 |
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| container_title | Biophysical chemistry |
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| creator | Expert-Bezançon, Nicole Rabilloud, Thierry Vuillard, Laurent Goldberg, Michel E |
| description | Some non-detergent sulfobetaines had been shown to prevent aggregation and improve the yield of active proteins when added to the buffer during in vitro protein renaturation. With the aim of designing more efficient folding helpers, a series of non-detergent sulfobetaines have been synthesized and their efficiency in improving the renaturation of a variety of proteins (E. coli tryptophan synthase and β-d-galactosidase, hen lysozyme, bovine serum albumin, a monoclonal antibody) have been investigated. Attempts to correlate the structure of each sulfobetaines with its effect on folding revealed some molecular features that appear important in helping renaturation. This enabled us to design and synthesize new non-detergent sulfobetaines that act as potent folding helpers. |
| doi_str_mv | 10.1016/S0301-4622(02)00299-5 |
| format | Article |
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| identifier | ISSN: 0301-4622 |
| ispartof | Biophysical chemistry, 2002-12, Vol.100 (1-3), p.469-479 |
| issn | 0301-4622 1873-4200 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Blocking - chemistry Antibodies, Monoclonal - chemistry Betaine - analogs & derivatives Betaine - chemistry Cattle Chemical Phenomena Chemistry, Physical Chickens Drug Design Mice Muramidase - chemistry Non-detergent sulfobetaines Physical–chemical features Protein Folding Protein Renaturation Protein-folding helpers Proteins - chemistry Serum Albumin, Bovine - chemistry Tryptophan Synthase - antagonists & inhibitors Tryptophan Synthase - chemistry |
| title | Physical–chemical features of non-detergent sulfobetaines active as protein-folding helpers |
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