Regulation of ipsilateral and contralateral bovine oviduct epithelial cell function in the postovulation period: a transcriptomics approach
We studied differential gene expression in ipsilateral and contralateral bovine oviduct epithelial cells using a combination of subtracted cDNA libraries and cDNA array hybridization. Four Simmental heifers were synchronized and slaughtered 3.5 days after they entered standing heat. Epithelial cells...
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| Veröffentlicht in: | Biology of reproduction 2003-04, Vol.68 (4), p.1170-1177 |
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| creator | Bauersachs, S Blum, H Mallok, S Wenigerkind, H Rief, S Prelle, K Wolf, E |
| description | We studied differential gene expression in ipsilateral and contralateral bovine oviduct epithelial cells using a combination
of subtracted cDNA libraries and cDNA array hybridization. Four Simmental heifers were synchronized and slaughtered 3.5 days
after they entered standing heat. Epithelial cells were isolated from ipsilateral and contralateral oviducts. To identify
genes that are differentially regulated in ipsilateral and contralateral epithelium, subtracted cDNA libraries were produced
by suppression subtractive hybridization and analyzed by cDNA array hybridization. Sequencing of cDNAs showing differential
expression levels in ipsilateral and contralateral epithelium revealed 35 different cDNAs, 30 of which matched genes with
known functions and 5 of which matched genes without a known function. The majority of genes (n = 27) were expressed at a
higher level in the ipsilateral oviduct, but for some genes (n = 8), mRNA abundance was higher in the contralateral oviduct.
The regulated genes or their products include a variety of functional classes such as cell-surface proteins, cell-cell interaction
proteins, members of signal transduction pathways, immune-related proteins, and enzymes. Identification of genes differentially
regulated in ipsilateral and contralateral oviduct epithelial cells is the first step toward a systematic analysis of local
mechanisms that regulate the function of the bovine oviduct epithelium in the postovulation period. |
| doi_str_mv | 10.1095/biolreprod.102.010660 |
| format | Article |
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of subtracted cDNA libraries and cDNA array hybridization. Four Simmental heifers were synchronized and slaughtered 3.5 days
after they entered standing heat. Epithelial cells were isolated from ipsilateral and contralateral oviducts. To identify
genes that are differentially regulated in ipsilateral and contralateral epithelium, subtracted cDNA libraries were produced
by suppression subtractive hybridization and analyzed by cDNA array hybridization. Sequencing of cDNAs showing differential
expression levels in ipsilateral and contralateral epithelium revealed 35 different cDNAs, 30 of which matched genes with
known functions and 5 of which matched genes without a known function. The majority of genes (n = 27) were expressed at a
higher level in the ipsilateral oviduct, but for some genes (n = 8), mRNA abundance was higher in the contralateral oviduct.
The regulated genes or their products include a variety of functional classes such as cell-surface proteins, cell-cell interaction
proteins, members of signal transduction pathways, immune-related proteins, and enzymes. Identification of genes differentially
regulated in ipsilateral and contralateral oviduct epithelial cells is the first step toward a systematic analysis of local
mechanisms that regulate the function of the bovine oviduct epithelium in the postovulation period.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.102.010660</identifier><identifier>PMID: 12606461</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; cDNA libraries ; differential gene expression ; epithelial cells ; Epithelial Cells - physiology ; estrous cycle ; Fallopian Tubes - cytology ; Fallopian Tubes - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Expression Profiling ; Gene Expression Regulation ; genes ; heifers ; Hormone metabolism and regulation ; Luteal Phase ; Mammalian female genital system ; messenger RNA ; microarray technology ; nucleic acid hybridization ; Oligonucleotide Array Sequence Analysis ; oviducts ; ovulation ; RNA, Messenger - metabolism ; spatial variation ; suppression subtractive hybridization ; Tissue Distribution ; transcription (genetics) ; Transcription, Genetic ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2003-04, Vol.68 (4), p.1170-1177</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15077199$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12606461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bauersachs, S</creatorcontrib><creatorcontrib>Blum, H</creatorcontrib><creatorcontrib>Mallok, S</creatorcontrib><creatorcontrib>Wenigerkind, H</creatorcontrib><creatorcontrib>Rief, S</creatorcontrib><creatorcontrib>Prelle, K</creatorcontrib><creatorcontrib>Wolf, E</creatorcontrib><title>Regulation of ipsilateral and contralateral bovine oviduct epithelial cell function in the postovulation period: a transcriptomics approach</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>We studied differential gene expression in ipsilateral and contralateral bovine oviduct epithelial cells using a combination
of subtracted cDNA libraries and cDNA array hybridization. Four Simmental heifers were synchronized and slaughtered 3.5 days
after they entered standing heat. Epithelial cells were isolated from ipsilateral and contralateral oviducts. To identify
genes that are differentially regulated in ipsilateral and contralateral epithelium, subtracted cDNA libraries were produced
by suppression subtractive hybridization and analyzed by cDNA array hybridization. Sequencing of cDNAs showing differential
expression levels in ipsilateral and contralateral epithelium revealed 35 different cDNAs, 30 of which matched genes with
known functions and 5 of which matched genes without a known function. The majority of genes (n = 27) were expressed at a
higher level in the ipsilateral oviduct, but for some genes (n = 8), mRNA abundance was higher in the contralateral oviduct.
The regulated genes or their products include a variety of functional classes such as cell-surface proteins, cell-cell interaction
proteins, members of signal transduction pathways, immune-related proteins, and enzymes. Identification of genes differentially
regulated in ipsilateral and contralateral oviduct epithelial cells is the first step toward a systematic analysis of local
mechanisms that regulate the function of the bovine oviduct epithelium in the postovulation period.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>cDNA libraries</subject><subject>differential gene expression</subject><subject>epithelial cells</subject><subject>Epithelial Cells - physiology</subject><subject>estrous cycle</subject><subject>Fallopian Tubes - cytology</subject><subject>Fallopian Tubes - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>genes</subject><subject>heifers</subject><subject>Hormone metabolism and regulation</subject><subject>Luteal Phase</subject><subject>Mammalian female genital system</subject><subject>messenger RNA</subject><subject>microarray technology</subject><subject>nucleic acid hybridization</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>oviducts</subject><subject>ovulation</subject><subject>RNA, Messenger - metabolism</subject><subject>spatial variation</subject><subject>suppression subtractive hybridization</subject><subject>Tissue Distribution</subject><subject>transcription (genetics)</subject><subject>Transcription, Genetic</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkd2O1SAQgInRuMfVR1C50buuA7RQvNts_Es2MVH3mkwpnIOhpUK7Jz6DLy26Z7M3DMN8-SYzEPKSwQUD3b0bQorZLTmNNecXwEBKeER2rOO6UVz2j8kOAGQjhBRn5FkpPwFYK7h4Ss4YlyBbyXbkzze33yKuIc00eRqWEmrmMkaK80htmtd6P70M6TbMjtZz3OxK3RLWg4uhVqyLkfpttv9FYaa1QJdU1nR7b19cDml8T5FW41xsDsuapmALxaWOgfbwnDzxGIt7cYrn5Objhx9Xn5vrr5--XF1eN55rvjacsx6GHkawEr2CVinsfK-89VppJrHlNUoAlDAM2FvQTMiRDy3TFnonzsnbO29t-2tzZTVTKP8mwNmlrRglGOed5hV8dQK3YXKjWXKYMP829-urwJsTgMVi9HUwG8oD14FSTOuHjoewPxxDdqZMGGPVCnM8HmVvWsOYggq-vgM9JoP7XGU33zkwAfXPte568RcFq5sZ</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Bauersachs, S</creator><creator>Blum, H</creator><creator>Mallok, S</creator><creator>Wenigerkind, H</creator><creator>Rief, S</creator><creator>Prelle, K</creator><creator>Wolf, E</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Regulation of ipsilateral and contralateral bovine oviduct epithelial cell function in the postovulation period: a transcriptomics approach</title><author>Bauersachs, S ; Blum, H ; Mallok, S ; Wenigerkind, H ; Rief, S ; Prelle, K ; Wolf, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f292t-22180b80d0c6af70477a5f87fcf97916a42979600a60bba8c09136d2b419c08e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>cDNA libraries</topic><topic>differential gene expression</topic><topic>epithelial cells</topic><topic>Epithelial Cells - physiology</topic><topic>estrous cycle</topic><topic>Fallopian Tubes - cytology</topic><topic>Fallopian Tubes - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>genes</topic><topic>heifers</topic><topic>Hormone metabolism and regulation</topic><topic>Luteal Phase</topic><topic>Mammalian female genital system</topic><topic>messenger RNA</topic><topic>microarray technology</topic><topic>nucleic acid hybridization</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>oviducts</topic><topic>ovulation</topic><topic>RNA, Messenger - metabolism</topic><topic>spatial variation</topic><topic>suppression subtractive hybridization</topic><topic>Tissue Distribution</topic><topic>transcription (genetics)</topic><topic>Transcription, Genetic</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bauersachs, S</creatorcontrib><creatorcontrib>Blum, H</creatorcontrib><creatorcontrib>Mallok, S</creatorcontrib><creatorcontrib>Wenigerkind, H</creatorcontrib><creatorcontrib>Rief, S</creatorcontrib><creatorcontrib>Prelle, K</creatorcontrib><creatorcontrib>Wolf, E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bauersachs, S</au><au>Blum, H</au><au>Mallok, S</au><au>Wenigerkind, H</au><au>Rief, S</au><au>Prelle, K</au><au>Wolf, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of ipsilateral and contralateral bovine oviduct epithelial cell function in the postovulation period: a transcriptomics approach</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>68</volume><issue>4</issue><spage>1170</spage><epage>1177</epage><pages>1170-1177</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>We studied differential gene expression in ipsilateral and contralateral bovine oviduct epithelial cells using a combination
of subtracted cDNA libraries and cDNA array hybridization. Four Simmental heifers were synchronized and slaughtered 3.5 days
after they entered standing heat. Epithelial cells were isolated from ipsilateral and contralateral oviducts. To identify
genes that are differentially regulated in ipsilateral and contralateral epithelium, subtracted cDNA libraries were produced
by suppression subtractive hybridization and analyzed by cDNA array hybridization. Sequencing of cDNAs showing differential
expression levels in ipsilateral and contralateral epithelium revealed 35 different cDNAs, 30 of which matched genes with
known functions and 5 of which matched genes without a known function. The majority of genes (n = 27) were expressed at a
higher level in the ipsilateral oviduct, but for some genes (n = 8), mRNA abundance was higher in the contralateral oviduct.
The regulated genes or their products include a variety of functional classes such as cell-surface proteins, cell-cell interaction
proteins, members of signal transduction pathways, immune-related proteins, and enzymes. Identification of genes differentially
regulated in ipsilateral and contralateral oviduct epithelial cells is the first step toward a systematic analysis of local
mechanisms that regulate the function of the bovine oviduct epithelium in the postovulation period.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12606461</pmid><doi>10.1095/biolreprod.102.010660</doi><tpages>8</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; BioOne Complete |
| subjects | Animals Biological and medical sciences Cattle cDNA libraries differential gene expression epithelial cells Epithelial Cells - physiology estrous cycle Fallopian Tubes - cytology Fallopian Tubes - physiology Female Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Profiling Gene Expression Regulation genes heifers Hormone metabolism and regulation Luteal Phase Mammalian female genital system messenger RNA microarray technology nucleic acid hybridization Oligonucleotide Array Sequence Analysis oviducts ovulation RNA, Messenger - metabolism spatial variation suppression subtractive hybridization Tissue Distribution transcription (genetics) Transcription, Genetic Vertebrates: reproduction |
| title | Regulation of ipsilateral and contralateral bovine oviduct epithelial cell function in the postovulation period: a transcriptomics approach |
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