Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins

Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the...

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Veröffentlicht in:	Glycobiology (Oxford) 2003-03, Vol.13 (3), p.169-177
Hauptverfasser:	Fontaine, Thierry, Magnin, Thierry, Melhert, Angela, Lamont, Douglas, Latgé, Jean-paul, Ferguson, Michael A.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	2-AB 2-aminobenzamide AHM anhydromannose Aspergillus Aspergillus fumigatus Aspergillus fumigatus - chemistry Blotting, Western bovine serum albumin BSA Carbohydrate Sequence Chromatography, High Pressure Liquid CRD cross-reacting determinant EDTA electrospray mass spectrometry electrospray tandem mass spectrometry ES-MS ES-MS-MS ethylenediamine tetra-acetic acid Fungal Proteins - chemistry glycoprotein glycosylphosphatidyl inositol glycosylphosphatidylinositol Glycosylphosphatidylinositols - chemistry GPI high-performance liquid chromatography HPLC inositol-phosphoceramide IPC MALDI-TOF Mass Spectrometry matrix-assisted laser desorption ionization time-of-flight Membrane Proteins - chemistry Molecular Sequence Data Peptides - chemistry phosphatidylinositol phospholipase C PLC polyvinylidene difluoride PVDF Saccharomyces cerevisiae SDS–PAGE sodium dodecyl sulfate–polyacrylamide gel electrophoresis
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