Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins
Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the...
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| Veröffentlicht in: | Glycobiology (Oxford) 2003-03, Vol.13 (3), p.169-177 |
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| creator | Fontaine, Thierry Magnin, Thierry Melhert, Angela Lamont, Douglas Latgé, Jean-paul Ferguson, Michael A.J. |
| description | Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. The A. fumigatus GPI glycan moiety is mainly a linear pentomannose structure linked to a glucosamine residue: Manα1-3Manα1-2Manα1-2Manα1-6Manα1-4GlcN. |
| doi_str_mv | 10.1093/glycob/cwg004 |
| format | Article |
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In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. 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In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. The A. fumigatus GPI glycan moiety is mainly a linear pentomannose structure linked to a glucosamine residue: Manα1-3Manα1-2Manα1-2Manα1-6Manα1-4GlcN.</description><subject>2-AB</subject><subject>2-aminobenzamide</subject><subject>AHM</subject><subject>anhydromannose</subject><subject>Aspergillus</subject><subject>Aspergillus fumigatus</subject><subject>Aspergillus fumigatus - chemistry</subject><subject>Blotting, Western</subject><subject>bovine serum albumin</subject><subject>BSA</subject><subject>Carbohydrate Sequence</subject><subject>Chromatography, High Pressure Liquid</subject><subject>CRD</subject><subject>cross-reacting determinant</subject><subject>EDTA</subject><subject>electrospray mass spectrometry</subject><subject>electrospray tandem mass spectrometry</subject><subject>ES-MS</subject><subject>ES-MS-MS</subject><subject>ethylenediamine tetra-acetic acid</subject><subject>Fungal Proteins - chemistry</subject><subject>glycoprotein</subject><subject>glycosylphosphatidyl inositol</subject><subject>glycosylphosphatidylinositol</subject><subject>Glycosylphosphatidylinositols - chemistry</subject><subject>GPI</subject><subject>high-performance liquid chromatography</subject><subject>HPLC</subject><subject>inositol-phosphoceramide</subject><subject>IPC</subject><subject>MALDI-TOF</subject><subject>Mass Spectrometry</subject><subject>matrix-assisted laser desorption ionization time-of-flight</subject><subject>Membrane Proteins - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Peptides - chemistry</subject><subject>phosphatidylinositol</subject><subject>phospholipase C</subject><subject>PLC</subject><subject>polyvinylidene difluoride</subject><subject>PVDF</subject><subject>Saccharomyces cerevisiae</subject><subject>SDS–PAGE</subject><subject>sodium dodecyl sulfate–polyacrylamide gel electrophoresis</subject><issn>0959-6658</issn><issn>1460-2423</issn><issn>1460-2423</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1rFDEYBvAgil2rR68yePA2Nm8-J8eyWCsUBFtBvIRMNrObmpmMSQbd_97UXVropaeE5MeTNzwIvQX8EbCiZ9uwt7E_s3-2GLNnaAVM4JYwQp-jFVZctULw7gS9yvkWYxDQ8ZfoBIgggmG2QuG6pMWWJbncxKEpO9f8T8z7MO9innem-M0--ClmX2JoRjf2yUyuMZPdxZSbIcWxOc-zS1sfwlIPltFvTam7ezunWJyf8mv0YjAhuzfH9RR9v_h0s75sr75-_rI-v2otU7i0PaPAOWZWEmIAOgJKGQlGWWEsk64zm54MfOOEkJg5Kyx3HeukFFzygVp6ij4ccuvDvxeXix59ti6EOkxcspYUCMFMPgkrYhxAVfj-EbyNS5rqJzQBTDHhnFbUHpBNMefkBj0nP5q014D1XVn6UJY-lFX9u2Po0o9u86CP7TwE-lzc3_t7k35pIank-vLHTy1BfLtW7EKv6T_dIqMM</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Fontaine, Thierry</creator><creator>Magnin, Thierry</creator><creator>Melhert, Angela</creator><creator>Lamont, Douglas</creator><creator>Latgé, Jean-paul</creator><creator>Ferguson, Michael A.J.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins</title><author>Fontaine, Thierry ; Magnin, Thierry ; Melhert, Angela ; Lamont, Douglas ; Latgé, Jean-paul ; Ferguson, Michael A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-b4315504c722a1182199a71a9c6ac47e8adb2f5de66704ec6c5e848776575f3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>2-AB</topic><topic>2-aminobenzamide</topic><topic>AHM</topic><topic>anhydromannose</topic><topic>Aspergillus</topic><topic>Aspergillus fumigatus</topic><topic>Aspergillus fumigatus - chemistry</topic><topic>Blotting, Western</topic><topic>bovine serum albumin</topic><topic>BSA</topic><topic>Carbohydrate Sequence</topic><topic>Chromatography, High Pressure Liquid</topic><topic>CRD</topic><topic>cross-reacting determinant</topic><topic>EDTA</topic><topic>electrospray mass spectrometry</topic><topic>electrospray tandem mass spectrometry</topic><topic>ES-MS</topic><topic>ES-MS-MS</topic><topic>ethylenediamine tetra-acetic acid</topic><topic>Fungal Proteins - chemistry</topic><topic>glycoprotein</topic><topic>glycosylphosphatidyl inositol</topic><topic>glycosylphosphatidylinositol</topic><topic>Glycosylphosphatidylinositols - chemistry</topic><topic>GPI</topic><topic>high-performance liquid chromatography</topic><topic>HPLC</topic><topic>inositol-phosphoceramide</topic><topic>IPC</topic><topic>MALDI-TOF</topic><topic>Mass Spectrometry</topic><topic>matrix-assisted laser desorption ionization time-of-flight</topic><topic>Membrane Proteins - chemistry</topic><topic>Molecular Sequence Data</topic><topic>Peptides - chemistry</topic><topic>phosphatidylinositol</topic><topic>phospholipase C</topic><topic>PLC</topic><topic>polyvinylidene difluoride</topic><topic>PVDF</topic><topic>Saccharomyces cerevisiae</topic><topic>SDS–PAGE</topic><topic>sodium dodecyl sulfate–polyacrylamide gel electrophoresis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fontaine, Thierry</creatorcontrib><creatorcontrib>Magnin, Thierry</creatorcontrib><creatorcontrib>Melhert, Angela</creatorcontrib><creatorcontrib>Lamont, Douglas</creatorcontrib><creatorcontrib>Latgé, Jean-paul</creatorcontrib><creatorcontrib>Ferguson, Michael A.J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Glycobiology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fontaine, Thierry</au><au>Magnin, Thierry</au><au>Melhert, Angela</au><au>Lamont, Douglas</au><au>Latgé, Jean-paul</au><au>Ferguson, Michael A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins</atitle><jtitle>Glycobiology (Oxford)</jtitle><addtitle>Glycobiology</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>13</volume><issue>3</issue><spage>169</spage><epage>177</epage><pages>169-177</pages><issn>0959-6658</issn><issn>1460-2423</issn><eissn>1460-2423</eissn><abstract>Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. The A. fumigatus GPI glycan moiety is mainly a linear pentomannose structure linked to a glucosamine residue: Manα1-3Manα1-2Manα1-2Manα1-6Manα1-4GlcN.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>12626404</pmid><doi>10.1093/glycob/cwg004</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 2-AB 2-aminobenzamide AHM anhydromannose Aspergillus Aspergillus fumigatus Aspergillus fumigatus - chemistry Blotting, Western bovine serum albumin BSA Carbohydrate Sequence Chromatography, High Pressure Liquid CRD cross-reacting determinant EDTA electrospray mass spectrometry electrospray tandem mass spectrometry ES-MS ES-MS-MS ethylenediamine tetra-acetic acid Fungal Proteins - chemistry glycoprotein glycosylphosphatidyl inositol glycosylphosphatidylinositol Glycosylphosphatidylinositols - chemistry GPI high-performance liquid chromatography HPLC inositol-phosphoceramide IPC MALDI-TOF Mass Spectrometry matrix-assisted laser desorption ionization time-of-flight Membrane Proteins - chemistry Molecular Sequence Data Peptides - chemistry phosphatidylinositol phospholipase C PLC polyvinylidene difluoride PVDF Saccharomyces cerevisiae SDS–PAGE sodium dodecyl sulfate–polyacrylamide gel electrophoresis |
| title | Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins |
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