Release of endogenous amino acids, including homocysteic acid and cysteine sulphinic acid, from rat hippocampal slices evoked by electrical stimulation of Schaffer collateral-commissural fibres
This study examined the release of endogenous amino acids from acute hippocampal slices, upon stimulation of the Schaffer collateral-commissural fibres. One-minute samples of superfusate were collected via a cannula placed over the CA1 stratum radiatum, and were analysed by reversed-phase high perfo...
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| Veröffentlicht in: | Neuroscience 1992, Vol.49 (3), p.557-570 |
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| creator | Klancnik, J.M. Cuénod, M. Gähwiler, B.H. Jiang, Z.P. Do, K.Q. |
| description | This study examined the release of endogenous amino acids from acute hippocampal slices, upon stimulation of the Schaffer collateral-commissural fibres. One-minute samples of superfusate were collected via a cannula placed over the CA1 stratum radiatum, and were analysed by reversed-phase high performance liquid chromatography. Evoked potentials were recorded to ascertain stimulation efficacy. Four minutes of continuous 50 Hz stimulation produced a tetrodotoxin-sensitive release of aspartate and glycine in the second minute of stimulation, as well as a tetrodotoxin-sensitive release of cysteine sulphinic acid, during stimulation and of homocysteic acid, following stimulation. Such 50 Hz stimulation also produced a tetrodotoxin-insensitive decrease in methionine levels, but no significant changes in any of the other 15 amino acids measured. Four minutes of continuous 1 Hz stimulation produced no changes in the levels of any of the amino acids measured, but four 600-ms trains of 100 Hz stimulation, which, unlike the 1 Hz stimulation, produced long-term potentiation, resulted in significant increases in levels of cysteine sulphinic acid and homocysteic acid, but not of any of the other amino acids measured.
These results suggest that aspartate, glycine, homocysteic acid, and cysteine sulphinic acid play a role in synaptic transmission in the Schaffer collateral-commissural fibres, and that cysteine sulphinic acid and homocysteic acid may be released specifically by high-frequency stimulation. |
| doi_str_mv | 10.1016/0306-4522(92)90226-R |
| format | Article |
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These results suggest that aspartate, glycine, homocysteic acid, and cysteine sulphinic acid play a role in synaptic transmission in the Schaffer collateral-commissural fibres, and that cysteine sulphinic acid and homocysteic acid may be released specifically by high-frequency stimulation.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/0306-4522(92)90226-R</identifier><identifier>PMID: 1354337</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>ACSF ; Amino Acids - metabolism ; analysis of variance ; Animals ; ANOVA ; artificial cerebrospinal fluid ; Aspartic Acid - metabolism ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; CSA ; Cysteine - analogs & derivatives ; Cysteine - metabolism ; cysteine sulphinic acid ; EAA ; Electric Stimulation ; Evoked Potentials ; excitatory amino acid ; Fundamental and applied biological sciences. Psychology ; gamma-Aminobutyric Acid - metabolism ; Glutamates - metabolism ; Glutamic Acid ; Glycine - metabolism ; HCA ; high-performance liquid chromatography ; Hippocampus - physiology ; homocysteic acid ; Homocysteine - analogs & derivatives ; Homocysteine - metabolism ; HPLC ; In Vitro Techniques ; Kinetics ; long-term potentiation ; LTP ; Male ; Methionine - metabolism ; N-methyl- d-aspartate ; Nerve Fibers - physiology ; Neurotransmitter Agents ; NMDA ; Pyramidal Tracts - physiology ; Rats ; Rats, Inbred Strains ; SAA ; sulphur-containing excitatory amino acids ; tetrodotoxin ; Time Factors ; TTX ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience, 1992, Vol.49 (3), p.557-570</ispartof><rights>1992 IBRO</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-ba49bcea4209857ac974200adb799a5f02405c6d9cc7c834ddbfc7d068aef4bc3</citedby><cites>FETCH-LOGICAL-c412t-ba49bcea4209857ac974200adb799a5f02405c6d9cc7c834ddbfc7d068aef4bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0306-4522(92)90226-R$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,4025,27928,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5478104$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1354337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klancnik, J.M.</creatorcontrib><creatorcontrib>Cuénod, M.</creatorcontrib><creatorcontrib>Gähwiler, B.H.</creatorcontrib><creatorcontrib>Jiang, Z.P.</creatorcontrib><creatorcontrib>Do, K.Q.</creatorcontrib><title>Release of endogenous amino acids, including homocysteic acid and cysteine sulphinic acid, from rat hippocampal slices evoked by electrical stimulation of Schaffer collateral-commissural fibres</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>This study examined the release of endogenous amino acids from acute hippocampal slices, upon stimulation of the Schaffer collateral-commissural fibres. One-minute samples of superfusate were collected via a cannula placed over the CA1 stratum radiatum, and were analysed by reversed-phase high performance liquid chromatography. Evoked potentials were recorded to ascertain stimulation efficacy. Four minutes of continuous 50 Hz stimulation produced a tetrodotoxin-sensitive release of aspartate and glycine in the second minute of stimulation, as well as a tetrodotoxin-sensitive release of cysteine sulphinic acid, during stimulation and of homocysteic acid, following stimulation. Such 50 Hz stimulation also produced a tetrodotoxin-insensitive decrease in methionine levels, but no significant changes in any of the other 15 amino acids measured. Four minutes of continuous 1 Hz stimulation produced no changes in the levels of any of the amino acids measured, but four 600-ms trains of 100 Hz stimulation, which, unlike the 1 Hz stimulation, produced long-term potentiation, resulted in significant increases in levels of cysteine sulphinic acid and homocysteic acid, but not of any of the other amino acids measured.
These results suggest that aspartate, glycine, homocysteic acid, and cysteine sulphinic acid play a role in synaptic transmission in the Schaffer collateral-commissural fibres, and that cysteine sulphinic acid and homocysteic acid may be released specifically by high-frequency stimulation.</description><subject>ACSF</subject><subject>Amino Acids - metabolism</subject><subject>analysis of variance</subject><subject>Animals</subject><subject>ANOVA</subject><subject>artificial cerebrospinal fluid</subject><subject>Aspartic Acid - metabolism</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>CSA</subject><subject>Cysteine - analogs & derivatives</subject><subject>Cysteine - metabolism</subject><subject>cysteine sulphinic acid</subject><subject>EAA</subject><subject>Electric Stimulation</subject><subject>Evoked Potentials</subject><subject>excitatory amino acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Glutamates - metabolism</subject><subject>Glutamic Acid</subject><subject>Glycine - metabolism</subject><subject>HCA</subject><subject>high-performance liquid chromatography</subject><subject>Hippocampus - physiology</subject><subject>homocysteic acid</subject><subject>Homocysteine - analogs & derivatives</subject><subject>Homocysteine - metabolism</subject><subject>HPLC</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>long-term potentiation</subject><subject>LTP</subject><subject>Male</subject><subject>Methionine - metabolism</subject><subject>N-methyl- d-aspartate</subject><subject>Nerve Fibers - physiology</subject><subject>Neurotransmitter Agents</subject><subject>NMDA</subject><subject>Pyramidal Tracts - physiology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>SAA</subject><subject>sulphur-containing excitatory amino acids</subject><subject>tetrodotoxin</subject><subject>Time Factors</subject><subject>TTX</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUV2L1TAQLaKs19V_oJAHEYWtJm3aNC-CLH7BgnDV55BOJnujbdJN2oX78_xnpvayvmkIzDDnzMxhTlE8ZfQ1o6x9Q2valrypqpeyeiVpVbXl_l6xY52oS9Fwfr_Y3VEeFo9S-kHza3h9VpyxOsda7IpfexxQJyTBEvQmXKMPSyJ6dD4QDc6kC-I8DItx_pocwhjgmGZ08Ack2huyFTyStAzTwfkTdkFsDCOJeiYHN00B9DjpgaTBASaCt-EnGtIfSd4Pc3SwYrMbl0HPLvhVz1c4aGsxEghDrmLUQwlhHF1KS86JdX3E9Lh4YPWQ8MkpnhffP7z_dvmpvPry8fPlu6sSOKvmstdc9oCaV1R2jdAgRU6pNr2QUjeWVpw20BoJIKCruTG9BWFo22m0vIf6vHixzZ1iuFkwzSoLAczKPOaTKVGzinHa_pfI2qpuBJOZyDcixJBSRKum6EYdj4pRtVqsVv_U6p-S-a8Wq31ue3aav_Qjmr9Nm6cZf37CdcpXtVF7cOmO1nDRMcoz7e1Gw3y0W4dRJXDoAY2L2RFlgvu3jt9njMgZ</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Klancnik, J.M.</creator><creator>Cuénod, M.</creator><creator>Gähwiler, B.H.</creator><creator>Jiang, Z.P.</creator><creator>Do, K.Q.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Release of endogenous amino acids, including homocysteic acid and cysteine sulphinic acid, from rat hippocampal slices evoked by electrical stimulation of Schaffer collateral-commissural fibres</title><author>Klancnik, J.M. ; Cuénod, M. ; Gähwiler, B.H. ; Jiang, Z.P. ; Do, K.Q.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-ba49bcea4209857ac974200adb799a5f02405c6d9cc7c834ddbfc7d068aef4bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACSF</topic><topic>Amino Acids - metabolism</topic><topic>analysis of variance</topic><topic>Animals</topic><topic>ANOVA</topic><topic>artificial cerebrospinal fluid</topic><topic>Aspartic Acid - metabolism</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>CSA</topic><topic>Cysteine - analogs & derivatives</topic><topic>Cysteine - metabolism</topic><topic>cysteine sulphinic acid</topic><topic>EAA</topic><topic>Electric Stimulation</topic><topic>Evoked Potentials</topic><topic>excitatory amino acid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Glutamates - metabolism</topic><topic>Glutamic Acid</topic><topic>Glycine - metabolism</topic><topic>HCA</topic><topic>high-performance liquid chromatography</topic><topic>Hippocampus - physiology</topic><topic>homocysteic acid</topic><topic>Homocysteine - analogs & derivatives</topic><topic>Homocysteine - metabolism</topic><topic>HPLC</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>long-term potentiation</topic><topic>LTP</topic><topic>Male</topic><topic>Methionine - metabolism</topic><topic>N-methyl- d-aspartate</topic><topic>Nerve Fibers - physiology</topic><topic>Neurotransmitter Agents</topic><topic>NMDA</topic><topic>Pyramidal Tracts - physiology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>SAA</topic><topic>sulphur-containing excitatory amino acids</topic><topic>tetrodotoxin</topic><topic>Time Factors</topic><topic>TTX</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klancnik, J.M.</creatorcontrib><creatorcontrib>Cuénod, M.</creatorcontrib><creatorcontrib>Gähwiler, B.H.</creatorcontrib><creatorcontrib>Jiang, Z.P.</creatorcontrib><creatorcontrib>Do, K.Q.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klancnik, J.M.</au><au>Cuénod, M.</au><au>Gähwiler, B.H.</au><au>Jiang, Z.P.</au><au>Do, K.Q.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Release of endogenous amino acids, including homocysteic acid and cysteine sulphinic acid, from rat hippocampal slices evoked by electrical stimulation of Schaffer collateral-commissural fibres</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>1992</date><risdate>1992</risdate><volume>49</volume><issue>3</issue><spage>557</spage><epage>570</epage><pages>557-570</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>This study examined the release of endogenous amino acids from acute hippocampal slices, upon stimulation of the Schaffer collateral-commissural fibres. One-minute samples of superfusate were collected via a cannula placed over the CA1 stratum radiatum, and were analysed by reversed-phase high performance liquid chromatography. Evoked potentials were recorded to ascertain stimulation efficacy. Four minutes of continuous 50 Hz stimulation produced a tetrodotoxin-sensitive release of aspartate and glycine in the second minute of stimulation, as well as a tetrodotoxin-sensitive release of cysteine sulphinic acid, during stimulation and of homocysteic acid, following stimulation. Such 50 Hz stimulation also produced a tetrodotoxin-insensitive decrease in methionine levels, but no significant changes in any of the other 15 amino acids measured. Four minutes of continuous 1 Hz stimulation produced no changes in the levels of any of the amino acids measured, but four 600-ms trains of 100 Hz stimulation, which, unlike the 1 Hz stimulation, produced long-term potentiation, resulted in significant increases in levels of cysteine sulphinic acid and homocysteic acid, but not of any of the other amino acids measured.
These results suggest that aspartate, glycine, homocysteic acid, and cysteine sulphinic acid play a role in synaptic transmission in the Schaffer collateral-commissural fibres, and that cysteine sulphinic acid and homocysteic acid may be released specifically by high-frequency stimulation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1354337</pmid><doi>10.1016/0306-4522(92)90226-R</doi><tpages>14</tpages></addata></record> |
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| ispartof | Neuroscience, 1992, Vol.49 (3), p.557-570 |
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| subjects | ACSF Amino Acids - metabolism analysis of variance Animals ANOVA artificial cerebrospinal fluid Aspartic Acid - metabolism Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors CSA Cysteine - analogs & derivatives Cysteine - metabolism cysteine sulphinic acid EAA Electric Stimulation Evoked Potentials excitatory amino acid Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - metabolism Glutamates - metabolism Glutamic Acid Glycine - metabolism HCA high-performance liquid chromatography Hippocampus - physiology homocysteic acid Homocysteine - analogs & derivatives Homocysteine - metabolism HPLC In Vitro Techniques Kinetics long-term potentiation LTP Male Methionine - metabolism N-methyl- d-aspartate Nerve Fibers - physiology Neurotransmitter Agents NMDA Pyramidal Tracts - physiology Rats Rats, Inbred Strains SAA sulphur-containing excitatory amino acids tetrodotoxin Time Factors TTX Vertebrates: nervous system and sense organs |
| title | Release of endogenous amino acids, including homocysteic acid and cysteine sulphinic acid, from rat hippocampal slices evoked by electrical stimulation of Schaffer collateral-commissural fibres |
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