Alpha satellite DNAs on chromosomes 10 and 12 are both members of the dimeric suprachromosomal subfamily, but display little identity at the nucleotide sequence level
We have investigated the organization and complexity of alpha satellite DNA on chromosomes 10 and 12 by restriction endonuclease mapping, in situ hybridization (ISH), and DNA-sequencing methods. Alpha satellite DNA on both chromosomes displays a basic dimeric organization, revealed as a 6- and an 8-...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1992-08, Vol.13 (4), p.1125-1132 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Looijenga, L.H.J. Oosterhuis, J.W. Smit, V.T.H.B.M. Wessels, J.W. Mollevanger, P. Devilee, P. |
| description | We have investigated the organization and complexity of alpha satellite DNA on chromosomes 10 and 12 by restriction endonuclease mapping,
in situ hybridization (ISH), and DNA-sequencing methods. Alpha satellite DNA on both chromosomes displays a basic dimeric organization, revealed as a 6- and an 8-mer higher-order repeat (HOR) unit on chromosome 10 and as an 8-mer HOR on chromosome 12. While these HORs show complete chromosome specificity under high-stringency ISH conditions, they recognize an identical set of chromosomes under lower stringencies. At the nucleotide sequence level, both chromosome 10 HORs are 50% identical to the HOR on chromosome 12 and to all other alpha satellite DNA sequences from the
in situ cross-hybridizing chromosomes, with the exception of chromosome 6. An 80% identity between chromosome 6- and chromosome 10-derived alphoid sequences was observed. These data suggest that the alphoid DNA on chromosomes 6 and 10 may represent a distinct subclass of the dimeric subfamily. These sequences are proposed to be present, along with the more typical dimeric alpha satellite sequences, on a number of different human chromosomes. |
| doi_str_mv | 10.1016/0888-7543(92)90027-P |
| format | Article |
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in situ hybridization (ISH), and DNA-sequencing methods. Alpha satellite DNA on both chromosomes displays a basic dimeric organization, revealed as a 6- and an 8-mer higher-order repeat (HOR) unit on chromosome 10 and as an 8-mer HOR on chromosome 12. While these HORs show complete chromosome specificity under high-stringency ISH conditions, they recognize an identical set of chromosomes under lower stringencies. At the nucleotide sequence level, both chromosome 10 HORs are 50% identical to the HOR on chromosome 12 and to all other alpha satellite DNA sequences from the
in situ cross-hybridizing chromosomes, with the exception of chromosome 6. An 80% identity between chromosome 6- and chromosome 10-derived alphoid sequences was observed. These data suggest that the alphoid DNA on chromosomes 6 and 10 may represent a distinct subclass of the dimeric subfamily. These sequences are proposed to be present, along with the more typical dimeric alpha satellite sequences, on a number of different human chromosomes.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/0888-7543(92)90027-P</identifier><identifier>PMID: 1505948</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Base Sequence ; Biological and medical sciences ; Blotting, Southern ; Chromosomes, Human, Pair 10 ; Chromosomes, Human, Pair 12 ; DNA, Satellite - genetics ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Humans ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Repetitive Sequences, Nucleic Acid ; Sequence Homology, Nucleic Acid</subject><ispartof>Genomics (San Diego, Calif.), 1992-08, Vol.13 (4), p.1125-1132</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-786d1de227a29ad5a73c7d21c14fd565e1073869ffeb1cfa924f51cc7907faa23</citedby><cites>FETCH-LOGICAL-c367t-786d1de227a29ad5a73c7d21c14fd565e1073869ffeb1cfa924f51cc7907faa23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0888-7543(92)90027-P$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5499983$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1505948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Looijenga, L.H.J.</creatorcontrib><creatorcontrib>Oosterhuis, J.W.</creatorcontrib><creatorcontrib>Smit, V.T.H.B.M.</creatorcontrib><creatorcontrib>Wessels, J.W.</creatorcontrib><creatorcontrib>Mollevanger, P.</creatorcontrib><creatorcontrib>Devilee, P.</creatorcontrib><title>Alpha satellite DNAs on chromosomes 10 and 12 are both members of the dimeric suprachromosomal subfamily, but display little identity at the nucleotide sequence level</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>We have investigated the organization and complexity of alpha satellite DNA on chromosomes 10 and 12 by restriction endonuclease mapping,
in situ hybridization (ISH), and DNA-sequencing methods. Alpha satellite DNA on both chromosomes displays a basic dimeric organization, revealed as a 6- and an 8-mer higher-order repeat (HOR) unit on chromosome 10 and as an 8-mer HOR on chromosome 12. While these HORs show complete chromosome specificity under high-stringency ISH conditions, they recognize an identical set of chromosomes under lower stringencies. At the nucleotide sequence level, both chromosome 10 HORs are 50% identical to the HOR on chromosome 12 and to all other alpha satellite DNA sequences from the
in situ cross-hybridizing chromosomes, with the exception of chromosome 6. An 80% identity between chromosome 6- and chromosome 10-derived alphoid sequences was observed. These data suggest that the alphoid DNA on chromosomes 6 and 10 may represent a distinct subclass of the dimeric subfamily. These sequences are proposed to be present, along with the more typical dimeric alpha satellite sequences, on a number of different human chromosomes.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Chromosomes, Human, Pair 10</subject><subject>Chromosomes, Human, Pair 12</subject><subject>DNA, Satellite - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuLFDEUhYMoYzv6DxSyEFGwNEk9UtkMNOMTBp2FrsOt5IaOpB4mqYH-Q_5O09NNu3MVuPc754ZzCHnO2TvOePee9X1fybapXyvxRjEmZHX7gGw461XVd033kGzOyGPyJKVfjDFV9-KCXPCWtarpN-TPNiw7oAkyhuAz0g_ftonOEzW7OI9zmkdMlDMKk6VcUIhIhznv6IjjgLGQjuYdUutHjN7QtC4RzlIIZTA4GH3Yv6XDmguXlgB7Wk7lgNRbnLLPewr53mZaTcA5lzFN-HvFySANeIfhKXnkICR8dnovyc9PH39cf6luvn_-er29qUzdyVzJvrPcohAShALbgqyNtIIb3jjbdi1yJuu-U87hwI0DJRrXcmOkYtIBiPqSvDr6LnEu91PWo0-mRAMTzmvSsuZc1UIVsDmCJs4pRXR6iX6EuNec6UM9-pC9PmSvldD39ejbIntx8l-HEe0_0bGPsn952kMyEFyEyfh0xtpGKdXXBbs6YliyuPMYdTL-kJb1EU3Wdvb__8dfyAyu5g</recordid><startdate>199208</startdate><enddate>199208</enddate><creator>Looijenga, L.H.J.</creator><creator>Oosterhuis, J.W.</creator><creator>Smit, V.T.H.B.M.</creator><creator>Wessels, J.W.</creator><creator>Mollevanger, P.</creator><creator>Devilee, P.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199208</creationdate><title>Alpha satellite DNAs on chromosomes 10 and 12 are both members of the dimeric suprachromosomal subfamily, but display little identity at the nucleotide sequence level</title><author>Looijenga, L.H.J. ; Oosterhuis, J.W. ; Smit, V.T.H.B.M. ; Wessels, J.W. ; Mollevanger, P. ; Devilee, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-786d1de227a29ad5a73c7d21c14fd565e1073869ffeb1cfa924f51cc7907faa23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Chromosomes, Human, Pair 10</topic><topic>Chromosomes, Human, Pair 12</topic><topic>DNA, Satellite - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Looijenga, L.H.J.</creatorcontrib><creatorcontrib>Oosterhuis, J.W.</creatorcontrib><creatorcontrib>Smit, V.T.H.B.M.</creatorcontrib><creatorcontrib>Wessels, J.W.</creatorcontrib><creatorcontrib>Mollevanger, P.</creatorcontrib><creatorcontrib>Devilee, P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Looijenga, L.H.J.</au><au>Oosterhuis, J.W.</au><au>Smit, V.T.H.B.M.</au><au>Wessels, J.W.</au><au>Mollevanger, P.</au><au>Devilee, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alpha satellite DNAs on chromosomes 10 and 12 are both members of the dimeric suprachromosomal subfamily, but display little identity at the nucleotide sequence level</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1992-08</date><risdate>1992</risdate><volume>13</volume><issue>4</issue><spage>1125</spage><epage>1132</epage><pages>1125-1132</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>We have investigated the organization and complexity of alpha satellite DNA on chromosomes 10 and 12 by restriction endonuclease mapping,
in situ hybridization (ISH), and DNA-sequencing methods. Alpha satellite DNA on both chromosomes displays a basic dimeric organization, revealed as a 6- and an 8-mer higher-order repeat (HOR) unit on chromosome 10 and as an 8-mer HOR on chromosome 12. While these HORs show complete chromosome specificity under high-stringency ISH conditions, they recognize an identical set of chromosomes under lower stringencies. At the nucleotide sequence level, both chromosome 10 HORs are 50% identical to the HOR on chromosome 12 and to all other alpha satellite DNA sequences from the
in situ cross-hybridizing chromosomes, with the exception of chromosome 6. An 80% identity between chromosome 6- and chromosome 10-derived alphoid sequences was observed. These data suggest that the alphoid DNA on chromosomes 6 and 10 may represent a distinct subclass of the dimeric subfamily. These sequences are proposed to be present, along with the more typical dimeric alpha satellite sequences, on a number of different human chromosomes.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1505948</pmid><doi>10.1016/0888-7543(92)90027-P</doi><tpages>8</tpages></addata></record> |
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| ispartof | Genomics (San Diego, Calif.), 1992-08, Vol.13 (4), p.1125-1132 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Base Sequence Biological and medical sciences Blotting, Southern Chromosomes, Human, Pair 10 Chromosomes, Human, Pair 12 DNA, Satellite - genetics Fundamental and applied biological sciences. Psychology Genes. Genome Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid |
| title | Alpha satellite DNAs on chromosomes 10 and 12 are both members of the dimeric suprachromosomal subfamily, but display little identity at the nucleotide sequence level |
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