Determination of Nonderivatized para-Hydroxylated Metabolites of Diazepam in Biological Fluids with a GC Megabore Column System

A method was developed using gas chromatography (GC) and a Megabore column system capable of simultaneous detection of diazapam, N-desmethyldiazepam, temazepam, oxazepam, and their para-hydroxylated metabolites. This method does not require derivatization of para-hydroxylated metabolites. Standard c...

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Veröffentlicht in:	Journal of analytical toxicology 1992-07, Vol.16 (4), p.236-239
Hauptverfasser:	Beischlag, T.V., Inaba, T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animals Biological and medical sciences Chromatography, Gas Diazepam - metabolism Diazepam - urine General pharmacology Hydroxylation Male Medical sciences Nordazepam - urine Oxazepam - urine Pharmacology. Drug treatments Rats Rats, Inbred Strains Temazepam - urine
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container_issue	4
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container_title	Journal of analytical toxicology
container_volume	16
creator	Beischlag, T.V. Inaba, T.
description	A method was developed using gas chromatography (GC) and a Megabore column system capable of simultaneous detection of diazapam, N-desmethyldiazepam, temazepam, oxazepam, and their para-hydroxylated metabolites. This method does not require derivatization of para-hydroxylated metabolites. Standard curves for pure reference compounds were linear, with the minimum detectable concentration of diazepam and its metabolites as low as 0.13 ng/injection.
doi_str_mv	10.1093/jat/16.4.236
format	Article
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source	Oxford University Press Journals Digital Archive legacy; MEDLINE; Alma/SFX Local Collection
subjects	Analysis Animals Biological and medical sciences Chromatography, Gas Diazepam - metabolism Diazepam - urine General pharmacology Hydroxylation Male Medical sciences Nordazepam - urine Oxazepam - urine Pharmacology. Drug treatments Rats Rats, Inbred Strains Temazepam - urine
title	Determination of Nonderivatized para-Hydroxylated Metabolites of Diazepam in Biological Fluids with a GC Megabore Column System
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